Considerations in Making Stereo Micrographs With the Scanning Electron Microscope

Author(s):  
E. R. Walter

The information obtainable with the scanning electron microscope can often be increased severalfold through the use of stereomicrograph pairs. Not only is the detail which can be observed the equivalent of a 2X increase in magnification over that apparent in a single micrograph, but, threedimensional spatial relationships are more accurately preserved. This is especially true where protruding or pyramided fine structure and/or gross reentrance exists. Since stereo micrographs are conveniently obtained with most scanning electron microscopes, it is generally desirable to add the additional information they offer whenever the three-dimensional relationships present in the specimen'are not readily apparent.

2014 ◽  
Vol 32 (2) ◽  
pp. 275-278
Author(s):  
Joanna Z. Kadłubowska ◽  
Ewa Kalinowska-Kucharska

Several year long investigations of the developmental cycle of <i>Microsphaera palczewskii</i> occurring on the leaves of <i>Caragana arborescens</i> in Central Poland are reported. The material was studied with light and scanning electron microscopes. The scanning microscopy micrographs of the clistothecia and appendages presented in this report are the first micrographs of this species.


2018 ◽  
Vol 69 (1) ◽  
pp. 24-31
Author(s):  
Khaled S. Hatamleh ◽  
Qais A. Khasawneh ◽  
Adnan Al-Ghasem ◽  
Mohammad A. Jaradat ◽  
Laith Sawaqed ◽  
...  

Abstract Scanning Electron Microscopes are extensively used for accurate micro/nano images exploring. Several strategies have been proposed to fine tune those microscopes in the past few years. This work presents a new fine tuning strategy of a scanning electron microscope sample table using four bar piezoelectric actuated mechanisms. The introduced paper presents an algorithm to find all possible inverse kinematics solutions of the proposed mechanism. In addition, another algorithm is presented to search for the optimal inverse kinematic solution. Both algorithms are used simultaneously by means of a simulation study to fine tune a scanning electron microscope sample table through a pre-specified circular or linear path of motion. Results of the study shows that, proposed algorithms were able to minimize the power required to drive the piezoelectric actuated mechanism by a ratio of 97.5% for all simulated paths of motion when compared to general non-optimized solution.


1997 ◽  
Vol 3 (S2) ◽  
pp. 1243-1244 ◽  
Author(s):  
Raynald Gauvin ◽  
Steve Yue

The observation of microstructural features smaller than 300 nm is generally performed using Transmission Electron Microscopy (TEM) because conventional Scanning Electron Microscopes (SEM) do not have the resolution to image such small phases. Since the early 1990’s, a new generation of microscopes is now available on the market. These are the Field Emission Gun Scanning Electron Microscope with a virtual secondary electron detector. The field emission gun gives a higher brightness than those obtained using conventional electron filaments allowing enough electrons to be collected to operate the microscope with incident electron energy, E0, below 5 keV with probe diameter smaller than 5 nm. At 1 keV, the electron range is 60 nm in aluminum and 10 nm in iron (computed using the CASINO program). Since the electron beam diameter is smaller than 5 nm at 1 keV, the resolution of these microscopes becomes closer to that of TEM.


1998 ◽  
Vol 4 (S2) ◽  
pp. 896-897
Author(s):  
W. A. Lambe ◽  
P.M. Brady

The variety of instrumentation available to the researcher today can be overwhelming and confusing. Scanning Electron Microscopes (“SEM's) are no exception, and choosing one can often serve as an exercise in dealing with complexity. First time purchasers are most at risk, being subject to a barrage of information that attempts to sway the purchaser in one direction or the other. As a result, one can sometimes be drawn to the details of the latest “high end” performance parameter, while overlooking the basics. At its worst, the selection process can degrade to one of vague guesswork with little hard data to serve as a compass.By applying a methodical approach to define your individual requirements, carefully designed tests of actual instruments, and discussions with your collaborators, potential and experienced users, one can begin to ensure a successful selection process.


1998 ◽  
Vol 44 ◽  
pp. 331-347 ◽  
Author(s):  
K. C. A. Smith

Charles Oatley made three outstanding contributions to the engineering sciences: he was one of the brilliant team that developed radar in Britain during the Second World War; he revolutionized the teaching of electronics at Cambridge University; and he developed the scanning electron microscope. It is for the last of these that he will be chiefly remembered. He stands with Manfred von Ardenne as one of the two great pioneers of scanning electron microscopy His involvement with the instrument began shortly after the war when, fresh from his experience in the development of radar, he perceived that new techniques could be brought to bear which would overcome some of the fundamental problems encountered by von Ardenne in his pre–war research. Oatley's work led directly to the launch of the world's first series production instrument—the Stereoscan—in 1965. Thousands of scanning electron microscopes have since been manufactured and are to be found in practically every research laboratory in the world. The striking three–dimensional images of microscopic organisms produced have been used to illustrate countless newspaper and magazine articles, as well as scientific research papers, giving the general public a new perspective and appreciation of the world that lies beyond the resolution of the human eye. The scanning electron microscope is, arguably, the single most important scientific instrument of the post-war era.


Author(s):  
Mark H. Ransick ◽  
Chadwick D. Barklay

Most manufacturers of scanning electron microscopes (SEM) now offer models that display an image digitally. This holds many advantages, including the ability to store the image on a disk and perform image analysis on the sample. Most SEMs in service, however, produce only an analog video output; they do not have the ability to digitize the image. Film is the only method of storing images.Consequentially, film is a significant portion of every microscopy laboratory’s budget. Completely eliminating the use of film from use is not practical. There will always be the need to examine a hard copy of the image; many programs require duplicate copies of each image generated; and it is sound practice to keep a copy of each image on file. By archiving digital images to an inexpensive media, the amount of film used or the time devoted to processing negatives can be greatly reduced.By using personal computers (PCs)s, with a digitizing board and analog to digital (A/D) board, it is possible to construct a relatively low cost digitizing system for any SEM.


Author(s):  
Stuart McKeman

Several recent advances have had a major potential impact on the microscopy of ceramic materials. The ability of modern scanning electron microscopes to image uncoated materials, at low voltage for example, whilst still maintaining high resolution should make possible a wide variety of experiments that were hitherto impossible to contemplate. This ability to look at the unmodified surface of a ceramic enables iterative or dynamic experiments to be done with a lot more confidence in the results than has been possible before. A second advance has been the introduction of microscopes capable of operating at higher pressures than was previously possible. This makes possible the ability to image specimens in a variety of different environments. The environmental scanning electron microscope (ESEM) exploits of both of these novel areas. The aim of this review is to highlight areas where the unique capabilities of the ESEM may be applied to advance our understanding of ceramics.


2001 ◽  
Vol 7 (S2) ◽  
pp. 494-495
Author(s):  
M. T. Postek ◽  
A. E. Vladar ◽  
N.-F. Zhang ◽  
R. Larrabee

All scanning electron microscopes, whether they are in laboratory or on the production line, slowly lose performance as the instrument is used. This loss of performance is due to a whole array of contributing factors including misalignments, contamination and increases in source diameter. Identifying a loss in “sharpness” easily recognizes this performance decrease. Reference Material 8091 (RM 8091) is intended primarily for use in routinely checking the sharpness performance of scanning electron microscopes (Fig. 1). RM 8091 is designed to be used in conjunction with the SPECTEL SEM Monitor Program, the NIST Kurtosis program,3 or the University of Tennessee SMART program. RM 8091 is supplied as a small, approximately 2 mm x 2 mm diced semiconductor chip. This sample is capable of being mounted directly on to a wafer, wafer piece or specimen stub for insertion into a laboratory scanning electron microscope or wafer inspection scanning electron microscope.


Author(s):  
Marc H. Peeters

Over the past ten years, the combination of scanning electron microscopy and energy dispersive analysis has proven to be a good and reliable tool for elemental characterization of the different components of gunshot residue. The technique evolved from a qualitative technique, involving a large amount of operator involvement, to a higher degree of quantification and automation allowing unattended analysis of gunshot residue samples. The PHAX-Scan system is geared to automated unattended analysis, particularly suited to applications such as gunshot-residue analysis.PHAX-Scan is a system based on a Philips scanning electron microscope and an EDAX PV9900 energy dispersive microanalyser in combination with highly optimised software. The system makes use of the dual processor structure of the EDAX analyser and of the bus structure of the Philips scanning electron microscopes. It is designed such that it performs a fully quantitative data reduction, including background subtraction, peak deconvolution, no-standards k-ratio determination and Z*A*F correction in typically 100 milliseconds.


2012 ◽  
Vol 20 (5) ◽  
pp. 10-15 ◽  
Author(s):  
David C. Joy

Over the past fifty years the scanning electron microscope (SEM) has established itself as the most versatile and productive tool for imaging and microanalysis in many areas of science and technology, and some seventy-thousand instruments generate millions of micrographs every day. Scanning electron microscopes do, however, have one fundamental limitation in that the only experimental variable available to the operator is the choice of the accelerating voltage. Although the ability to vary beam energy is both necessary and important, it is an unfortunate fact that changing the beam energy also alters many aspects of performance: imaging resolution, relative strength of different signal components, depth of beam penetration, capabilities of the various analytical systems, and the severity of charging and beam-induced damage. This makes it difficult or impossible to optimize the interaction of interest.


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