Ultrastructure of adrenal reticularis and Sertoli cells in sterile male mice

1983 ◽  
Vol 41 ◽  
pp. 770-771
Author(s):  
Yvonne White ◽  
Terrell Hoage ◽  
Jack Turner ◽  
Jack Bishop
Keyword(s):  
Sertoli Cells ◽  
Serum Testosterone ◽  
Testicular Tissue ◽  
High Serum ◽  
Hormonal Control ◽  
Chromosomal Translocations ◽  
Sterile Male ◽  
Male Mice ◽  
Zona Reticularis ◽  
Low Serum

Heterozygous translocations have been established as a cause of male infertility in mice. Previous studies did not establish a relationship between translocations and hormonal control of fertility in males. We examined the adrenal-zona-reticularis-cell/Sertoli cell hormonal axis in sterile male mice that had chromosomal translocations induced by triethylenemel amine (TEM). Out of 397 male offspring of TEM treated CD1 males and F1 (C57BL/6J x BALB/c) females 26 were found to be infertile and confirmed as translocation carriers by cytogenetic analysis of testicular tissue acquired by unilateral orchiectomy at 11 - 13 months of age. Radioimmunoassay of blood serum testosterone collected between 14 and 18 months of age showed the sterile males to vary from 0.96 ± 0.41 to 10.55 ± 1.65 ng/ml compared to 3.17 ± 0.55 ng/ml in fertile controls.Adrenal and testicular tissues were collected from ether-anesthetized animals at 18 months of age. Two or three animals were selected to represent each group: non-translocation controls, and translocation groups with high serum testosterone (HT), medial serum testosterone (MT) and low serum testosterone (LT).

scholarly journals Low Serum Testosterone and High Serum Estradiol Associate With Lower Extremity Peripheral Arterial Disease in Elderly Men

2007 ◽  
Vol 50 (11) ◽  
pp. 1070-1076 ◽  
Author(s):  
Åsa Tivesten ◽  
Dan Mellström ◽  
Hans Jutberger ◽  
Björn Fagerberg ◽  
Bodil Lernfelt ◽  
...  
Keyword(s):  
Peripheral Arterial Disease ◽  
Lower Extremity ◽  
Serum Testosterone ◽  
Arterial Disease ◽  
High Serum ◽  
Serum Estradiol ◽  
Elderly Men ◽  
Peripheral Arterial ◽  
Low Serum

Hepatic steatosis is associated with low serum testosterone and high serum DHEAS levels in men

2009 ◽  
Vol 33 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Henry Völzke ◽  
Nicole Aumann ◽  
Alexander Krebs ◽  
Matthias Nauck ◽  
Antje Steveling ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Serum Testosterone ◽  
High Serum ◽  
Low Serum

Fibronexus-Like Adhesion Sites are Present at the Invasive Zones of Human Epidermoid Carcinomas

1982 ◽  
Vol 40 ◽  
pp. 106-109
Author(s):  
Irwin I. Singer
Keyword(s):  
Cell Cycle ◽  
Serum Concentration ◽  
Substrate Binding ◽  
High Serum ◽  
Adhesion Sites ◽  
Substrate Adhesion ◽  
Focal Contacts ◽  
Adhesion Complex ◽  
Low Serum

Our previous results indicate that two types of fibronectin-cytoskeletal associations may be formed at the fibroblast surface: dorsal matrixbinding fibronexuses generated in high serum (5% FBS) cultures, and ventral substrate-adhering units formed in low serum (0.3% FBS) cultures. The substrate-adhering fibronexus consists of at least vinculin (VN) and actin in its cytoplasmic leg, and fibronectin (FN) as one of its major extracellular components. This substrate-adhesion complex is localized in focal contacts, the sites of closest substratum approach visualized with interference reflection microscopy, which appear to be the major points of cell-tosubstrate adhesion. In fibroblasts, the latter substrate-binding complex is characteristic of cultures that are arrested at the G1 phase of the cell cycle due to the low serum concentration in their medium. These arrested fibroblasts are very well spread, flattened, and immobile.

scholarly journals Evaluation of the nature and etiologies of risk factors for diaphyseal atypical femoral fractures

2021 ◽  
Author(s):  
Hiroyuki Tsuchie ◽  
Naohisa Miyakoshi ◽  
Yuji Kasukawa ◽  
Koji Nozaka ◽  
Kimio Saito ◽  
...  
Keyword(s):  
Risk Factors ◽  
Multivariate Analyses ◽  
Femoral Fractures ◽  
Japanese Patients ◽  
High Serum ◽  
Control Group ◽  
Atypical Femoral Fractures ◽  
Factors Affecting ◽  
Lateral Curvature ◽  
Low Serum

Objectives: Differences in the mechanisms of subtrochanteric and diaphyseal atypical femoral fractures (AFFs) have been speculated in studies that have analyzed differences in the patients’ backgrounds. However, the etiologies of each type of AFF have not been investigated in detail. Therefore, this study aimed to investigate the nature and etiologies of the risk factors for diaphyseal AFFs. Materials and Methods: Eighty consecutive Japanese patients with 91 diaphyseal AFFs (the AFF group) and 110 age-matched female patients with osteoporosis (the non-AFF control group) were included. Their clinical data were compared and the factors affecting AFFs were investigated. Furthermore, the etiologies of the risk factors for diaphyseal AFFs were examined. Results: Multivariate analysis revealed that femoral serrated changes, bisphosphonate or denosumab usage, and lateral and anterior femoral curvatures were the risk factors for diaphyseal AFFs (p<0.0011, p=0.0137, and p<0.0001, respectively). Multivariate analyses also revealed that serrated changes and low serum 25(OH)D levels affected the lateral curvature (p=0.0088 and 0.0205, respectively), while serrated changes affected the anterior curvature (p=0.0006); each significantly affected the femoral curvature. In addition, a high serum calcium (Ca) level, lateral femoral curvature, and anterior femoral curvature were the predictors of serrated changes (p=0.0146, 0.0002, and 0.0098, respectively). Conclusion: The risk factors for diaphyseal AFFs were bone resorption inhibitor usage, a strong femoral curvature, and serrated changes. A low serum 25(OH)D level and serrated changes are the risk factors for lateral curvature, while a high serum Ca level is a risk factor for serrated changes.

scholarly journals Kindlin-2 in Sertoli cells is essential for testis development and male fertility in mice

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Xiaochun Chi ◽  
Weiwei Luo ◽  
Jiagui Song ◽  
Bing Li ◽  
Tiantian Su ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Male Fertility ◽  
Nuclear Translocation ◽  
Hippo Pathway ◽  
Reproductive Organs ◽  
Male Reproduction ◽  
Barrier Structure ◽  
Male Mice ◽  
Sperm Development

AbstractKindlin-2 is known to play important roles in the development of mesoderm-derived tissues including myocardium, smooth muscle, cartilage and blood vessels. However, nothing is known for the role of Kindlin-2 in mesoderm-derived reproductive organs. Here, we report that loss of Kindlin-2 in Sertoli cells caused severe testis hypoplasia, abnormal germ cell development and complete infertility in male mice. Functionally, loss of Kindlin-2 inhibits proliferation, increases apoptosis, impairs phagocytosis in Sertoli cells and destroyed the integration of blood-testis barrier structure in testes. Mechanistically, Kindlin-2 interacts with LATS1 and YAP, the key components of Hippo pathway. Kindlin-2 impedes LATS1 interaction with YAP, and depletion of Kindlin-2 enhances LATS1 interaction with YAP, increases YAP phosphorylation and decreases its nuclear translocation. For clinical relevance, lower Kindlin-2 expression and decreased nucleus localization of YAP was found in SCOS patients. Collectively, we demonstrated that Kindlin-2 in Sertoli cells is essential for sperm development and male reproduction.

Low serum testosterone levels are predictive of prostate cancer

2011 ◽  
Vol 31 (2) ◽  
pp. 247-252 ◽  
Author(s):  
Luigi Mearini ◽  
Alessandro Zucchi ◽  
Elisabetta Nunzi ◽  
Tommaso Villirillo ◽  
Vittorio Bini ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Serum Testosterone ◽  
Testosterone Levels ◽  
Low Serum

scholarly journals High Serum Testosterone Is Associated With Reduced Risk of Cardiovascular Events in Elderly Men

2011 ◽  
Vol 58 (16) ◽  
pp. 1674-1681 ◽  
Author(s):  
Claes Ohlsson ◽  
Elizabeth Barrett-Connor ◽  
Shalender Bhasin ◽  
Eric Orwoll ◽  
Fernand Labrie ◽  
...  
Keyword(s):  
Cardiovascular Events ◽  
Serum Testosterone ◽  
High Serum ◽  
Elderly Men ◽  
Reduced Risk

Variations in serum T3, rT3, 3,3'-diiodothyronine and 3',5'-diiodothyronine induced by acute myocardial infarction and propranolol

1980 ◽  
Vol 94 (3) ◽  
pp. 341-345 ◽  
Author(s):  
Jens Faber ◽  
Carsten Kirkegaard ◽  
Ib Bo Lumholtz ◽  
Kaj Siersbæk-Nielsen ◽  
Thorkild Friis
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Enzyme System ◽  
Serum Levels ◽  
High Serum ◽  
Early Course ◽  
Deiodinase Activity ◽  
Serum T3 ◽  
Low Serum ◽  
Close Parallelism

Abstract. Serum levels of thyroxine, 3,5,3'-triiodothyronine (T3), 3,3',5'-triiodothyronine (rT3), 3,3'-diiodothyronine (3,3'-T2), 3',5'-diiodothyronine (3',5'-T2) and TSH were measured in two clinical situations which are both known to induce a low serum T3 high serum rT3 syndrome: 1) during the early course of acute myocardial infarction (AMI) and after recovery, and 2) before and during one week's propranolol medication (20 mg 4 times a day). In 10 patients with AMI serum levels of the iodothyronines were unchanged on admission to hospital (in average 6.6 h after onset of symptoms). However, already 24 h after onset of symptoms serum T3 and 3,3'T2 were reduced whereas serum rT3 and 3',5'-T2 were increased. Serum T3 and 3,3'-T2 reached a nadir on day 4 and 3, respectively, whereas serum rT3 and 3',5'-T2 reached peak values 24 h after onset of symptoms. In eight healthy, euthyroid volunteers propranolol medication induced similar changes in iodothyronine concentration as AMI did. However, the alterations were more delayed. Serum T3 decreased slowly reaching statistically significantly reduced values on day 7. Serum rT3 and 3',5'-T2 were significantly enhanced from day 3 and 4, respectively. A close parallelism in alterations of serum T3 and 3,3'-T2 levels was observed. Our data suggest that T3 in the two situations studied is a major precursor for 3,3'-T2 probably as a consequence of reduced 5'-deiodinase activity. It seems possible that the mechanisms affecting the metabolism of the iodothyronines in AMI and during propranolol medication involved the same enzyme system. However, the late appearance of the alterations in serum iodothyronines levels during propranolol medication might indicate different modes of action.

scholarly journals Cognitive effects of aromatase inhibitor therapy in peripubertal boys

2010 ◽  
Vol 163 (1) ◽  
pp. 149-155 ◽  
Author(s):  
M Hero ◽  
S Maury ◽  
E Luotoniemi ◽  
E Service ◽  
L Dunkel
Keyword(s):  
Aromatase Inhibitor ◽  
Short Stature ◽  
Cognitive Performance ◽  
Verbal Memory ◽  
Memory Function ◽  
Serum Testosterone ◽  
High Serum ◽  
Cognitive Effects ◽  
Double Blind

ObjectiveAromatase inhibitors, blockers of oestrogen biosynthesis, have emerged as a new potential treatment modality for boys with short stature. The cognitive effects of such therapy are unknown. In this study, we explored the effects of aromatase inhibition on cognitive performance in peripubertal boys.DesignProspective, double-blind, randomised, placebo-controlled clinical study.MethodsTwenty-eight boys, aged 9.0–14.5 years, with idiopathic short stature were treated with the aromatase inhibitor letrozole (2.5 mg/day) or placebo, for 2 years. During the treatment, the progression of physical signs of puberty and the concentrations of sex hormones were followed up. A selection of cognitive tests, focusing on memory function, was administered to the participants at entry, at 12 months and at 24 months after the start of the treatment.ResultsLetrozole effectively inhibited the conversion of androgen to oestrogen, as indicated by high serum testosterone and low serum oestradiol concentrations in letrozole-treated boys who progressed into puberty. In both the groups, there was a gain in performance during the follow-up period in tests of verbal performance, in most of the tests of visuospatial performance and in some tests of verbal memory. No significant differences between the letrozole- and placebo-treated boys in development of cognitive performance were found in any of the tests during the follow-up period.ConclusionsOur results suggest that blockade of oestrogen biosynthesis with an aromatase inhibitor does not influence cognitive performance in peripubertal males.

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