Quantitative CBED: an overview

1995 ◽  
Vol 53 ◽  
pp. 130-131
Author(s):  
D.M. Bird
Keyword(s):  
Quantitative Analysis ◽  
High Resolution ◽  
Computer Simulations ◽  
Two Dimensional ◽  
Basic Point ◽  
Beam Equations ◽  
The Many ◽  
Resolution Simulation

In this abstract I will focus on one particular topic which has widespread relevance in the quantitative analysis of CBED; namely the role of computer simulations of CBED patterns. The basic point is that with modern, high-powered workstations we can perform highly accurate and detailed simulations of all types of CBED patterns from crystals in a relatively short amount of time. Most CBED simulations are based on diagonalisation of the many-beam equations, and, for example, on a DEC Alpha workstation one can perform this calculation on a 150 × 150 matrix (sufficient for a highly accurate simulation in many systems) in around 1.8s. A full, two-dimensional CBED pattern with, say, 50 orientations across each disc (which is a high-resolution simulation) can then be calculated in less than 1 hour. How can such simulations be used in a quantitative analysis of CBED patterns? I will address this by looking at some specific examples covering various aspects of quantitative CBED.

scholarly journals Objective Center-Finding Algorithm for Tropical Cyclones in Numerical Models

Atmosphere ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 376 ◽  
Author(s):  
Chengwu Zhao ◽  
Junqiang Song ◽  
Hongze Leng ◽  
Juan Zhao
Keyword(s):  
High Resolution ◽  
Tropical Cyclones ◽  
Numerical Models ◽  
Small Scale ◽  
Two Dimensional ◽  
Model Data ◽  
Centroid Algorithm ◽  
Resolution Model ◽  
High Resolution Model ◽  
Resolution Simulation

Precise center-detection of tropical cyclones (TCs) is critical for dynamic analysis in high resolution model data. The existence of both smaller scale perturbations and larger scale circulations could reduce the accuracy of center positioning. In this study, an objective center-finding algorithm is developed based on a two-dimensional Fourier filter and a vorticity centroid algorithm. This proposed algorithm is able to automatically adjust its parameters according to the scale of the target vortex instead of using artificially prescribed parameters in previous research. What’s more, this new algorithm has been optimized and validated by a hundred idealized vortexes with different sizes and small-scale perturbations. A high-resolution simulation of Typhoon Soudelor (2015) was used to evaluate the performance of the new algorithm, and the proposed objective center-finding algorithm was found able to detect a precise and reliable center.

Role of the valence-band mass on the many-body optical properties of a two-dimensional electron gas

1998 ◽  
Vol 249-251 ◽  
pp. 603-606
Author(s):  
H.P van der Meulen ◽  
J Rubio ◽  
J.M Calleja ◽  
C Tejedor ◽  
F Rodriguez ◽  
...  
Keyword(s):  
Optical Properties ◽  
Valence Band ◽  
Electron Gas ◽  
Two Dimensional ◽  
Dimensional Electron ◽  
Many Body ◽  
Two Dimensional Electron Gas ◽  
Dimensional Electron Gas ◽  
The Many

High Resolution Microscopy of Multi-Layered Biological Crystals

1982 ◽  
Vol 40 ◽  
pp. 80-83
Author(s):  
H.A. Cohen ◽  
T.W. Jeng ◽  
W. Chiu
Keyword(s):  
High Resolution ◽  
Low Dose ◽  
Three Dimensional ◽  
Data Interpretation ◽  
Two Dimensional ◽  
Biological Objects ◽  
Density Maps ◽  
Density Map ◽  
Complex Crystal ◽  
High Resolution Microscopy

This tutorial will discuss the methodology of low dose electron diffraction and imaging of crystalline biological objects, the problems of data interpretation for two-dimensional projected density maps of glucose embedded protein crystals, the factors to be considered in combining tilt data from three-dimensional crystals, and finally, the prospects of achieving a high resolution three-dimensional density map of a biological crystal. This methodology will be illustrated using two proteins under investigation in our laboratory, the T4 DNA helix destabilizing protein gp32*I and the crotoxin complex crystal.

On Future Directions in Pathology

1982 ◽  
Vol 40 ◽  
pp. 274-277
Author(s):  
Benjamin F. Trump ◽  
Irene K. Berezesky ◽  
Raymond T. Jones
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Clinical Pathology ◽  
Future Directions ◽  
Diagnostic Pathology ◽  
The Past ◽  
Transmission Electron ◽  
Organ Systems ◽  
The Many

The role of electron microscopy and associated techniques is assured in diagnostic pathology. At the present time, most of the progress has been made on tissues examined by transmission electron microscopy (TEM) and correlated with light microscopy (LM) and by cytochemistry using both plastic and paraffin-embedded materials. As mentioned elsewhere in this symposium, this has revolutionized many fields of pathology including diagnostic, anatomic and clinical pathology. It began with the kidney; however, it has now been extended to most other organ systems and to tumor diagnosis in general. The results of the past few years tend to indicate the future directions and needs of this expanding field. Now, in addition to routine EM, pathologists have access to the many newly developed methods and instruments mentioned below which should aid considerably not only in diagnostic pathology but in investigative pathology as well.

A High Resolution Study of G.P. Zones in Aluminum - 4.2 at % Silver

1982 ◽  
Vol 40 ◽  
pp. 722-723 ◽  
Author(s):  
R. Gronsky
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Computer Simulations ◽  
Structural Characteristics ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Resolution Study

The phenomenon of clustering in Al-Ag alloys has been extensively studied since the early work of Guinierl, wherein the pre-precipitation state was characterized as an assembly of spherical, ordered, silver-rich G.P. zones. Subsequent x-ray and TEM investigations yielded results in general agreement with this model. However, serious discrepancies were later revealed by the detailed x-ray diffraction - based computer simulations of Gragg and Cohen, i.e., the silver-rich clusters were instead octahedral in shape and fully disordered, atleast below 170°C. The object of the present investigation is to examine directly the structural characteristics of G.P. zones in Al-Ag by high resolution transmission electron microscopy.

Tubulin structure at intermediate resolution

1995 ◽  
Vol 53 ◽  
pp. 852-853
Author(s):  
K. H. Downing ◽  
S. G. Wolf ◽  
E. Nogales
Keyword(s):  
High Resolution ◽  
Structural Data ◽  
Therapeutic Drug ◽  
Zinc Ions ◽  
Two Dimensional ◽  
X Ray Diffraction ◽  
X Ray ◽  
Negative Stain ◽  
Functional Mechanisms ◽  
Tubulin Structure

Microtubules are involved in a host of critical cell activities, many of which involve transport of organelles through the cell. Different sets of microtubules appear to form during the cell cycle for different functions. Knowledge of the structure of tubulin will be necessary in order to understand the various functional mechanisms of microtubule assemble, disassembly, and interaction with other molecules, but tubulin has so far resisted crystallization for x-ray diffraction studies. Fortuitously, in the presence of zinc ions, tubulin also forms two-dimensional, crystalline sheets that are ideally suited for study by electron microscopy. We have refined procedures for forming the sheets and preparing them for EM, and have been able to obtain high-resolution structural data that sheds light on the formation and stabilization of microtubules, and even the interaction with a therapeutic drug.Tubulin sheets had been extensively studied in negative stain, demonstrating that the same protofilament structure was formed in the sheets and microtubules. For high resolution studies, we have found that the sheets embedded in either glucose or tannin diffract to around 3 Å.

A Simple Real-Space Channelling Theory for Electron Diffraction and HREM

1990 ◽  
Vol 48 (1) ◽  
pp. 64-65
Author(s):  
D. Van Dyck
Keyword(s):  
High Resolution ◽  
Electron Diffraction ◽  
Direct Method ◽  
Real Space ◽  
Zone Axis ◽  
Phase Object ◽  
High Resolution Images ◽  
The Many ◽  
Analytical Expressions ◽  
Electron Wavefunction

The computation of the many beam dynamical electron diffraction amplitudes or high resolution images can only be done numerically by using rather sophisticated computer programs so that the physical insight in the diffraction progress is often lost. Furthermore, it is not likely that in this way the inverse problem can be solved exactly, i.e. to reconstruct the structure of the object from the knowledge of the wavefunction at its exit face, as is needed for a direct method [1]. For this purpose, analytical expressions for the electron wavefunction in real or reciprocal space are much more useful. However, the analytical expressions available at present are relatively poor approximations of the dynamical scattering which are only valid either for thin objects ((weak) phase object approximation, thick phase object approximation, kinematical theory) or when the number of beams is very limited (2 or 3). Both requirements are usually invalid for HREM of crystals. There is a need for an analytical expression of the dynamical electron wavefunction which applies for many beam diffraction in thicker crystals. It is well known that, when a crystal is viewed along a zone axis, i.e. parallel to the atom columns, the high resolution images often show a one-to-one correspondence with the configuration of columns provided the distance between the columns is large enough and the resolution of the instrument is sufficient. This is for instance the case in ordered alloys with a column structure [2,3]. From this, it can be suggested that, for a crystal viewed along a zone axis with sufficient separation between the columns, the wave function at the exit face does mainly depend on the projected structure, i.e. on the type of atom columns. Hence, the classical picture of electrons traversing the crystal as plane-like waves in the directions of the Bragg beams which historically stems from the X-ray diffraction picture, is in fact misleading.

High resolution two-dimensional motility mapping; Methodology and initial results

2001 ◽  
Vol 120 (5) ◽  
pp. A226-A226 ◽  
Author(s):  
W LAMMERS ◽  
S DHANASEKARAN ◽  
J SLACK ◽  
B STEPHEN
Keyword(s):  
High Resolution ◽  
Two Dimensional ◽  
Initial Results
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