A film recording and image processing system for confocal microscopy

1990 ◽  
Vol 48 (3) ◽  
pp. 798-799
Author(s):  
Larry D. Ackerman ◽  
W. T. Jansen
Keyword(s):  
Image Processing ◽  
Confocal Microscopy ◽  
Imaging System ◽  
Processing System ◽  
Confocal Imaging ◽  
Hard Copy ◽  
Commercial Development ◽  
Scanning Confocal Microscopy ◽  
Film Recorder ◽  
Digital Film

Scanning confocal microscopy has developed into a very useful technique for many scientific investigations. However, commercial development has been so rapid that some recent advances in computer graphics and imaging have not been incorporated into the commercial systems. One particular concern was high quality hard copy with alpha-numeric and graphic overlays. A subsystem was developed to provide this output for the BioRad MRC-500/600 confocal imaging system.A digital film recorder, an Agfa Matrix Procolor was chosen as the principal element of hardware. This compact unit can record an image at a resolution of 4096 horizontal by 3072 vertical pixels at a cost equivalent to popular analog video film recorders. The interface is a standard IEEE 488 GPIB board. It is compatible with various film emulsions such as Kodak Ecktachrome 100 as well as many of the major graphics arts and image processing programs. The second element of hardware in this system is an ATVista 4M image processing board.

Image Processing for Electron Microscope Investigations of Materials

1983 ◽  
Vol 31 ◽  
Author(s):  
William Krakow
Keyword(s):  
Image Processing ◽  
Text Processing ◽  
Computer Control ◽  
Full Range ◽  
Computer Software ◽  
Processing System ◽  
Image Motion ◽  
Hard Copy ◽  
Digital Television ◽  
Main Component

ABSTRACTA time shared television digital image processing system has been developed for on-line electron microscopy and uses a large mainframe computer. The main component of the system is a digital television frame store which has many standard features for digital analysis such as: digitization, zoom and pan, arithmetic and Boolean processors, alphanumeric generators and so on. Images can be acquired at atomic resolution from a TEM, analyzed in real time and hard copy slides made under full computer control. A full range of computer software has been developed or modified from existing software and is generally compatible with IBM Fortran compilers. Some of the areas where extensive menu driven software has been developed are: particle size and feature analysis, algebraic and geometric image manipulations, Fourier analysis, digitization and process control, image contrast correction, text processing, etc. A number of applications areas have been explored which include: the structure of Si/SiO2interfaces; nucleation of Au on rocksalt; the formation of hexatic structures from amorphous phases under shear, tension and compression; analysis of atomic surface structure and image motion and the analysis of field ion micrographs of amorphous structures. Several of these areas will be discussed in the context of image processing and materials characterization.

Research on the Multi-Spectrum Imaging System Based on Vision Technology

2012 ◽  
Vol 443-444 ◽  
pp. 488-494
Author(s):  
Xuan Hong Jin ◽  
Zheng Yang Zhou ◽  
Ran Xu
Keyword(s):  
Image Processing ◽  
Virtual Instrument ◽  
Imaging System ◽  
Color Image ◽  
Processing System ◽  
New Type ◽  
Spectrum Imaging ◽  
Processing Software ◽  
Image Processing Software ◽  
Labview Platform

This paper introduces an acquiring and processing system of a new type of optical spectrometer based on vision technology. It mainly introduces the hardware structure to acquire the spectrums dispersed by the spectrometer, and the multi-spectrum image processing software as well. Of the different spectrum wavelengths ranges from 400nm to 740nm, the system can create both the color image and the 68 channels gray scale image. Virtual instruments technology is introduced into this system and it makes programming easier and faster by combining virtual instrument and vision technology. The programming of the image processing software uses LabVIEW platform.

Implementation of an automated system for microscope pathological section image with RFID management

2014 ◽  
Vol 31 (8) ◽  
pp. 1691-1708
Author(s):  
Pei-Jarn Chen ◽  
Chia-Hong Yeng ◽  
Ma-Mi Lu ◽  
Sheng-Hsien Chen
Keyword(s):  
Image Processing ◽  
Radio Frequency Identification ◽  
Imaging System ◽  
Processing System ◽  
Database System ◽  
Automated System ◽  
Fluorescence Staining ◽  
Microscopy Imaging ◽  
Microscopic Imaging ◽  
Content Type

Purpose – The purpose of this paper is to establish an automated microscopic imaging database system using a set of Radio Frequency Identification (RFID) management functions to provide a secure storage for hispathology images. Design/methodology/approach – The automated microscopy imaging system is composed mainly of four parts, which include: first, tissue biopsy image acquisition system, second, image processing system, third, RFID system, and fourth, SQL database system. The system has two modes of operation to store and manage hispathology images. First, the hispathology slide undergoes fluorescence staining before acquiring images directly from an external CCD camera connected to the system. Second, the hispathogical slides that have undergone fluorescence staining undergo another microscopic imaging system, and the contents are extracted into a digitized image archive and imported to the system. Also, the system not only acquires images but also performs functions such as displacement correction, image superimposition, and calculation of the total number of fluorescence points. The two methods mentioned above produce the hispathology image files and are tagged using an RFID string index to establish and manage the database system. Findings – The results demonstrated that in the impurities were effectively eliminated in the red fluorescence staining after binarization processing. However, the blue ones remained the same and to solve this problem an adjustable threshold allows users to select the appropriate threshold. Using an additional eigenvalue code to the RFID string provides better encryption mechanism for the patient files and any attempt to tamper the file can easily be detected through the comparison of the eigenvalues. Originality/value – This paper proposes a novel method to implement a more comprehensive, safe, fast, and automated management system for hispathological images using RFID management and image processing techniques. Additional security is provided by including eigenvalues as encryption mechanisms in the Tag string of the RFID.

Spectral imaging system on laser scanning confocal microscopy

2014 ◽  
Vol 22 (6) ◽  
pp. 1446-1453 ◽  
Author(s):  
张运海 ZHANG Yun-hai ◽  
杨皓旻 YANG Hao-min ◽  
孔晨晖 KONG Chen-hui
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Imaging System ◽  
Spectral Imaging ◽  
Laser Scanning Confocal Microscopy ◽  
Scanning Confocal Microscopy

Digital image processing methods applied to the study of annealing time on semiconductor-metal eutectic composites

1988 ◽  
Vol 46 ◽  
pp. 848-849
Author(s):  
J. Hefter
Keyword(s):  
Image Processing ◽  
Digital Image Processing ◽  
Digital Image ◽  
Electronic Device ◽  
Processing System ◽  
Average Diameter ◽  
Eutectic Solidification ◽  
Metal Silicide ◽  
The Matrix ◽  
Microscopic Studies

Semiconductor-metal composites, formed by the eutectic solidification of silicon and a metal silicide have been under investigation for some time for a number of electronic device applications. This composite system is comprised of a silicon matrix containing extended metal-silicide rod-shaped structures aligned in parallel throughout the material. The average diameter of such a rod in a typical system is about 1 μm. Thus, characterization of the rod morphology by electron microscope methods is necessitated.The types of morphometric information that may be obtained from such microscopic studies coupled with image processing are (i) the area fraction of rods in the matrix, (ii) the average rod diameter, (iii) an average circularity (roundness), and (iv) the number density (Nd;rods/cm2). To acquire electron images of these materials, a digital image processing system (Tracor Northern 5500/5600) attached to a JEOL JXA-840 analytical SEM has been used.

Software pattern recognition applied to nanodiffraction patterns from a STEM instrument

1986 ◽  
Vol 44 ◽  
pp. 694-695
Author(s):  
G.Y. Fan ◽  
J.M. Cowley
Keyword(s):  
Image Processing ◽  
Pattern Recognition ◽  
Computer Software ◽  
Processing System ◽  
Light Level ◽  
Host Computer ◽  
Low Light ◽  
Image Processing System ◽  
Recent Developments ◽  
On Line

In recent developments, the ASU HB5 has been modified so that the timing, positioning, and scanning of the finely focused electron probe can be entirely controlled by a host computer. This made the asynchronized handshake possible between the HB5 STEM and the image processing system which consists of host computer (PDP 11/34), DeAnza image processor (IP 5000) which is interfaced with a low-light level TV camera, array processor (AP 400) and various peripheral devices. This greatly facilitates the pattern recognition technique initiated by Monosmith and Cowley. Software called NANHB5 is under development which, instead of employing a set of photo-diodes to detect strong spots on a TV screen, uses various software techniques including on-line fast Fourier transform (FFT) to recognize patterns of greater complexity, taking advantage of the sophistication of our image processing system and the flexibility of computer software.

EMCAT: An automatic image-processing system for electron-microscopic tomography

1994 ◽  
Vol 52 ◽  
pp. 418-419
Author(s):  
Weiping Liu ◽  
John W. Sedat ◽  
David A. Agard
Keyword(s):  
Image Processing ◽  
Structural Information ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Processing System ◽  
Electron Microscopic ◽  
Data Set ◽  
Ordered Structures ◽  
Automatic Image Processing ◽  
Em Tomography

Any real world object is three-dimensional. The principle of tomography, which reconstructs the 3-D structure of an object from its 2-D projections of different view angles has found application in many disciplines. Electron Microscopic (EM) tomography on non-ordered structures (e.g., subcellular structures in biology and non-crystalline structures in material science) has been exercised sporadically in the last twenty years or so. As vital as is the 3-D structural information and with no existing alternative 3-D imaging technique to compete in its high resolution range, the technique to date remains the kingdom of a brave few. Its tedious tasks have been preventing it from being a routine tool. One keyword in promoting its popularity is automation: The data collection has been automated in our lab, which can routinely yield a data set of over 100 projections in the matter of a few hours. Now the image processing part is also automated. Such automations finish the job easier, faster and better.

Laser Scanning Confocal Microscopy and Three-Dimesnsional Reconstruction of the Mitotic Spindles of Unequally Dividing Embryonic Cells

1990 ◽  
Vol 48 (3) ◽  
pp. 166-167
Author(s):  
J. Holy ◽  
G. Schatten
Keyword(s):  
Confocal Microscopy ◽  
Mitotic Spindle ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
Two Dimensions ◽  
Embryonic Cells ◽  
Mitotic Spindles ◽  
Cleavage Division ◽  
Scanning Confocal Microscopy

One of the classic limitations of light microscopy has been the fact that three dimensional biological events could only be visualized in two dimensions. Recently, this shortcoming has been overcome by combining the technologies of laser scanning confocal microscopy (LSCM) and computer processing of microscopical data by volume rendering methods. We have employed these techniques to examine morphogenetic events characterizing early development of sea urchin embryos. Specifically, the fourth cleavage division was examined because it is at this point that the first morphological signs of cell differentiation appear, manifested in the production of macromeres and micromeres by unequally dividing vegetal blastomeres.The mitotic spindle within vegetal blastomeres undergoing unequal cleavage are highly polarized and develop specialized, flattened asters toward the micromere pole. In order to reconstruct the three-dimensional features of these spindles, both isolated spindles and intact, extracted embryos were fluorescently labeled with antibodies directed against either centrosomes or tubulin.

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