scholarly journals The use of sorbitol-MacConkey agar in conjunction with a specific antiserum for the detection of Vero cytotoxin-producing strains ofEscherichia coliO 157

1988 ◽  
Vol 101 (2) ◽  
pp. 327-335 ◽  
Author(s):  
H. Kleanthous ◽  
N. K. Fry ◽  
H. R. Smith ◽  
R. J. Gross ◽  
B. Rowe
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
Screening Method ◽  
Bloody Diarrhoea ◽  
Macconkey Agar ◽  
Haemorrhagic Colitis ◽  
Faecal Samples ◽  
Genes Encoding ◽  
Homologous Antiserum ◽  
O 157

SUMMARYUsing DNA probes specific for the genes encoding Vero cytotoxins 1 and 2 in hybridization experiments on faecal samples. Vero cytotoxin-producingEscherichia coli(VTEC) of serogroup 0 157 were detected in 21 of 63 cases of haemorrhagic colitis, 9 of 31 cases of non-bloody diarrhoea and 14 of 68 cases of haemolytic uraemic syndrome. Compared with these results sorbitol-MacConkey agar in conjunction with a specific 0 157 antiserum gave a sensitivity of 62% in haemorrhagic colitis, 56% in non-bloody diarrhoea and 57% in haemolytic uraemic syndrome.The specificity of this method was 100% in all three groups. This demonstrates that sorbitol-MacConkey agar is a useful screening method for the detection of VTEC of serogroup O 157 when used in conjunction with a specific homologous antiserum. However, this method does not detect VTEC belonging to other serogroups and such strains were found, particularly in cases of haemolytic uraemic syndrome.

scholarly journals Verotoxin-producingEscherichia coliinfections in Sheffield: cattle as a possible source

1989 ◽  
Vol 102 (3) ◽  
pp. 439-445 ◽  
Author(s):  
P. A. Chapman ◽  
D. J. Wright ◽  
P. Norman
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
Matched Control ◽  
Bloody Diarrhoea ◽  
E Coli ◽  
Faecal Samples ◽  
O 157 ◽  
Age And Sex

SUMMARYDuring 1986 and 1987, faecal samples from patients with haemorrhagie colitis (HC) or haemolytic-uraemic syndrome (HUS) were examined for evidence of infection by verotoxin-producingEscherichia coli(VTEC). During the 2-year period VTEC infections were found in 31 (78%) of 40 patients initially presenting with HC, and in 5 (63%) of 8 patients initially presenting with HUS, VTEC were found in only 2 (0·9%) of 229 age and sex matched control patients with acute non-bloody diarrhoea. All but one VTEC belonged toE. coliserogroup O 157. During 1987 this serogroup was isolated from 2 (1%) of 207 samples of faeces taken from cattle arriving at a Sheffield abattoir, indicating a possible source of these infections for man. We are unaware of previous reports of isolation of this organism from cattle in England.

scholarly journals A christening party outbreak of haemorrhagic colitis and haemolytic uraemic syndrome associated with Escherichia coli O 157. H7

1989 ◽  
Vol 103 (2) ◽  
pp. 249-254 ◽  
Author(s):  
R. L. Salmon ◽  
I. D. Farrell ◽  
J. G. P. Hutchison ◽  
D. J. Coleman ◽  
R. J. Gross ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Point Source ◽  
Haemolytic Uraemic Syndrome ◽  
E Coli ◽  
Haemorrhagic Colitis ◽  
Source Of Infection ◽  
O 157

SUMMARYA point source outbreak of haemorrhagic colitis due to Escherichia coli O 157. H7 producing verocytotoxin (VT), took place following a christening party in Birmingham in June 1987. Twenty-six people were affected, six were admitted to hospital and one developed haemolytic uraemic syndrome: there were no deaths. VT + E. coli O 157. H7 was isolated from 13 (57%) of 23 faecal specimens from affected people and from 3 (9%) of 33 specimens from asymptomatic people. Free VT was detected in the faeces of one further asymptomatic person. Illness was associated with eating turkey-roll sandwiches (P < 0·001) suggesting that cold meats might be an important source of infection.

scholarly journals Sorbitol-fermenting Shiga toxin-producing Escherichia coli O157: indications for an animal reservoir

2005 ◽  
Vol 134 (4) ◽  
pp. 719-723 ◽  
Author(s):  
D. ORTH ◽  
K. GRIF ◽  
M. P. DIERICH ◽  
R. WÜRZNER
Keyword(s):  
Escherichia Coli ◽  
Clinical Diagnosis ◽  
Shiga Toxin ◽  
Haemolytic Uraemic Syndrome ◽  
Stool Specimen ◽  
Farm Animals ◽  
Molecular Characteristics ◽  
Escherichia Coli O157 ◽  
Animal Reservoir ◽  
Faecal Samples

This study investigates a sorbitol-fermenting enterohaemorrhagic Escherichia coli (SF EHEC) O157 infection in a farmer's family in the Austrian province of Salzburg. The investigation commenced after a 10-month-old boy was admitted to hospital with the clinical diagnosis of a haemolytic–uraemic syndrome (HUS) and his stool specimen grew SF EHEC O157:H−. In a subsequent environmental survey, a stool specimen of the 2-year-old brother and faecal samples of two cattle from the family's farm were also found to be positive for SF EHEC O157:H−. All four isolates had indistinguishable phenotypic and molecular characteristics and were identical to the first strain detected in Bavaria in 1988. Despite identical isolates being demonstrated in Bavaria after 1988, and until this report, increased surveillance in neighbouring Austria had not found this organism. We propose that the strain may have recently spread from Bavaria to Austria. Although SF EHEC O157:H− strains are still rare, they may represent a considerable health threat as they can spread from farm animals to humans and between humans.

scholarly journals An outbreak of infection due to verocytotoxin-producing Escherichia coli O157 in four families: the influence of laboratory methods on the outcome of the investigation

1997 ◽  
Vol 119 (2) ◽  
pp. 113-119 ◽  
Author(s):  
P. A. CHAPMAN ◽  
C. A. SIDDONS ◽  
J. MANNING ◽  
C. CHEETHAM
Keyword(s):  
Immunomagnetic Separation ◽  
Bloody Diarrhoea ◽  
Farm Animals ◽  
Secretory Iga ◽  
Macconkey Agar ◽  
E Coli ◽  
Faecal Samples ◽  
The Family ◽  
Iga Response

Three members of family A, who had diarrhoea on 20 October, lived on a small arable farm which had 10 cattle. Manure from the animals was used to fertilize the ground for growing potatoes which were then offered for retail sale, unwashed, directly from the farm. The mother from family B bought potatoes, which were covered with manure, from family A in early November and over the subsequent 10 days she became ill with diarrhoea and her daughter and son both became ill with bloody diarrhoea. The mother from family C visited family B while the daughter from the latter family was symptomatic; the mother developed diarrhoea several days later. The mother and two sons from family D visited family B while the son from the latter family was symptomatic; the first son developed bloody diarrhoea 6 days later which progressed to development of haemolytic-uraemic syndrome. Direct culture of faecal samples onto cefixime rhamnose sorbitol MacConkey agar failed to isolate E. coli O157 from any of the symptomatic patients, and direct culture onto cefixime tellurite sorbitol MacConkey agar isolated the organism from only one patient. In contrast, a combination of isolation of E. coli O157 by immunomagnetic separation and detection of E. coli O157-specific secretory IgA, suggested E. coli O157 infection in all eight symptomatic patients, but not in any of the family members who were not ill. Two children who excreted the organism for 60 and 89 days respectively were the only two patients who did not develop a secretory IgA response. E. coli O157 was not isolated from potatoes from the farm and faecal samples from the farm animals were not available for examination. The study illustrates the need to use the most sensitive methods available during the investigation and follow up of cases of E. coli O157 infection.

scholarly journals First recognized community outbreak of haemorrhagic colitis due to verotoxin-producingEscherichia coliO 157.H7 in the UK

1988 ◽  
Vol 101 (1) ◽  
pp. 83-91 ◽  
Author(s):  
G. M. Morgan ◽  
C. Newman ◽  
S. R. Palmer ◽  
J. B. Allen ◽  
W. Shepherd ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Case Control Study ◽  
Case Control ◽  
Adult Women ◽  
East Anglia ◽  
Haemorrhagic Colitis ◽  
The United Kingdom ◽  
O 157 ◽  
The Uk ◽  
Control Study

SUMMARYThe first recognized outbreak of haemorrhagic colitis due toEscherichia coliO 157. H7 in the United Kingdom affected at least 24 persons living in East Anglia over a 2-week period. Theillnesses were characterized by severe abdominal pain and bloody diarrhoea of short duration. Eleven patients were admitted to hospital and there was one death. Patients were mainly adult women who had not eaten out of the home in the 2 weeks before onset. Unlike previously reported outbreaks hamburgers were not the vehicle of infection, and a case-control study suggested that handling vegetables, and particularly potatoes, was the important risk factor.

scholarly journals Detection of auto-transporter Sat and Vat genes among Escherichia coli strains isolated from urinary tract infections

2019 ◽  
Vol 23 (10) ◽  
pp. 40 ◽  
Author(s):  
Wisal R. Yaseen AL- Hayali1 ◽  
Alaa Younis Mahdy2 ◽  
Muhammad Abdul Zaraq Ibrahim3
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Duplex Pcr ◽  
Macconkey Agar ◽  
Biochemical Tests ◽  
E Coli ◽  
Genes Encoding ◽  
Tract Infections ◽  
Pcr Techniques

This study was designed to detect the presence of genes encoding autotranspoter proteins in E. coli that causes UTI by using PCR techniques. Seventy two urine sample were collected from patients infected with UTI whom attended to Salah-AL-deen general hospital in Tikrit city, during three months period (September to November 2016). All samples were cultivated on Blood agar and MacConkey agar. The 47(65.2%) E. coli isolates were confirmed using standard biochemical tests for E. coli. The results indicate the frequencies of Sat gene was 27 strains(57.5%) while Vat gene was 12 strains (25.5%) while the Duplex PCR detected 8(17%) strains of E. coli contained two genes. With this method, we confirmed that autotransporter genes are pathospecifically distributed among the E. coli strains studied.   http://dx.doi.org/10.25130/tjps.23.2018.167

scholarly journals Outbreak of haemolytic uraemic syndrome and bloody diarrhoea due to Escherichia coli O104:H4, south-west France, June 2011

2011 ◽  
Vol 16 (26) ◽  
Author(s):  
G Gault ◽  
F X Weill ◽  
P Mariani-Kurkdjian ◽  
N Jourdan-da Silva ◽  
L King ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
Bloody Diarrhoea ◽  
Binary File ◽  
South West

Binary file ES_Abstracts_Final_ECDC.txt matches

scholarly journals Properties of strains ofEscherichia colibelonging to serogroup O 157 with special reference to production of Vero cytotoxins VTl and VT2

1987 ◽  
Vol 99 (3) ◽  
pp. 613-624 ◽  
Author(s):  
S. M. Scotland ◽  
G. A. Willshaw ◽  
H. R. Smith ◽  
B. Rowe
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Antimicrobial Agent ◽  
Special Reference ◽  
Haemolytic Uraemic Syndrome ◽  
Antimicrobial Agents ◽  
Human Origin ◽  
Plasmid Content ◽  
O 157 ◽  
Porcine Origin

SUMMARYFifty-four strains ofEscherichia colibelonging to serogroup O 157 were examined for the production of Vero cytotoxins VT1 and VT2, and for other properties such as plasmid content, resistance to antimicrobial agents and colicin production. Twenty-six strains from cases of diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome in humans produced VT. By serum neutralization tests and hybridization with DNA probes for VTl or VT2, three classes were recognized which produced either VTl alone or VT2 alone or both VTl and VT2. These strains were of H type 7 or non-motile. The strains producing VT were sensitive to all the antimicrobial agents tested, and all carried at least one plasmid which had a molecular weight ofc.60 × 106. Seven strains of porcine origin and 21 strains of human origin did not produce VT or hybridize with either DNA probe. None of these strains was of H type 7. Of the 21 human VT” strains, 17 were of extraintestinal origin and 18 were of H type 45. Twenty-three of the 28 VT” strains were resistant to at least one antimicrobial agent.

scholarly journals Optimizing a Screening Protocol for Potential Extended-Spectrum β-Lactamase Escherichia coli on MacConkey Agar for Use in a Global Surveillance Program

2020 ◽  
Vol 58 (9) ◽  
Author(s):  
Megan E. Jacob ◽  
Shivaramu Keelara ◽  
Awa Aidara-Kane ◽  
Jorge R. Matheu Alvarez ◽  
Paula J. Fedorka-Cray
Keyword(s):  
Escherichia Coli ◽  
Screening Method ◽  
Cost Effective ◽  
Surveillance Program ◽  
Macconkey Agar ◽  
Middle Income ◽  
Content Type ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

ABSTRACT The increasing prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is worrisome. Coordinated efforts to better understand global prevalence and risk factors are needed. Developing lower- and middle-income countries need reliable, readily available, and cost-effective solutions for detecting ESBL E. coli to contribute to global surveillance. We evaluated MacConkey agar supplemented with ceftriaxone or cefotaxime as a screening method for accurately detecting and quantifying potential ESBL E. coli. MacConkey agar from eight manufacturers, representing seven countries, was prepared with 2 or 4 μg/ml ceftriaxone or cefotaxime. Four E. coli strains (NC11, ATCC 25922, CM-13457, and CM-10455) and one Klebsiella pneumoniae strain (CM-11073) were grown overnight, serially diluted, and plated in triplicate for enumeration on all medium combinations. After recovery was assessed, US-1 MacConkey agar with cefotaxime was used to further evaluate the reproducibility and detection of potential ESBL E. coli from poultry cecal (n = 30) and water (n = 30) samples. Results indicated the recovery of E. coli 13457 from four MacConkey agar manufacturers was reduced by up to 4 log CFU/ml, and phenotypic differences in colony size and color were apparent for each manufacturer for control E. coli strains. A true ESBL, NC11, was not reduced with 4 μg/ml cefotaxime. From ceca and water, potential ESBL E. coli isolates were only confirmed from MacConkey agar with 4 μg/ml cefotaxime, where 45% and 16.6% of E. coli isolates phenotypically expressed ESBL production. The quality and reproducibility of MacConkey agar varied by manufacturer, which suggests that a single manufacturer and medium type should be selected for global monitoring efforts so that training and interpretation can be standardized.

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