Relationship between the level of antibodies in bulk tank milk and the within-herd seroprevalence of Coxiella burnetii in cows

2011 ◽  
Vol 140 (9) ◽  
pp. 1710-1713 ◽  
Author(s):  
A.-F. TAUREL ◽  
R. GUATTEO ◽  
A. JOLY ◽  
F. BEAUDEAU
Keyword(s):  
Phase I ◽  
Dairy Cows ◽  
Coxiella Burnetii ◽  
Present Finding ◽  
General Linear Model ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bulk Tank Milk ◽  
Bulk Tank ◽  
Antibody Levels ◽  
The Relationship

SUMMARYThe relationship between the level of antibodies in bulk tank milk (BTM) and the within-herd seroprevalence of Coxiella burnetii in cows was assessed. Blood from milking cows and BTM were sampled in 55 infected herds and tested using commercial enzyme-linked immunosorbent assay (ELISA) kits. The relationship between antibody levels and WHP, which was quantified using a general linear model, was only moderate (R2=0·15). Nevertheless, the lowest antibody level in BTM was associated with the lowest mean within-herd prevalence. The present finding indicates that ELISA applied to BTM could identify infected herds with quite low within-herd seroprevalence. For such herds, the vaccination of dairy cows as well as nulliparous heifers using a phase I vaccine could effectively prevent C. burnetii shedding.

scholarly journals Serological screening for Coxiella burnetii in the context of early pregnancy loss in dairy cows

2020 ◽  
Author(s):  
Attila Dobos ◽  
György Gábor ◽  
Enikő Wehmann ◽  
Béla Dénes ◽  
Bettina Póth-Szebenyi ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Coxiella Burnetii ◽  
Pregnancy Loss ◽  
Q Fever ◽  
Early Stage ◽  
Dairy Farms ◽  
Serological Screening ◽  
Bulk Tank Milk ◽  
Seropositivity Rate ◽  
Bulk Tank

AbstractQ fever is one of the commonest infectious diseases worldwide. A Coxiella burnetii prevalence of 97.6% has been found by ELISA and PCR tests of the bulk tank milk in dairy cattle farms of Hungary. The herd- and individual-level seroprevalence rates of C. burnetii in the examined dairy cows and farms have dramatically increased over the past ten years. Three high-producing industrial dairy farms were studied which had previously been found ELISA and PCR positive for C. burnetii by bulk tank milk testing. Coxiella burnetii was detected in 52% of the 321 cows tested by ELISA. Pregnancy loss was detected in 18% of the cows between days 29–35 and days 60–70 of gestation. The study found a higher seropositivity rate (80.5%) in the cows that had lost their pregnancy and a seropositivity of 94.4% in the first-bred cows that had lost their pregnancy at an early stage. The ELISA-positive pregnant and aborted cows were further investigated by the complement fixation test (CFT). In dairy herds an average of 66.6% individual seropositivity was detected by the CFT (Phase II) in previously ELISA-positive animals that had lost their pregnancy and 64.5% in the pregnant animals. A higher (Phase I) seropositivity rate (50.0%) was found in the cows with pregnancy loss than in the pregnant animals (38.5%). The high prevalence of C. burnetii in dairy farms is a major risk factor related to pregnancy loss.

Measuring antibody levels in bulk-tank milk as an epidemiological tool to search for the status of Coxiella burnetii in dairy sheep

2011 ◽  
Vol 139 (10) ◽  
pp. 1631-1636 ◽  
Author(s):  
F. RUIZ-FONS ◽  
I. ASTOBIZA ◽  
J. F. BARANDIKA ◽  
R. A. JUSTE ◽  
A. HURTADO ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Population Level ◽  
Cost Effective ◽  
Epidemiological Surveillance ◽  
Dairy Sheep ◽  
Bulk Tank Milk ◽  
Epidemiological Tool ◽  
The Status ◽  
Bulk Tank ◽  
Antibody Levels

SUMMARYPooling samples may provide a valuable alternative to individual testing for pathogen surveillance purposes. We studied the reliability of measuring the level of antibodies against Coxiella burnetii in bulk-tank milk (BTM) to estimate the seroprevalence of C. burnetii in dairy sheep in 34 flocks. We then estimated the seroprevalence of C. burnetii in 154 dairy sheep flocks according to the level of antibodies in BTM. We tested for the accuracy of our estimation at the population level by comparing predicted mean C. burnetii flock seroprevalence with that obtained in another survey performed on the same population. Our findings showed that testing BTM by ELISA is a cost-effective and relatively good index of the seroprevalence of C. burnetii in dairy sheep and may be a useful tool for epidemiological surveillance at the population level.

scholarly journals Molecular prevalence of Coxiella burnetii in bulk-tank milk from bovine dairy herds: Systematic review and meta-analysis

One Health ◽  
2020 ◽  
pp. 100208
Author(s):  
Ana Rabaza ◽  
Martín Fraga ◽  
Luis Gustavo Corbellini ◽  
Katy M.E. Turner ◽  
Franklin Riet-Correa ◽  
...  
Keyword(s):  
Systematic Review ◽  
Coxiella Burnetii ◽  
Meta Analysis ◽  
Dairy Herds ◽  
Bulk Tank Milk ◽  
Bulk Tank

scholarly journals Mycoplasmal mastitis in dairy cows in the Moghan region of Ardabil State, Iran : short communication

2006 ◽  
Vol 77 (4) ◽  
Author(s):  
C. Ghazaei
Keyword(s):  
Dairy Cows ◽  
Short Communication ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Mycoplasma Bovis ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
The World ◽  
Bulk Tank

Mycoplasmas are an important and economically significant cause of mastitis in dairy cows in various parts of the world. The organisms are highly contagious, with the main reservoir of infection originating from cows with subclinical mastitis. In 1998 the 1st cases of bovine mastitis due to Mycoplasma bovis were diagnosed in Ardabil State, Iran. An investigation was carried out with the aim of establishing the extent of mycoplasma infections in dairy cows in Ardabil State. Milk samples obtained from 80 cows with clinical mastitis were cultured in the laboratory for the presence of mycoplasmas. Similarly, 48 bulk-tank milk samples were examined for the presence of mycoplasmas. A modified Hayflick broth was used to isolate the mycoplasmas and an immunoperoxidase test used for the species identification of the isolates. Mycoplasma bovis was isolated from 39 (48.75 %) of the clinical mastitis samples and from 48 of the bulk-tank milk samples tested. This indicated that mycoplasma udder infections were more prevalent in dairy cows in Ardabil State than previously thought.

scholarly journals Association between antibodies to Coxiella burnetii in bulk tank milk and perinatal mortality of Danish dairy calves

2011 ◽  
Vol 53 (1) ◽  
Author(s):  
Katrine T Nielsen ◽  
Søren S Nielsen ◽  
Jens F Agger ◽  
Anna-Bodil Christoffersen ◽  
Jørgen S Agerholm
Keyword(s):  
Perinatal Mortality ◽  
Coxiella Burnetii ◽  
Dairy Calves ◽  
Bulk Tank Milk ◽  
Bulk Tank ◽  
Danish Dairy

scholarly journals Serological and shedding patterns after Coxiella burnetii vaccination in the third gestation trimester in dairy cows

2014 ◽  
Vol 62 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Joan Tutusaus ◽  
Fernando López-Gatius ◽  
Beatriz Serrano ◽  
Eva Monleón ◽  
Juan Badiola ◽  
...  
Keyword(s):  
Phase I ◽  
Dairy Cows ◽  
Coxiella Burnetii ◽  
Post Partum ◽  
Antibody Detection ◽  
Vaginal Fluid ◽  
Third Trimester ◽  
The Third ◽  
Kaplan Meier ◽  
Highly Correlated

This study sought to assess the effects of an inactivated phase I vaccine against Coxiella burnetii at the start of the third trimester of gestation on serological profiles, bacterial shedding patterns and subsequent reproductive performance in dairy cows. Cows were randomly assigned to a control (n = 78) or a vaccinated (n = 78) group on days 171–177 of gestation. Samples of placenta and colostrums at parturition, vaginal fluid, faeces, milk (PCR identification) and blood (anti-C. burnetii antibody detection) were obtained on the day of treatment and on days 91–97 post partum, and also on parturition day and weekly on days 1–7, 8–14, 15–21, 22–28 and 29–35 post partum in a subset of 70 animals. By Kaplan-Meier survival analysis, no significant effect of vaccination was detected on any of the reproductive variables studied. According to the odds ratio, C. burnetii shedding on days 171–177 of gestation was highly correlated with seropositivity against C. burnetii (OR = 9.1), while vaccination was not linked to reduced shedding of the bacterium. In shedders compared to others, the likelihood of pregnancy to first AI decreased and increased by factors of 0.26 and 16.1 on days 1–35 and 91–97 post partum, respectively. In conclusion, when administered at the start of the third trimester of pregnancy, the inactivated C. burnetii phase I vaccine failed to reduce bacterial shedding.

Prevalence of Coxiella burnetii infection in Dutch dairy herds based on testing bulk tank milk and individual samples by PCR and ELISA

2011 ◽  
Vol 168 (3) ◽  
pp. 79-79 ◽  
Author(s):  
J. Muskens ◽  
E. van Engelen ◽  
C. van Maanen ◽  
C. Bartels ◽  
T. J. G. M. Lam
Keyword(s):  
Coxiella Burnetii ◽  
Dairy Herds ◽  
Bulk Tank Milk ◽  
Bulk Tank
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