GROWTH AND EARLY REPRODUCTION DEVELOPMENT OF THE FIRST GENERATION OF SHEATFISH, Ompok miostoma (Vaillant, 1902) REARED IN CONTROLLED CONCRETE TANKS

This study was conducted to observe the growth and reproductive biology of wild sheatfish in controlled concrete tanks. The research was carried out from January to November 2020 at the Research Station for Freshwater Fisheries Germplasm, Cijeruk, Bogor. Sampled fish were captured from Cilala Lake in Bogor and Cipanas River in Sumedang, West Java. The collected fish were 14.6 ± 2.24 cm in length and 21.5 ± 9.19 g in weight. Growth and reproductive biology parameters were measured every 30 days. Cortisol, estradiol, vitellogenin, testosterone, glucose, and hemoglobin were also determined as the supporting parameters for gonadal maturity. Measured water quality parameters were temperature, pH, dissolved oxygen, ammonia, and alkalinity. The results showed that sheatfish grew with a specific growth rate of 0.4 ± 0.15%/day, FCR of 3.2 ± 0.26, and survival rate of 100%. Observation on the gonad maturity found that the fish studied were in the level-I and II. There are no significant differences regarding the supporting parameters (P>0.05) on the fish examined, despite the tendency of decreasing cortisol, and increasing testosterone. Based on the results, this study concludes that the observed fish can adapt, grow, and start to mature their gonads in their new environment, with temperature as one of the possible key factors influencing its gonad development.

Evaluation of Probiotic-Fermented Feed Addition and LaserFiring to Accelerate Mature Broodstocks and Seed Productions of African Catfish (Clarias gariepinus)

This study aimed to determine the effects of fermented feed using probiotics and laser-firing to accelerate the mature broodstocks and seed productions of African catfish (Clarias gariepinus). Fish has used male and female broodstocks of catfish. The method was used a completely randomized design with three treatments: unfermented feed as control, probiotic-fermented feed (PFF), and probioticfermented feed+laser firing (PFF+Li). In the first study, a laser-firing dose of 1.125 Joule was performed on fish broodstocks every 15 days. The gonadal maturity time of male and female broodstocks was observed. The second study, treated female broodstocks, was mated with mature male broodstocks without any prior treatments (control). Fertilization rate, hatching rate, and seed production performances such as survival rate and total length were measured. The results showed that treatment of PFF+Li has a significant effect (P<0.05) on the gonadal maturity time of males and females. This treatment reaches the fastest time to mature of the female gonad (31-41 days) and the male gonad (32-37 days) than other treatments (P<0.05). This treatment also produced the highest fertilization, hatching, and survival rates of more than 90%, respectively, and the highest seed yield of 2.1-3.0 cm size compared to other treatments (P<0.05) in African catfish.

EFEK TERAPI HORMON OODEV TERHADAP PERKEMBANGAN TINGKAT KEMATANGAN GONAD IKAN KAKAP MERAH (Lutjanus sp.) DI TELUK TALENGEN

Marine aquaculture activities in the Sangihe Islands, more specifically in the waters of Talengen Bay, are not yet optimal and still utilize seeds from catches in nature. This study aims to determine the effect of using the Oodev hormone and obtain the best dose to stimulate the gonadal maturity level (TKG) of red snapper (Lutjanus sp.). The red snapper used comes from the catch in the waters of Talengen Bay and is also domesticated in floating net cages. The sampled fish used were 60 individuals with average body weight (30.5 g). The Oodev hormone dose treatment, namely; 0 mL/kg, 0.5 mL/kg, 1 mL/kg and 1.5 mL/kg. Oodev hormone was applied to commercial feed specifically for marine fish with the trademark Megami GR-3 orally for 30 days of rearing. The results showed that the administration of the Oodev hormone had a positive effect on the gonadal maturity level of red snapper, with the development of different levels of gonadal maturity in each treatment. The 0.5 mL/kg dose treatment resulted in the highest gonadal maturity level (TKG III). The maturation stage was followed by 1 mL/kg and 1.5 mL/kg (TKG II) treatments. Meanwhile, compared with the 0 mL/kg treatment, the developmental stage was still TKG I (immature). These findings provide Oodev hormone with 0.5 mL/kg dose could stimulate the red snapper gonad maturity level to mature for 30 days of rearing.

Morfometri, Pemijahan, dan Indeks Kematangan Gonad Teripang Komersial di Perairan Pulau Nyamuk, Karimunjawa

Sea cucumbers are marine organisms that live in coral reef ecosystem habitats and their associations. Sea cucumbers have been used by communities as food, cosmetics and traditional medicine in various countries. The benefits and high economic value of sea cucumbers make fisheries activities occured massively and continuously. Sea cucumbers were large commodity from Indonesian waters. However, until now it has not received proportional attention. The lack of information on morphometry, spawning in various regions has made the commodity of sea cucumbers decreased. This research was conducted to determine the character of commercial sea cucumber commodities in Pulau Nyamuk, to the occurrence of spawning based on the moon phase. This research was conducted in September - November 2020. This research method uses an analysis approach of gonadal maturity index in determining the spawning of sea cucumbers. The results showed that commercial sea cucumbers in Pulau Nyamuk were Pisang (Holothuria sp.) and Gamat (Stichopus sp.) Sea cucumber. This sea cucumber weighs 300-690 grams wet. Sea Cucumbers, Pisang and Gamat tend to have high IKG values in the new moon phase with IKG index values >10%. Teripang merupakan organisme laut yang hidup pada habitat ekosistem terumbu karang dan asosiasinya. Teripang telah dimanfaatkan oleh masyarakat di sekitar pantai sebagai bahan makanan, kosmetik serta obat tradisional di berbagai negara. Manfaat serta nilai ekonomi teripang yang tinggi membuat aktivitas pemburuan terjadi secara masif dan terus menerus. Teripang juga merupakan komoditas besar yang berasal dari Perairan Indonesia. Namun hingga saat ini belum memperoleh perhatian yang proporsional. Minimnya informasi mengenai morfometri, pemijahan di berbagai daerah membuat komoditas teripang turun secara drastis. Penelitian ini dilakukan untuk mengetahui karakter komoditas teripang komersial di Pulau Nyamuk, hingga terjadinya pemijahan berdasarkan fase bulan. Penelitian ini bulan September - November 2020 di Perairan Pulau Nyamuk Kecamatan Karimunjawa, Kabupaten Jepara. Metode penelitian ini menggunakan pendekatan analisis indeks kematangan gonad dalam menentukan pemijahan teripang. Hasil penelitian menunjukkan bahwa teripang komersial di Pulau Nyamuk adalah jenis teripang Pisang (Holothuria sp.) dan Gamat (Stichopus sp.). Teripang ini mempunyai berat 300-690 gram basah. Teripang Pisang dan Gamat cenderung mempunyai nilai IKG yang tinggi pada bulan baru dengan nilai indeks IKG >10%.

Stock status of Nile tilapia (Oreochromis niloticus) in Aneuk Laot Lake, Sabang District, Aceh Province, Indonesia

Prianto E, Purwoko RM, Kasim K. 2021. Stock status of Nile tilapia (Oreochromis niloticus) in Aneuk Laot Lake, Sabang District, Aceh Province, Indonesia. Biodiversitas 22: 3364-3370. The Nile tilapia (Oreochromis niloticus) fishery in Aneuk Laot Lake plays a vital role in the local livelihoods. There is an indication of the massive exploitation of the species that potentially harm the stock and threaten its sustainability. This research aims to evaluate and assess the Nile Tilapia's stock status and use the results as baseline information to the management authorities. Surveys were conducted from May 2018 to April 2019 by collecting the length-weight data, sex, and gonadal maturity stage of 600 individuals. The Length-Based Spawning Potential Ratio (LB-SPR) was used to determine the Spawning Potential Ratio (SPR) and compared the results to the reference point. The results show that 50% of the females have matured at 15.1 cm, while the males at 17.8 cm. The individual fish length varies from 12.15 to 20.5 cm, whereas 50% of them were caught at size 17.8 cm (Lc50), meaning that predominantly of the fish had experienced first spawning before they were captured. The current SPR is about 5%, confirming the Nile tilapia's stock status is at an unhealthy state. Consequently, the precautionary approach, such as increasing the mesh size and limiting effort, should be considered to ensure the sustainability of the recruitment.

Effects of Diethylstilbestrol on Zebrafish Gonad Development and Endocrine Disruption Mechanism

Environmental estrogen is a substance that functions as an endocrine hormone in organisms and can cause endocrine system disruption. A typical environmental estrogen, diethylstilbestrol (DES), can affect normal sexual function and organism development. However, even though the effects of different exposure stages of DES on the endocrine system and gonadal development of zebrafish juveniles are unknown, sex determination is strongly influenced by endocrine-disrupting chemicals (EDCs). From 10–90 days post fertilization (dpf), juvenile zebrafish were exposed to DES (100 and 1000 ng/L) in three different stages (initial development stage (IDS), 10–25 dpf; gonadal differentiation stage (GDS), 25–45 dpf and gonadal maturity stage (GMS), 45–60 dpf). Compared with that of IDS and GMS, the growth indicators (body length, body weight, and others) decreased significantly at GDS, and the proportion of zebrafish females exposed to 100 ng/L DES was significantly higher (by 59.65%) than that of the control; in addition, the zebrafish were biased towards female differentiation. The GDS is a critical period for sex differentiation. Our results show that exposure to environmental estrogen during the critical gonadal differentiation period not only affects the development of zebrafish, but also affects the population development.

MATURASI LELE MUTIARA Clarias gariepinus BETINA MELALUI PENAMBAHAN HORMON OODEV DAN ESTRADIOL-17ß PADA PAKAN

The problem in Mutiara strain of North African catfish Clarias gariepinus culture is that the production of both larvae and seeds is still inhibited by the seasonal reproductive cycle where the catfish only spawn once during the spawning season. The purposes of this study were to evaluate the effect of administration of oodev and estradiol-17ß on the maturity process and to determine the best doses in accelerating the maturion of female Mutiara strain. This study used a Completely Randomized Design with 5 treatments and 3 individual replications. The treatments were combination between dose Oodev (PMSG and Anti-Dopamine) and dose Estradiol-17ß in feed (both doses in ml/kg feed):A(0+0), B(0.5+0), C(0+0.6), D(0.5+0,6), E(1+1). The results of this study showed that the administration of Oodev and Estradiol-17ß significant affect on gonadal maturity index about 82.21% compared to control and acclerating maturation of female mutiara strain with doses the best is the treatment E (oodev 1 ml/kg feed+estradiol-17ß 1 ml/kg feed).Key words: Clarias gariepinus, dose, estradiol-17ß, maturation, oodev

A review of recent sightings and reports of the giant tiger shrimp Penaeus monodon (Decapoda: Penaeidae) on the Mexican coast of the Gulf of Mexico (2012-2019)

This work shows an update of the sightings and reports of tiger shrimp on the Mexican coast of the Gulf of Mexico recorded from 2012 to 2019 and reports the smallest specimen found in the western Atlantic. The results show an increase of almost 300% in sightings and reports since the first report. The zone with most sightings and reports was the coast of Campeche and Yucatan. The wide range of size and distribution of the captured specimens, including the smallest specimen found in the western Atlantic and the presence of females at an advanced stage of gonadal maturity, indicates that Penaeus monodon has established itself in the area. The possible negative effects of such establishment make necessary the proposal of measures based on scientific evidence.

The Maturity of Female Gonads of G2 Transgenic Mutiara Catfish (Clarias sp.)

The G2 trangsenic Mutiara catfish (Clarias sp.) (MTG) is a Mutiara catfish that is inserted with the CgGH gene (Clarias gariepinus Growth Hormone) through the transgenesis. The effect of transgenesis stimulates gonad growth of G2 transgenic Mutiara catfish (Clarias sp.) faster than non-transgenic fish. Study aimed to analyze the maturity of the gonads and the spawning ability of female G2 transgenic Mutiara catfish (Clarias sp.) to obtain superior broodstock candidates. Experimental method with completely randomized design (pair of parents used as treatment and repeated four times) for spawning was used for this study. Three pairs of parent G2 were crossed semi-artificially as treatment A (female 1 MTG G2 crossed with male 1 MTG G2), B (female 2 MTG G2 crossed with male 2 MTG G2) and C (female 3 MTG G2 crossed with male 3 MTG G2). The results showed that the performance of female G2 transgenic Mutiara catfish (Clarias sp.) (treatment A, B and C) was higher given non-transgenic with an average relative fecundity of 82,438 eggs / kg of broodstock, an average egg diameter of 1.76 mm and an average egg weight 1.75 mg. These indications suggest that GH transgenesis increases gonadal maturity. The gonad maturity profile reached the stage of complete maturity (full ripe) compared to Sangkuriang catfish (Clarias gariepinus) (immature gonads). Therefore it is necessary to compare the reproductive performance of G2 transgenic Mutiara catfish (Clarias sp.) with non-transgenic fish (Sangkuriang) as candidates for superior broodstock of catfish.

Some Biological Aspects of Cultured Ompok Pabda (Hamilton, 1822) Collected from A Local Fish Farm in Mymensingh, Bangladesh

An experiment was conducted to observe some biological aspects including growth, sex ratio, gonadal maturity, artificial insemination, fecundity, fertilization, hatching and larval development of O. pabda in a private hatchery. Total weight of the sampled fish varied from 1.60±0.43 to 111.12±6.83 g and length varied from 3.57±0.65 to 21.22±1.84 cm with the SGR 2.85±0.18 and ADG 0.73±0.05. The length and weight of the fish had a strong correlation (r²=0.966). Female dominance over male has been observed in the species. The male to female ratio was 1:1.48. Females were 16.8 cm in length at first maturity, while males were 16.6 cm in length. The GSI of female were ranged from 2.93 to 4.77 with the mean of 3.77±0.60 during the study period. The fecundity of O. pabda was ranged from 5675 to 19626 with the mean of 12503±4192 for the corresponding length 21.23±1.71, body weight 111.12±7.6 and gonad weight 4.19±0.87. Fertilization rate, hatching rate and larval development observed during the experiment. Scattered diagram for length-weight, fecundity-length, fecunditybody weight and fecundity-gonad weight relationship shows a perfect correlation both arithmetically and logarithmically.