Enhancement of Reflection-Enhanced Backscatter Confocal Microscopy

2001 ◽  
Vol 7 (S2) ◽  
pp. 1014-1015
Author(s):  
C. H. Keith ◽  
R. Witek
Keyword(s):  
Refractive Index ◽  
Optical Resolution ◽  
Differential Interference Contrast ◽  
Reflection Mode ◽  
Metallic Substrates ◽  
Silicon Chips ◽  
Light Mode ◽  
Living Neurons ◽  
Growth Of Bacteria ◽  
Made In

Tremendous advances have been made in the last twenty years in extending the usefulness of the light microscope in visualizing living biological specimens, and it is now possible to visualize most thin tissues in an unstained state at – or near – the limit of optical resolution. in general, however, these techniques have been used successfully on biological specimens in the transmitted light mode; unstained biological specimens generally do not have sufficient albedo or sufficient difference in refractive index from their surroundings to be efficiently visualized in epi-illumination. A number of applications, including studies of the growth of bacteria on metallic substrates, and studies of the studies of the formation of interfaces between living neurons and silicon chips, would benefit from being able to resolve living, unstained biological specimens growing on opaque substratesWe found that by imaging biological specimens on a reflective substrate in the reflection mode of the confocal microscope, we could get images that resemble transmission mode differential interference contrast images.

Enhancement of optical resolution in three-dimensional refractive-index tomograms of biological samples by employing micromirror-embedded coverslips

Lab on a Chip ◽  
2018 ◽  
Vol 18 (22) ◽  
pp. 3484-3491 ◽  
Author(s):  
Seungwoo Shin ◽  
Jihye Kim ◽  
Je-Ryung Lee ◽  
Eun-chae Jeon ◽  
Tae-Jin Je ◽  
...  
Keyword(s):  
Refractive Index ◽  
Biological Samples ◽  
Three Dimensional ◽  
Optical Resolution ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography

Resolution-enhanced optical diffraction tomography using a micromirror-embedded coverslips.

scholarly journals Microstructured Optical Waveguide-Based Endoscopic Probe Coated with Silica Submicron Particles

Materials ◽  
2019 ◽  
Vol 12 (9) ◽  
pp. 1424 ◽  
Author(s):  
Timur Ermatov ◽  
Yury V. Petrov ◽  
Sergei V. German ◽  
Anastasia A. Zanishevskaya ◽  
Andrey A. Shuvalov ◽  
...  
Keyword(s):  
Refractive Index ◽  
Optical Waveguides ◽  
Silica Particles ◽  
Submicron Particles ◽  
Layer By Layer ◽  
Biological Sensing ◽  
Filling Materials ◽  
Diallyldimethylammonium Chloride ◽  
Light Mode ◽  
Different Diameters

Microstructured optical waveguides (MOW) are of great interest for chemical and biological sensing. Due to the high overlap between a guiding light mode and an analyte filling of one or several fiber capillaries, such systems are able to provide strong sensitivity with respect to variations in the refractive index and the thickness of filling materials. Here, we introduce a novel type of functionalized MOWs whose capillaries are coated by a layer-by-layer (LBL) approach, enabling the alternate deposition of silica particles (SiO2) at different diameters—300 nm, 420 nm, and 900 nm—and layers of poly(diallyldimethylammonium chloride) (PDDA). We demonstrate up to three covering bilayers consisting of 300-nm silica particles. Modifications in the MOW transmission spectrum induced by coating are measured and analyzed. The proposed technique of MOW functionalization allows one to reach novel sensing capabilities, including an increase in the effective sensing area and the provision of a convenient scaffold for the attachment of long molecules such as proteins.

Coating Thickness Characterization of Composite Materials Using Terahertz Waves

2016 ◽  
Vol 878 ◽  
pp. 70-73 ◽  
Author(s):  
Kwang Hee Im ◽  
Sun Kyu Kim ◽  
David K. Hsu ◽  
Jong An Jung
Keyword(s):  
Refractive Index ◽  
Coating Thickness ◽  
Composite Laminates ◽  
Electromagnetic Properties ◽  
Terahertz Waves ◽  
Reflection Mode ◽  
Cfrp Composite ◽  
Spectroscopy System ◽  
Terahertz Ray

Recently, terahertz ray imaging has emerged as one of the most promising new powerful nondestructive evaluation (NDE) techniques for the area applications. In this study, a new time-domain spectroscopy system was utilized for measuring the coating thickness on CFRP composite laminates. Extensive experimental measurements in reflection mode were made to map out the T-ray images. Also, the refractive index was estimated based on the electromagnetic properties. The CFRP composite laminates were observed in reflection mode and limitations will be discussed in the T-ray processing. By using these characterized material properties, the characteristics was successfully demonstrated for T-ray behavior propagating through the Shim Stock films for acquiring the refractive index. The T-ray technique has been developed for the measurement of the thickness of the Shim Stock films and the coating thickness on CFRP composites. Good results have been obtained in tests made on the thickness of the standard film samples with the coating thickness ranging from around hundreds of μm.

scholarly journals Reflection-mode multifocal optical-resolution photoacoustic microscopy

2013 ◽  
Vol 18 (03) ◽  
pp. 1 ◽  
Author(s):  
Guo Li ◽  
Konstantin I. Maslov ◽  
Lihong V. Wang
Keyword(s):  
Optical Resolution ◽  
Photoacoustic Microscopy ◽  
Reflection Mode

Ellipsometric investigation of the electro-optic and electrostrictive effects in anodic Ta 2 O 5 films

1973 ◽  
Vol 335 (1600) ◽  
pp. 39-50 ◽  
Keyword(s):  
Refractive Index ◽  
Relaxation Time ◽  
Sulphuric Acid ◽  
Oxide Films ◽  
Rapid Change ◽  
Anodic Oxide ◽  
Zero Field ◽  
Electro Optic ◽  
Computer Controlled ◽  
Made In

A computer-controlled ellipsometer was used to detect and measure the increase in thickness, the decrease in refractive index and the development of birefringence which occur on applying fields up to a few hundred MV m ‒1 to anodic oxide films made in dilute sulphuric acid on tantalum. The films were shown to be optically isotropic and homogeneous at zero field but to be optically anisotropic with field applied. There is a rapid change in index and thickness when a field is first applied, followed by a slower change with relaxation time of the order of 10 s. Both the index and the thickness changes are quadratic in the field, as is to be expected for a material which is isotropic with no field applied.

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