Sampling-Resolution Strategy for One-Way Multiplexed Immunoassay with Sequential Chemiluminescent Detection

Physical mistreatment has been estimated to affect 2 million older persons each year and dramatically affects health outcomes. While researchers have attempted to examine risk factors for specific forms of abuse, many have been able to focus on only victim or perpetrator characteristics, or a limited number of psychosocial variables at any one time. Additionally, data on risk factors for subgroups such as persons with Alzheimer’s disease who may have heightened and/or unique risk profiles has also been limited. This paper examines risk for physical violence in caregiver/patient dyads who participated in the Aggression and Violence in Community-Based Alzheimer’s Families Grant. Data were collected via in-person interview and mailed survey and included demographics as well as measures of violence, physical and emotional health, and health behaviors. Logistic regression analysis indicated that caregivers providing care to elders with high levels of functional impairment or dementia symptoms, or who had alcohol problems, were more likely to use violence as a conflict resolution strategy, as were caregivers who were providing care to elders who used violence against them. By contrast, caregivers with high self-esteem were less likely to use violence as a conflict resolution strategy. Significant interaction effects were also noted.

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Parent-adolescent conflict and choice of conflict resolution strategy

International Journal of Conflict Management ◽

10.1108/ijcma-04-2014-0025 ◽

2016 ◽

Vol 27 (1) ◽

pp. 88-115 ◽

Cited By ~ 8

Author(s):

Rashmi Singh ◽

J. K. Nayak

Keyword(s):

Factor Analysis ◽

Conflict Resolution ◽

Structural Equation ◽

Content Type ◽

Conflict Resolution Strategies ◽

Adolescent Conflict ◽

Conflict Resolution Strategy ◽

Resolution Strategy ◽

The Impact ◽

Resolution Strategies

Purpose – The purpose of this paper is to investigate the impact of family communication patterns (FCPs) on adolescents’ choice of conflict resolution strategies during family holiday planning. Design/methodology/approach – The paper is framed by and builds on the literature on the adolescents’ choice of conflict resolution strategies and the FCP. The sample was collected using a survey among 400 adolescents in India. Exploratory factor analysis, confirmatory factor analysis and structural equation modelling have been used to analyse the data. Findings – The paper provides a significant relationship between FCP and the choice of conflict resolution strategies. The study findings suggested that adolescents’ choice of resolution strategy depends on the type of communication environment in the family. It has been seen that adolescents have more say in concept-oriented families and use different types of resolution strategies compared to socio-oriented families. Practical implications – The present study has considerable implications for the marketers and the academicians. Through FCP, marketers will be able to segment the families and, hence, may introduce efficient and competent marketing strategies and promotional campaigns. Originality/value – The paper offers insights into the choice of conflict resolution strategy by adolescents’ in different FCPs. The study has originality and offers value to marketers as it focuses on adolescents, and explores their perceptions about their influence during the decision process.

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Magnetic covalent organic framework immobilized gold nanoparticles with high-efficiency catalytic performance for chemiluminescent detection of pesticide triazophos

Talanta ◽

10.1016/j.talanta.2021.122798 ◽

2021 ◽

pp. 122798

Author(s):

Yuyu Ma ◽

Yaxin Zhao ◽

Xiaotong Xu ◽

Shujiang Ding ◽

Yinhuan Li

Keyword(s):

Gold Nanoparticles ◽

High Efficiency ◽

Catalytic Performance ◽

Covalent Organic Framework ◽

Chemiluminescent Detection ◽

Organic Framework

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Development of All-Plastic Microvalve Array for Multiplexed Immunoassay

Volume 10: Micro- and Nano-Systems Engineering and Packaging ◽

10.1115/imece2014-38154 ◽

2014 ◽

Author(s):

Shancy Augustine ◽

Pan Gu ◽

Xiangjun Zheng ◽

Toshikazu Nishida ◽

Z. Hugh Fan

Keyword(s):

Large Scale ◽

Low Cost ◽

Simultaneous Detection ◽

Cost Effective ◽

Negative Control ◽

Sensitive Material ◽

Detection Scheme ◽

Power Battery ◽

Multiple Analytes ◽

Multiplexed Immunoassay

There is a need for low-cost immunoassays that measure the presence and concentration of multiple harmful agents in one device. Currently, comparable immunoassays employ a one-analyte-per-test format that is time consuming and not cost effective for the requirement of detecting multiple analytes in a single sample. For instance, if a spectrum of harmful agents, including E. coli O157, cholera toxin, and Salmonella typhimurium, should be simultaneously monitored in foods and drinking water, then a one-analyte-per-test would be inefficient. This work demonstrates a platform capable of simultaneous detection of multiple analytes in a single, low-cost, microvalve array-enabled multiplexed immunoassay. This multiplexed immunoassay platform is demonstrated in a prototype COC (cyclic olefin copolymer) device with a 2×3 array in which 6 analytes can be detected simultaneously. In order to contain and regulate the flow of reagents in the multichannel device, an array of microfluidic valves actuated by a thermally expandable material and microfabricated resistors have been developed to direct the flow to the necessary assay sites. The microvalve-based immunoassay is shown to be reliable, easy to operate, and compatible with large-scale integration. The all-plastic microvalves use paraffin wax as the thermally sensitive material which drastically reduces power consumption by latching upon closing so that pulsed power is required only to close and latch the microvalve until it is necessary to re-open the valve. The multiplexed detection scheme has been demonstrated by using three proteins, C reactive protein (CRP) and transferrin, both of which are biomarkers associated with traumatic brain injury (TBI) as well as bovine serum albumin (BSA) as the negative control. Since there are no external bulky pneumatic accessories required to operate/latch the microvalves in the device, this compact, thermally actuated and latching microvalve-enabled multiplexed immunoassay has the potential to realize a portable, low power, battery operated microfluidic device for biological assays.

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