SiO2 Biogenic Nanoparticles and Asphaltenes: Interactions and Their Consequences Investigated by QCR and GPC-ICP-HR-MS

Infectious diseases pose one of the greatest health challenges in the medical world. Though numerous antimicrobial drugs are commercially available, they often lack effectiveness against recently developed multidrug resistant (MDR) microorganisms. This results in high antibiotic dose administration and a need to develop new antibiotics, which in turn requires time, money, and labor investments. Recently, biogenic metallic nanoparticles have proven their effectiveness against MDR microorganisms, individually and in synergy with the current/conventional antibiotics. Importantly, biogenic nanoparticles are easy to produce, facile, biocompatible, and environmentally friendly in nature. In addition, biogenic nanoparticles are surrounded by capping layers, which provide them with biocompatibility and long-term stability. Moreover, these capping layers provide an active surface for interaction with biological components, facilitated by free active surface functional groups. These groups are available for modification, such as conjugation with antimicrobial drugs, genes, and peptides, in order to enhance their efficacy and delivery. This review summarizes the conventional antibiotic treatments and highlights the benefits of using nanoparticles in combating infectious diseases.

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Investigation of the Antimicrobial Activity and Hematological Pattern of Nano-Chitosan and its Developed Nano-Copper Composite

10.21203/rs.3.rs-149486/v1 ◽

2021 ◽

Author(s):

Somia Ahmed ◽

Hadeer Mohamed ◽

Abeer Al-Subaie ◽

Ahoud Al-Ohali ◽

Nesrine Mahmoud

Keyword(s):

Antimicrobial Activity ◽

High Sensitivity ◽

Ionic Gelation ◽

Clotting Time ◽

Blood Samples ◽

Gram Negative ◽

Inhibition Concentration ◽

Copper Composite ◽

Biogenic Nanoparticles

Abstract Novel synthesized Chitosan-Copper oxide nanocomposite (Cs-CuO) was prepared using pomegranate peels extract as green precipitating agents to improve the biological activity of Cs-NP's which was synthesized through ionic gelation method. The characterization of biogenic nanoparticles Cs-NP's and Cs-CuO-NP's were investigated structurally, morphologically to determine the full descriptive features of those nanoparticles. Antimicrobial activity was tested for both Cs-NP's and Cs-CuO-NP's via Minimum inhibition concentration and zone analysis against fungus, gram positive and gram negative. The results of the antimicrobial test showed high sensitivity of Cs-CuO-NP's to all microorganisms that are tested in concentration less than 20 mg/ml while the sensitivity of Cs-NP's against all microorganisms under test started from a concentration of 20 mg/ml to 40 mg/ml except for the C.albicans species. Hematological activity was also tested in via measuring the RBCs, platelets count and clotting time against healthy, diabetic and hypercholesterolic blood samples. Measurement showed a decrease in RBCs and platelets count by adding Cs-NP’s or Cs-CuO-NP's to the three blood samples. Cs-NP’s success to decrease the clotting time for healthy and diabetic blood acting as a procoagulant agent, while adding biogenic CuO-NP’s to Cs-NP’s increased clotting time considering as an anticoagulant agent for hyperchloesterolic blood samples.

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Diverse Manifolds of Biogenic Nanoparticles in Synthesis, Characterization, and Applications

Nanotechnology in the Life Sciences - Nanotechnology Applications in Health and Environmental Sciences ◽

10.1007/978-3-030-64410-9_1 ◽

2021 ◽

pp. 1-28

Author(s):

Jeyaraj Pandiarajan

Keyword(s):

Biogenic Nanoparticles

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Detection of Microorganisms in Low-Temperature Water Environments by in situ Generation of Biogenic Nanoparticles

Frontiers in Astronomy and Space Sciences ◽

10.3389/fspas.2020.00059 ◽

2020 ◽

Vol 7 ◽

Author(s):

Dmitry A. Skladnev ◽

Lina V. Vasilyeva ◽

Yulia Yu. Berestovskaya ◽

Oleg R. Kotsyurbenko ◽

Sergei V. Kalenov ◽

...

Keyword(s):

Low Temperature ◽

Water Samples ◽

Sampling Site ◽

Ag Nps ◽

Biological Objects ◽

In Situ Generation ◽

The Comparative Study ◽

Biogenic Nanoparticles ◽

Temperature Water

A new nanobiotechnological approach for the detection of extraterrestrial Earth-like biological forms is proposed. The approach is based on the ability of microbial cells to reduce artificially added cations with the generation of crystalline nanoparticles (NPs) from zero-valent atoms. The method is named DBNG (Detection of Biogenic Nanoparticles Generation). The subglacial low-temperature oligotrophic Lake Untersee in Antarctica was used as a model of putative extraterrestrial water environments inhabited by Earth-like type microorganisms. The DBNG protocol for the comparative study of microbial communities of low-temperature oligotrophic environments was optimized on the base of experiments with the pure culture of psychroactive bacterium Cryobacterium sp. 1639 isolated earlier from Lake Untersee. The formation of silver nanoparticles (Ag°NPs) has been conducted in natural water samples of three horizons at low temperature (+5°C), which was in the temperature range registered in the Lake Untersee. The generation of biogenic Ag°NPs was detected only at the presence of indigenous microorganisms in all studied samples. No Ag°NPs generation was observed in the lake water samples artificially free of cells or exposed to pasteurization (two types of controls). The miniature microfluidic chip for an automated version of the device, based on using different analytical methods for recording in situ-formed biogenic nanoparticles, is proposed. The device allows the detection of the biological objects directly at the sampling site.

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