Correction to “Intranuclear Drug Delivery and Effective in Vivo Cancer Therapy via Estradiol–PEG-Appended Multiwalled Carbon Nanotubes”

Abstract Background Lung cancer is one of the important health threats worldwide, of which 5-year survival rate is less than 15%. Non-small-cell lung cancer (NSCLC) accounts for about 80% of all lung cancer with high metastasis and mortality. Methods Cisplatin loaded multiwalled carbon nanotubes (Pt-MWNTS) were synthesized and used to evaluate the anticancer effect in our study. The NSCLC cell lines A549 (cisplatin sensitive) and A549/DDP (cisplatin resistant) were used in our in vitro assays. MTT was used to determine Cancer cells viability and invasion were measured by MTT assay and Transwell assay, respectively. Apoptosis and epithelial-mesenchymal transition related marker proteins were measured by western blot. The in vivo anti-cancer effect of Pt-MWNTs were performed in male BALB/c nude mice (4-week old). Results Pt-MWNTS were synthesized and characterized by X-ray diffraction, Raman, FT-IR spectroscopy and scan electron microscopy. No significant cytotoxicity of MWNTS was detected in both A549/DDP and A549 cell lines. However, Pt-MWNTS showed a stronger inhibition effect on cell growth than free cisplatin, especially on A549/DDP. We found Pt-MWNTS showed higher intracellular accumulation of cisplatin in A549/DDP cells than free cisplatin and resulted in enhanced the percent of apoptotic cells. Western blot showed that application of Pt-MWNTS can significantly upregulate the expression level of Bax, Bim, Bid, Caspase-3 and Caspase-9 while downregulate the expression level of Bcl-2, compared with free cisplatin. Moreover, the expression level of mesenchymal markers like Vimentin and N-cadherin was more efficiently reduced by Pt-MWNTS treatment in A549/DDP cells than free cisplatin. In vivo study in nude mice proved that Pt-MWNTS more effectively inhibited tumorigenesis compared with cisplatin, although both of them had no significant effect on body weight. Conclusion Pt-MWNT reverses the drug resistance in the A549/DDP cell line, underlying its possibility of treating NSCLC with cisplatin resistance.

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Glycopolymer decorated multiwalled carbon nanotubes for dual targeted breast cancer therapy

Journal of Materials Chemistry B ◽

10.1039/c9tb02711d ◽

2020 ◽

Vol 8 (15) ◽

pp. 3123-3137 ◽

Cited By ~ 10

Author(s):

Pinar Sinem Omurtag Ozgen ◽

Sezen Atasoy ◽

Belma Zengin Kurt ◽

Zehra Durmus ◽

Gulsah Yigit ◽

...

Keyword(s):

Breast Cancer ◽

Carbon Nanotubes ◽

Cancer Therapy ◽

Multiwalled Carbon Nanotubes ◽

Biomedical Applications ◽

Cancer Imaging ◽

Breast Cancer Therapy ◽

Multiwalled Carbon ◽

Carbon Based Nanomaterials ◽

Carbon Based

Carbon-based nanomaterials (CNMs) have attracted great attention in biomedical applications such as cancer imaging and therapy.

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Fabrication of multiwalled carbon nanotubes/carrageenan-chitosan@ Ce and Sr substituted hydroxyapatite biocomposite coating on titanium: In vivo bone formation evaluations

Journal of King Saud University - Science ◽

10.1016/j.jksus.2019.11.006 ◽

2020 ◽

Vol 32 (1) ◽

pp. 1175-1181 ◽

Cited By ~ 3

Author(s):

Meng Chen ◽

Haoxuan Zhang ◽

Shiying Shan ◽

Yuanyuan Li ◽

Xiaoguang Li ◽

...

Keyword(s):

Carbon Nanotubes ◽

Bone Formation ◽

Multiwalled Carbon Nanotubes ◽

Multiwalled Carbon ◽

In Vivo Bone Formation

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Comparative in vivo toxicity assessment places multiwalled carbon nanotubes at a higher level than mesoporous silica nanoparticles

Toxicology and Industrial Health ◽

10.1177/0748233715622307 ◽

2016 ◽

Vol 33 (2) ◽

pp. 182-192 ◽

Cited By ~ 11

Author(s):

Nidhi Rawat ◽

Sandhya ◽

Kesavan Subaharan ◽

M Eswaramoorthy ◽

Gautam Kaul

Keyword(s):

Carbon Nanotubes ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Multiwalled Carbon Nanotubes ◽

Mesoporous Silica Nanoparticles ◽

Treated Group ◽

Cellular Level ◽

Multiwalled Carbon ◽

In Vivo Toxicity

In the present work, we took two nanomaterials (NMs), mesoporous silica nanoparticles (MSNs) and multiwalled carbon nanotubes (MWCNTs), and compared their in vivo toxicity taking albino mice as a test animal model. Presently, conflicting data persist regarding behavior of these NMs with macromolecules like protein and lipid at the cellular level in cell lines as well as in animal models and this generated the interest to study them. The mice were treated orally with a single dose of 50 ppm MWCNTs and intraperitoneally with 10, 25, and 50 mg kg−1 body weight (BW) of MSNs and 1.5, 2.0, and 2.5 mg kg−1 BW of MWCNTs. Liver enzyme markers serum aspartate aminotransferase (AST), alanine aminotransferase, and alkaline phosphatase along with total protein (TP) levels were evaluated 7 days postexposure. No significant differences in organ weight indices or enzyme levels were observed between different treatment doses but there were significant differences between the treatment groups and the controls. Of the three enzymes assayed, AST displayed a peculiar pattern, especially in the MWCNTs intraperitoneally treated group. TP level was significantly increased in the orally treated MWCNTs group. The results showed that MWCNTs even at much smaller doses than MSNs displayed similar toxicity levels, suggesting that toxicity of MWCNTs is greater than MSNs.

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Preparation, Characterization, andIn VitroandVivoAntitumor Activity of Oridonin-Conjugated Multiwalled Carbon Nanotubes Functionalized with Carboxylic Group

Journal of Nanomaterials ◽

10.1155/2016/3439419 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Chuanjin Wang ◽

Wei Li

Keyword(s):

Carbon Nanotubes ◽

Multiwalled Carbon Nanotubes ◽

Tumor Therapy ◽

Water Soluble ◽

Carboxylic Group ◽

Multiwalled Carbon ◽

Compound C ◽

Poorly Water Soluble

Carbon nanotubes have shown great potential in tumor therapy. Oridonin (ORI) is a poorly water-soluble diterpenoid compound (C20H28O6) used in the treatment of esophageal and hepatic carcinoma for decades. For the purpose of enhancing the antitumor potency and reducing cytotoxicity of ORI, multiwalled carbon nanotubes functionalized with carboxylic group (MWCNTs-COOH) were used as ORI carrier. ORI was noncovalently encapsulated into (or onto) the functionalized carbon nanotubes (MWCNTs-ORI). The obtained MWCNTs-ORI has been characterized. The ORI loading efficiency in MWCNTs-COOH carrier was studied to be about 82.6% (w/w).In vitrocytotoxicity assay on MWCNTs-ORI gave IC50of7.29±0.5 μg/mL and ORI-F gave IC50of14.5±1.4 μg/mL. The antitumor effect studiesin vivoshowed that MWCNTs-ORI improved antitumor activity of ORI in comparison with ORI-F. The tumor inhibition ratio for MWCNTs-ORI (1.68×10-2 g·Kg−1·d−1) was 86.4%, higher than that of ORI-F (1.68×10-2 g·Kg−1·d−1) which was 39.2%. This can greatly improve the pharmaceutical efficiency and reduce potential side effects.

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Poly(3-Hydroxybutyrate)-Multiwalled Carbon Nanotubes Electrospun Scaffolds Modified with Curcumin

Polymers ◽

10.3390/polym12112588 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2588

Author(s):

Nader Tanideh ◽

Negar Azarpira ◽

Najmeh Sarafraz ◽

Shahrokh Zare ◽

Aida Rowshanghiyas ◽

...

Keyword(s):

Carbon Nanotubes ◽

Tissue Engineering ◽

Multiwalled Carbon Nanotubes ◽

Inflammatory Reaction ◽

3D Structure ◽

Hydrolytic Degradation ◽

Multiwalled Carbon ◽

Electrospun Scaffolds

Appropriate selection of suitable materials and methods is essential for scaffolds fabrication in tissue engineering. The major challenge is to mimic the structure and functions of the extracellular matrix (ECM) of the native tissues. In this study, an optimized 3D structure containing poly(3-hydroxybutyrate) (P3HB), multiwalled carbon nanotubes (MCNTs) and curcumin (CUR) was created by electrospinning a novel biomimetic scaffold. CUR, a natural anti-inflammatory compound, has been selected as a bioactive component to increase the biocompatibility and reduce the potential inflammatory reaction of electrospun scaffolds. The presence of CUR in electrospun scaffolds was confirmed by 1H NMR and Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) revealed highly interconnected porosity of the obtained 3D structures. Addition of up to 20 wt% CUR has enhanced mechanical properties of the scaffolds. CUR has also promoted in vitro bioactivity and hydrolytic degradation of the electrospun nanofibers. The developed P3HB-MCNT composite scaffolds containing 20 wt% of CUR revealed excellent in vitro cytocompatibility using mesenchymal stem cells and in vivo biocompatibility in rat animal model study. Importantly, the reduced inflammatory reaction in the rat model after 8 weeks of implantation has also been observed for scaffolds modified with CUR. Overall, newly developed P3HB-MCNTs-CUR electrospun scaffolds have demonstrated their high potential for tissue engineering applications.

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