Equilibrium Unfolding (Folding) Pathway of a Model H-type Pseudoknotted RNA:  The Role of Magnesium Ions in Stability†

Biochemistry ◽  
1998 ◽  
Vol 37 (46) ◽  
pp. 16116-16129 ◽  
Author(s):  
Paul L. Nixon ◽  
David P. Giedroc
Keyword(s):  
Folding Pathway ◽  
Magnesium Ions ◽  
Equilibrium Unfolding

The Role of Calcium and Magnesium Ions in Uptake of β-Amyloid Peptides by Microglial Cells

2006 ◽  
Vol 19 (3) ◽  
pp. 683-696 ◽  
Author(s):  
N. Choucair ◽  
V. Laporte ◽  
R. Levy ◽  
C. Tranchant ◽  
J.-P. Gies ◽  
...  
Keyword(s):  
Microglial Cells ◽  
Magnesium Ions ◽  
Amyloid Peptides ◽  
Β Amyloid ◽  
Calcium And Magnesium ◽  
Calcium And Magnesium Ions

The role of magnesium ions in bone regeneration involves the canonical Wnt signaling pathway

2019 ◽  
Vol 98 ◽  
pp. 246-255 ◽  
Author(s):  
Chu-Chih Hung ◽  
Amy Chaya ◽  
Kai Liu ◽  
Konstantinos Verdelis ◽  
Charles Sfeir
Keyword(s):  
Wnt Signaling ◽  
Bone Regeneration ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Magnesium Ions ◽  
Canonical Wnt Signaling ◽  
Canonical Wnt

scholarly journals The role of an evolutionarily conserved cis-proline in the thioredoxin-like domain of human class Alpha glutathione transferase A1-1

2003 ◽  
Vol 372 (1) ◽  
pp. 241-246 ◽  
Author(s):  
Chris NATHANIEL ◽  
Louise A. WALLACE ◽  
Jonathan BURKE ◽  
Heini W. DIRR
Keyword(s):  
Glutathione Transferase ◽  
Conformational Stability ◽  
Wild Type ◽  
Catalytic Function ◽  
Evolutionarily Conserved ◽  
Glutathione S Transferases ◽  
Equilibrium Unfolding ◽  
The Stability ◽  
Unfolding Kinetics

The thioredoxin-like fold has a βαβαββα topology, and most proteins/domains with this fold have a topologically conserved cis-proline residue at the N-terminus of β-strand 3. This residue plays an important role in the catalytic function and stability of thioredoxin-like proteins, but is reported not to contribute towards the stability of glutathione S-transferases (GSTs) [Allocati, Casalone, Masulli, Caccarelli, Carletti, Parker and Di Ilio (1999) FEBS Lett. 445, 347–350]. In order to further address the role of the cis-proline in the structure, function and stability of GSTs, cis-Pro-56 in human GST (hGST) A1-1 was replaced with a glycine, and the properties of the P56G mutant were compared with those of the wild-type protein. Not only was the catalytic function of the mutant dramatically reduced, so was its conformational stability, as indicated by equilibrium unfolding and unfolding kinetics experiments with urea as denaturant. These findings are discussed in the context of other thioredoxin-like proteins.

scholarly journals Influence of Magnesium Ions on the Preparation and Storage of DNA Tetrahedrons in Micromolar Ranges

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2091 ◽  
Author(s):  
Yue Hu ◽  
Zhou Chen ◽  
Zheng Hou ◽  
Mingkai Li ◽  
Bo Ma ◽  
...  
Keyword(s):  
The Novel ◽  
Magnesium Ions ◽  
Dna Nanostructures ◽  
Structure And Stability ◽  
Dna Concentration ◽  
Factors Influencing ◽  
Dna Tetrahedron ◽  
And Storage

The DNA tetrahedron (Td), as one of the novel DNA-based nanoscale biomaterials, has been extensively studied because of its excellent biocompatibility and increased possibilities for decorating precisely. Although the use of Td in laboratories is well established, knowledge surrounding the factors influencing its preparation and storage is lacking. In this research, we investigated the role of the magnesium ions, which greatly affect the structure and stability of DNA. We assembled 1, 2, 5, 10 and 20 μM Td in buffers containing different Mg2+ concentrations, demonstrating that 2 and 5 mM Mg2+ is optimal in these conditions, and that yields decrease dramatically once the DNA concentration reaches 20 μM or the Mg2+ concentration is lower than 0.5 mM. We also verified that the Td structure is retained better through freeze-thawing than lyophilization. Furthermore, a lower initial Mg2+ (≤2 mM) benefited the maintenance of Td structure in the process of lyophilization. Hence, our research sheds light on the influence of Mg2+ in the process of preparing and storing Td, and also provides some enlightenment on improving yields of other DNA nanostructures.

Role of magnesium ions in DNA recognition by the EcoRV restriction endonuclease

FEBS Letters ◽  
2011 ◽  
Vol 585 (17) ◽  
pp. 2739-2743 ◽  
Author(s):  
Mai Zahran ◽  
Tomasz Berezniak ◽  
Petra Imhof ◽  
Jeremy C. Smith
Keyword(s):  
Restriction Endonuclease ◽  
Dna Recognition ◽  
Magnesium Ions

Role of Calcium and Magnesium Ions in the Hardening of Pressure-Treated Root Vegetables

1997 ◽  
Vol 45 (3) ◽  
pp. 599-603 ◽  
Author(s):  
Midori Kasai ◽  
Nagomi Okamoto ◽  
Keiko Hatae ◽  
Atsuko Shimada
Keyword(s):  
Magnesium Ions ◽  
Calcium And Magnesium ◽  
Calcium And Magnesium Ions ◽  
Root Vegetables

scholarly journals Resorbable Mg2+-Containing Phosphates for Bone Tissue Repair

2021 ◽  
Author(s):  
Gilyana Kazakova ◽  
Tatiana Safronova ◽  
Daniil Golubchikov ◽  
Olga Shevtsova ◽  
Julietta Rau
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Repair ◽  
Early Stage ◽  
Biological Properties ◽  
Magnesium Ions ◽  
Comprehensive Overview ◽  
Phosphate Ions ◽  
Great Relevance

Materials based on Mg2+-containing phosphates are gaining great relevance in the field of bone tissue repair via regenerative medicine methods. Magnesium ions, together with condensed phosphate ions, play a significant role in the process of bone remodeling, affecting the early stage of bone regeneration through active participation in the process of osteosynthesis. Here we pro-vide a comprehensive overview of the usage of biomaterials based on magnesium phosphate and magnesium calcium phosphate in bone reconstruction. The role of magnesium ions in angiogene-sis, an important process associated with osteogenesis, is considered. Finally, the biological properties of magnesium phosphates for bone regeneration are summarized. They show promis-ing results in terms of use as bone replacement material.

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