Characterization of High-Boiling-Solvent Lignin from Hot-Water-Extracted Bagasse

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

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Structural Characterization of a Polysaccharide from Gastrodia elata and Its Bioactivity on Gut Microbiota

Molecules ◽

10.3390/molecules26154443 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4443

Author(s):

Jiangyan Huo ◽

Min Lei ◽

Feifei Li ◽

Jinjun Hou ◽

Zijia Zhang ◽

...

Keyword(s):

Molecular Weight ◽

Citric Acid ◽

Gut Microbiota ◽

Structural Characterization ◽

Hot Water ◽

Nmr Analysis ◽

Gastrodia Elata ◽

Hot Water Extraction ◽

Hydroxybenzyl Alcohol

A novel homogeneous polysaccharide named GEP-1 was isolated and purified from Gastrodia elata (G. elata) by hot-water extraction, ethanol precipitation, and membrane separator. GEP-1, which has a molecular weight of 20.1 kDa, contains a polysaccharide framework comprised of only glucose. Methylation and NMR analysis showed that GEP-1 contained 1,3,6-linked-α-Glcp, 1,4-linked-α-Glcp, 1,4-linked-β-Glcp and 1,4,6-linked-α-Glcp. Interestingly, GEP-1 contained citric acid and repeating p-hydroxybenzyl alcohol as one branch. Furthermore, a bioactivity test showed that GEP-1 could significantly promote the growth of Akkermansia muciniphila (A. muciniphila) and Lacticaseibacillus paracasei (L.paracasei) strains. These results implied that GEP-1 might be useful for human by modulating gut microbiota.

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Chemical Characterization of the High Molecular Weight Material Extracted with Hot Water from Green and Roasted Arabica Coffee

Journal of Agricultural and Food Chemistry ◽

10.1021/jf0012953 ◽

2001 ◽

Vol 49 (4) ◽

pp. 1773-1782 ◽

Cited By ~ 83

Author(s):

Fernando M. Nunes ◽

Manuel A. Coimbra

Keyword(s):

Molecular Weight ◽

High Molecular Weight ◽

Chemical Characterization ◽

Hot Water ◽

Arabica Coffee ◽

High Molecular Weight Material

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Characterization of Value-Added Non-Carbohydrate Compounds Solubilized during Acidic Hot Water Flowthrough Pretreatment of Poplar Wood

BioResources ◽

10.15376/biores.12.1.899-911 ◽

2016 ◽

Vol 12 (1) ◽

Author(s):

Lishi Yan ◽

Ruoshui Ma ◽

Quan Bu ◽

Liangzhi Li ◽

Xiao Zhou ◽

...

Keyword(s):

Value Added ◽

Hot Water ◽

Poplar Wood ◽

Flowthrough Pretreatment

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Characterization of proteins released from legume seeds in hot water

Phytochemistry ◽

10.1016/0031-9422(92)80149-9 ◽

1992 ◽

Vol 31 (2) ◽

pp. 731-735 ◽

Cited By ~ 10

Author(s):

H Hirano

Keyword(s):

Hot Water ◽

Legume Seeds

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Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization

Molecules ◽

10.3390/molecules24152740 ◽

2019 ◽

Vol 24 (15) ◽

pp. 2740 ◽

Cited By ~ 18

Author(s):

Ekaterina Baeva ◽

Roman Bleha ◽

Ekaterina Lavrova ◽

Leonid Sushytskyi ◽

Jana Čopíková ◽

...

Keyword(s):

Structural Characterization ◽

Pleurotus Ostreatus ◽

Cold Water ◽

Water Soluble ◽

Hot Water ◽

Biologically Active ◽

Alkaline Extract ◽

A Cell ◽

Oyster Mushrooms

Oyster mushrooms are an interesting source of biologically active glucans and other polysaccharides. This work is devoted to the isolation and structural characterization of polysaccharides from basidiocarps of the cultivated oyster mushroom, Pleurotus ostreatus. Five polysaccharidic fractions were obtained by subsequent extraction with cold water, hot water and two subsequent extractions with 1 m sodium hydroxide. Branched partially methoxylated mannogalactan and slightly branched (1→6)-β-d-glucan predominated in cold- and hot-water-soluble fractions, respectively. Alternatively, these polysaccharides were obtained by only hot water extraction and subsequent two-stage chromatographic separation. The alkali-soluble parts originating from the first alkali extraction were then fractionated by dissolution in dimethyl sulfoxide (DMSO). The polysaccharide insoluble in DMSO was identified as linear (1→3)-α-d-glucan, while branched (1→3)(1→6)-β-d-glucans were found to be soluble in DMSO. The second alkaline extract contained the mentioned branched β-d-glucan together with some proteins. Finally, the alkali insoluble part was a cell wall complex of chitin and β-d-glucans.

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