Antioxidants, Free Radicals, Storage Proteins, and Proteolytic Activities in Wheat (Triticum durum) Seeds during Accelerated Aging

The test of accelerated aging of seeds (AAS) was applied on 7 genotypes of durum wheat, and the morphophysiological parameters of seeds and seedlings, such as dynamics of seed germination, length of seedling roots, fresh and dry biomass of seedlings, were investigated. The genotypic features of collection samples of durum wheat were identified after exposure to stress factors (high temperature and humidity). Such a grouping of genotypes according to their potential ability to preserve the viability of seeds is an important complex characteristic of collection samples when they are placed for long-term storage in a plant gene bank.

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Vitamin C in Acerola and Red Plum Extracts: Quantification via HPLC, in Vitro Antioxidant Activity, and Stability of their Gel and Emulsion Formulations

Journal of AOAC International ◽

10.5740/jaoacint.18-0008 ◽

2018 ◽

Vol 101 (5) ◽

pp. 1461-1465 ◽

Cited By ~ 1

Author(s):

Letícia Caramori Cefali ◽

Ludmila de Oliveira Maia ◽

Rebeca Stahlschimidt ◽

Janaína Artem Ataide ◽

Elias Basile Tambourgi ◽

...

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Vitamin C ◽

Antioxidant Activities ◽

Accelerated Aging ◽

Stability Study ◽

Transparent Glass ◽

Glass Containers ◽

In Vitro Antioxidant Activity

Abstract Background: The fruits acerola and red plum are known to be good sources of antioxidants, particularly vitamin C. Antioxidants are compounds that protect organisms from biomolecular damage, such as accelerated aging, caused by free radicals. Objective: The objective of this study was to extract vitamin C from acerola and red plum, incorporate these extracts into different topical formulations, and evaluate the physicochemical stabilities of these formulations under stress conditions. Methods: Vitamin C was extracted from acerola and red plum via dynamic maceration for 2 h at 50 ± 2°C and was quantified via HPLC. In vitro antioxidant activities were evaluated using DPPH assays. The extracts were then incorporated into emulsion and gel formulations in two types of packaging, and stability studies were carried out. Results: Red plum and acerola extracts were orange and red and contained vitamin C concentrations of 2732.70 ± 93.01 mg/100 g and 2.60 ± 1.2 mg/100 g, respectively. In vitro antioxidant activity resulted in over 90.0% inhibition of free radicals at 0.01 mL/mL acerola extract and 0.1 mL/mL red plum extract. In the stability study, pH values decreased for both acerola formulations when stored in the oven or in transparent glass containers. Formulations containing red plum extract were stable under all conditions. Acerola extracts contained a higher concentration of vitamin C than red plum extracts. Both extracts possessed antioxidant activity, although the acerola-based formulation was unstable when stored at high temperatures or in transparent glass containers. Highlights: Extracts from red plum and acerola contained vitamin C; antioxidant activity of the extracts resulted in over 90.0% inhibition of free radicals. Formulations containing red plum were stable under all tested conditions, and formulations containing acerola were unstable when stored in the oven or in transparent glass containers.

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Physiological quality, content and activity of antioxidants in soybean seeds artificially aged

Revista Brasileira de Sementes ◽

10.1590/s0101-31222012000300006 ◽

2012 ◽

Vol 34 (3) ◽

pp. 397-407 ◽

Cited By ~ 3

Author(s):

Marizangela Rizzatti Ávila ◽

Alessandro de Lucca e Braccini ◽

Cristina Giatti Marques de Souza ◽

José Marcos Gontijo Mandarino ◽

Gabriel Loli Bazo ◽

...

Keyword(s):

Free Radicals ◽

Phenolic Compounds ◽

Accelerated Aging ◽

Positive Behavior ◽

Soybean Seeds ◽

Aging Period ◽

Antioxidant Agents ◽

Physiological Quality ◽

Aged Seeds

The objective of this study was to evaluate physiological quality, content, and activity of antioxidants, in soybean seeds subjected to accelerated aging during different periods. Seeds of cultivars BRS 258, BRS 262 and BRS 268, subjected to accelerated aging during 12, 24, 36 and 48 hours and non-aged seeds were used. After each aging period, the seed were evaluated by tests of: germination; first count and tetrazolium. The total of phenolic compounds, total flavonoides, total of isoflavones, and activity for eliminating ABTS●+ radicals were quantified. There were differences among cultivars according to vigor and viability only after seeds were aged. Cultivars BRS 158 and BRS 268 have shown better seed physiological quality in each aging period; however, not presenting higher amounts of isoflavones and efficiency in removing free radicals. For all cultivars, the values for total of phenolic compounds, as well as total of flavonoids have shown quadratic positive behavior; the values for isoflavones remained constant and the vigor and viability showed contrary trend to activity of antioxidant agents.

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Yield and quality of soybean seeds inoculated with Bacillus subtilis strains

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v24n1p65-71 ◽

2020 ◽

Vol 24 (1) ◽

pp. 65-71 ◽

Cited By ~ 1

Author(s):

Tauan R. Tavanti ◽

Renan F. R. Tavanti ◽

Fernando S. Galindo ◽

Isadora Simões ◽

Larissa S. Dameto ◽

...

Keyword(s):

Bacillus Subtilis ◽

Seed Quality ◽

Storage Proteins ◽

Seedling Emergence ◽

Accelerated Aging ◽

Sprinkler Irrigation ◽

Seed Vigor ◽

Soybean Cultivars ◽

Yield And Quality

ABSTRACT The use of growth-promoting bacteria can increase the yield and quality of seeds of several annual crops, such as soybean. Based on that, the present study aimed to evaluate the effects of the inoculation of Bacillus subtilis strains on seed treatment, evaluating the yield and quality of seeds of two soybean cultivars. The experiment was conducted in a conventional system, in an Oxisol of clayey texture, in a crop under sprinkler irrigation. The experimental design was randomized blocks, arranged in split plots with four repetitions. Treatments were composed, in the plots, of two soybean cultivars: M7110 IPRO and Brasmax Desafio RR. Within the levels of subplots, the treatments consisted of inoculation and doses of inoculant: Bacillus subtilis Pant001 strain at doses of 0, 2, 3, 4 and 5 mL kg-1, and QST713 strain at the recommended dose of 2 mL kg-1. Inoculation with the strains Pant001 and QST713 increased the yield of both soybean cultivars tested, besides improving seed quality due to the increase in the concentration of total storage proteins, seedling emergence percentage and seed vigor by the accelerated aging test.

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Accelerated aging of ipê seeds under controlled conditions of storage

Revista Brasileira de Sementes ◽

10.1590/s0101-31222012000200009 ◽

2012 ◽

Vol 34 (2) ◽

pp. 247-254 ◽

Cited By ~ 3

Author(s):

Marília Shibata ◽

Cileide Maria Medeiros Coelho ◽

Luciana Magda de Oliveira ◽

Cristhyane Garcia

Keyword(s):

Aging Process ◽

Storage Proteins ◽

Protein Extraction ◽

Accelerated Aging ◽

Polyacrylamide Gel ◽

Germination Test ◽

Beneficial Effects ◽

Extraction Buffer ◽

Sds Page ◽

Cold Chamber

This research was aimed at studying effects of storage and accelerated aging on germination and profile of storage proteins in Handroanthus albus seeds. These were stored into a cold chamber (± 8 ºC; RH ± 40%) and after periods of 0, 3, 6, 9, and 12 months of storage, were subjected to accelerated aging for 0, 24, 48, 72, and 96 hours. Relationships between germination and proteins profile were assessed. Germination test was performed at 25 ºC, under constant light. For protein extraction, 125 mg of seeds were macerated in 2 mL of extraction buffer (1M Tris-HCl; pH 8.8) and applied to SDS-PAGE polyacrylamide gel at 80 V .15 h-1. Twelve month storage, combined with 72 hours accelerated aging have increased germination in approximately 65% when compared to non-aged seeds or to seeds with 24 h of accelerated aging. Besides beneficial effects, degradation and synthesis of different proteins were observed. It was concluded that germination of Handroanthus albus seeds, when not subjected to accelerated aging, is favored by storage in cold chamber during three to six months, or from nine to 12 months when subjected to accelerated aging process. Storage proteins may be associated to those increases, and hence further studies are needed.

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