scholarly journals Identification of Cytotoxic Constituents of Narthecium ossifragum Using Bioassay-Guided Fractionation

2008 ◽  
Vol 56 (6) ◽  
pp. 2297-2297
Author(s):  
Silvio Uhlig ◽  
Helene Wisløff ◽  
Dirk Petersen
Keyword(s):  
Narthecium Ossifragum ◽  
Bioassay Guided Fractionation

Bioassay-guided fractionation of Eupatorium Perfoliatum L. towards anti-Influenza A-activity leads to dicaffeoyl quinic acids

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Derksen ◽  
J Sendker ◽  
W Hafezi ◽  
C Nauert ◽  
J Kühn ◽  
...  
Keyword(s):  
Influenza A ◽  
Bioassay Guided Fractionation ◽  
Eupatorium Perfoliatum

scholarly journals Narthecium ossifragum Associated Photosensitization in Sheep in The Faroe Islands

1995 ◽  
Vol 36 (2) ◽  
pp. 277-278
Author(s):  
Arne Flåøyen ◽  
Johannes Jóhansen ◽  
Jústines Olsen
Keyword(s):  
Faroe Islands ◽  
Narthecium Ossifragum

scholarly journals Antimicrobial metabolite profiling of Nigrospora sphaerica from Adiantum philippense L.

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Kolathuru Puttamadaiah Ramesha ◽  
Nagabhushana Chandra Mohana ◽  
Bettadapura Rameshgowda Nuthan ◽  
Devaraju Rakshith ◽  
Sreedharamurthy Satish
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Xanthomonas Campestris ◽  
Fungal Endophytes ◽  
Leaf Segment ◽  
Spectroscopic Techniques ◽  
Biological Potential ◽  
Bioassay Guided Fractionation ◽  
Antimicrobial Metabolite ◽  
Bacteria And Fungi

Abstract Background Endophyte bestows beneficial aspects to its inhabiting host, along with a contribution to diverse structural attributes with biological potential. In this regard, antimicrobial profiling of fungal endophytes from medicinal plant Adiantum philippense revealed bioactive Nigrospora sphaerica from the leaf segment. Chemical and biological profiling through TLC–bioautography and hyphenated spectroscopic techniques confirmed the presence of phomalactone as an antimicrobial metabolite. Results The chemical investigation of the broth extract by bioassay-guided fractionation confirmed phomalactone as a bioactive antimicrobial secondary metabolite. The antimicrobial activity of phomalactone was found to be highest against Escherichia coli by disc diffusion assay. The MIC was found to be significant against both Escherichia coli and Xanthomonas campestris in the case of bacteria and dermatophyte Candida albicans at 150 μg/ml, respectively. Conclusions Overall, the results highlighted the antimicrobial potential of phomalactone from the endophyte Nigrospora sphaerica exhibiting a broad spectrum of antimicrobial activity against human and phytopathogenic bacteria and fungi. This work is the first report regarding the antibacterial activity of phomalactone.

scholarly journals Antimicrobial Metabolites against Methicillin-Resistant Staphylococcus aureus from the Endophytic Fungus Neofusicoccum australe

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1094
Author(s):  
Melissa M. Cadelis ◽  
Soeren Geese ◽  
Benedict B. Uy ◽  
Daniel R. Mulholland ◽  
Shara J. van de Pas ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Natural Products ◽  
Structure Elucidation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Spectroscopic Methods ◽  
Methicillin Resistant ◽  
Bioassay Guided Fractionation ◽  
Antimicrobial Metabolites

Antimicrobial bioassay-guided fractionation of the endophytic fungi Neofusicoccum australe led to the isolation of a new unsymmetrical naphthoquinone dimer, neofusnaphthoquinone B (1), along with four known natural products (2–5). Structure elucidation was conducted by nuclear magnetic resonance (NMR) spectroscopic methods, and the antimicrobial activity of all the natural products was investigated, revealing 1 to be moderately active towards methicillin-resistant Staphylococcus aureus (MRSA) with a minimum inhibitory concentration (MIC) of 16 µg/mL.

Bioassay-guided fractionation of antifertility components of castorbean (Ricinus communisL.) seed extracts

2007 ◽  
Vol 21 (11) ◽  
pp. 982-989 ◽  
Author(s):  
Xiaoxue Zhang ◽  
Feng Han ◽  
Ping Gao ◽  
Dong Yu ◽  
Shigui Liu
Keyword(s):  
Bioassay Guided Fractionation ◽  
Seed Extracts

scholarly journals Extratos de Piper marginatume Azadirachta indica no controle de Colletotrichum scovillei em pimentão

2014 ◽  
Vol 49 (2) ◽  
pp. 88-94 ◽  
Author(s):  
Emmanuelle Rodrigues Araújo ◽  
Wolfgang Harand ◽  
Iwanne Coelho Lima ◽  
Fernanda Carolina Ribeiro Dias ◽  
Adelmo Adriane Duarte de Santana ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Bioassay Guided Fractionation ◽  
Colletotrichum Scovillei

O objetivo deste trabalho foi avaliar o efeito de extratos de capeba (Piper marginatum) e nim (Azadirachta indica) sobre o fungo Colletotrichum scovillei e determinar o componente mais ativo no controle pós-colheita da antracnose em pimentão. A atividade de extratos metanólicos das folhas (P.marginatum e A. indica) e das sementes (A. indica) a 0, 125, 250, 500, 1.000 e 2.000 ppm, na inibição do crescimento micelial de C. scovillei in vitro foram avaliadas. O extrato metanólico de folhas de P.marginatum foi o mais ativo e, consequentemente, foi submetido ao fracionamento biomonitorado ("bioassay-guided fractionation"). Esse processo rendeu 10 frações majoritárias, obtidas por cromatografia líquida de alta performance, das quais a fração à concentração de 1,5 ppm inibiu o desenvolvimento de C. scovillei de forma mais eficiente do que o fungicida mancozeb.

scholarly journals Lignans from Bursera fagaroides Affect In Vivo Cell Behavior by Disturbing the Tubulin Cytoskeleton in Zebrafish Embryos

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 8 ◽  
Author(s):  
Mayra Antúnez-Mojica ◽  
Andrés Rojas-Sepúlveda ◽  
Mario Mendieta-Serrano ◽  
Leticia Gonzalez-Maya ◽  
Silvia Marquina ◽  
...  
Keyword(s):  
Cell Migration ◽  
Biological Effects ◽  
In Vitro Models ◽  
In Vivo Model ◽  
Zebrafish Embryos ◽  
Microtubule Cytoskeleton ◽  
Zebrafish Model ◽  
Bioassay Guided Fractionation

By using a zebrafish embryo model to guide the chromatographic fractionation of antimitotic secondary metabolites, seven podophyllotoxin-type lignans were isolated from a hydroalcoholic extract obtained from the steam bark of Bursera fagaroides. The compounds were identified as podophyllotoxin (1), β-peltatin-A-methylether (2), 5′-desmethoxy-β-peltatin-A-methylether (3), desmethoxy-yatein (4), desoxypodophyllotoxin (5), burseranin (6), and acetyl podophyllotoxin (7). The biological effects on mitosis, cell migration, and microtubule cytoskeleton remodeling of lignans 1–7 were further evaluated in zebrafish embryos by whole-mount immunolocalization of the mitotic marker phospho-histone H3 and by a tubulin antibody. We found that lignans 1, 2, 4, and 7 induced mitotic arrest, delayed cell migration, and disrupted the microtubule cytoskeleton in zebrafish embryos. Furthermore, microtubule cytoskeleton destabilization was observed also in PC3 cells, except for 7. Therefore, these results demonstrate that the cytotoxic activity of 1, 2, and 4 is mediated by their microtubule-destabilizing activity. In general, the in vivo and in vitro models here used displayed equivalent mitotic effects, which allows us to conclude that the zebrafish model can be a fast and cheap in vivo model that can be used to identify antimitotic natural products through bioassay-guided fractionation.

scholarly journals Constituents of Talisia nervosa with Potential Utility against Metabolic Syndrome

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400 ◽  
Author(s):  
Yelkaira Vásquez ◽  
Jianping Zhao ◽  
Shabana I. Khan ◽  
Mahabir P. Gupta ◽  
Ikhlas A. Khan
Keyword(s):  
Metabolic Syndrome ◽  
Ethanolic Extract ◽  
Fold Increase ◽  
No Production ◽  
Methyl Gallate ◽  
Potent Effect ◽  
Cellular Assays ◽  
The Metabolic Syndrome ◽  
Isolation And Characterization ◽  
Bioassay Guided Fractionation

This study is focused on the isolation and characterization of bioactive secondary metabolites from the ethanolic extract of stems of the Panamanian plant Talisia nervosa Radlk, through a series of target-based cellular assays related to the metabolic syndrome (MetS): a combination of type 2 Diabetes Mellitus (T2DM), hypercholesterolemia, inflammation, and obesity. Bioassay guided fractionation allowed the isolation of four known compounds: (-)-catechin (1), methyl gallate (2), ethyl gallate (3), and ß-D-glucopyranose,1,4,6-tris(3,4,5-trihydroxybenzoate) (4). This is the first report of (-)-catechin (1) and ß-D-glucopyranose,1,4,6-tris (3,4,5-trihydroxybenzoate) (4) from T. nervosa. Among the isolates, 1 activated PPARγ, but had no effect on PPARα. Compounds 2 -4 activated PPARα, PPARγ and LXR. Interestingly, 2 was stronger than 3 towards all three targets. Methyl gallate (2) showed the most potent effect toward both PPARα and PPARγ with an increase of 3.0 and 13-fold, respectively, while 4 was most potent in activating LXR with a fold induction of 5.3 at concentrations of 100 μg/mL. The nitric oxide (NO) production was reduced by compounds 2 and 3 with IC50 values of 7.0 and 7.5 μg/mL, respectively. ß-D-glucopyranose,1,4,6-tris (3,4,5-trihydroxybenzoate) 4) did not cause a significant increase in adipogenesis despite its strong PPARγ agonistic action (8 6-fold increase) and may represent a good candidate for the treatment of MetS without the undesirable side effect of weight gain.

Identification of Cytotoxic Constituents ofNarthecium ossifragumUsing Bioassay-Guided Fractionation

2007 ◽  
Vol 55 (15) ◽  
pp. 6018-6026 ◽  
Author(s):  
Silvio Uhlig ◽  
Helene Wisløff ◽  
Dirk Petersen
Keyword(s):  
Bioassay Guided Fractionation
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