scholarly journals Effect of Molecular Structure of Cationic Surfactants on Biophysical Interactions of Surfactant-Modified Nanoparticles with a Model Membrane and Cellular Uptake

Langmuir ◽  
2009 ◽  
Vol 25 (4) ◽  
pp. 2369-2377 ◽  
Author(s):  
Chiranjeevi Peetla ◽  
Vinod Labhasetwar
Keyword(s):  
Molecular Structure ◽  
Cellular Uptake ◽  
Cationic Surfactants ◽  
Model Membrane ◽  
Biophysical Interactions

Surface activity of cationic surfactants, influence of molecular structure

2010 ◽  
Vol 365 (1-3) ◽  
pp. 215-221 ◽  
Author(s):  
Grażyna Para ◽  
Agnieszka Hamerska-Dudra ◽  
Kazimiera Anna Wilk ◽  
Piotr Warszyński
Keyword(s):  
Molecular Structure ◽  
Surface Activity ◽  
Cationic Surfactants

Novel cationic surfactants with cleavable carbamate fragment: Tunable morphological behavior, solubilization of hydrophobic drugs and cellular uptake study

2020 ◽  
Vol 318 ◽  
pp. 113894 ◽  
Author(s):  
Rushana A. Kushnazarova ◽  
Alla B. Mirgorodskaya ◽  
Svetlana S. Lukashenko ◽  
Alexandra D. Voloshina ◽  
Anastasiia S. Sapunova ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Cationic Surfactants ◽  
Hydrophobic Drugs ◽  
Uptake Study ◽  
Morphological Behavior

THE EFFECT OF RESIDUAL POLY(VINYL ALCOHOL) ON BIOPHYSICAL INTERACTION OF NANOPARTICLES WITH ENDOTHELIAL CELL MODEL MEMBRANE

2011 ◽  
Vol 10 (04n05) ◽  
pp. 539-545 ◽  
Author(s):  
RADHIKA BHAVE ◽  
CHIRANJEEVI PEETLA ◽  
VINOD LABHASETWAR
Keyword(s):  
Endothelial Cell ◽  
Gene Delivery ◽  
Surface Pressure ◽  
Vinyl Alcohol ◽  
Membrane Lipids ◽  
Cell Model ◽  
Interfacial Properties ◽  
Model Membrane ◽  
Poly Vinyl Alcohol ◽  
Biophysical Interactions

Poly(vinyl alcohol) (PVA) is commonly used as an emulsifier in the formulation of nanoparticles (NPs) comprising poly DL-lactide co-glycolide and polylactide-based biodegradable polymers for drug and gene delivery applications. A fraction of PVA remains associated with the NPs at the interface despite their repeated washing (residual PVA). We hypothesize that this residual PVA influences the interfacial properties of NPs and hence their biophysical interactions with membrane lipids. In this study, we formulated NPs using PVA of different molecular weights to determine the effects of the residual PVA on biophysical interactions of the formulated NPs with the endothelial cell model membrane using a Langmuir balance. Despite similar physical properties (particle size and zeta potential), NPs formulated with different PVA demonstrated significant variations in their intrinsic surface properties and biophysical interactions with the model membrane. This was evident from the difference in the surface pressure–area (π–A) isotherms prepared in the presence of different formulations of NPs and the change in surface pressure of the model membrane following interaction with NPs. The variation in the biophysical properties was observed even with the NPs formulated using the same molecular weight PVA but obtained from different lots. Since the interfacial properties of NPs can significantly influence NP interactions with cells and tissue, their biophysical characterization could prove to be an important parameter not only to obtain consistent results with NPs but also to optimize their properties for drug/gene delivery applications.

Molecular Structure of the Outermost Cell Wall Component of Spirillum Serpens

1977 ◽  
Vol 35 ◽  
pp. 342-343
Author(s):  
Wah Chiu ◽  
David Grano
Keyword(s):  
Molecular Structure ◽  
Cell Wall ◽  
Outer Membrane ◽  
Gel Electrophoresis ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Cell Wall Component ◽  
Protein Components ◽  
Exposure Technique ◽  
Structural Aspects

The periodic structure external to the outer membrane of Spirillum serpens VHA has been isolated by similar procedures to those used by Buckmire and Murray (1). From SDS gel electrophoresis, we have found that the isolated fragments contain several protein components, and that the crystalline structure is composed of a glycoprotein component with a molecular weight of ∽ 140,000 daltons (2). Under an electron microscopic examination, we have visualized the hexagonally-packed glycoprotein subunits, as well as the bilayer profile of the outer membrane. In this paper, we will discuss some structural aspects of the crystalline glycoproteins, based on computer-reconstructed images of the external cell wall fragments.The specimens were prepared for electron microscopy in two ways: negatively stained with 1% PTA, and maintained in a frozen-hydrated state (3). The micrographs were taken with a JEM-100B electron microscope with a field emission gun. The minimum exposure technique was essential for imaging the frozen- hydrated specimens.

Water-soluble amphiphilic ruthenium(ii) polypyridyl complexes as potential light-activated therapeutic agents

2020 ◽  
Vol 56 (65) ◽  
pp. 9332-9335
Author(s):  
Sandra Estalayo-Adrián ◽  
Salvador Blasco ◽  
Sandra A. Bright ◽  
Gavin J. McManus ◽  
Guillermo Orellana ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Therapeutic Agents ◽  
Water Soluble ◽  
Polypyridyl Complexes ◽  
Cervical Cancer Cells

Two new water-soluble amphiphilic Ru(ii) polypyridyl complexes were synthesised and their photophysical and photobiological properties evaluated; both complexes showed a rapid cellular uptake and phototoxicity against HeLa cervical cancer cells.

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