Highly Sensitive Diagnostic Assay for the Detection of Protein Biomarkers Using Microresonators and Multifunctional Nanoparticles

AbstractWe describe highly sensitive, non–invasive, label-free, electrical detection of protein biomarkers using nanoporous alumina membrane based electrochemical conductance based devices. The principle of operation of these sensors are based on electrochemical conductance varitions associated with the binding of antibody-antigen complexes to a metallic substrate.In these devices distinct pore clusters are selectively surface functionalized with specific antibodies, that are in turn are incorporated into micro scale arrays. Protein markers were routinely detected at nanomolar concentrations. This opens the potential for developing highly sensitive and selective biomarker detection assays in clinically relevant samples for diagnosis of complex disease state like vulnerable coronary plaque rupture that results in poor post surgical outcomes and other complex diseases.

Download Full-text

P2-038: A highly sensitive diagnostic assay for early diagnosis of Alzheimer's disease

Alzheimer s & Dementia ◽

10.1016/j.jalz.2009.04.347 ◽

2009 ◽

Vol 5 (4S_Part_9) ◽

pp. P273-P273

Author(s):

Susanne A. Funke ◽

Lei Wang ◽

Eva Birkmann ◽

Franziska Henke ◽

Oliver Bannach ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Early Diagnosis ◽

Diagnostic Assay ◽

Highly Sensitive

Download Full-text

Can new immunoassay techniques improve bladder cancer diagnostics With protein biomarkers?

Frontiers in Molecular Biosciences ◽

10.3389/fmolb.2020.620687 ◽

2021 ◽

Vol 7 ◽

Author(s):

Yuri M. Shlyapnikov ◽

Ekaterina A. Malakhova ◽

Andrey Z. Vinarov ◽

Andrey A. Zamyatnin ◽

Elena A. Shlyapnikova

Keyword(s):

Experimental Data ◽

Bladder Cancer ◽

Diagnostic Tests ◽

Current Knowledge ◽

Test System ◽

Cancer Diagnostics ◽

Published Data ◽

Protein Biomarkers ◽

Low Concentrations ◽

Highly Sensitive

The search for new diagnostic tests for cancer or ways to improve existing tests is primarily driven by the desire to identify the disease as early as possible. In this report, we summarize the current knowledge of the most promising diagnostic protein bladder cancer (BC) markers reported over the last decade. Unfortunately, analysis of published data suggests that a reliable, highly sensitive biomarker test-system based on ELISA for detecting BC has not yet been developed. The use of more sensitive assays to detect ultra-low concentrations of biomarkers not available for ELISA, could be very beneficial. Based on the literature and pilot experimental data, we conclude that a highly sensitive immunoassay using microarrays and magnetic labels, could be an effective and cheap technique suitable for the detection of diagnostically relevant BC biomarkers.

Download Full-text

A HIGHLY SENSITIVE DIAGNOSTIC ASSAY FOR ALZHEIMERS DISEASE

10.1240/sav_gbm_2007_h_001947 ◽

2007 ◽

Vol 2007 (Fall) ◽

Author(s):

Susanne Aileen Funke ◽

Eva Birkmann ◽

Franziska Henke ◽

Detlev Riesner ◽

Dieter Willbold

Keyword(s):

Diagnostic Assay ◽

Highly Sensitive

Download Full-text

BIOSENSING AND MEDICAL DIAGNOSTICS BASED ON ULTRA SENSITIVE OPTICAL AND MAGNETIC MEASUREMENTS

BIOTECHNOLOGY: STATE OF THE ART AND PERSPECTIVES ◽

10.37747/2312-640x-2021-19-238-240 ◽

2021 ◽

Vol 1 (19) ◽

pp. 238-240

Author(s):

A.V. Orlov ◽

V.A. Bragina ◽

N.V. Gyteneva ◽

M.P. Nikitin ◽

B.G. Gorshkov

Keyword(s):

Food Safety ◽

Magnetic Measurements ◽

Detection Limits ◽

Medical Diagnostics ◽

Protein Biomarkers ◽

Safety Control ◽

Analytical Applications ◽

Highly Sensitive

Highly sensitive methods of optical and magnetic measurements have been developed and tested for analytical applications, medical diagnostics, food safety control, etc. The achieved detection limits are unique for express assays: DNA - 6 × 10-19 mol (without amplification), haptens - 16 fg/ml, protein biomarkers - 4 pg/ml.

Download Full-text