Quantitation and Kinetics of CD51 Surface Receptor Expression: Implications for Targeted Delivery

Hematopoiesis has been considered hierarchical in nature, but recent data suggest that the system is not hierarchical and is, in fact, quite functionally plastic. Existing data indicate that engraftment and progenitor phenotypes vary inversely with cell cycle transit and that gene expression also varies widely. These observations suggest that there is no progenitor/stem cell hierarchy, but rather a reversible continuum. This may, in turn, be dependent on shifting chromatin and gene expression with cell cycle transit. If the phenotype of these primitive marrow cells changes from engraftable stem cell to progenitor and back to engraftable stem cell with cycle transit, then this suggests that the identity of the engraftable stem cell may be partially masked in nonsynchronized marrow cell populations. A general model indicates a marrow cell that can continually change its surface receptor expression and thus responds to external stimuli differently at different points in the cell cycle.

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Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

International Journal of Molecular Sciences ◽

10.3390/ijms19102912 ◽

2018 ◽

Vol 19 (10) ◽

pp. 2912 ◽

Cited By ~ 5

Author(s):

Girdhari Rijal ◽

Jing Wang ◽

Ilhan Yu ◽

David Gang ◽

Roland Chen ◽

...

Keyword(s):

Extracellular Matrix ◽

Human Mammary Epithelial Cell ◽

Tumor Formation ◽

Receptor Expression ◽

Cell Surface Receptor ◽

Breast Diseases ◽

Porous Scaffolds ◽

Ecm Proteins ◽

Surface Receptor

Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.

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Modeling the Coalescence Kinetics of Cell Surface Receptor Clusters

Biophysical Journal ◽

10.1016/j.bpj.2010.12.1603 ◽

2011 ◽

Vol 100 (3) ◽

pp. 254a

Author(s):

Kathrin Spendier ◽

James L. Thomas ◽

Vasudev M. Kenkre

Keyword(s):

Cell Surface ◽

Cell Surface Receptor ◽

Surface Receptor ◽

Receptor Clusters ◽

Kinetics Of

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Drebrin 1 in dendritic cells regulates phagocytosis and cell surface receptor expression through recycling for efficient antigen presentation

Immunology ◽

10.1111/imm.13010 ◽

2018 ◽

Vol 156 (2) ◽

pp. 136-146 ◽

Cited By ~ 4

Author(s):

Diana M. Elizondo ◽

Temesgen E. Andargie ◽

Naomi L. Haddock ◽

Thomas A. Boddie ◽

Michael W. Lipscomb

Keyword(s):

Dendritic Cells ◽

Cell Surface ◽

Antigen Presentation ◽

Receptor Expression ◽

Cell Surface Receptor ◽

Surface Receptor

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Cytoplasts and Mo1-deficient neutrophils pretreated with plasma and LPS induce lung injury

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1989.256.3.h751 ◽

1989 ◽

Vol 256 (3) ◽

pp. H751-H759

Author(s):

M. L. Wencel ◽

M. L. Morganroth ◽

S. O. Schoeneich ◽

D. E. Gannon ◽

J. Varani ◽

...

Keyword(s):

Lung Injury ◽

Lung Parenchyma ◽

Fixed Number ◽

Receptor Expression ◽

Human Neutrophils ◽

Neutrophil Adhesion ◽

Cell Monolayers ◽

Induce Lung Injury ◽

Surface Receptor ◽

Normal Human

We hypothesized that neutrophil adhesion and lung injury could occur independent of the surface receptor glycoprotein, Mo1 (C3bi receptor). We investigated whether preincubation of human neutrophil-derived cytoplasts (cell fragments that lack nuclei and granules and have a fixed number of surface Mo1 receptors) with plasma and lipopolysaccharide (LPS) would augment the cytoplasts' ability to cause lung injury when activated. We also investigated whether preincubating normal human neutrophils treated with anti-Mo1 antibody with plasma and LPS would increase the neutrophils' ability to adhere and cause lung injury. Human neutrophils infused into isolated salt-perfused rat lungs subsequently stimulated with phorbol 12-myristate 13-acetate (PMA) resulted in lung injury as assessed by the accumulation of 125I-bovine serum albumin in the lung parenchyma. The infusion of cytoplasts resulted in significantly less injury. Cytoplasts preincubated in 20% human plasma and LPS caused an increase in lung injury. Similarly, neutrophils treated with plasma, LPS, and anti-Mo1 antibody or neutrophils congenitally deficient in the Mo1 surface receptor and treated with plasma and LPS augmented lung injury. Plasma and LPS preincubation also increased anti-Mo1 antibody-treated neutrophil adhesion to endothelial cell monolayers after activation by PMA. Thus, plasma and LPS increase adhesion and lung injury caused by neutrophils or neutrophil fragments that share defects in Mo1 receptor expression.

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Activation-induced changes in platelet surface receptor expression and the contribution of the large-platelet subpopulation to activation

Research and Practice in Thrombosis and Haemostasis ◽

10.1002/rth2.12303 ◽

2020 ◽

Vol 4 (2) ◽

pp. 285-297

Author(s):

Masaaki Moroi ◽

Richard W. Farndale ◽

Stephanie M. Jung

Keyword(s):

Receptor Expression ◽

Platelet Surface ◽

Large Platelet ◽

Surface Receptor ◽

Induced Changes

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Selective inhibition of the growth of human erythroid bursts by monoclonal antibodies against transferrin or the transferrin receptor

Blood ◽

10.1182/blood.v67.6.1631.1631 ◽

1986 ◽

Vol 67 (6) ◽

pp. 1631-1638 ◽

Cited By ~ 16

Author(s):

KM Shannon ◽

JW Larrick ◽

SA Fulcher ◽

KB Burck ◽

J Pacely ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Transferrin Receptor ◽

Thymidine Incorporation ◽

Selective Inhibition ◽

Receptor Expression ◽

Cell Surface Receptor ◽

Transferrin Receptors ◽

Human Erythropoietin ◽

Surface Receptor ◽

Transferrin Receptor Expression

Abstract The relative requirements of colonies derived from erythroid (BFU-E) and myeloid (CFU-c) progenitors for transferrin were examined using monoclonal antibodies directed against the transferrin molecule (TF-6) or its cell surface receptor (TFR-A12, TFR1–2B). Growth of erythroid bursts was profoundly reduced at concentrations of all three antibodies that had no effect on CFU-c-derived colonies. When TFR1–2B was layered over cultures established one to seven days previously, further burst development was inhibited, and degeneration of early erythroid colonies was observed. Addition of erythropoietin augmented transferrin receptor expression on cells harvested after 1 to 2 weeks in culture and analyzed by flow cytometry. Recombinant human erythropoietin gave results comparable to those obtained in experiments using human urinary erythropoietin. Analysis of erythroblasts plucked directly from culture plates confirmed the presence of transferrin receptors on BFU-E-derived colonies. Thymidine incorporation was maximal early in the second week of culture and coincided with high transferrin receptor expression. These data demonstrate that transferrin must be available into the second week of culture to support the growth and differentiation of BFU- E-derived erythroid bursts, that the generation of erythroid colonies from BFU-E is more dependent on transferrin than myeloid colony formation from CFU-c, and that erythropoietin modulates the expression of transferrin receptors on growing bursts.

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