High Intake of Magnesium in Relation to the Ruminal Transmural Potential Difference and Magnesium Absorption in Wethers

2004 ◽  
Vol 74 (3) ◽  
pp. 217-222
Author(s):  
Jittakhot ◽  
Schonewille ◽  
Haddad ◽  
Wouterse ◽  
Yuangklang ◽  
...  

High potassium (K) intakes are known to decrease magnesium (Mg) absorption in ruminants by increasing the transmural potential difference (PDt, serosal side = positive). High Mg intakes are known to increase the amount of Mg absorbed, which may be explained by increasing the ruminal Mg concentration, but an effect on the PDt cannot be excluded. The objective of this study was to determine whether or not Mg intake affects the PDt. In a 3x3 Latin square design, six ruminally fistulated wethers were fed a low-Mg, low-K ration (3.88 g Mg/kg dry matter (DM); 30.7 g K/kg DM), a high-Mg, low K-ration (16.79 g Mg/kg DM; 30.7 g K/kg DM), and a low-Mg ration high-K (3.88 g Mg/kg DM or 62.1 g K/kg DM). When compared with the low-Mg, low-K ration, the high-Mg, low-K ration raised the absolute apparent Mg absorption (g/day) by 421% and the low-Mg, high-K ration decreased it by 20%. The intake of extra K produced a significant increase in the PDt. The intake of extra Mg did not change the PDt across the rumen wall but produced a significant increase of the ruminal Mg concentrations. On the basis of the individual values for three rations, the mean post feeding ruminal Mg concentrations were found to be unrelated to the PDt (Pearson's r = –0.329, p = 0.183, n = 18). Thus, it is concluded that the observed increase in Mg absorption after a high Mg intake can be explained by an increase in the ruminal Mg concentration rather than by a change in PDt.

1960 ◽  
Vol 55 (2) ◽  
pp. 251-260 ◽  
Author(s):  
M. E. Castle ◽  
W. Holmes

1. A small-scale plot experiment which had been conducted from 1947 to 1952 to study the effect of massive dressings of fertilizer nitrogen, with and without phosphate and potash, on the yield and quality of a rye-grass dominant sward was continued from 1953 to 1958 with two changes in the mineral treatments in 1953. A 4 × 4 Graeco-Latin square was used.The nitrogen treatments applied each year were: (1) no nitrogenous fertilizer, (2) 260 lb. nitrogen, (3) 520 lb. nitrogen and (4) 416 lb. nitrogen per acre per year.The mineral treatments were: (A) no mineral fertilizer, (B) 336–504 lb. K2O per acre per year depending on nitrogen treatment, (C) 101–202 lb. K2O and 81–101 lb. P2O5 per acre per year depending on nitrogen treatment, and (D) no mineral fertilizer after 1953, although K2O and P2O5 had been applied during the previous 6-year period.All the fertilizers were applied in four or five equal dressings each year, one for each cut.Treatments 1 and 4 were cut four times and treatments 2 and 3 five times per year.2. Applications of phosphate did not affect the yields of herbage dry matter or crude protein, but yields were severely depressed in the absence of potash. With adequate potash the yields under all nitrogen treatments were maintained for the 6-year period and were at the same level as in the previous 6-year period. The mean yields of dry matter for the 6-year period when potash was applied were 5200, 8420, 10,180 and 9700 lb. per acre per year for treatments, 1, 2, 3 and 4. Without potash the corresponding mean yields were 5460, 6410, 6210 and 6880 lb. Average crude protein yields were 760, 1360, 1940 and 1660 lb. per acre per year with potash and 780, 1100, 1220 and 13201b. without potash.3. The weighted mean contents of crude protein for the 6-year period averaged over all the mineral treatments were 14·4, 16·7, 19·4 and 18·2% for nitrogen treatments 1, 2, 3 and 4. The mean crudeprotein contents of treatments with and without potash were 16·7 and 17·6%, respectively.4. The average response to nitrogen was 15·0, 10·9 and 12·4 lb. dry matter per 1 lb. of nitrogen applied on treatments 2, 3 and 4, respectively, compared with a no-clover sward, and 7·8, 7·3 and 7·9 lb. compared with a grass + clover sward. The recovery of nitrogen in terms of crude protein was 45, 40 and 40% for treatments 2, 3 and 4, respectively, when compared with a no-clover sward.5. The botanical composition of the plots continued to be determined by the nitrogen and potash treatments. On plots receiving ‘Nitro-Chalk’ clover was virtually non-existent, whereas on plots which received potash and no nitrogen, clover was present. With a combination of potash and nitrogen vigorous swards of timothy were found but if only nitrogen and no potash was applied, poorer grasses, such as Festuca rubra, were dominant.6. Soil analyses showed increased pH values and decreased contents of Mg with increasing applications of ‘Nitro-Chalk’. P2O5 values were low except where phosphate was being applied. The most marked change was in the K2O values which varied from 4 units on treatments A and D which received no minerals to 40 units on treatment B which received the heavy dressings of potash.7. Mineral analyses of the herbage for 1953 and 1958 are given. High K contents were found on treatments where potash was applied and this was associated with decreased Mg and Na contents.8. Some details are given of a field-scale trial on the Institute farm where high levels of nitrogen and potash have been applied for 11 and 12 years with successful results.


1999 ◽  
Vol 69 (4) ◽  
pp. 273-276
Author(s):  
Smit ◽  
Schonewille ◽  
Beynen

The hypothesis tested was that a high intake of potassium (K) interferes with cobalt (Co)-vitamin B12 metabolism in ruminants. Ouessant sheep were fed either a low- (8 g K/kg dry matter) or high-K diet (43 g K/kg dry matter) with an adequate amount of Co (124 mug Co/kg dry matter). Plasma vitamin B12 concentrations were measured. Sheep fed a low-K diet, but containing only 70 mug Co/kg dry matter, served as positive controls. Feeding the low-Co ration produced a significant decrease in plasma vitamin B12 concentrations. The high- versus low-K ration induced a significant decrease in plasma vitamin B12 during the first 6 weeks, but thereafter the values in the high-K group rose to those seen in the low-K group. The mechanism by which dietary K may interact with Co-vitamin B12 metabolism in sheep is unknown.


During the latter part of 1902 and the early months of 1903 I resolved to take as many observations of the rates of dissipation of positive and negative electric charges as possible, and to continue them over the whole 24 hours of the day, and, when opportunity offered, over longer periods. There appeared to be little information regarding the rate of dispersion during the night hours. At about the same time that these observations were being made, Nilsson was doing similar work at Upsala, and found a noticeable maximum value for atmospheric conductivity at about midnight. The observations were made on the Canterbury Plains of New Zealand, at a station about 20 feet above sea-level and about five miles due west from the sea coast. The apparatus used was Elster and Geitel’s Zerstreuungs- apparat , and the formula of reduction used was that given by them, viz:- E = 1/ t log V 0 /V- n / t ' log V' 0 /V' . In this formula E is proportional to the conductivity of the gas surrounding the instrument—for positive or negative charges, as the case may be. The constant “ n ” = ratio of capacity without cylinder ____________________________________ capacity with cylinder was determined by me to be 0·47, as the instrument was always used, with the protecting cover. The cover was always at one height above the base of the instrument, and was set so as to be as nearly co-axial with the discharging cylinder as could be judged by eye. No attempt was made to determine the actual capacity of the condenser cylinder and protecting cover, which would be a somewhat variable quantity owing- (1) to the differences on different days in attempting to cause the two to be co-axial; (2) to a certain amount of looseness in the fit of the shank of the cylinder on to its hole. The value above given for “ n "is the mean of several deter­minations made with different settings of the cover and cylinder. The individual values of “ n ” varied over about 0.03.


2020 ◽  
Vol 158 (1-2) ◽  
pp. 150-158 ◽  
Author(s):  
B. C. Silva ◽  
M. V. C. Pacheco ◽  
L. A. Godoi ◽  
F. A. S. Silva ◽  
D. Zanetti ◽  
...  

AbstractAn experiment was conducted to evaluate: (1) the effects of ensiling maize or sorghum grains after reconstitution on readily soluble fraction (a), potentially degradable fraction in the rumen (b) and rate constant for degradation of b (c) of dry matter (DM), organic matter (OM) and starch (STA); and (2) an appropriate incubation time for in situ or in vitro procedures to estimate in vivo digestibility. Four rumen-cannulated Nellore bulls (body weight = 262 ± 19.6 kg) distributed in a 4 × 4 Latin square were used. Diets were based on dry ground maize (DGM); or dry ground sorghum (DGS); or reconstituted ground maize silage; or reconstituted ground sorghum silage. In vitro and in situ incubations of the individual grains and diets were simultaneously performed with in vivo digestibility. In general, reconstituted grains and diets based on reconstituted grains presented greater (P < 0.05) fraction a and lower (P < 0.05) fraction b of DM, OM and STA compared to dry grains and diets based on dry grain. However, the magnitude of response of the reconstitution and ensiling process on DM and OM degradability parameter was greater for maize than that for sorghum. Moreover, no differences (P > 0.05) were observed between DGM- and DGS-based diets for c estimates. The results suggest that the reconstitution process promotes grains protein matrix breakdown increasing STA availability. The incubation times required for in vivo digestibility estimations of DM, OM and STA are 24 h for in situ and 36 h for in vitro procedures.


2018 ◽  
Vol 11 (7) ◽  
pp. 4059-4072 ◽  
Author(s):  
Sergio Fabián León-Luis ◽  
Alberto Redondas ◽  
Virgilio Carreño ◽  
Javier López-Solano ◽  
Alberto Berjón ◽  
...  

Abstract. Total ozone column measurements can be made using Brewer spectrophotometers, which are calibrated periodically in intercomparison campaigns with respect to a reference instrument. In 2003, the Regional Brewer Calibration Centre for Europe (RBCC-E) was established at the Izaña Atmospheric Research Center (Canary Islands, Spain), and since 2011 the RBCC-E has transferred its calibration based on the Langley method using travelling standard(s) that are wholly and independently calibrated at Izaña. This work is focused on reporting the consistency of the measurements of the RBCC-E triad (Brewer instruments #157, #183 and #185) made at the Izaña Atmospheric Observatory during the period 2005–2016. In order to study the long-term precision of the RBCC-E triad, it must be taken into account that each Brewer takes a large number of measurements every day and, hence, it becomes necessary to calculate a representative value of all of them. This value was calculated from two different methods previously used to study the long-term behaviour of the world reference triad (Toronto triad) and Arosa triad. Applying their procedures to the data from the RBCC-E triad allows the comparison of the three instruments. In daily averages, applying the procedure used for the world reference triad, the RBCC-E triad presents a relative standard deviation equal to σ = 0.41 %, which is calculated as the mean of the individual values for each Brewer (σ157 = 0.362 %, σ183 = 0.453 % and σ185 = 0.428 %). Alternatively, using the procedure used to analyse the Arosa triad, the RBCC-E presents a relative standard deviation of about σ = 0.5 %. In monthly averages, the method used for the data from the world reference triad gives a relative standard deviation mean equal to σ = 0.3 % (σ157 = 0.33 %, σ183 = 0.34 % and σ185 = 0.23 %). However, the procedure of the Arosa triad gives monthly values of σ = 0.5 %. In this work, two ozone data sets are analysed: the first includes all the ozone measurements available, while the second only includes the simultaneous measurements of all three instruments. Furthermore, this paper also describes the Langley method used to determine the extraterrestrial constant (ETC) for the RBCC-E triad, the necessary first step toward accurate ozone calculation. Finally, the short-term or intraday consistency is also studied to identify the effect of the solar zenith angle on the precision of the RBCC-E triad.


1966 ◽  
Vol 51 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Lillian Lindegren ◽  
Paul Starr

ABSTRACT Umbilical cord and serial venous PBI, cholesterol, TBG, and TBPA measurements were made on 300 human infants; x-ray for bone age, height age and weight age, clinical and neurological evaluations were done at birth; at 3 months 128 mothers and babies returned for these tests; and at 6 months only 52 returned. The results show an extreme range of PBI values in both mothers and babies; no correlation between the PBI of the baby and the mothers; no correlation between the umbilical cord PBI and TBG or TBPA, or the sum of these. At 3 months the babies' mean PBI and range of PBI was of the same magnitude as in the cord blood but there was no correlation between the individual values at birth and 3 months. The mean serum cholesterol in the cord blood was 90.5 mg/100 ml and 181.0 mg/100 ml at 3 months. In two thirds of the 49 infants in whom PBI values were recorded at birth and at 3 and 6 months; the variation between the lowest and highest value was frequently two or three fold. The distal femoral epiphyses were smaller in infants whose umbilical vein serum PBI concentration was less than 5 μg/100 ml as compared to those with higher PBI's. But, at three months of age, the serum PBI relationships were frequently reversed and the bone age appeared normal. Finally, at six months of age there was no consistent quantitative relationship between the serum PBI value, height age, and bone age. Fourteen children were suspected of hypothyroidism; two of 52 at six months of age were finally put on maintenance treatment with thyroxine.


1981 ◽  
Vol 33 (1) ◽  
pp. 15-17 ◽  
Author(s):  
Jean M. Bass ◽  
G. Fishwick ◽  
J. J. Parkins

ABSTRACTSixteen Hereford-cross cattle (mean live weight 263 kg) in groups of four were individually given 1·5 kg cubed barley per day with ad libitum access to oat straw. In a 4 × 4 Latin square design (21-day feeding periods) they were additionally given either no supplement (diet A) or 100g/day of a fully soluble liquid supplement containing urea (equivalent to 1090 g crude protein per kg), calcium, phosphorus, sodium, trace elements and vitamins, presented either in the cubed barley (diet B), in the drinking water (diet C) or in a molasses lick (3 parts molasses:2 parts liquid supplement:1 part water) (diet D). Each method of supplementation significantly increased the mean voluntary straw dry-matter intake from approximately 2·9 to approximately 3·25 kg. Mean blood urea concentrations were significantly increased from approximately 1·0 to approximately 1·9 mmol/1 by all three treatments. The coefficients of variation for the individual blood-urea values of the cattle when given each of the three treatments were very comparable, indicating that there was a reasonable individual uniformity of intake of liquid supplement irrespective of method of presentation.


1994 ◽  
Vol 74 (3) ◽  
pp. 503-509 ◽  
Author(s):  
L. J. Fisher ◽  
N. Dinn ◽  
R. M. Tait ◽  
J. A. Shelford

This study was undertaken with the objective of providing more basic information on the absorption and excretion of calcium (Ca) and magnesium (Mg) by lactating cows when high levels of potassium (K) are fed. Employing potassium carbonate as the source of supplemental K, total mixed rations were formulated to contain 1.6 (low), 3.1 (medium) and 4.6% (high) K. The three rations were fed to 15 Holstein cows in mid-lactation in a 3 × 3 Latin square experiment replicated five times. The experimental periods were 21 d in length. Blood, rumen and milk samples were obtained during the 2nd and 3rd wk of each experimental period and two cows from each treatment group were subjected to total collection procedures for feces and urine during the last 4 d of each experimental period. Dry matter intake was 23.0, 23.8 and 22.3 kg d−1 for the cows fed the low, medium and high K diets, respectively. Milk yield was lower (P < 0.05) for cows fed the high compared to the low and medium K diets. Level of K in the diet did not influence the fat and protein content of the milk but Ca content was lower (P < 0.05) for cows fed the medium and high K diets compared with those fed the low K diet. Plasma K levels were 15.0, 15.7 and 16.2 mg dL−1 while plasma Mg levels were 2.35a, 2.25ab and 2.17b mg dL−1 for cows on the low, medium and high K diets, respectively. The acetate to propionate ratio in the rumen fluid was wider (P < 0.05) for the cows fed the medium and high K diets compared with those fed the low K diet. The apparent absorption of Mg but not Ca was reduced with increasing levels of K in the diet. Both water consumption and urine output were greater (P < 0.05) for the cows fed the medium and high K diet compared with those fed the low K diet. The concentration of Ca and Mg in the urine dropped markedly and total excretion of Ca and Mg was lower (P < 0.05) for cows fed the medium and high K diets compared with those fed the low K diet. It was concluded from the results of this study that high K levels in the diet interfere with absorption of Mg but not Ca. However, K did appear to interfere with the utilization of Ca as indicated by the lower amount of Ca in the milk and urine. Increasing K levels in the diet increased water consumption and urine output which has major implications for waste management. Key words: Lactating cows, excess potassium, calcium, magnesium


1979 ◽  
Vol 90 (3) ◽  
pp. 481-489 ◽  
Author(s):  
M. Gourmelen ◽  
M. T. Pham-Huu-Trung ◽  
M. G. Bredon ◽  
F. Girard

ABSTRACT The variations in plasma cortisol, testosterone and 17-hydroxyprogesterone (17-OHP) induced by an im injection of 0.25 mg cosyntrophin were studied in three groups of subjects: 16 healthy women, 16 hirsute women (HW) and 10 mild cases of congenital adrenal hyperplasia (CAH). The basal values of cortisol and testosterone were comparable between the three groups. In the patients with mild CAH, the mean 17-OHP concentration was increased: 483.9 ng/100 ml (113-1200 ng), but it should be noted that the individual values could overlap with the normal concentrations found in the controls and the HW during the luteal phase of the cycle. One hour after the injection of cosyntropin, a massive response of 17-OHP was observed in the mild cases of CAH, the mean basal concentration was multiplied by ten: 4843 ng/100 ml. The minimum concentration reached was 1740 ng/100 ml which is still 3-fold the highest level seen either in normal women (400 ng/ml) or in hirsute women (550 ng/100 ml). Determination of 17-OHP following a short-term ACTH stimulation, therefore provides evidence of partial 21-hydroxylase deficiency.


1970 ◽  
Vol 64 (1) ◽  
pp. 126-132 ◽  
Author(s):  
K. Siersbæk-Nielsen ◽  
J. Mølholm Hansen

ABSTRACT Tyrosine and free thyroxine in the CSF and in serum has been measured in 24 euthyroid patients, in 11 patients with thyrotoxicosis and in 4 patients suffering from myxoedema. The thyrotoxic patients had elevated tyrosine levels in the CSF and in the serum, but rise in tyrosine was greater in the CSF than in the serum and the mean ratio CSF tyrosine/serum tyrosine was significantly elevated as compared to the euthyroid group. In the hypothyroid group, in spite of decreased serum tyrosine values the CSF tyrosine was not decreased. Free thyroxine in the CSF was elevated in the thyrotoxic patients and decreased in the hypothyroid patients. In euthyroid, hyperthyroid and hypothyroid patients an equilibrium was found between free thyroxine in the CSF and in the serum. No correlation was found within the groups between the individual values of tyrosine in the serum and in the CSF or between tyrosine and free thyroxine in the serum and in the CSF.


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