Chronic stress induces ageing-associated degeneration in rat Leydig cells

The experiment on male Wistar rats has demonstrated that the exposure to modelled chronic stress for 10 days causes disturbances of spermatogenesis processes and exerts influence on the decrease of the relative number of Leydig cells in the rats` testis. It has been found that after 10 days of the chronic stress modelling by Ortiz J., the number of active forms of the Leydig cells decreases and the number of non-active forms of steroid-producing cells increases in the interstitial tissue of the testis of the Wistar rats.

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Effects of nutrition, social factors and chronic stress on the mouse Leydig cell testosterone production

Veterinární Medicína ◽

10.17221/7874-vetmed ◽

2001 ◽

Vol 46 (No. 6) ◽

pp. 160-168

Author(s):

L. Faldíková ◽

I. Diblíková ◽

J. Čanderle ◽

Z. Zralý ◽

Z. Věžník ◽

...

Keyword(s):

Chronic Stress ◽

Social Factors ◽

Leydig Cells ◽

Serum Testosterone ◽

Potassium Nitrate ◽

Male Mice ◽

Nutritional Factors ◽

Testosterone Production ◽

The Impact

The testosterone production by Leydig cells of mice adversely affected with nutritional or social factors and exposed to chronic stress was studied in vitro. Both basal and gonadotrophin stimulated testosterone production were highly significantly decreased (P < 0.01) as in groups given the hypothyreotics or potassium nitrate alone as in combination with stress. Stimulated in vitro testosterone production was not significantly changed in groups fed with synthetic diets, however, in mice given modified diets and exposed to stress decreased responsivity of Leydig cells stimulated by gonadotrophin was found. Basal and stimulated testosterone production in vitro in most of gonadotrophin concentrations was non-significantly lower in mice affected only by stress when compared with undisturbed controls. In isolated male mice the basal testosterone production was significantly (P < 0.05) decreased and gonadotrophin stimulated production highly significantly (P < 0.01) decreased when compared with group caged males. The testosterone production was most severely suppressed in aggressive individuals. Serum testosterone levels were detected in all animals, corticosterone, T3and T4 in selected groups of mice. We can conclude that the testosterone production was adversely affected by nutritional factors, and the impact was more profound when exerted together with chronic stress. The adverse effect of individual caging of male mice was also proved.

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Review for "Anti‐Lingo ‐1 antibody ameliorates spatial memory and synapse loss induced by chronic stress"

10.1002/cne.25038/v1/review2 ◽

2020 ◽

Keyword(s):

Spatial Memory ◽

Chronic Stress ◽

Synapse Loss

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Ultrastructural Study of Human Testicular Biopsies in Infertility

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090762 ◽

1976 ◽

Vol 34 ◽

pp. 156-157

Author(s):

Shirley Siew ◽

Philip Troen ◽

Howard R. Nankin

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Basement Membrane ◽

Leydig Cells ◽

Clinical Evidence ◽

Young Male ◽

Germinal Epithelium ◽

Transmission Electron ◽

Age Range ◽

Male Subjects

Testicular biopsies were obtained from six young male subjects (age range 24-33) who complained of infertility and who had clinical evidence of oligospermia. This was confirmed on histological examination which showed a broad spectrum from profound hypospermatogenesis to relatively normal appearing germinal epithelium. Thickening of the tubular walls was noted in half of the cases and slight peritubular fibrosis in one. The Leydig cells were reported as normal or unremarkable.Transmission electron microscopy showed that the thickening of the supporting tissue of the germinal epithelium was caused more by an increase in the thickness of the layers of the lamina propria than of the tubular wall itself. The changes in the basement membrane of the tubular wall consisted mostly of a greater degree of infolding into the tubule and some reduplication which gave rise to a multilayered appearance.

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The structure of the gills of the axolotl, Ambystoma mexicanum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128407 ◽

1987 ◽

Vol 45 ◽

pp. 824-825

Author(s):

Mohinder S. Jarial

Keyword(s):

Leydig Cells ◽

Secretory Granules ◽

Light And Electron Microscopy ◽

Cell Types ◽

Ambystoma Mexicanum ◽

Basal Cells ◽

Granular Cells ◽

Gill Filaments ◽

Mitotic Figures ◽

Apical Membranes

The axolotl is a strictly aquatic salamander in which the larval external gills are retained throughout life. The external gills of the adult axolotl have been studied by light and electron microscopy for ultrastructural evidence of ionic transport. The thin epidermis of the gill filaments and gill stems is composed of 3 cell types: granular cells, the basal cells and a sparce population of intervening Leydig cells. The gill epidermis is devoid of muscles, and no mitotic figures were observed in any of its cells.The granular cells cover the gill surface as a continuous layer (Fig. 1, G) and contain secretory granules of different forms, located apically (Figs.1, 2, SG). Some granules are found intimately associated with the apical membrane while others fuse with it and release their contents onto the external surface (Fig. 3). The apical membranes of the granular cells exhibit microvilli which are covered by a PAS+ fuzzy coat, termed “glycocalyx” (Fig. 2, MV).

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Smooth endoplasmic reticulum in perfusion and immersion-fixed Leydig cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157954 ◽

1990 ◽

Vol 48 (3) ◽

pp. 82-83

Author(s):

R.T.F. Bernard ◽

R.H.M. Cross

Keyword(s):

Endoplasmic Reticulum ◽

Electron Microscope ◽

Steroid Hormones ◽

Transmission Electron Microscope ◽

Leydig Cells ◽

Smooth Endoplasmic Reticulum ◽

Random Mixture ◽

Transmission Electron

Smooth endoplasmic reticulum (SER) is involved in the biosynthesis of steroid hormones, and changes in the organisation and abundance of this organelle are regularly used as indicators of changes in the level of steroidogenesis. SER is typically arranged as a meshwork of anastomosing tubules which, with the transmission electron microscope, appear as a random mixture of cross, oblique and longitudinal sections. Less commonly the SER appears as swollen vesicles and it is generally suggested that this is an artefact caused during immersion fixation or during immersion of poorly-perfused tissue.During a previous study of the Leydig cells of a seasonally reproducing bat, in which tissue was fixed by immersion, we noted that tubular SER and vesicular SER often occured in adjacent cells and sometimes in the same cell, and that the abundance of the two types of SER changed seasonally. We came to doubt the widelyheld dogma that vesicular SER was an artefact of immersion fixation and set out to test the hypothesis that the method of fixation does not modify the ultrastructure of the SER.

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