scholarly journals The Effects of Delayed Reduction of Tonic Inhibition on Ischemic Lesion and Sensorimotor Function

2015 ◽  
Vol 35 (10) ◽  
pp. 1601-1609 ◽  
Author(s):  
Evelyn MR Lake ◽  
Joydeep Chaudhuri ◽  
Lynsie Thomason ◽  
Rafal Janik ◽  
Milan Ganguly ◽  
...  
Keyword(s):  
Rat Model ◽  
Focal Ischemia ◽  
Stroke Recovery ◽  
Gamma Aminobutyric Acid ◽  
Ischemic Lesion ◽  
Chronic Stage ◽  
Beneficial Effects ◽  
Sensorimotor Deficits ◽  
Skilled Reaching ◽  
Better Than

To aid in development of chronic stage treatments for sensorimotor deficits induced by ischemic stroke, we investigated the effects of GABA antagonism on brain structure and fine skilled reaching in a rat model of focal ischemia induced via cortical microinjections of endothelin-1 (ET-1). Beginning 7 days after stroke, animals were administered a gamma-aminobutyric acid (GABAA) inverse agonist, L-655,708, at a dose low enough to afford α5-GABAA receptor specificity. A week after stroke, the ischemic lesion comprised a small hypointense necrotic core (6 ± 1 mm3) surrounded by a large (62 ± 11 mm3) hyperintense perilesional region; the skilled reaching ability on the Montoya staircase test was decreased to 34% ± 2% of the animals' prestroke performance level. On L-655,708 treatment, animals showed a progressive decrease in total stroke volume (13 ± 4 mm3 per week), with no change in animals receiving placebo. Concomitantly, treated animals' skilled reaching progressively improved by 9% ± 1% per week, so that after 2 weeks of treatment, these animals performed at 65% ± 6% of their baseline ability, which was 25% ± 11% better than animals given placebo. These data indicate beneficial effects of delayed, sustained low-dose GABAA antagonism on neuroanatomic injury and skilled reaching in the chronic stage of stroke recovery in an ET-1 rat model of focal ischemia.

Use of Diffusion-Weighted MRI and Neurological Deficit Scores to Demonstrate Beneficial Effects of Isradipine in a Rat Model of Focal Ischemia

Pharmacology ◽  
1999 ◽  
Vol 58 (6) ◽  
pp. 292-299 ◽  
Author(s):  
Sudeep Chandra ◽  
Ray F. White ◽  
Daryl Everding ◽  
Giora Z. Feuerstein ◽  
Robert W. Coatney ◽  
...  
Keyword(s):  
Rat Model ◽  
Neurological Deficit ◽  
Focal Ischemia ◽  
Diffusion Weighted Mri ◽  
Beneficial Effects ◽  
Diffusion Weighted

scholarly journals Beneficial Effects of a Fermented Maize product with Its Supernatant, Lactobacillus fermentum and Lactobacillus brevis in Rat Model of Colitis

2019 ◽  
Vol 3 (6) ◽  
pp. 195-200
Author(s):  
Haruna J. Audu ◽  
Oyindamola A. Abiodun ◽  
Funmilola A. Ayeni
Keyword(s):  
Rat Model ◽  
Wistar Rats ◽  
Lactobacillus Brevis ◽  
Fermented Food ◽  
Fermented Foods ◽  
Myeloperoxidase Activity ◽  
Treatment Groups ◽  
Beneficial Effects ◽  
Female Wistar Rats ◽  
Better Than

Background: African fermented foods such as maize cereal (Ogi ) and its supernatant (Omidun) are reservoirs of beneficial lactobacilli and carboxylic acids. Based on their constituents, their beneficial effect in a rat model of 2,4,6-trinitrobenzene sulphonic (TNBS) acid-induced colitis was assessed in comparison with two lactobacilli in a rat model of colitis. Methods: Female Wistar rats were distributed into seven groups of 5 rats each; the rats were pretreated for 14 days prior to colitis induction with Ogi, Omidun, L. fermentum, L. brevis and 7 days post colitic induction. Colitis was induced by an intracolonic administration of TNBS. The response of the rats to treatment was assessed macroscopically and biochemically. Results: Treatment with the Dexamethasone, Ogi and Omidun resulted in a significant reduction in colonic damage score and weight/length ratio (p<0.05). Treatment with Ogi, Omidun, L. brevis, and dexamethasone significantly prevented depletion of colonic glutathione and superoxide dismutase. The up-regulation of myeloperoxidase activity was inhibited in all treated colitic rats (p<0.05). However, Ogi appears to produce a better protective effect than the other treatment groups. Conclusions: This study reports that Ogi protects Wistar rats against the deleterious effect of trinitrobenzene sulphonic acid better than pure lactobacilli strains. Keywords: Fermented food, lactic acid bacteria, inflammation, oxidative stress.

Uric acid improves the beneficial effects of rt-PA in a rat model of thromboembolic ischemia

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S129-S129
Author(s):  
Eduardo Romanos ◽  
Anna M Planas ◽  
Angel Chamorro
Keyword(s):  
Uric Acid ◽  
Rat Model ◽  
Beneficial Effects

scholarly journals A rat model of organophosphate-induced status epilepticus and the beneficial effects of EP2 receptor inhibition

2020 ◽  
Vol 133 ◽  
pp. 104399 ◽  
Author(s):  
Asheebo Rojas ◽  
Thota Ganesh ◽  
Wenyi Wang ◽  
Jennifer Wang ◽  
Raymond Dingledine
Keyword(s):  
Status Epilepticus ◽  
Rat Model ◽  
Beneficial Effects ◽  
Receptor Inhibition ◽  
Ep2 Receptor

scholarly journals In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination

Diagnostics ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 45
Author(s):  
Do-Wan Lee ◽  
Jae-Im Kwon ◽  
Chul-Woong Woo ◽  
Hwon Heo ◽  
Kyung Won Kim ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Rat Model ◽  
Resonance Spectroscopy ◽  
Chow Diet ◽  
Gamma Aminobutyric Acid ◽  
Sprague Dawley ◽  
In Vivo Measurement ◽  
Hippocampal Lesions ◽  
Total Creatine

This study quantitatively measured the changes in metabolites in the hippocampal lesions of a rat model of cuprizone-induced demyelination as detected using in vivo 7 T proton magnetic resonance spectroscopy. Nineteen Sprague Dawley rats were randomly divided into two groups and fed a normal chow diet or cuprizone (0.2%, w/w) for 7 weeks. Demyelinated hippocampal lesions were quantitatively measured using a 7 T magnetic resonance imaging scanner. All proton spectra were quantified for metabolite concentrations and relative ratios. Compared to those in the controls, the cuprizone-induced rats had significantly higher concentrations of glutamate (p = 0.001), gamma-aminobutyric acid (p = 0.019), and glutamate + glutamine (p = 0.001); however, creatine + phosphocreatine (p = 0.006) and myo-inositol (p = 0.001) concentrations were lower. In addition, we found that the glutamine and glutamate complex/total creatine (p < 0.001), glutamate/total creatine (p < 0.001), and GABA/total creatine (p = 0.002) ratios were significantly higher in cuprizone-treated rats than in control rats. Our results showed that cuprizone-induced neuronal demyelination may influence the severe abnormal metabolism in hippocampal lesions, and these responses could be caused by microglial activation, mitochondrial dysfunction, and astrocytic necrosis.

scholarly journals A Collagen-Based Scaffold for Promoting Neural Plasticity in a Rat Model of Spinal Cord Injury

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2245
Author(s):  
Jue-Zong Yeh ◽  
Ding-Han Wang ◽  
Juin-Hong Cherng ◽  
Yi-Wen Wang ◽  
Gang-Yi Fan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Rat Model ◽  
Neural Plasticity ◽  
Collagen Scaffold ◽  
Glial Scar ◽  
Beneficial Effects ◽  
Cord Injury

In spinal cord injury (SCI) therapy, glial scarring formed by activated astrocytes is a primary problem that needs to be solved to enhance axonal regeneration. In this study, we developed and used a collagen scaffold for glial scar replacement to create an appropriate environment in an SCI rat model and determined whether neural plasticity can be manipulated using this approach. We used four experimental groups, as follows: SCI-collagen scaffold, SCI control, normal spinal cord-collagen scaffold, and normal control. The collagen scaffold showed excellent in vitro and in vivo biocompatibility. Immunofluorescence staining revealed increased expression of neurofilament and fibronectin and reduced expression of glial fibrillary acidic protein and anti-chondroitin sulfate in the collagen scaffold-treated SCI rats at 1 and 4 weeks post-implantation compared with that in untreated SCI control. This indicates that the collagen scaffold implantation promoted neuronal survival and axonal growth within the injured site and prevented glial scar formation by controlling astrocyte production for their normal functioning. Our study highlights the feasibility of using the collagen scaffold in SCI repair. The collagen scaffold was found to exert beneficial effects on neuronal activity and may help in manipulating synaptic plasticity, implying its great potential for clinical application in SCI.

BENEFICIAL EFFECTS OF GPI 6150, AN INHIBITOR OF POLY(ADP-RIBOSE) POLYMERASE IN A RAT MODEL OF SPLANCHNIC ARTERY OCCLUSION AND REPERFUSION.

Shock ◽  
2001 ◽  
Vol 15 (Supplement) ◽  
pp. 58-59
Author(s):  
E. Mazzon ◽  
L. Dugo ◽  
A. De Sarro ◽  
J. Li ◽  
A. P. Caputi ◽  
...  
Keyword(s):  
Rat Model ◽  
Artery Occlusion ◽  
Beneficial Effects ◽  
Splanchnic Artery

scholarly journals Local application of vancomycin in one-stage revision of PJI caused by MRSA in a rat model

2021 ◽  
Author(s):  
Jian Wei ◽  
Yinxian Wen ◽  
Kai Tong ◽  
Hui Wang ◽  
Liaobin Chen
Keyword(s):  
Rat Model ◽  
Revision Surgery ◽  
Eradication Rate ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Joint Infection ◽  
Revision Arthroplasty ◽  
Serum Markers ◽  
Acid Glycoprotein ◽  
One Stage ◽  
Better Than

The eradication rate of periprosthetic joint infection (PJI) caused by methicillin-resistant staphylococcus aureus (MRSA) is still not satisfactory by systemic vancomycin administration in one-stage revision arthroplasty. This study aimed to explore the effectiveness and safety of intra-articular injection of vancomycin in the control of MRSA-PJI after one-stage revision surgery in a rat model. Two weeks of intra-peritoneal (IP) and/or intra-articular (IA) injection of vancomycin were applied to control the infection after one-stage revision surgery. The MRSA-PJI rats treated with IA injection of vancomycin showed a better outcome in skin temperature, bacterial counts, biofilm on the prosthesis, serum alpha-1-acid glycoprotein (α1-AGP), residual bone volume and inflammatory reaction in the joint tissues than those with IP vancomycin, while rats with IP & IA administration showed the best outcomes. However, only the IP & IA administration of vancomycin could eradicate MRSA. Minimal changes of renal pathology were observed in IP and IP & IA groups, rather than IA group, while no obvious changes were observed in the liver, as well as serum markers including creatinine (Cr), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Therefore, IA use of vancomycin is effective and safe in the MRSA-PJI rat model, better than systematic administration, while IA & systemic vancomycin could eradicate the infection in a two-week treatment course.

Discovery of Serum Proteomic Biomarkers for Prediction of Response to Moxibustion Treatment in Rats with Collagen-Induced Arthritis: An Exploratory Analysis

2016 ◽  
Vol 34 (3) ◽  
pp. 184-193 ◽  
Author(s):  
Xiao Xu ◽  
Miao-Miao Wang ◽  
Zhi-ling Sun ◽  
Dan-ping Zhou ◽  
Ling Wang ◽  
...  
Keyword(s):  
Rat Model ◽  
Multiple Testing ◽  
Day Treatment ◽  
Expression Patterns ◽  
Control Group ◽  
Sprague Dawley ◽  
Collagen Induced Arthritis ◽  
Serum Samples ◽  
Beneficial Effects ◽  
Bovine Collagen

Objective To examine the possible impact of moxibustion on the serum proteome of the collagen-induced arthritis (CIA) rat model. Materials and Methods Thirty-six male Sprague-Dawley rats were included in this experiment. The CIA animal model was prepared by injection of type II bovine collagen in Freund's adjuvant on the first and seventh day. The 36 rats were randomly divided into two groups: the untreated CIA group (control), and the CIA plus treatment with moxibustion (CIA+moxi) group. Moxibustion was administered daily at ST36 and BL23 for 7, 14 or 21 days (n=12 rats each). Arthritis score was used to assess the severity of arthritis. At the end of each 7 day treatment, blood samples from the control group and the CIA+moxi group were collected. After removal of high abundance proteins from serum samples, two-dimensional gel combined with matrix-assisted laser desorption ionisation time-of-flight MS/MS (MALDI-TOF-MS/MS) techniques were performed to examine serum protein expression patterns of the CIA rat model with and without moxibustion treatment. In addition, the relevant proteins were further analysed with the use of bioinformatics analysis. Results Moxibustion significantly decreased arthritis severity in the rats in the CIA+moxi group, when compared with the rats in the CIA group 35 days after the first immunisation (p=0.001). Seventeen protein spots which changed >1.33 or <0.77 at p<0.05 using Bonferonni correction for multiple testing were found to be common to all three comparisons, and these proteins were used for classification of functions using the Gene Ontology method. Consequently, with the use of the Ingenuity Pathway Analysis, the top canonical pathways and a predicted proteomic network related to the moxibustion effect of CIA were established. Conclusions Using the proteomics technique, we have identified novel candidate proteins that may be involved in the mechanisms of action underlying the beneficial effects of moxibustion in rats with CIA. Our findings suggest that immune responses and metabolic processes may be involved in mediating the effects of moxibustion. Moreover, periodxiredoxin I (PRDX1) and inositol 1,4,5-triphosphate receptor (IP3R) may be potential targets.

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