scholarly journals Phosphorus deficiencies invoke optimal allocation of exoenzymes by ectomycorrhizas

2021 ◽  
Author(s):  
Justin A. Meeds ◽  
J. Marty Kranabetter ◽  
Ieva Zigg ◽  
Dave Dunn ◽  
François Miros ◽  
...  
Keyword(s):  
Optimal Allocation ◽  
Resource Constraints ◽  
Species Turnover ◽  
Hydrolytic Enzymes ◽  
Fungal Species ◽  
Fungal Communities ◽  
P Availability ◽  
Soil P ◽  
Organic Horizons ◽  
Forest Floors

AbstractEctomycorrhizal (EM) fungi can acquire phosphorus (P) through the production of extracellular hydrolytic enzymes (exoenzymes), but it is unclear as to the manner and extent native EM fungal communities respond to declining soil P availability. We examined the activity of six exoenzymes (xylosidase, N-acetyl glucosaminidase, β-glucosidase, acid phosphomonoesterase, acid phosphodiesterase [APD], laccase) from EM roots of Pseudotsuga menzesii across a soil podzolization gradient of coastal British Columbia. We found that APD activity increased fourfold in a curvilinear association with declining inorganic P. Exoenzyme activity was not related to organic P content, but at a finer resolution using 31P-NMR, there was a strong positive relationship between APD activity and the ratio of phosphodiesters to orthophosphate of surface organic horizons (forest floors). Substantial increases (two- to fivefold) in most exoenzymes were aligned with declining foliar P concentrations of P. menzesii, but responses were statistically better in relation to foliar nitrogen (N):P ratios. EM fungal species with consistently high production of key exoenzymes were exclusive to Podzol plots. Phosphorus deficiencies in relation to N limitations may provide the best predictor of exoenzyme investment, reflecting an optimal allocation strategy for EM fungi. Resource constraints contribute to species turnover and the assembly of distinct, well-adapted EM fungal communities.

Improved Plant Diversity as a Strategy to Increase Available Soil Phosphorus

2019 ◽  
Vol 103 (1) ◽  
pp. 43-45 ◽  
Author(s):  
Carlos Crusciol ◽  
João Rigon ◽  
Juliano Calonego ◽  
Rogério Soratto
Keyword(s):  
Crop Yields ◽  
Soil Phosphorus ◽  
Cropping System ◽  
P Availability ◽  
P Fractions ◽  
Crop Species ◽  
Soil P ◽  
Residue Decomposition ◽  
Available Soil Phosphorus ◽  
Crop Residue Decomposition

Some crop species could be used inside a cropping system as part of a strategy to increase soil P availability due to their capacity to recycle P and shift the equilibrium between soil P fractions to benefit the main crop. The release of P by crop residue decomposition, and mobilization and uptake of otherwise recalcitrant P are important mechanisms capable of increasing P availability and crop yields.

Antifungal Proteins from Plant Latex

2020 ◽  
Vol 21 (5) ◽  
pp. 497-506
Author(s):  
Mayck Silva Barbosa ◽  
Bruna da Silva Souza ◽  
Ana Clara Silva Sales ◽  
Jhoana D’arc Lopes de Sousa ◽  
Francisca Dayane Soares da Silva ◽  
...  
Keyword(s):  
Cell Walls ◽  
Defense Mechanisms ◽  
Hydrolytic Enzymes ◽  
Fungal Species ◽  
Biologically Active ◽  
Protein Classification ◽  
Fungal Cell ◽  
Antifungal Proteins ◽  
Plant Latex

Latex, a milky fluid found in several plants, is widely used for many purposes, and its proteins have been investigated by researchers. Many studies have shown that latex produced by some plant species is a natural source of biologically active compounds, and many of the hydrolytic enzymes are related to health benefits. Research on the characterization and industrial and pharmaceutical utility of latex has progressed in recent years. Latex proteins are associated with plants’ defense mechanisms, against attacks by fungi. In this respect, there are several biotechnological applications of antifungal proteins. Some findings reveal that antifungal proteins inhibit fungi by interrupting the synthesis of fungal cell walls or rupturing the membrane. Moreover, both phytopathogenic and clinical fungal strains are susceptible to latex proteins. The present review describes some important features of proteins isolated from plant latex which presented in vitro antifungal activities: protein classification, function, molecular weight, isoelectric point, as well as the fungal species that are inhibited by them. We also discuss their mechanisms of action.

Decreases in soil P availability are associated with soil organic P declines following forest conversion in subtropical China

CATENA ◽  
2021 ◽  
Vol 205 ◽  
pp. 105459
Author(s):  
Liuming Yang ◽  
Zhijie Yang ◽  
Xiaojian Zhong ◽  
Chao Xu ◽  
Yanyu Lin ◽  
...  
Keyword(s):  
Forest Conversion ◽  
P Availability ◽  
Organic P ◽  
Subtropical China ◽  
Soil P ◽  
Soil P Availability

The use of biologically based phosphorus fractions to evaluate soil P availability in reduced P-input paddy soils

2018 ◽  
Vol 34 (3) ◽  
pp. 326-334 ◽  
Author(s):  
J. Yuan ◽  
L. Wang ◽  
S. Wang ◽  
Y. Wang ◽  
H. Wang ◽  
...  
Keyword(s):  
Phosphorus Fractions ◽  
Paddy Soils ◽  
P Availability ◽  
Soil P ◽  
Soil P Availability

scholarly journals Modifications of phosphorus in Latosol as a function of humic acids and acidity

2018 ◽  
Vol 22 (7) ◽  
pp. 488-492 ◽  
Author(s):  
Márcia H. Beck ◽  
Pedro A. V. Escosteguy ◽  
Deborah P. Dick
Keyword(s):  
Factorial Design ◽  
Humic Acids ◽  
Soil Ph ◽  
Crucial Role ◽  
Soil Acidity ◽  
Ph Value ◽  
P Availability ◽  
Soil P ◽  
P Content

ABSTRACT The effect of humic acids (HA) on phosphorus (P) availability is still contradictory; thus, it is necessary to identify the conditions that play a crucial role in this effect. The aim of this study was to investigate the effect of HA application, combined with doses of P, on the content of this nutrient in a Latosol with and without acidity correction. Two experiments were carried out, one with HA from peat and another with HA from mineral charcoal (leonardite). Doses of these acids (0; 1.12 and 5.62 mg C g-1 of soil) and P (26.2 and 104.7 mg P g-1 of soil, 1 and 4-fold higher than recommended, respectively) were tested at soil pH 4.5 and 7.0, in a three-factorial design. The soil was incubated for 20 days and the soil-P content was measured by Mehlich-1 and remaining-P tests. The effect of HAs on P availability varied with the P doses and soil acidity. Humic acids application increases P content in Latosol when P dose is higher than recommended and there is no acidity correction (pH 4.5). However, there is no effect of HAs application on soil-P content when applying the recommended amount of this nutrient, irrespective of the pH value.

scholarly journals Different Amplicon Targets for Sequencing-Based Studies of Fungal Diversity

2017 ◽  
Vol 83 (17) ◽  
Author(s):  
Francesca De Filippis ◽  
Manolo Laiola ◽  
Giuseppe Blaiotta ◽  
Danilo Ercolini
Keyword(s):  
Microbial Ecology ◽  
High Throughput ◽  
Biological Samples ◽  
Fungal Diversity ◽  
High Throughput Sequencing ◽  
Amplicon Sequencing ◽  
Fungal Species ◽  
Fungal Communities ◽  
Its2 Region ◽  
Mock Community

ABSTRACT Target-gene amplicon sequencing is the most exploited high-throughput sequencing application in microbial ecology. The targets are taxonomically relevant genes, with 16S rRNA being the gold standard for bacteria. As for fungi, the most commonly used target is the internal transcribed spacer (ITS). However, the uneven ITS length among species may promote preferential amplification and sequencing and incorrect estimation of their abundance. Therefore, the use of different targets is desirable. We evaluated the use of three different target amplicons for the characterization of fungal diversity. After an in silico primer evaluation, we compared three amplicons (the ITS1-ITS2 region [ITS1-2], 18S ribosomal small subunit RNA, and the D1/D2 domain of the 26S ribosomal large subunit RNA), using biological samples and a mock community of common fungal species. All three targets allowed for accurate identification of the species present. Nevertheless, high heterogeneity in ITS1-2 length was found, and this caused an overestimation of the abundance of species with a shorter ITS, while both 18S and 26S amplicons allowed for more reliable quantification. We demonstrated that ITS1-2 amplicon sequencing, although widely used, may lead to an incorrect evaluation of fungal communities, and efforts should be made to promote the use of different targets in sequencing-based microbial ecology studies. IMPORTANCE Amplicon-sequencing approaches for fungi may rely on different targets affecting the diversity and abundance of the fungal species. An increasing number of studies will address fungal diversity by high-throughput amplicon sequencing. The description of the communities must be accurate and reliable in order to draw useful insights and to address both ecological and biological questions. By analyzing a mock community and several biological samples, we demonstrate that using different amplicon targets may change the results of fungal microbiota analysis, and we highlight how a careful choice of the target is fundamental for a thorough description of the fungal communities.

scholarly journals Diversity, host-specificity and stability of sponge-associated fungal communities of co-occurring sponges

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4965 ◽  
Author(s):  
Mary T.H.D. Nguyen ◽  
Torsten Thomas
Keyword(s):  
Host Specificity ◽  
Horizontal Transmission ◽  
Critical Role ◽  
Fungal Species ◽  
Fungal Communities ◽  
Sponge Species ◽  
Fungal Community Composition ◽  
Potential Core ◽  
Community Profiling ◽  
Surrounding Seawater

Fungi play a critical role in a range of ecosystems; however, their interactions and functions in marine hosts, and particular sponges, is poorly understood. Here we assess the fungal community composition of three co-occurring sponges (Cymbastela concentrica, Scopalina sp., Tedania anhelans) and the surrounding seawater over two time points to help elucidate host-specificity, stability and potential core members, which may shed light into the ecological function of fungi in sponges. The results showed that ITS-amplicon-based community profiling likely provides a more realistic assessment of fungal diversity in sponges than cultivation-dependent approaches. The sponges studied here were found to contain phylogenetically diverse fungi (eight fungal classes were observed), including members of the family Togniniaceae and the genus Acrostalagmus, that have so far not been reported to be cultured from sponges. Fungal communities within any given sponge species were found to be highly variable compared to bacterial communities, and influenced in structure by the community of the surrounding seawater, especially considering temporal variation. Nevertheless, the sponge species studied here contained a few “variable/core” fungi that appeared in multiple biological replicates and were enriched in their relative abundance compared to seawater communities. These fungi were the same or highly similar to fungal species detected in sponges around the world, which suggests a prevalence of horizontal transmission where selectivity and enrichment of some fungi occur for those that can survive and/or exploit the sponge environment. Our current sparse knowledge about sponge-associated fungi thus indicate that fungal communities may perhaps not play as an important ecological role in the sponge holobiont compared to bacterial or archaeal symbionts.

scholarly journals Ericoid mycorrhizal diversity increases with soil age and progressive phosphorus limitation across a 4.1 million-year chronosequence

2020 ◽  
Author(s):  
Devin R. Leopold ◽  
Kabir G. Peay ◽  
Peter M. Vitousek ◽  
Tadashi Fukami
Keyword(s):  
Species Composition ◽  
Nutrient Limitation ◽  
Mycorrhizal Fungi ◽  
Fungal Diversity ◽  
Species Turnover ◽  
Phosphorus Limitation ◽  
Fungal Species ◽  
Direct Role ◽  
Ericoid Mycorrhizal Fungi ◽  
Soil Age

AbstractEricaceous plants rely on ericoid mycorrhizal fungi for nutrient acquisition. However, the factors that affect the composition and structure of these fungal communities remain largely unknown. Here, we use a 4.1-myr soil chronosequence in Hawaii to test the hypothesis that changes in nutrient availability with soil age determine the diversity and species composition of fungi associated with ericoid roots. We sampled roots of a native Hawaiian plant, Vaccinium calycinum, and used DNA metabarcoding to quantify changes in fungal diversity and species composition. We also used a fertilization experiment at the youngest and oldest sites to assess the importance of nutrient limitation. We found an increase in diversity and a clear pattern of species turnover across the chronosequence, driven largely by putative ericoid mycorrhizal fungi. Fertilization with nitrogen at the youngest site and phosphorus at the oldest site reduced total fungal diversity, suggesting a direct role of nutrient limitation. Our results also reveal the presence of novel fungal species associated with Hawaiian Ericaceae and suggest a greater importance of phosphorus availability for communities of ericoid mycorrhizal fungi than is generally assumed.

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