scholarly journals A three-dimensional in vitro model of the peripheral nervous system

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Sunghee Estelle Park ◽  
Jinchul Ahn ◽  
Hyo-Eun Jeong ◽  
Inchan Youn ◽  
Dongeun Huh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Nervous System ◽  
Peripheral Nervous System ◽  
In Vitro Model ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Nerve Fibers ◽  
Coculture Model ◽  
Vitro Model

AbstractRecent advances in three-dimensional (3D) cell culture models developed on organ-on-a-chip or microfluidic devices have shown their capability to recapitulate the in vivo microenvironment as well as their potential as tools in biomedical research. Here, we present an in vitro model of the peripheral nervous system (PNS) by establishing a coculture model of motor neurons (MNs) and Schwann cells (SCs) in a 3D environment in a microengineered extracellular matrix hydrogel scaffold. The collagen scaffold placed at the center of the microdevice provided a 3D cellular microenvironment where the axons of MNs were allowed to actively interact with SCs during their growth and maturation. By treating the MN–SC coculture model with ascorbic acid, we were able to model the myelination process in the PNS, which was evidenced by the increased expression of myelin markers in SCs. Moreover, we show that this can be reversed by treating myelinated nerve fibers with glial growth factor (neuregulin-1 isoform) to potentially block the formation of the myelin sheath and induce demyelination. Our 3D cell culture model may be used to achieve active control of the myelinating and demyelinating processes in the PNS and thus may offer new opportunities to study pathophysiological processes involved in motor neuron diseases by in vitro modeling.

Three-dimensional microtissues as an in vitro model for personalized radiation therapy

The Analyst ◽  
2017 ◽  
Vol 142 (19) ◽  
pp. 3605-3612 ◽  
Author(s):  
Yuting Qiu ◽  
Dandan Ning ◽  
Peipei Zhang ◽  
Stephanie Curly ◽  
Yong Qiao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Dna Damage ◽  
Animal Model ◽  
Radiation Therapy ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Intermediate Model ◽  
Radiation Induced ◽  
Vitro Model

This paper describes the use of 3D microtissues as an intermediate model between the 2D cell culture and the animal model to assess radiation-induced cellular and DNA damage in the context of personalized radiation therapy.

Development of an In Vitro Model of Inflammatory Cytokine Influences on Intervertebral Disk Cells in 3D Cell Culture Using Activated Macrophage-Like THP-1 Cells

2018 ◽  
Vol 166 (1) ◽  
pp. 151-154 ◽  
Author(s):  
V. A. Byvaltsev ◽  
S. I. Kolesnikov ◽  
L. A. Bardonova ◽  
E. G Belykh ◽  
L. I. Korytov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Inflammatory Cytokine ◽  
In Vitro Model ◽  
3D Cell Culture ◽  
Intervertebral Disk ◽  
Activated Macrophage ◽  
Vitro Model

scholarly journals A 3D disease and regeneration model of peripheral nervous system–on–a–chip

2021 ◽  
Vol 7 (5) ◽  
pp. eabd9749
Author(s):  
Sujin Hyung ◽  
Seung-Ryeol Lee ◽  
Jiho Kim ◽  
Youngtaek Kim ◽  
Suryong Kim ◽  
...  
Keyword(s):  
Nervous System ◽  
Peripheral Nervous System ◽  
Motor Neurons ◽  
Three Dimensional ◽  
Demyelinating Diseases ◽  
Full Spectrum ◽  
Vitro Model ◽  
Design Factors

Demyelinating diseases involve loss of myelin sheaths and eventually lead to neurological problems. Unfortunately, the precise mechanisms remain unknown, and there are no effective therapies. To overcome these limitations, a reliable and physiologically relevant in vitro model is required. Here, we present a three-dimensional peripheral nervous system (PNS) microfluidic platform that recapitulates the full spectrum of myelination, demyelination, and remyelination using primary Schwann cells (SCs) and motor neurons (MNs). The platform enables reproducible hydrogel patterning and long-term stable coculture of MNs and SCs over 40 days in vitro based on three distinct design factors. Furthermore, the on-demand detachable substrate allows in-depth biological analysis. We demonstrated the possibility of mimicking segmental demyelination by lysophosphatidylcholine, and recovery of myelin structure by application of two drugs: benzatropine or methylcobalamin. This 3D PNS disease–on–a–chip may serve as a potential platform for understanding the pathophysiology of demyelination and screening drugs for remyelination.

scholarly journals Mammary gland 3D cell culture systems in farm animals

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Laurence Finot ◽  
Eric Chanat ◽  
Frederic Dessauge
Keyword(s):  
Cell Culture ◽  
Mammary Gland ◽  
Bacterial Infections ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Farm Animals ◽  
Culture Systems ◽  
Cell Culture Systems

AbstractIn vivo study of tissue or organ biology in mammals is very complex and progress is slowed by poor accessibility of samples and ethical concerns. Fortunately, however, advances in stem cell identification and culture have made it possible to derive in vitro 3D “tissues” called organoids, these three-dimensional structures partly or fully mimicking the in vivo functioning of organs. The mammary gland produces milk, the source of nutrition for newborn mammals. Milk is synthesized and secreted by the differentiated polarized mammary epithelial cells of the gland. Reconstructing in vitro a mammary-like structure mimicking the functional tissue represents a major challenge in mammary gland biology, especially for farm animals for which specific agronomic questions arise. This would greatly facilitate the study of mammary gland development, milk secretion processes and pathological effects of viral or bacterial infections at the cellular level, all with the objective of improving milk production at the animal level. With this aim, various 3D cell culture models have been developed such as mammospheres and, more recently, efforts to develop organoids in vitro have been considerable. Researchers are now starting to draw inspiration from other fields, such as bioengineering, to generate organoids that would be more physiologically relevant. In this chapter, we will discuss 3D cell culture systems as organoids and their relevance for agronomic research.

A rhabdomyosarcoma hydrogel model to unveil cell-extracellular matrix interactions

2021 ◽  
Author(s):  
Mattia Saggioro ◽  
Stefania D'Agostino ◽  
Anna Gallo ◽  
Sara Crotti ◽  
Sara D'Aronco ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Cell Culture ◽  
Culture Systems ◽  
Matrix Interactions ◽  
In Vitro Systems ◽  
Extracellular Matrix Interactions

Three-dimensional (3D) culture systems are progressively getting attention given their potential in overcoming limitations of the classical 2D in vitro systems. Among different supports for 3D cell culture, hydrogels (HGs)...

scholarly journals Skeletal muscle-on-a-chip: an in vitro model to evaluate tissue formation and injury

Lab on a Chip ◽  
2017 ◽  
Vol 17 (20) ◽  
pp. 3447-3461 ◽  
Author(s):  
Gaurav Agrawal ◽  
Aereas Aung ◽  
Shyni Varghese
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Tissue Formation ◽  
Microfluidic Platform ◽  
Muscle Tissues ◽  
Vitro Model

We introduce a microfluidic platform in which we culture three-dimensional skeletal muscle tissues, while evaluating tissue formation and toxin-induced muscle injury.

scholarly journals Primary neuron and astrocyte cultures from postnatal Callithrix jacchus: a non-human primate in vitro model for research in neuroscience, nervous system aging, and neurological diseases of aging

GeroScience ◽  
2020 ◽  
Author(s):  
Angela O. Dorigatti ◽  
Stacy A. Hussong ◽  
Stephen F. Hernandez ◽  
Aubrey M. Sills ◽  
Adam B. Salmon ◽  
...  
Keyword(s):  
Nervous System ◽  
In Vitro Model ◽  
Neurological Diseases ◽  
Callithrix Jacchus ◽  
Primary Neuron ◽  
Vitro Model ◽  
Human Primate

Three-dimensional magnetic resonance imaging for the study of ovarian function in a bovine in vitro model

Reproduction ◽  
2000 ◽  
Vol 119 (1) ◽  
pp. 69-75 ◽  
Author(s):  
G. Sarty ◽  
G. Adams ◽  
R. Pierson
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
In Vitro Model ◽  
Ovarian Function ◽  
Three Dimensional ◽  
Resonance Imaging ◽  
Vitro Model
Sign in / Sign up

Export Citation Format

Share Document