scholarly journals SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jian-An Huang ◽  
Mansoureh Z. Mousavi ◽  
Yingqi Zhao ◽  
Aliaksandr Hubarevich ◽  
Fatima Omeis ◽  
...  
Keyword(s):  
Single Molecule ◽  
Hot Spot ◽  
Surface Enhanced Raman Spectroscopy ◽  
Dna Bases ◽  
Label Free ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Dna Strand ◽  
Sequencing Platforms ◽  
Single Molecule Analysis

AbstractSurface-enhanced Raman spectroscopy (SERS) sensing of DNA bases by plasmonic nanopores could pave a way to novel methods for DNA analyses and new generation single-molecule sequencing platforms. The SERS discrimination of single DNA bases depends critically on the time that a DNA strand resides within the plasmonic hot spot. In fact, DNA molecules flow through the nanopores so rapidly that the SERS signals collected are not sufficient for single-molecule analysis. Here, we report an approach to control the residence time of molecules in the hot spot by an electro-plasmonic trapping effect. By directly adsorbing molecules onto a gold nanoparticle and then trapping the single nanoparticle in a plasmonic nanohole up to several minutes, we demonstrate single-molecule SERS detection of all four DNA bases as well as discrimination of single nucleobases in a single oligonucleotide. Our method can be extended easily to label-free sensing of single-molecule amino acids and proteins.

scholarly journals Application of Aluminum Hydroxide for Improvement of Label-Free SERS Detection of Some Cephalosporin Antibiotics in Urine

Biosensors ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 91 ◽  
Author(s):  
Natalia E. Markina ◽  
Alexey V. Markin
Keyword(s):  
Aluminum Hydroxide ◽  
Surface Enhanced Raman Spectroscopy ◽  
Negative Influence ◽  
Quantitative Detection ◽  
Therapeutic Drug ◽  
Excitation Wavelength ◽  
Sers Substrate ◽  
Label Free ◽  
Surface Enhanced Raman ◽  
Sers Detection

This report is dedicated to development of surface-enhanced Raman spectroscopy (SERS) based analysis protocol for detection of antibiotics in urine. The key step of the protocol is the pretreatment of urine before the detection to minimize background signal. The pretreatment includes extraction of intrinsic urine components using aluminum hydroxide gel (AHG) and further pH adjusting of the purified sample. The protocol was tested by detection of a single antibiotic in artificially spiked samples of real urine. Five antibiotics of cephalosporin class (cefazolin, cefoperazone, cefotaxime, ceftriaxone, and cefuroxime) were used for testing. SERS measurements were performed using a portable Raman spectrometer with 638 nm excitation wavelength and silver nanoparticles as SERS substrate. The calibration curves of four antibiotics (cefuroxime is the exception) cover the concentrations required for detection in patient’s urine during therapy (25/100‒500 μg/mL). Random error of the analysis (RSD < 20%) and limits of quantification (20‒90 μg/mL) for these antibiotics demonstrate the applicability of the protocol for reliable quantitative detection during therapeutic drug monitoring. The detection of cefuroxime using the protocol is not sensitive enough, allowing only for qualitative detection. Additionally, time stability and batch-to-batch reproducibility of AHG were studied and negative influence of the pretreatment protocol and its limitations were estimated and discussed.

scholarly journals Core-Shell Structure of Gold Nanoparticles with Inositol Hexaphosphate Nanohybrids for Label-Free and Rapid Detection by SERS Nanotechnology

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Andreas H. H. Mevold ◽  
Jin-Yuan Liu ◽  
Li-Ying Huang ◽  
Hung-Liang Liao ◽  
Ming-Chien Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Photoelectron Spectroscopy ◽  
Layer Structure ◽  
Hot Spot ◽  
Label Free ◽  
Basic Salt ◽  
Inositol Hexaphosphate ◽  
Vis Spectroscopy ◽  
Surface Enhanced Raman ◽  
Sers Detection

Gold nanoparticles bound with inositol hexaphosphate (IP6) (AuNPs/IP6) were prepared byin situreduction of various concentrations of IP6(0~320 µM) through modified Frens method for surface-enhanced Raman scattering (SERS) detection. The resultant AuNPs/IP6were subject to characterization including UV/Vis spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential, and X-ray photoelectron spectroscopy (XPS). The results showed that AuNPs with 65 µM of IP6would result in a core AuNPs-shell (IP6layer) structure, which exhibited the strongest SERS signal, due to the “hot spot effect” generated from the 1-2 nm interparticle gaps of AuNPs/IP6nanohybrids (ionic interaction of IP6and Au+). Furthermore, the reaction kinetics of Au and IP6were also investigated in this work. Higher concentration of IP6(190 and 260 µM) will make AuNPs become irregularly shaped, because IP6is a basic salt and served as a pH mediator. The morphology and distribution of AuNPs were greatly improved by addition of 65 µM of IP6. This novel AuNPs/IP6nanohybrid showed great stability and Raman enhancement. It is promising in the application of rapid and label-free biological detection of bacteria or tumor cells.

Nanomedical Applications of Optofluidics

2010 ◽  
Author(s):  
David Erickson
Keyword(s):  
Raman Spectroscopy ◽  
Single Molecule ◽  
State Of The Art ◽  
Sensor Arrays ◽  
Surface Enhanced Raman Spectroscopy ◽  
Detection Sensitivity ◽  
Optical Forces ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Single Molecule Analysis

In this talk I will discuss some of the nanomedical applications of optofluidic systems. As will be demonstrated such devices hold significant promise for improving on the state of the art in detection sensitivity as well as enable entirely new modalities for molecular analysis. Two example platforms will be discussed. The first of these will be our Nanoscale Optofluidic Sensor Arrays which comprise of a series of 1D evanescently coupled linear optical resonators. In addition to demonstrating both nucleic acid (Dengue virus) and immunological detection (Interleukins), I will show how optical forces can be used to increase the functionality of these devices including single molecule analysis. The second platform described is a method for performing surface enhanced raman spectroscopy (SERS) on a chip. A unique ligase detection reaction based assay will be demonstrated to show the unique advantages of the approach.

Label-Free Detection of Ovarian Cancer Antigen CA125 by Surface Enhanced Raman Scattering

2020 ◽  
Vol 20 (3) ◽  
pp. 1358-1365 ◽  
Author(s):  
İlknur TunÇ ◽  
Hepi Hari Susapto
Keyword(s):  
Raman Spectroscopy ◽  
Organic Molecules ◽  
Concentration Level ◽  
Hot Spot ◽  
Surface Enhanced Raman Spectroscopy ◽  
Label Free ◽  
Time Resolved ◽  
Label Free Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Surface-enhanced Raman spectroscopy (SERS) has drawn attention in recent years for imaging biologicalmolecules as an analytical tool due to its label-free approach. The SERS approach can be used in tracking organic molecules and monitoring unique Raman spectra of the organic molecules bound to metal nanoparticles (NPs). In this paper, the molecular specifity of Raman Spectroscopy was used together with self-assembled monolayer of metallic AuNPs as a sensor platform in order to detect CA125 antibody-antigen probe molecules. Highly enhanced electromagnetic fields localized around neighboring AuNPs provide hot-spot construction due to the spatial distribution of SERS enhancement on the CA125 proteins at nM concentration level. Time resolved SERS mapping of CA125 antibody and antigen couples was recorded. Even though blinking behavior was observed for some cases, vast variety SERS signals from CA125 proteins were highly reproducible. Blinking behavior is attributed to single molecular detection. Distinguished feature of SERS mapping images of CA125 antibody and antigen with such a low concentration level is very promising for this technique to be used for diagnostic purposes.

scholarly journals Spread spectrum SERS allows label-free detection of attomolar neurotransmitters

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wonkyoung Lee ◽  
Byoung-Hoon Kang ◽  
Hyunwoo Yang ◽  
Moonseong Park ◽  
Ji Hyun Kwak ◽  
...  
Keyword(s):  
Spread Spectrum ◽  
Signal To Noise Ratio ◽  
Low Cost ◽  
Surface Enhanced Raman Spectroscopy ◽  
Experimental Result ◽  
High Temporal Resolution ◽  
Label Free ◽  
Label Free Detection ◽  
Surface Enhanced Raman ◽  
Sers Detection

AbstractThe quantitative label-free detection of neurotransmitters provides critical clues in understanding neurological functions or disorders. However, the identification of neurotransmitters remains challenging for surface-enhanced Raman spectroscopy (SERS) due to the presence of noise. Here, we report spread spectrum SERS (ss-SERS) detection for the rapid quantification of neurotransmitters at the attomolar level by encoding excited light and decoding SERS signals with peak autocorrelation and near-zero cross-correlation. Compared to conventional SERS measurements, the experimental result of ss-SERS shows an exceptional improvement in the signal-to-noise ratio of more than three orders of magnitude, thus achieving a high temporal resolution of over one hundred times. The ss-SERS measurement further allows the attomolar SERS detection of dopamine, serotonin, acetylcholine, γ-aminobutyric acid, and glutamate without Raman reporters. This approach opens up opportunities not only for investigating the early diagnostics of neurological disorders or highly sensitive biomedical SERS applications but also for developing low-cost spectroscopic biosensing applications.

scholarly journals Development and Application of Aptamer-Based Surface-Enhanced Raman Spectroscopy Sensors in Quantitative Analysis and Biotherapy

Sensors ◽  
2019 ◽  
Vol 19 (17) ◽  
pp. 3806 ◽  
Author(s):  
Hai-Xia Wang ◽  
Yu-Wen Zhao ◽  
Zheng Li ◽  
Bo-Shi Liu ◽  
Di Zhang
Keyword(s):  
Surface Enhanced Raman Spectroscopy ◽  
Raman Signal ◽  
Label Free ◽  
Sers Substrates ◽  
Highly Active ◽  
Detection Technology ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Target Molecules

Surface-enhanced Raman scattering (SERS) is one of the most special and important Raman techniques. An apparent Raman signal can be observed when the target molecules are absorbed onto the surface of the SERS substrates, especially on the “hot spots” of the substrates. Early research focused on exploring the highly active SERS substrates and their detection applications in label-free SERS technology. However, it is a great challenge to use these label-free SERS sensors for detecting hydrophobic or non-polar molecules, especially in complex systems or at low concentrations. Therefore, antibodies, aptamers, and antimicrobial peptides have been used to effectively improve the target selectivity and meet the analysis requirements. Among these selective elements, aptamers are easy to use for synthesis and modifications, and their stability, affinity and specificity are extremely good; they have been successfully used in a variety of testing areas. The combination of SERS detection technology and aptamer recognition ability not only improved the selection accuracy of target molecules, but also improved the sensitivity of the analysis. Variations of aptamer-based SERS sensors have been developed and have achieved satisfactory results in the analysis of small molecules, pathogenic microorganism, mycotoxins, tumor marker and other functional molecules, as well as in successful photothermal therapy of tumors. Herein, we present the latest advances of the aptamer-based SERS sensors, as well as the assembling sensing platforms and the strategies for signal amplification. Furthermore, the existing problems and potential trends of the aptamer-based SERS sensors are discussed.

scholarly journals A Versatile DNA Origami-Based Plasmonic Nanoantenna for Label-Free Single-Molecule Surface-Enhanced Raman Spectroscopy

ACS Nano ◽  
2021 ◽  
Vol 15 (4) ◽  
pp. 7065-7077
Author(s):  
Kosti Tapio ◽  
Amr Mostafa ◽  
Yuya Kanehira ◽  
Antonio Suma ◽  
Anushree Dutta ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Single Molecule ◽  
Surface Enhanced Raman Spectroscopy ◽  
Dna Origami ◽  
Label Free ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Molecule Surface

scholarly journals Analysis of Biomolecules Based on the Surface Enhanced Raman Spectroscopy

Nanomaterials ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 730 ◽  
Author(s):  
Min Jia ◽  
Shenmiao Li ◽  
Liguo Zang ◽  
Xiaonan Lu ◽  
Hongyan Zhang
Keyword(s):  
Raman Spectroscopy ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Molecular Structures ◽  
Label Free ◽  
Sers Substrates ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
New Ideas

Analyzing biomolecules is essential for disease diagnostics, food safety inspection, environmental monitoring and pharmaceutical development. Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for detecting biomolecules due to its high sensitivity, rapidness and specificity in identifying molecular structures. This review focuses on the SERS analysis of biomolecules originated from humans, animals, plants and microorganisms, combined with nanomaterials as SERS substrates and nanotags. Recent advances in SERS detection of target molecules were summarized with different detection strategies including label-free and label-mediated types. This comprehensive and critical summary of SERS analysis of biomolecules might help researchers from different scientific backgrounds spark new ideas and proposals.

Toward Label-Free SERS Detection of Proteins through Their Disulfide Bond Structure

2019 ◽  
Vol 25 (1) ◽  
pp. 87-94 ◽  
Author(s):  
Waleed A. Hassanain ◽  
Emad L. Izake
Keyword(s):  
Molecular Structure ◽  
Disulfide Bond ◽  
Disulfide Bonds ◽  
Surface Enhanced Raman Spectroscopy ◽  
Label Free ◽  
Ultralow Concentrations ◽  
Relative Standard ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Oxide Substrate

The molecular structure of many proteins contains disulfide bonds between their cysteine residues. In this work we demonstrate the utilization of the disulfide bond structure of proteins for their label-free determination by surface-enhanced Raman spectroscopy (SERS). The new approach for label-free SERS detection of proteins is demonstrated for human insulin. The protein was selectively extracted from spiked plasma samples using target-specific functionalized nanomaterial. Enzyme-linked immune assay (ELISA) was used to detect insulin in the blood plasma and cross-validate the SERS method. The disulfide bonds in the molecular structure of the protein were chemically reduced and used for their chemisorption onto the gold-coated copper oxide substrate in a unified orientation at a very short distance from the hotspots. The oriented chemisorption of the protein caused significant enhancement to the signal intensity of its Raman vibration modes. This is attributed to the strong short-range electromagnetic and chemical enhancement effects that are experienced by the immobilized protein. Using this approach, label-free and reproducible SERS detection of insulin, down to 10 zM (relative standard deviation [RSD] = 5.52%), was achieved. Sixty-five percent of proteins contain disulfide bonds in their molecular structure. Therefore, the new label-free SERS detection method has strong potential for the determination of ultralow concentrations of proteins at pathology labs and in biology research.

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