scholarly journals Application of Aluminum Hydroxide for Improvement of Label-Free SERS Detection of Some Cephalosporin Antibiotics in Urine

Biosensors ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 91 ◽  
Author(s):  
Natalia E. Markina ◽  
Alexey V. Markin
Keyword(s):  
Aluminum Hydroxide ◽  
Surface Enhanced Raman Spectroscopy ◽  
Negative Influence ◽  
Quantitative Detection ◽  
Therapeutic Drug ◽  
Excitation Wavelength ◽  
Sers Substrate ◽  
Label Free ◽  
Surface Enhanced Raman ◽  
Sers Detection

This report is dedicated to development of surface-enhanced Raman spectroscopy (SERS) based analysis protocol for detection of antibiotics in urine. The key step of the protocol is the pretreatment of urine before the detection to minimize background signal. The pretreatment includes extraction of intrinsic urine components using aluminum hydroxide gel (AHG) and further pH adjusting of the purified sample. The protocol was tested by detection of a single antibiotic in artificially spiked samples of real urine. Five antibiotics of cephalosporin class (cefazolin, cefoperazone, cefotaxime, ceftriaxone, and cefuroxime) were used for testing. SERS measurements were performed using a portable Raman spectrometer with 638 nm excitation wavelength and silver nanoparticles as SERS substrate. The calibration curves of four antibiotics (cefuroxime is the exception) cover the concentrations required for detection in patient’s urine during therapy (25/100‒500 μg/mL). Random error of the analysis (RSD < 20%) and limits of quantification (20‒90 μg/mL) for these antibiotics demonstrate the applicability of the protocol for reliable quantitative detection during therapeutic drug monitoring. The detection of cefuroxime using the protocol is not sensitive enough, allowing only for qualitative detection. Additionally, time stability and batch-to-batch reproducibility of AHG were studied and negative influence of the pretreatment protocol and its limitations were estimated and discussed.

Multivariate Analysis Aided Surface-Enhanced Raman Spectroscopy (MVA-SERS) Multiplex Quantitative Detection of Trace Fentanyl in Illicit Drug Mixtures Using a Handheld Raman Spectrometer

2021 ◽  
pp. 000370282110329
Author(s):  
Ling Wang ◽  
Mario O. Vendrell-Dones ◽  
Chiara Deriu ◽  
Sevde Doğruer ◽  
Peter de B. Harrington ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Principal Component ◽  
Surface Enhanced Raman Spectroscopy ◽  
Quantitative Detection ◽  
Least Squares Regression ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Current Screening

Recently there has been upsurge in reports that illicit seizures of cocaine and heroin have been adulterated with fentanyl. Surface-enhanced Raman spectroscopy (SERS) provides a useful alternative to current screening procedures that permits detection of trace levels of fentanyl in mixtures. Samples are solubilized and allowed to interact with aggregated colloidal nanostars to produce a rapid and sensitive assay. In this study, we present the quantitative determination of fentanyl in heroin and cocaine using SERS, using a point-and-shoot handheld Raman system. Our protocol is optimized to detect pure fentanyl down to 0.20 ± 0.06 ng/mL and can also distinguish pure cocaine and heroin at ng/mL levels. Multiplex analysis of mixtures is enabled by combining SERS detection with principal component analysis and super partial least squares regression discriminate analysis (SPLS-DA), which allow for the determination of fentanyl as low as 0.05% in simulated seized heroin and 0.10% in simulated seized cocaine samples.

scholarly journals Design of Inverted Nano-Cone Arrayed SERS Substrate for Rapid Detection of Pathogens

2021 ◽  
Vol 11 (17) ◽  
pp. 8067
Author(s):  
Zixun Jia ◽  
Sarah Asiri ◽  
Asma Elsharif ◽  
Widyan Alamoudi ◽  
Ebtesam Al-Suhaimi ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Rapid Detection ◽  
Close Contact ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Substrate ◽  
Label Free ◽  
Surface Enhanced Raman ◽  
Order Of Magnitude ◽  
The Right ◽  
Pathogen Diagnosis

Rapid detection of bacteria is a very critical and important part of infectious disease treatment. Sepsis kills more than 25 percent of its victims, resulting in as many as half of all deaths in hospitals before identifying the pathogen for patients to get the right treatment. Raman spectroscopy is a promising candidate in pathogen diagnosis given its fast and label-free nature, only if the concentration of the pathogen is high enough to provide reasonable sensitivity. This work reports a new design of surface-enhanced Raman spectroscopy (SERS) substrate which will provide high enough sensitivity and fast and close contact of the target structure to the optical hot spots for immunomagnetic capturing-based bacteria-concentrating technique. The substrate uses inverted nanocone structure arrays made of transparent PDMS (Polydimethylsiloxane) to funnel the light from the bottom to the top of the cones where plasmonic gold nanorods are located. A high reflective and low loss layer is deposited on the outer surface of the cone. Given the geometry of cones, photons are multi-reflected by the outer layer and thus the number density of photons at hotspots increases by an order of magnitude, which could be high enough to detect immunomagnetically densified bacteria.

scholarly journals Flexible and Reusable Ag Coated TiO2 Nanotube Arrays for Highly Sensitive SERS Detection of Formaldehyde

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1199 ◽  
Author(s):  
Tong Zhu ◽  
Hang Wang ◽  
Libin Zang ◽  
Sila Jin ◽  
Shuang Guo ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Catalytic Performance ◽  
Tio2 Nanotube ◽  
Detection Sensitivity ◽  
Sers Substrate ◽  
Environmental Media ◽  
Sensing Platform ◽  
Surface Enhanced Raman ◽  
Sers Detection

Quantitative analysis of formaldehyde (HCHO, FA), especially at low levels, in various environmental media is of great importance for assessing related environmental and human health risks. A highly efficient and convenient FA detection method based on surface-enhanced Raman spectroscopy (SERS) technology has been developed. This SERS-based method employs a reusable and soft silver-coated TiO2 nanotube array (TNA) material, such as an SERS substrate, which can be used as both a sensing platform and a degradation platform. The Ag-coated TNA exhibits superior detection sensitivity with high reproducibility and stability compared with other SERS substrates. The detection of FA is achieved using the well-known redox reaction of FA with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (AHMT) at room temperature. The limit of detection (LOD) for FA is 1.21 × 10−7 M. In addition, the stable catalytic performance of the array allows the degradation and cleaning of the AHMT-FA products adsorbed on the array surface under ultraviolet irradiation, making this material recyclable. This SERS platform displays a real-time monitoring platform that combines the detection and degradation of FA.

scholarly journals Detection of a Sudan dye at low concentrations by surface-enhanced Raman spectroscopy using silver nanoparticles

2019 ◽  
Vol 29 (4) ◽  
pp. 521
Author(s):  
Tran Cao Dao ◽  
Truc Quynh Ngan Luong ◽  
Tuan Anh Cao ◽  
Ngoc Minh Kieu
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Good Method ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Substrate ◽  
Sudan Dyes ◽  
Sudan I ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection

Sudan dyes are red colorants banned from use for food due to their toxic properties. However, because of the cheapness, they are sometimes adulterated into food illegally. Currently surface-enhanced Raman spectroscopy (SERS) is emerging as a good method to detect residues (including trace amounts) of Sudan dyes in food. In this report we present the SERS detection of Sudan I (a type of Sudan dyes) to concentrations as low as 1 ppb, using a very simple SERS substrate, which is made from silver nanoparticles chemically deposited on silicon surface.

scholarly journals Spread spectrum SERS allows label-free detection of attomolar neurotransmitters

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wonkyoung Lee ◽  
Byoung-Hoon Kang ◽  
Hyunwoo Yang ◽  
Moonseong Park ◽  
Ji Hyun Kwak ◽  
...  
Keyword(s):  
Spread Spectrum ◽  
Signal To Noise Ratio ◽  
Low Cost ◽  
Surface Enhanced Raman Spectroscopy ◽  
Experimental Result ◽  
High Temporal Resolution ◽  
Label Free ◽  
Label Free Detection ◽  
Surface Enhanced Raman ◽  
Sers Detection

AbstractThe quantitative label-free detection of neurotransmitters provides critical clues in understanding neurological functions or disorders. However, the identification of neurotransmitters remains challenging for surface-enhanced Raman spectroscopy (SERS) due to the presence of noise. Here, we report spread spectrum SERS (ss-SERS) detection for the rapid quantification of neurotransmitters at the attomolar level by encoding excited light and decoding SERS signals with peak autocorrelation and near-zero cross-correlation. Compared to conventional SERS measurements, the experimental result of ss-SERS shows an exceptional improvement in the signal-to-noise ratio of more than three orders of magnitude, thus achieving a high temporal resolution of over one hundred times. The ss-SERS measurement further allows the attomolar SERS detection of dopamine, serotonin, acetylcholine, γ-aminobutyric acid, and glutamate without Raman reporters. This approach opens up opportunities not only for investigating the early diagnostics of neurological disorders or highly sensitive biomedical SERS applications but also for developing low-cost spectroscopic biosensing applications.

scholarly journals Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3212 ◽  
Author(s):  
Zdeněk Pilát ◽  
Martin Kizovský ◽  
Jan Ježek ◽  
Stanislav Krátký ◽  
Jaroslav Sobota ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Gold Surface ◽  
Surface Enhanced Raman Spectroscopy ◽  
Microfluidic System ◽  
Environmental Pollutant ◽  
Spectral Lines ◽  
Quantitative Detection ◽  
Sers Substrate ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples, e.g., for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we have developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

scholarly journals Development and Application of Aptamer-Based Surface-Enhanced Raman Spectroscopy Sensors in Quantitative Analysis and Biotherapy

Sensors ◽  
2019 ◽  
Vol 19 (17) ◽  
pp. 3806 ◽  
Author(s):  
Hai-Xia Wang ◽  
Yu-Wen Zhao ◽  
Zheng Li ◽  
Bo-Shi Liu ◽  
Di Zhang
Keyword(s):  
Surface Enhanced Raman Spectroscopy ◽  
Raman Signal ◽  
Label Free ◽  
Sers Substrates ◽  
Highly Active ◽  
Detection Technology ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Target Molecules

Surface-enhanced Raman scattering (SERS) is one of the most special and important Raman techniques. An apparent Raman signal can be observed when the target molecules are absorbed onto the surface of the SERS substrates, especially on the “hot spots” of the substrates. Early research focused on exploring the highly active SERS substrates and their detection applications in label-free SERS technology. However, it is a great challenge to use these label-free SERS sensors for detecting hydrophobic or non-polar molecules, especially in complex systems or at low concentrations. Therefore, antibodies, aptamers, and antimicrobial peptides have been used to effectively improve the target selectivity and meet the analysis requirements. Among these selective elements, aptamers are easy to use for synthesis and modifications, and their stability, affinity and specificity are extremely good; they have been successfully used in a variety of testing areas. The combination of SERS detection technology and aptamer recognition ability not only improved the selection accuracy of target molecules, but also improved the sensitivity of the analysis. Variations of aptamer-based SERS sensors have been developed and have achieved satisfactory results in the analysis of small molecules, pathogenic microorganism, mycotoxins, tumor marker and other functional molecules, as well as in successful photothermal therapy of tumors. Herein, we present the latest advances of the aptamer-based SERS sensors, as well as the assembling sensing platforms and the strategies for signal amplification. Furthermore, the existing problems and potential trends of the aptamer-based SERS sensors are discussed.

scholarly journals Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chip

2018 ◽  
Author(s):  
Zdeněk Pilát ◽  
Martin Kizovský ◽  
Jan Ježek ◽  
Stanislav Krátký ◽  
Jaroslav Sobota ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Gold Surface ◽  
Surface Enhanced Raman Spectroscopy ◽  
Microfluidic System ◽  
Environmental Pollutant ◽  
Spectral Lines ◽  
Quantitative Detection ◽  
Sers Substrate ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

scholarly journals Plasmonic Core–Shell–Satellites with Abundant Electromagnetic Hotspots for Highly Sensitive and Reproducible SERS Detection

2021 ◽  
Vol 22 (22) ◽  
pp. 12191
Author(s):  
Puran Pandey ◽  
Sundar Kunwar ◽  
Ki-Hoon Shin ◽  
Min-Kyu Seo ◽  
Jongwon Yoon ◽  
...  
Keyword(s):  
High Surface ◽  
Atomic Layer ◽  
Surface Enhanced Raman Spectroscopy ◽  
Core Shell ◽  
Sers Substrate ◽  
Practical Applications ◽  
Highly Sensitive ◽  
Relative Standard ◽  
Surface Enhanced Raman ◽  
Sers Detection

In this work, we develop a Ag@Al2O3@Ag plasmonic core–shell–satellite (PCSS) to achieve highly sensitive and reproducible surface-enhanced Raman spectroscopy (SERS) detection of probe molecules. To fabricate PCSS nanostructures, we employ a simple hierarchical dewetting process of Ag films coupled with an atomic layer deposition (ALD) method for the Al2O3 shell. Compared to bare Ag nanoparticles, several advantages of fabricating PCSS nanostructures are discovered, including high surface roughness, high density of nanogaps between Ag core and Ag satellites, and nanogaps between adjacent Ag satellites. Finite-difference time-domain (FDTD) simulations of the PCSS nanostructure confirm an enhancement in the electromagnetic field intensity (hotspots) in the nanogap between the Ag core and the satellite generated by the Al2O3 shell, due to the strong core–satellite plasmonic coupling. The as-prepared PCSS-based SERS substrate demonstrates an enhancement factor (EF) of 1.7 × 107 and relative standard deviation (RSD) of ~7%, endowing our SERS platform with highly sensitive and reproducible detection of R6G molecules. We think that this method provides a simple approach for the fabrication of PCSS by a solid-state technique and a basis for developing a highly SERS-active substrate for practical applications.

scholarly journals SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jian-An Huang ◽  
Mansoureh Z. Mousavi ◽  
Yingqi Zhao ◽  
Aliaksandr Hubarevich ◽  
Fatima Omeis ◽  
...  
Keyword(s):  
Single Molecule ◽  
Hot Spot ◽  
Surface Enhanced Raman Spectroscopy ◽  
Dna Bases ◽  
Label Free ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Dna Strand ◽  
Sequencing Platforms ◽  
Single Molecule Analysis

AbstractSurface-enhanced Raman spectroscopy (SERS) sensing of DNA bases by plasmonic nanopores could pave a way to novel methods for DNA analyses and new generation single-molecule sequencing platforms. The SERS discrimination of single DNA bases depends critically on the time that a DNA strand resides within the plasmonic hot spot. In fact, DNA molecules flow through the nanopores so rapidly that the SERS signals collected are not sufficient for single-molecule analysis. Here, we report an approach to control the residence time of molecules in the hot spot by an electro-plasmonic trapping effect. By directly adsorbing molecules onto a gold nanoparticle and then trapping the single nanoparticle in a plasmonic nanohole up to several minutes, we demonstrate single-molecule SERS detection of all four DNA bases as well as discrimination of single nucleobases in a single oligonucleotide. Our method can be extended easily to label-free sensing of single-molecule amino acids and proteins.

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