scholarly journals Photoresponsive supramolecular coordination polyelectrolyte as smart anticounterfeiting inks

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhiqiang Li ◽  
Xiao Liu ◽  
Guannan Wang ◽  
Bin Li ◽  
Hongzhong Chen ◽  
...  
Keyword(s):  
Self Assembly ◽  
Ring Opening ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Ring Closure ◽  
New Class ◽  
Supramolecular Coordination ◽  
Ligand Coordination ◽  
Information Patterns ◽  
New Generation

AbstractWhile photoluminescence printing is a widely applied anticounterfeiting technique, there are still challenges in developing new generation anticounterfeiting materials with high security. Here we report the construction of a photoresponsive supramolecular coordination polyelectrolyte (SCP) through hierarchical self-assembly of lanthanide ion, bis-ligand and diarylethene unit, driven by metal-ligand coordination and ionic interaction. Owing to the conformation-dependent photochromic fluorescence resonance energy transfer between the lanthanide donor and diarylethene acceptor, the ring-closure/ring-opening isomerization of the diarylethene unit leads to a photoreversible luminescence on/off switch in the SCP. The SCP is then utilized as security ink to print various patterns, through which photoreversible multiple information patterns with visible/invisible transformations are realized by simply alternating the irradiation with UV and visible light. This work demonstrates the possibility of developing a new class of smart anticounterfeiting materials, which could be operated in a noninvasive manner with a higher level of security.

Programmable in vitro Co-Encapsidation of Guest Proteins Inside Protein Nanocages

2018 ◽  
Author(s):  
Noor H. Dashti ◽  
Rufika S. Abidin ◽  
Frank Sainsbury
Keyword(s):  
Self Assembly ◽  
Mammalian Cells ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Super Resolution ◽  
Cell Engineering ◽  
Particle Analysis ◽  
Protein Cages

Bioinspired self-sorting and self-assembling systems using engineered versions of natural protein cages have been developed for biocatalysis and therapeutic delivery. The packaging and intracellular delivery of guest proteins is of particular interest for both <i>in vitro</i> and <i>in vivo</i> cell engineering. However, there is a lack of platforms in bionanotechnology that combine programmable guest protein encapsidation with efficient intracellular uptake. We report a minimal peptide anchor for <i>in vivo</i> self-sorting of cargo-linked capsomeres of the Murine polyomavirus (MPyV) major coat protein that enables controlled encapsidation of guest proteins by <i>in vitro</i> self-assembly. Using Förster resonance energy transfer (FRET) we demonstrate the flexibility in this system to support co-encapsidation of multiple proteins. Complementing these ensemble measurements with single particle analysis by super-resolution microscopy shows that the stochastic nature of co-encapsidation is an overriding principle. This has implications for the design and deployment of both native and engineered self-sorting encapsulation systems and for the assembly of infectious virions. Taking advantage of the encoded affinity for sialic acids ubiquitously displayed on the surface of mammalian cells, we demonstrate the ability of self-assembled MPyV virus-like particles to mediate efficient delivery of guest proteins to the cytosol of primary human cells. This platform for programmable co-encapsidation and efficient cytosolic delivery of complementary biomolecules therefore has enormous potential in cell engineering.

scholarly journals A novel quantification platform for point-of-care testing of circulating MicroRNAs based on allosteric spherical nanoprobe

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Zhi Li ◽  
Ke Xia ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer (NSCLC), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC. Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield “signal on” fluorescence. In the manner of multipoint fluorescence detection, the target-bound allosteric spherical nanoprobe could provide high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method can distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

Comparison of self-assembled and micelle encapsulated QD chemosensor constructs for biological sensing

2015 ◽  
Vol 185 ◽  
pp. 249-266 ◽  
Author(s):  
Christopher M. Lemon ◽  
Daniel G. Nocera
Keyword(s):  
Self Assembly ◽  
Metabolic Profiling ◽  
Photophysical Properties ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Core Shell ◽  
Biological Sensing ◽  
Two Photon ◽  
Photon Excitation ◽  
Fret Efficiency

Whereas a variety of covalent conjugation strategies have been utilized to prepare quantum dot (QD)-based nanosensors, supramolecular approaches of self-assembly have been underexplored. A major advantage of self-assembly is the ability to circumvent laborious synthetic efforts attendant to covalent conjugation of a chemosensor to functionalized QDs. Here, we combine a CdSe/ZnS core–shell QD with gold(iii) corroles using both self-assembly and micelle encapsulation to form QD nanosensors. Appreciable spectral overlap between QD emission and corrole absorption results in efficient Förster resonance energy transfer (FRET), which may be initiated by one- or two-photon excitation. The triplet state of the gold(iii) corroles is quenched by molecular oxygen, enabling these constructs to function as optical O2 sensors, which is useful for the metabolic profiling of tumours. The photophysical properties, including QD and corrole lifetimes, FRET efficiency, and O2 sensitivity, have been determined for each construct. The relative merits of each conjugation strategy are assessed with regard to their implementation as sensors.

Spontaneous Self-Assembly, Functionalization, and Meso-Scale Host-Guest Science of Organic Nanotubes

2007 ◽  
Vol 1061 ◽  
Author(s):  
Naohiro Kameta ◽  
Mitsutoshi Masuda ◽  
Toshimi Shimizu
Keyword(s):  
Hollow Cylinder ◽  
Self Assembly ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Recognition System ◽  
Covalent Modification ◽  
Amino Groups ◽  
Guest Molecules ◽  
Inner Surface ◽  
Organic Nanotubes

ABSTRACTSelf-assembly in water at pH 6 and 10 of a wedge-shaped bolaamphiphile (1), bearing amino and glucose hydrophilic headgroups, was found to give two types of nanotubes with different inner diameters of 20 and 80 nm, respectively. X-ray diffraction and IR measurement revealed that the nanotubes have distinct inner and outer surfaces covered with amino and glucose headgroups, respectively. The nanotubes were able to effectively encapsulate an anionic spherical protein, ferritin, and DNA into the hollow cylinder under neutral pH conditions. Positively charged amino groups in the nanotube hollow cylinder proved to accelerate the encapsulation of the anionic biomacromolecules via electrostatic interaction. Covalent modification of the amino groups with a fluorescent donor dye on the inner surface of the nanotube allowed us to achieve the construction of an optical recognition system for encapsulation of guest molecules. Fluorescence resonance energy transfer (FRET) from the fluorescent donor located on the inner surface to the ferritin labeled with fluorescent acceptor enabled us to visualize the encapsulation and transportation features of the ferritin in the nanochannel shaped by the hollow cylinder structure.

scholarly journals A Novel Quantification Platform for Point-of-care testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

2020 ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Zhi Li ◽  
Ke Xia ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer ( NSCLC ), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC . Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield a “signal on” fluorescence. In the manner of multipoint fluorescence detection , the target-bound allosteric spherical nanoprobe could provide a high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method could distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

scholarly journals Endogenous microRNA Triggered Enzyme-free DNA Logic Self-assembly for Amplified Bioimaging and Enhanced Gene Therapy via in Situ Generation of siRNAs

2021 ◽  
Author(s):  
Qinghua Jiang ◽  
Shuzhen Yue ◽  
Kaixin Yu ◽  
Tian Tian ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Self Assembly ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Amplification ◽  
High Sensitivity ◽  
Free Dna ◽  
Therapy Effect ◽  
In Situ Generation

Abstract BackgroundSmall interfering RNA (siRNA) has emerged as a kind of promising therapeutic agents for cancer therapy. However, the off-target effect and degradation are the main challenges for siRNAs delivery. Herein, an enzyme-free DNA amplification strategy initiated by a specific endogenous microRNA has been developed for in situ generation of siRNAs with enhanced gene therapy effect on cervical carcinoma.MethodsThis strategy contains three DNA hairpins (H1, H2/PS and H3) which can be triggered by microRNA-21 (miR-21) for self-assembly of DNA nanowheels (DNWs). Notably, this system is consistent with the operation of a DNA logic circuitry containing cascaded “AND” gates with feedback mechanism. Accordingly, a versatile biosensing and bioimaging platform is fabricated for sensitive and specific analysis of miR-21 in HeLa cells via fluorescence resonance energy transfer (FRET). Meanwhile, since the vascular endothelial growth factor (VEGF) antisense and sense sequences are encoded in hairpin reactants, the performance of this DNA circuit leads to in situ assembly of VEGF siRNAs in DNWs, which can be specifically recognized and cleaved by Dicer for gene therapy of cervical carcinoma. ResultsThe proposed isothermal amplification approach exhibits high sensitivity for miR-21 with a detection limit of 0.25 pM and indicates excellent specificity to discriminate target miR-21 from the single-base mismatched sequence. Furthermore, this strategy achieves accurate and sensitive imaging analysis of the expression and distribution of miR-21 in different living cells. To note, compared to naked siRNAs alone, in situ siRNA generation shows a significantly enhanced gene silencing and anti-tumor effect due to the high reaction efficiency of DNA circuit and improved delivery stability of siRNAs.ConclusionThe endogenous miRNA-activated DNA circuit provides an exciting opportunity to construct a general nanoplatform for precise cancer diagnosis and efficient gene therapy, which has an important significance in clinical translation.

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