scholarly journals Rapid discovery of self-assembling peptides with one-bead one-compound peptide library

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pei-Pei Yang ◽  
Yi-Jing Li ◽  
Yan Cao ◽  
Lu Zhang ◽  
Jia-Qi Wang ◽  
...  
Keyword(s):  
Combinatorial Library ◽  
Rapid Identification ◽  
Screening Strategy ◽  
Peptide Library ◽  
Aqueous Environment ◽  
Self Assembling ◽  
Material Sciences ◽  
The One ◽  
Combinatorial Space ◽  
Simple Screening

AbstractSelf-assembling peptides have shown tremendous potential in the fields of material sciences, nanoscience, and medicine. Because of the vast combinatorial space of even short peptides, identification of self-assembling sequences remains a challenge. Herein, we develop an experimental method to rapidly screen a huge array of peptide sequences for self-assembling property, using the one-bead one-compound (OBOC) combinatorial library method. In this approach, peptides on beads are N-terminally capped with nitro-1,2,3-benzoxadiazole, a hydrophobicity-sensitive fluorescence molecule. Beads displaying self-assembling peptides would fluoresce under aqueous environment. Using this approach, we identify eight pentapeptides, all of which are able to self-assemble into nanoparticles or nanofibers. Some of them are able to interact with and are taken up efficiently by HeLa cells. Intracellular distribution varied among these non-toxic peptidic nanoparticles. This simple screening strategy has enabled rapid identification of self-assembling peptides suitable for the development of nanostructures for various biomedical and material applications.

scholarly journals Rapid Discovery of Self-Assembling Peptides with One-Bead One-Compound Peptide Libraries

2020 ◽  
Author(s):  
Peipei Yang ◽  
Yi-Jing Li ◽  
Yan Cao ◽  
Lu Zhang ◽  
Jia-Qi Wang ◽  
...  
Keyword(s):  
Combinatorial Library ◽  
Rapid Identification ◽  
Screening Strategy ◽  
Aqueous Environment ◽  
Self Assembling ◽  
Material Sciences ◽  
The One ◽  
Combinatorial Space ◽  
Simple Screening ◽  
Library Method

Abstract Self-assembling peptides have shown tremendous potential in the fields of material sciences, nanoscience, and medicine. Because of the vast combinatorial space of even short peptides, identification of self-assembling sequences remains a challenge. Herein, we develop an experimental method to rapidly screen a huge array of peptide sequences for self-assembling property, using the one-bead one-compound (OBOC) combinatorial library method. In this approach, peptides on beads are N-terminally capped with nitro-1,2,3-benzoxadiazole, a hydrophobicity-sensitive fluorescence molecule. Beads displaying self-assembling peptides would fluoresce under aqueous environment. Using this approach, we identified eight pentapeptides, all of which were able to self-assemble into nanoparticles or nanofibers. Some of them were able to interact with and taken up efficiently by HeLa cells. Intracellular distribution varied among these non-toxic peptidic nanoparticles. This novel but very simple screening strategy, has enabled rapid identification of self-assembling peptides suitable for the development of nanostructures for various biomedical and material applications.

A SIMPLE SCREENING STRATEGY FOR DIAGNOSING THE PORPHYRIAS

1979 ◽  
Vol 2 (9) ◽  
pp. 474-477 ◽  
Author(s):  
ROY W. PAIN ◽  
PATRICK J. PHILLIPS ◽  
HUGH A. J. HARLEY ◽  
JAMES H. BEARE
Keyword(s):  
Screening Strategy ◽  
Simple Screening

Rapid Identification of Substrates for Novel Proteases Using a Combinatorial Peptide Library

2000 ◽  
Vol 2 (5) ◽  
pp. 461-466 ◽  
Author(s):  
Gérard Rossé ◽  
Erich Kueng ◽  
Malcolm G. P. Page ◽  
Vesna Schauer-Vukasinovic ◽  
Thomas Giller ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Peptide Library ◽  
Combinatorial Peptide Library

Study on Fuzzy Cluster in the Model for Rapid Identification of NC Processing

2012 ◽  
Vol 522 ◽  
pp. 673-676
Author(s):  
Xi Li ◽  
Dan Feng Feng
Keyword(s):  
Experimental Study ◽  
Intelligent Control ◽  
Likelihood Function ◽  
Rapid Identification ◽  
Fuzzy Cluster ◽  
Identification Algorithm ◽  
Signal Identification ◽  
Dynamic Identification ◽  
The One ◽  
S Model

In this paper, a application of signal identification by using fuzzy cluster is studied Based on the one order T-S model, an algorithm for online establishment the nonlinear model between the servo current and cutting force is presented by fuzzy likelihood function to derive fuzzy cluster. Finally, the experimental study has been given. The result showed that it can be regarded as a good dynamic identification algorithm for intelligent control of NC processing.

scholarly journals Screening a library of FDA-approved and bioactive compounds for antiviral activity against SARS-CoV-2

2020 ◽  
Author(s):  
Scott B. Biering ◽  
Erik Van Dis ◽  
Eddie Wehri ◽  
Livia H. Yamashiro ◽  
Xammy Nguyenla ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Combination Therapy ◽  
Global Health ◽  
Antiviral Activity ◽  
Drug Repurposing ◽  
Rapid Identification ◽  
Screening Strategy ◽  
Combination Therapies ◽  
Pulmonary Epithelial Cells ◽  
Compound Screening

AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), has emerged as a major global health threat. The COVID-19 pandemic has resulted in over 80 million cases and 1.7 million deaths to date while the number of cases continues to rise. With limited therapeutic options, the identification of safe and effective therapeutics is urgently needed. The repurposing of known clinical compounds holds the potential for rapid identification of drugs effective against SARS-CoV-2. Here we utilized a library of FDA-approved and well-studied preclinical and clinical compounds to screen for antivirals against SARS-CoV-2 in human pulmonary epithelial cells. We identified 13 compounds that exhibit potent antiviral activity across multiple orthogonal assays. Hits include known antivirals, compounds with anti-inflammatory activity, and compounds targeting host pathways such as kinases and proteases critical for SARS-CoV-2 replication. We identified seven compounds not previously reported to have activity against SARS-CoV-2, including B02, a human RAD51 inhibitor. We further demonstrated that B02 exhibits synergy with remdesivir, the only antiviral approved by the FDA to treat COVID-19, highlighting the potential for combination therapy. Taken together, our comparative compound screening strategy highlights the potential of drug repurposing screens to identify novel starting points for development of effective antiviral mono- or combination therapies to treat COVID-19.

The Features of Investigations and Observations of Nanoamorphous Metals and Nanocrystalline Oxides on Electron Microscope

2004 ◽  
Vol 839 ◽  
Author(s):  
R. Malkhasyan ◽  
R. Karakhanyan ◽  
M. Nazaryan ◽  
A. Khachatryan ◽  
A. Markosyan
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Local Heating ◽  
Fine Sand ◽  
Inorganic Particles ◽  
Self Assembling ◽  
Small Crystals ◽  
Precautionary Measures ◽  
The One ◽  
Nanocrystalline Oxides

ABSTRACTThe present paper discusses two different active influences of an electron beam on a nanosize sample.The first observed influence is the formation of nanoparticle agglomerates due to the beam charge. This can be likened to the formation of fine sand and it enables the determination of grain magnitude of the given nanomaterial. This also enables to get a clear picture of the sample, which is latent at first due to the coating of nanoparticles and their agglomerates. For example, removal of nanopowder coatings has enabled for the first time to find self - assembling systems in a shape of double chain spatial helixes from inorganic particles (as DNA).The second type of influence is related to transformations, which distort the sample. Transformation of amorphous particles of metal into a crystalline state by means of local heating is the one of most importance.Growth of thread-shaped hollow and solid nanowhiskers from nanoparticles and their further disintegration to a great number of small crystals under influence of electron beam in the chamber of electron microscope was observed. Therefore it can be confirmed that it is necessary to use an electron microscope with special precautionary measures, when working with nanoamorphous metals.

ChemInform Abstract: The “One-Bead-One-Compound” Combinatorial Library Method

ChemInform ◽  
2010 ◽  
Vol 28 (27) ◽  
pp. no-no
Author(s):  
K. S. LAM ◽  
M. LEBL ◽  
V. KRCHNAK
Keyword(s):  
Combinatorial Library ◽  
The One ◽  
Library Method

scholarly journals Warehouse design: a systematic literature review

2017 ◽  
Vol 14 (4) ◽  
pp. 542 ◽  
Author(s):  
Augusto Da Cunha Reis ◽  
Cristina Gomes de Souza ◽  
Nayara Nogueira da Costa ◽  
Gustavo Henrique Cordeiro Stender ◽  
Pedro Senna Vieira ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Qualitative Approach ◽  
Three Dimensions ◽  
Review Of The Literature ◽  
Future Studies ◽  
Academic Literature ◽  
Design And Implementation ◽  
Warehouse Design ◽  
The One ◽  
Initial Research

Abstract: This study proposes improving the understanding of the main aspects involved in the design of warehouses by the construction of a framework that reveals the state-of-art.  The initial research bibliography generated a framework, which was structured in three dimensions: inputs; design and implementation; and outputs. The validation of framework was accomplished through a systematic review of the literature, covering 68 articles published in the period 1999- 2015. This study covered the main aspects highlighted in the academic literature that influence the design of warehouses. Additionally, an overview of the publications based on a theoretical/empirical and a quantitative/qualitative approach was pointed out. This paper aims to contribute to both industry and academic. On the one hand, the framework aggregates value for professionals by permitting the rapid identification of variables, which must be considered in warehouse design. On the other hand, by systematizing the warehouse design area, researchers are able to identify gaps that may generate future studies. 

Sign in / Sign up

Export Citation Format

Share Document