Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet

ABSTRACTCold atmospheric pressure plasma has been intensively studied due to growing interest in biomedical applications. For example, it has been revealed that the plasma-irradiated cell culture medium (PAM: Plasma Activated Medium) shows antitumor effect as well as the direct plasma treatment. Long-lived reactive oxygen and nitrogen species (RONS), such as hydrogen peroxide (H2O2), nitrite (NO2-), and nitrate (NO3-), are considered as a main product in PAM. Therefore, investigation of the production characteristics of RONS in PAM with different experimental parameters is important. Here, we focused on humidification of feed gas (argon or helium) and effect of gap between the nozzle of the plasma jet and liquid surface. As a result, H2O2 concentration was increased by humidification of both feed gases. However, the concentration of NO2- and NO3- was decreased by the humidification of helium. In addition, the remarkable effect of the humidification on H2O2 production was observed when the plasma jet was in contact with the liquid surface.

Download Full-text

On the penetration of reactive oxygen and nitrogen species generated by a plasma jet into and through mice skin with/without stratum corneum

Physics of Plasmas ◽

10.1063/1.5082160 ◽

2019 ◽

Vol 26 (4) ◽

pp. 043504 ◽

Cited By ~ 11

Author(s):

J. Duan ◽

L. Gan ◽

L. Nie ◽

F. Sun ◽

X. Lu ◽

...

Keyword(s):

Stratum Corneum ◽

Reactive Oxygen ◽

Plasma Jet ◽

Nitrogen Species

Download Full-text

A Synergistic Effect of Reactive Oxygen and Reactive Nitrogen Species in Plasma Activated Liquid Media Triggers Astrocyte Wound Healing

International Journal of Molecular Sciences ◽

10.3390/ijms21093343 ◽

2020 ◽

Vol 21 (9) ◽

pp. 3343

Author(s):

Eloisa Sardella ◽

Maria Grazia Mola ◽

Roberto Gristina ◽

Monica Piccione ◽

Valeria Veronico ◽

...

Keyword(s):

Wound Healing ◽

Cell Growth ◽

Connexin 43 ◽

Inflammatory Marker ◽

Reactive Oxygen ◽

Active Species ◽

Nitrogen Species ◽

Liquid Media ◽

Negative Effect ◽

And Migration

Astrocyte proliferation and migration toward injured Central Nervous System (CNS) areas are key features of astrogliosis and glial scar formation. Even though it is known that intracellular and environmental Reactive Oxygen and Nitrogen Species (RONS) affect astrocyte behaviour in physiological and pathophysiological conditions, their effects on the migration and growth of astrocytes are still unclear. Plasma-technologies are emerging in medicine as a tool to generate RONS for treating cells directly or through Plasma Activated Liquid Media (PALM). In this paper, we show for the first time how the use of PALM can modulate both astrocyte growth and migration as a function of active species produced by plasma in liquids. Our results show that PALM, generated by means of cold atmospheric pressure plasmas fed with N2, air or O2, can modulate astrocyte behaviour depending on the content of hydrogen peroxide and nitrite in the liquid. In particular, H2O2 enriched PALM induced a negative effect on cell growth associated with the mild wound healing improvement of primary astrocytes, in a scratch assay. Nitrite enriched PALM induced a selective effect on the wound healing without affecting cell growth. PALM containing a more balanced level of H2O2 and NO2− were able to affect cell growth, as well as significantly ameliorate wound healing. None of the PALM investigated induced upregulation of the gliotic inflammatory marker glial fibrillary acidic protein (GFAP), or of the astrocyte markers Aquaporin-4 (AQP4) and Connexin-43 (Cx-43) analysed by Western blot. Finally, immunofluorescence analysis revealed the presence of NO2- able to induce elongated protrusions at the front end of wounded astrocytes in the direction of cell migration. With our study we believe to have shown that PALM offer a novel tool to modulate astrocyte behaviour and that they are promising candidates for controlling astrogliosis in the case of CNS injuries.

Download Full-text