Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica

Abstract Using aqueous cyanobacterial extracts in the synthesis of silver nanoparticle is looked as green, ecofriendly, low priced biotechnology that gives advancement over both chemical and physical methods. In the current study, an aqueous extract of Oscillatoria limnetica fresh biomass was used for the green synthesis of Ag-NPs, since O. limnetica extract plays a dual part in both reducing and stabilizing Oscillatoria-silver nanoparticles (O-AgNPs). The UV-Visible absorption spectrum, Fourier transforms infrared (FT-IR), transmission electron microscopy (TEM) and scanning electron microscope (SEM) were achieved for confirming and characterizing the biosynthesized O-AgNPs. TEM images detected the quasi-spherical Ag-NPs shape with diverse size ranged within 3.30–17.97 nm. FT-IR analysis demonstrated the presence of free amino groups in addition to sulfur containing amino acid derivatives acting as stabilizing agents as well as the presence of either sulfur or phosphorus functional groups which possibly attaches silver. In this study, synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast (MCF-7) cell line giving IC50 (6.147 µg/ml) and human colon cancer (HCT-116) cell line giving IC50 (5.369 µg/ml). Hemolytic activity of Ag-NPs was investigated and confirmed as being non- toxic to human RBCs in low concentrations.

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Combined efficacy of silver nanoparticles and commercial antibiotics on different phylogenetic groups of Escherichia coli

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.21788 ◽

2019 ◽

Vol 70 (3) ◽

pp. 1647

Author(s):

A. KAZEMNIA ◽

M. AHMADI ◽

K. MARDANI ◽

M. MORADI ◽

R. DARVISHZADEH

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Ag Nps ◽

Phylogenetic Groups ◽

Profound Impact ◽

Polymeric Chains

Silver nanoparticles (Ag-NPs) can attach to flexible polymeric chains of antibiotics, hence it can be used in combination with antibiotics against resistant bacteria. In this study, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and MBC/MIC ratio of Ag-NPs and antibiotics (gentamicin, tetracycline, erythromycin, ciprofloxacin, nalidixic acid, cefixime, cephalexin, amoxicillin, ampicillin, and penicillin) were quantified against 50 Escherichia coli isolates (25 human urinary tract infection and 25 avian colibacillosis). All isolates had been assigned as four phylogenetic groups A, B1, B2, and D. The results showed that the majority of the human and broiler isolates belonged to phylogenetic groups A and B2. MBC/MIC ratio of Ag-NPs in combination with antibiotics was assessed. It was found that the MIC of the majority of broiler isolates to Ag-NPs was equal to or greater than 50 μg/ml. To conclude, a combination of penicillin and ciprofloxacin with Ag-NPs exhibited profound impact against isolates, the combinations might be applicable for treating multidrug-resistant bacteria.

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Synthesis of silk sericin stabilized silver (Ag-Ser) nanoparticles using Tollens’ method and investigation of its colloidal stability

10.21203/rs.3.rs-1141192/v1 ◽

2021 ◽

Author(s):

Mert Saraçoğlu ◽

Begüm Bacınoğlu ◽

Sıddıka Mertdinç ◽

Servet Timur

Keyword(s):

Silver Nanoparticles ◽

Colloidal Stability ◽

Fourier Transforms ◽

Production Method ◽

Face Centered Cubic ◽

Ag Nps ◽

Transmission Electron ◽

Ft Ir ◽

Amine Complex ◽

Face Centered

Abstract In this study, sericin extracted from Bombyx mori silk cocoons was integrated into the well-known Tollens’ method for synthesizing Ag-NPs. Sericin successfully acted as a stabilizer while silver amine complex [Ag(NH3)2]+ was reduced by maltose. As a result, silver nanoparticles with high stability are formed. Possible functional groups related to the stabilization of NPs were investigated by Fourier-transforms infrared spectroscopy (FT-IR). Ag-Ser NPs were characterized by using particle size measurements based on dynamic light scattering (DLS) and transmission electron microscopy (TEM). According to the characterization investigations, Ag-Ser NPs have characteristic (111) face-centered cubic (FFC) plane and were spherical in shape with a narrow size distribution of 20.23 ±6.25 nm. Overall, the sericin-modified Tollens’ method for synthesizing Ag-NPs offers a simple and non-toxic production method to form nanoparticles. Colloidal stability of nanoparticles displays an essential role since their enhanced nano-properties can be diminished by an increase in size due to aggregation and agglomeration. Therefore, the effect of pH on particle stability was investigated through the surface charge of Ag-Ser NPs that was measured using a Zeta-potential analyzer. Results obtained from this study may extend the applicability of silver nanoparticles in biotechnological researches and a potential synthesis route for the application of Ag-Ser NPs as aseptic and therapeutic usages.

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Effect of silver nanoparticles combined with antibiotics on different phylogenetic groups of Escherichia coli

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.18636 ◽

2019 ◽

Vol 70 (3) ◽

Author(s):

Ali Kazemnia

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Ag Nps ◽

Phylogenetic Groups ◽

Profound Impact ◽

Polymeric Chains

Silver nanoparticles (Ag-NPs) can attach to flexible polymeric chains of antibiotics, hence it can be used in combination with antibiotics against resistant bacteria. In this study, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and MBC/MIC ratio of Ag-NPs and antibiotics (gentamicin, tetracycline, erythromycin, ciprofloxacin, nalidixic acid, cefixime, cephalexin, amoxicillin, ampicillin, and penicillin) were quantified against 50 Escherichia coli isolates (25 human urinary tract infection and 25 avian colibacillosis). All isolates had been assigned as four phylogenetic groups A, B1, B2, and D. The results showed that the majority of the human and broiler isolates belonged to phylogenetic groups A and B2. MBC/MIC ratio of Ag-NPs in combination with antibiotics was assessed. It was found that the MIC of the majority of broiler isolates to Ag-NPs was equal to or greater than 50 µg/ml. To conclude, as combination of penicillin with Ag-NPs and ciprofloxacin with Ag-NPs exhibited profound impact against isolates, the combinations might be used against multidrug resistant bacteria.

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Biosynthesis and Characterization of Silver Nanoparticles produced by Phormidium ambiguum and Desertifilum tharense

10.21203/rs.3.rs-622253/v1 ◽

2021 ◽

Author(s):

Amira Lotfy Hanna ◽

Hayam Hamouda ◽

Hanan Goda ◽

Tarek Elsayed ◽

Mahmoud Sadik

Keyword(s):

Silver Nanoparticles ◽

Micrococcus Luteus ◽

16S Rrna Gene Sequencing ◽

Inhibition Zone ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Rrna Gene ◽

Face Centered Cubic ◽

Ag Nps ◽

Rrna Gene Sequencing

Abstract The world faces a challenge with pervasion of multidrug resistant bacteria which encouraged the scientists to develop and discover alternative ecofriendly and easy to produce new antibacterial agents. Two Egyptian cyanobacteria were isolated and identified according to 16S rRNA gene sequencing as Phormidium ambiguum and Desertifilum tharense . The sequences were deposited in the GenBank with accession numbers of MW762709 and MW762710 for Desertifilum tharense and Phormidium ambiguum, respectively. These isolates have the ability to produce silver nanoparticles (Ag-NPs) extra- and intracellularly under light and dark conditions. The results of UV-Vis analysis showed promising extracellular Ag-NPs synthesis by Desertifilum tharense and Phormidium ambiguum under light conditions. Therefore, these Ag-NPs were characterized and evaluated for antibacterial and antioxidant activity. TEM, SEM and XRD analyses revealed the spherical crystals with face-centered cubic structures and size range of 6.24–11.4 nm and 6.46–12.2 nm for Ag-NPs of Desertifilum tharense and Phormidium ambiguum , respectively. XRD and EDX results clearly confirmed the successful synthesis of Ag-NPs in its oxide form or chloride form. The FTIR spectrum data confirmed the presence of hydroxyl and amide groups. Desertifilum tharense Ag-NPs displayed the largest inhibition zone ranged from 9 mm against Micrococcus luteus ATCC 10240 to 25 mm against methicillin resistant S. aureus (MRSA) ATCC 43300. For Phormidium ambiguum Ag-NPs, the inhibition zone diameter was in a range of 9–18 mm. The Ag-NPs of Phormidium ambiguum exhibited the highest scavenging activity of 48.7% comparing with that of Desertifilum tharense which displayed 43.753%.

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Synthesis and Application of Silver Nanoparticles (Ag NPs) for the Prevention of Infection in Healthcare Workers

International Journal of Molecular Sciences ◽

10.3390/ijms20153620 ◽

2019 ◽

Vol 20 (15) ◽

pp. 3620 ◽

Cited By ~ 37

Author(s):

Shingo Nakamura ◽

Masahiro Sato ◽

Yoko Sato ◽

Naoko Ando ◽

Tomohiro Takayama ◽

...

Keyword(s):

Silver Nanoparticles ◽

Healthcare Workers ◽

High Efficiency ◽

Particle Surface ◽

Silver Ions ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Medical Field ◽

Ag Nps ◽

Environment Friendly

Silver is easily available and is known to have microbicidal effect; moreover, it does not impose any adverse effects on the human body. The microbicidal effect is mainly due to silver ions, which have a wide antibacterial spectrum. Furthermore, the development of multidrug-resistant bacteria, as in the case of antibiotics, is less likely. Silver ions bind to halide ions, such as chloride, and precipitate; therefore, when used directly, their microbicidal activity is shortened. To overcome this issue, silver nanoparticles (Ag NPs) have been recently synthesized and frequently used as microbicidal agents that release silver ions from particle surface. Depending on the specific surface area of the nanoparticles, silver ions are released with high efficiency. In addition to their bactericidal activity, small Ag NPs (<10 nm in diameter) affect viruses although the microbicidal effect of silver mass is weak. Because of their characteristics, Ag NPs are useful countermeasures against infectious diseases, which constitute a major issue in the medical field. Thus, medical tools coated with Ag NPs are being developed. This review outlines the synthesis and utilization of Ag NPs in the medical field, focusing on environment-friendly synthesis and the suppression of infections in healthcare workers (HCWs).

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Green Synthesis of Silver Nanoparticles and Evaluation of Their Antibacterial Activity against Multidrug-Resistant Bacteria and Wound Healing Efficacy Using a Murine Model

Antibiotics ◽

10.3390/antibiotics9120902 ◽

2020 ◽

Vol 9 (12) ◽

pp. 902

Author(s):

Vajravathi Lakkim ◽

Madhava C. Reddy ◽

Roja Rani Pallavali ◽

Kakarla Raghava Reddy ◽

Ch Venkata Reddy ◽

...

Keyword(s):

Electron Microscopy ◽

Wound Healing ◽

Silver Nanoparticles ◽

Multidrug Resistant ◽

Biomedical Science ◽

Resistant Bacteria ◽

Zone Of Inhibition ◽

Ag Nps ◽

Bacteriolytic Activity ◽

Vis Spectroscopy

Green nanotechnology has significant applications in various biomedical science fields. In this study, green-synthesized silver nanoparticles, prepared by using Catharanthus roseus and Azadirachta indica extracts, were characterized using UV–Vis spectroscopy, dynamic light scattering, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. Silver nanoparticles (Ag NPs) synthesized from leaf extracts of C. roseus and A. indica effectively inhibited the growth of multidrug-resistant (MDR) bacteria isolated from patients with septic wound infections. The maximum bacteriolytic activity of the green-synthesized Ag NPs of C. roseus and A. indica against the MDR bacterium K. Pneumoniae was shown by a zone of inhibition of 19 and 16 mm, respectively. C. roseus Ag NPs exhibited more bacteriolytic activity than A. indica Ag NPs in terms of the zone of inhibition. Moreover, these particles were effective in healing wounds in BALB/c mice. Ag NPs of C. roseus and A. indica enhanced wound healing by 94% ± 1% and 87% ± 1%, respectively. Our data suggest that Ag NPs from C. roseus and A. indicia ameliorate excision wounds, and wound healing could be due to their effective antimicrobial activity against MDR bacteria. Hence, these Ag NPs could be potential therapeutic agents for the treatment of wounds.

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Biogenic synthesis of gold nanoparticles using Argemone mexicana L. and their cytotoxic and genotoxic effects on human colon cancer cell line (HCT-15)

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-020-00113-y ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Kailas D. Datkhile ◽

Satish R. Patil ◽

Pratik P. Durgawale ◽

Madhavi N. Patil ◽

Dilip D. Hinge ◽

...

Keyword(s):

Cell Line ◽

Caspase 3 ◽

Cancer Cell Line ◽

Human Colon ◽

Colon Cancer Cell Line ◽

Colon Cancer Cell ◽

Human Colon Cancer Cell ◽

Genotoxic Effects ◽

Argemone Mexicana ◽

Human Colon Cancer

Abstract Background Nanomedicine has evolved as precision medicine in novel therapeutic approach of cancer management. The present study investigated the efficacy of biogenic gold nanoparticles synthesized using Argemone mexicana L. aqueous extract (AM-AuNPs) against the human colon cancer cell line, HCT-15. Results Biosynthesis of AM-AuNPs was determined by ultraviolet-visible spectroscopy and further characterized by transmission electron microscopy, X-ray diffraction, and Fourier transition infrared spectroscopy analysis. The cytotoxic activity of AM-AuNPs was assessed by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, whereas genotoxicity was evaluated by the DNA fragmentation assay. The expression of apoptosis regulatory genes such as p53 and caspase-3 was explored through semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and western blotting to evidence apoptotic cell death in HCT-15 cells. Biogenic AM-AuNPs inhibited cell proliferation in HCT-15 cell line with a half maximal inhibitory concentration (IC50) of 20.53 μg/mL at 24 h and 12.03 μg/mL at 48 h of exposure. The altered cell morphology and increased apoptosis due to AM-AuNPs were also evidenced through nuclear DNA fragmentation and upregulated expression of p53 and caspase-3 in HCT-15 cells. Conclusion The AM-AuNPs may exert antiproliferative and genotoxic effects on HCT-15 cells by cell growth suppression and induction of apoptosis mediated by activation of p53 and caspase-3 genes.

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Pharmacological Properties of Biogenically Synthesized Silver Nanoparticles using Endophyte Bacillus cereus extract of Berberis lyceum against Oxidative Stress and Pathogenic Multidrug-Resistant Bacteria

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.07.009 ◽

2021 ◽

Author(s):

Neelam Mujaddidi ◽

Sobia Nisa ◽

Samha Al Ayoubi ◽

Yamin Bibi ◽

Salman Khan ◽

...

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Bacillus Cereus ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Pharmacological Properties ◽

Multidrug Resistant Bacteria

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The Synergistic Effect of Biosynthesized Silver Nanoparticles and Phage ZCSE2 as a Novel Approach to Combat Multidrug-Resistant Salmonella enterica

Antibiotics ◽

10.3390/antibiotics10060678 ◽

2021 ◽

Vol 10 (6) ◽

pp. 678

Author(s):

Abdallah S. Abdelsattar ◽

Rana Nofal ◽

Salsabil Makky ◽

Anan Safwat ◽

Amera Taha ◽

...

Keyword(s):

Silver Nanoparticles ◽

Color Change ◽

Antibacterial Properties ◽

Multidrug Resistant ◽

Inhibitory Effect ◽

Health Concern ◽

Resistant Bacteria ◽

Mortality And Morbidity ◽

Novel Approach ◽

Transmission Electron

The emergence and evolution of antibiotic-resistant bacteria is considered a public health concern. Salmonella is one of the most common pathogens that cause high mortality and morbidity rates in humans, animals, and poultry annually. In this work, we developed a combination of silver nanoparticles (AgNPs) with bacteriophage (phage) as an antimicrobial agent to control microbial growth. The synthesized AgNPs with propolis were characterized by testing their color change from transparent to deep brown by transmission electron microscopy (TEM) and Fourier-Transform Infrared Spectroscopy (FTIR). The phage ZCSE2 was found to be stable when combined with AgNPs. Both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated for AgNPs, phage, and their combination. The results indicated that MIC and MBC values were equal to 23 µg/mL against Salmonella bacteria at a concentration of 107 CFU/mL. The combination of 0.4× MIC from AgNPs and phage with Multiplicity of Infection (MOI) 0.1 showed an inhibitory effect. This combination of AgNPs and phage offers a prospect of nanoparticles with significantly enhanced antibacterial properties and therapeutic performance.

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