scholarly journals Synthesis of silk sericin stabilized silver (Ag-Ser) nanoparticles using Tollens’ method and investigation of its colloidal stability

2021 ◽  
Author(s):  
Mert Saraçoğlu ◽  
Begüm Bacınoğlu ◽  
Sıddıka Mertdinç ◽  
Servet Timur
Keyword(s):  
Silver Nanoparticles ◽  
Colloidal Stability ◽  
Fourier Transforms ◽  
Production Method ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Transmission Electron ◽  
Ft Ir ◽  
Amine Complex ◽  
Face Centered

Abstract In this study, sericin extracted from Bombyx mori silk cocoons was integrated into the well-known Tollens’ method for synthesizing Ag-NPs. Sericin successfully acted as a stabilizer while silver amine complex [Ag(NH3)2]+ was reduced by maltose. As a result, silver nanoparticles with high stability are formed. Possible functional groups related to the stabilization of NPs were investigated by Fourier-transforms infrared spectroscopy (FT-IR). Ag-Ser NPs were characterized by using particle size measurements based on dynamic light scattering (DLS) and transmission electron microscopy (TEM). According to the characterization investigations, Ag-Ser NPs have characteristic (111) face-centered cubic (FFC) plane and were spherical in shape with a narrow size distribution of 20.23 ±6.25 nm. Overall, the sericin-modified Tollens’ method for synthesizing Ag-NPs offers a simple and non-toxic production method to form nanoparticles. Colloidal stability of nanoparticles displays an essential role since their enhanced nano-properties can be diminished by an increase in size due to aggregation and agglomeration. Therefore, the effect of pH on particle stability was investigated through the surface charge of Ag-Ser NPs that was measured using a Zeta-potential analyzer. Results obtained from this study may extend the applicability of silver nanoparticles in biotechnological researches and a potential synthesis route for the application of Ag-Ser NPs as aseptic and therapeutic usages.

scholarly journals Green synthesis of silver nanoparticles using tannins

2014 ◽  
Vol 32 (3) ◽  
pp. 408-413 ◽  
Author(s):  
Pandian Raja ◽  
Afidah Rahim ◽  
Ahmad Qureshi ◽  
Khalijah Awang
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Ions ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
Spectroscopy Analysis ◽  
Transmission Electron ◽  
Ft Ir ◽  
Face Centered

AbstractColloidal silver nanoparticles were prepared by rapid green synthesis using different tannin sources as reducing agent viz. chestnut (CN), mangrove (MG) and quebracho (QB). The aqueous silver ions when exposed to CN, MG and QB tannins were reduced which resulted in formation of silver nanoparticles. The resultant silver nanoparticles were characterized using UV-Visible, X-ray diffraction (XRD), scanning electron microscopy (SEM/EDX), and transmission electron microscopy (TEM) techniques. Furthermore, the possible mechanism of nanoparticles synthesis was also derived using FT-IR analysis. Spectroscopy analysis revealed that the synthesized nanoparticles were within 30 to 75 nm in size, while XRD results showed that nanoparticles formed were crystalline with face centered cubic geometry.

scholarly journals A Green Approach for the Synthesis of Silver Nanoparticles Using Ultrasonic Radiation’s Times in Sodium Alginate Media: Characterization and Antibacterial Evaluation

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Miftah Faried ◽  
Kamyar Shameli ◽  
Mikio Miyake ◽  
Abdollah Hajalilou ◽  
Ali Zamanian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Sodium Alginate ◽  
Spherical Shape ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Green Approach ◽  
Resonance Band ◽  
Ft Ir ◽  
Face Centered

The synthesis of silver nanoparticles (Ag-NPs) was achieved by a simple green chemistry procedure using sodium alginate (Na-Alg) under ultrasonic radiation as a stabilizer and physical reducing agent. The effect of radiation time on the synthesis of Ag-NPs was carried out at room temperature until 720 min. The successful formation of Ag-NPs has been confirmed by UV-Vis, XRD, TEM, FESEM-EDX, zeta potential, and FT-IR analyses. The surface plasmon resonance band appeared at the range of 452–465 nm that is an evidence of formation of Ag-NPs. The XRD study showed that the particles are crystalline structure in nature, with a face-centered cubic (fcc) structure. The TEM study showed the Ag-NPs have average diameters of around 20.16–22.38 nm with spherical shape. The FESEM-EDX analysis confirmed the spherical shape of Ag-NPs on the surface of Alg and the element of Ag with the high purity. The zeta potential showed high stability of Alg/Ag-NPs especially after 720 min irradiation with value of −67.56 mV. The FT-IR spectrum confirmed that the Ag-NPs have been capped by the Alg with van der Waals interaction. The Alg/Ag-NPs showed the antibacterial activity against Gram-positive and Gram-negative bacteria. These suggest that Ag-NPs can be employed as an effective bacteria inhibitor and can be applied in medical field.

scholarly journals Green Synthesis of Silver Nanoparticles using Salacca zalacca Extract as Reducing Agent and It’s Antibacterial Activity

2019 ◽  
Vol 31 (12) ◽  
pp. 2804-2810
Author(s):  
Anti Kolonial Prodjosantoso ◽  
Oktanio Sigit Prawoko ◽  
Maximus Pranjoto Utomo ◽  
Lis Permana Sari
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Silver Ions ◽  
Reduction Reaction ◽  
Face Centered Cubic ◽  
Gram Positive Bacteria ◽  
Transmission Electron ◽  
Face Centered

In this article, the synthesis of silver nanoparticles through a reduction reaction process using Salacca zalacca extract is reported. The AgNPs were characterized using X-ray diffraction, transmission electron microscopy, Fourier transform infrared and UV-visible spectrophotometry methods. The AgNPs antibacterial activity was determined against of Gram-positive bacteria (Staphylococcus epidermidis) and Gram-negative bacteria (Escherichia coli). The main functional groups contained in Salacca zalacca extract are carbonyl, hydroxyl and nitrile groups, which are believed to reduce the silver ions to metal. The surface plasmon resonance values of brownish red AgNPs are in the range of 410 nm to 460 nm. The structure of AgNPs is face centered cubic (FCC). The diameter of silver nanoparticles crystallite is 14.2 ± 2.6 nm. The AgNPs growth inhibition zones of Escherichia coli and Staphylococcus epidermidis are 9.6 mm and 9.2 mm, respectively.

scholarly journals Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ragaa A. Hamouda ◽  
Mervat H. Hussein ◽  
Rasha A. Abo-elmagd ◽  
Salwa S. Bawazir
Keyword(s):  
Silver Nanoparticles ◽  
Cell Line ◽  
Fourier Transforms ◽  
Human Colon ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Ag Nps ◽  
Human Colon Cancer ◽  
Visible Absorption ◽  
Ft Ir

Abstract Using aqueous cyanobacterial extracts in the synthesis of silver nanoparticle is looked as green, ecofriendly, low priced biotechnology that gives advancement over both chemical and physical methods. In the current study, an aqueous extract of Oscillatoria limnetica fresh biomass was used for the green synthesis of Ag-NPs, since O. limnetica extract plays a dual part in both reducing and stabilizing Oscillatoria-silver nanoparticles (O-AgNPs). The UV-Visible absorption spectrum, Fourier transforms infrared (FT-IR), transmission electron microscopy (TEM) and scanning electron microscope (SEM) were achieved for confirming and characterizing the biosynthesized O-AgNPs. TEM images detected the quasi-spherical Ag-NPs shape with diverse size ranged within 3.30–17.97 nm. FT-IR analysis demonstrated the presence of free amino groups in addition to sulfur containing amino acid derivatives acting as stabilizing agents as well as the presence of either sulfur or phosphorus functional groups which possibly attaches silver. In this study, synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast (MCF-7) cell line giving IC50 (6.147 µg/ml) and human colon cancer (HCT-116) cell line giving IC50 (5.369 µg/ml). Hemolytic activity of Ag-NPs was investigated and confirmed as being non- toxic to human RBCs in low concentrations.

scholarly journals Biosynthesis of Silver Nanoparticles Using Ganoderma Lucidum and Assessment of Antioxidant and Antibacterial Activity

2017 ◽  
Vol 5 (4) ◽  
pp. 523-531 ◽  
Author(s):  
Milan Poudel ◽  
Rabin Pokharel ◽  
Sudip K.C. ◽  
Suvash Chandra Awal ◽  
Rajaram Pradhananga
Keyword(s):  
Silver Nanoparticles ◽  
Ganoderma Lucidum ◽  
Fold Increase ◽  
Diffusion Method ◽  
Maximum Effect ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Antimicrobial Studies ◽  
Green Route ◽  
Face Centered

There is an increasing interest these days in the green route of synthesis of metal nanoparticles using plant extract, fungus and bacterial- mediated eco-friendly materials. Silver nanoparticles were synthesized using an intracellular extract of Ganoderma lucidum, a mushroom from Nepal then characterization of silver nanoparticles was performed. The Ag-NPs thus formed show surface plasmonic resonance with a maximum absorption band at 420 nm. Their crystalline nature was confirmed as a face-centered cubic structure by the XRD, Furthermore, SEM revealed that they were in the size range of 10-30 nm and were spherical in shape. The possible biomolecule involved in the reduction and stabilization of Ag-NPs were believed to be oxidized polyphenol, and carbonyl group, amino acid residue. Ag-NPs exhibit good antioxidant activity but showed low antioxidant in comparison to the fungal extract alone, which was studied using DPPH antioxidant assay. The effect of the colloidal silver nanoparticles solution against six human pathological bacteria was carried out by Disc diffusion method. The zone of maximum inhibition was seen in Bacillus subtilis (17.0 ± 0.13 mm) and least effective against Escherichia coli (10.1 ± 0.2 mm). Further, the results showed that Ag-NPs in combination with antibiotics have better antibacterial effect as compared with Ag-NPs alone. The maximum effect with a 3.2 and 5.3 fold increase was seen in Gentamicin and Streptomycin respectively providing the synergistic role of Ag-NPs. The results of antimicrobial studies indicated that the Ag-NPs are the metal of choice and can be effectively used in combination with antibiotics in order to improve their efficiency against various pathogenic microbes. Int. J. Appl. Sci. Biotechnol. Vol 5(4): 523-531

scholarly journals Biosynthesis of silver nanoparticles using Pomegranate juice extract and its antibacterial activity

2016 ◽  
Vol 4 (3) ◽  
pp. 254-258 ◽  
Author(s):  
Mona Hussein Ibraheim ◽  
A.A. Ibrahiem ◽  
T. R. Dalloul
Keyword(s):  
Silver Nanoparticles ◽  
Fourier Transforms ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Pomegranate Juice ◽  
Face Centered Cubic ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Biosynthesized Agnps ◽  
High Level

Green synthesis of silver nanoparticles (AgNPs) from silver nitrate was carried out using aqueous Pomegranate juice extract (PJE) as a reducing agent. The formation of AgNPs was characterized by UV-visible (UV–vis) spectroscopy, transmission electron microscopy (TEM), Fourier transforms infrared spectroscopy and X-ray diffraction (XRD). Surface Plasmon resonance (SPR) of ∼420-423 nm confirmed the earlier formation of AgNPs. TEM and XRD analysis showed that the AgNPs with an average diameter of 23 nm are crystalline in nature and have face-centered cubic geometry. The antibacterial efficiency of AgNPs against Escherchia coli and Staphylococcus aureus showed high level of inhibition. Further, the zone of inhibition increased with the increase in the concentration of silver nanoparticles. These studies are quite useful as it shows the utility of green nanotechnology for the synthesis of silver nanoparticles without any toxic residuals and byproducts. The efficient antimicrobial activity of biosynthesized AgNPs proves the application potential in the area of nano-medicine.Int J Appl Sci Biotechnol, Vol 4(3): 254-258

scholarly journals Green Synthesis of Silver Nanoparticles Using Bilberry and Red Currant Waste Extracts

Processes ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 193 ◽  
Author(s):  
Antonio Zuorro ◽  
Annalaura Iannone ◽  
Stefano Natali ◽  
Roberto Lavecchia
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Hydrodynamic Diameter ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Stabilizing Agents ◽  
Resonance Band ◽  
Effects Of Temperature ◽  
Face Centered ◽  
Red Currant

The production of silver nanoparticles (Ag-NPs) from bilberry waste (BW) and red currant waste (RCW) extracts was studied. Red fruit extracts were obtained by treating BW and RCW with aqueous ethanol (50% v/v) at 40 °C. The formation of nanoparticles was monitored spectrophotometrically by measuring the intensity of the surface plasmon resonance band (SPR) of silver. The effects of temperature (20–60 °C) and pH (8–12) on the reaction kinetics and on the properties of Ag-NPs were investigated. Characterization by XRD and dynamic light scattering (DLS) techniques showed that Ag-NPs were highly crystalline, with a face-centered cubic structure and a hydrodynamic diameter of 25–65 nm. The zeta potential was in the range of −35.6 to −20.5 mV. Nanoparticles obtained from BW were slightly smaller and more stable than those from RCW. A kinetic analysis by the initial-rate method showed that there was an optimum pH, around 11, for the production of Ag-NPs. Overall, the results obtained suggest that BW and RCW can be advantageously used as a source of reducing and stabilizing agents for the green synthesis of Ag-NPs.

High photoelectric PPV/PVA/Ag composite nanofibers by co-electrospinning

2015 ◽  
Vol 35 (7) ◽  
pp. 689-697 ◽  
Author(s):  
Yang Gao ◽  
Zhiyao Sun ◽  
Liguo Sun ◽  
Cheng Wang ◽  
Shuhong Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Composite Nanofibers ◽  
Average Diameter ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
Ag Nps ◽  
Photoelectric Properties ◽  
Transmission Electron ◽  
Face Centered ◽  
Xrd Patterns

Abstract Poly(phenylene vinylene)/polyvinyl alcohol/Ag (PPV/PVA/Ag) composite nanofibers with excellent photoelectric properties were prepared by coaxial electrospinning using PPV/PVA as the shell and Ag nanoparticles (NPs) as the core, Ag NPs aqueous solution was prepared by the reduction method. The results of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that Ag NPs are of a face-centered cubic structure, with an average diameter of 46 nm and the composite nanofibers have uniform and continuous morphology. With increasing Ag content, the diameters of the composite nanofibers decreased from 653 nm to 250 nm. The X-ray diffraction (XRD) patterns verified that in the composite nanofibers, the Ag NPs are not transformed. In the photoluminescence spectra, the PPV/PVA/Ag composite nanofibers presented red-shift compared with PPV/PVA nanofibers. Under illumination, the as-prepared PPV/PVA/Ag composite nanofibers exhibited relatively high photocurrent intensity.

Green Synthetic Nanoarchitectonics of Gold and Silver Nanoparticles Prepared Using Quercetin and Their Cytotoxicity and Catalytic Applications

2020 ◽  
Vol 20 (5) ◽  
pp. 2781-2790 ◽  
Author(s):  
You Jeong Lee ◽  
Youmie Park
Keyword(s):  
Silver Nanoparticles ◽  
Anticancer Agents ◽  
Biologically Active ◽  
Face Centered Cubic ◽  
Gold And Silver Nanoparticles ◽  
Transmission Electron ◽  
Nitrophenol Reduction ◽  
Face Centered ◽  
Cytotoxicity Assessment ◽  
Average Size

Quercetin is a flavonoid and is abundant in the plant kingdom. Green nanoparticles (gold and silver) were synthesized by using quercetin as a reductant via a green route for their potential nanoarchitechtonic applications. There were no toxic chemicals involved during the synthesis. The gold and silver nanoparticles exhibited surface plasmon resonance at 527 nm and 401 nm, respectively. Both nanoparticle solutions retained excellent colloidal shelf stability for 7 days and in cell culture medium. The crystal structure of the nanoparticles was observed by X-ray diffraction analysis. Field emission transmission electron microscopy images revealed that spherical nanoparticles were synthesized, with an average size of 20.2±4.8 nm for gold nanoparticles and 32.4±14.0 nm for silver nanoparticles. Observation of clear lattice fringes in the microscopic images suggested that both types of nanoparticles possessed a face-centered cubic structure. Catalytic activity was evaluated with respect to 4-nitrophenol reduction and methyl orange degradation. When increasing the amount of gold or silver nanoparticles used as a catalyst, the rate constant of the catalytic reaction was also increased. Cytotoxicity assessment on cancer cells demonstrated that both types of nanoparticles can be appropriate candidates for delivery vehicles of biologically active molecules, such as anticancer agents.

Faceted antiphase boundaries in GaAs grown on Ge

1987 ◽  
Vol 45 ◽  
pp. 314-315
Author(s):  
N.-H. Cho ◽  
S. McKernan ◽  
C.B. Carter ◽  
K. Wagner
Keyword(s):  
Cross Section ◽  
Atomic Resolution ◽  
Face Centered Cubic ◽  
Electrical Behavior ◽  
Sphalerite Structure ◽  
Antiphase Boundaries ◽  
Transmission Electron ◽  
Face Centered ◽  
Quality Material ◽  
Simulated Images

Interest has recently increased in the possibility of growing III-V compounds epitactically on non-polar substrates to produce device quality material. Antiphase boundaries (APBs) may then develop in the GaAs epilayer because it has sphalerite structure (face-centered cubic with a two-atom basis). This planar defect may then influence the electrical behavior of the GaAs epilayer. The orientation of APBs and their propagation into GaAs epilayers have been investigated experimentally using both flat-on and cross-section transmission electron microscope techniques. APBs parallel to (110) plane have been viewed at the atomic resolution and compared to simulated images.Antiphase boundaries were observed in GaAs epilayers grown on (001) Ge substrates. In the image shown in Fig.1, which was obtained from a flat-on sample, the (110) APB planes can be seen end-on; the faceted APB is visible because of the stacking fault-like fringes arising from a lattice translation at this interface.

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