scholarly journals Effect of shell structure of Ti-immobilized metal ion affinity chromatography core-shell magnetic particles for phosphopeptide enrichment

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Anna Laura Capriotti ◽  
Michela Antonelli ◽  
Diego Antonioli ◽  
Chiara Cavaliere ◽  
Riccardo Chiarcos ◽  
...  
Keyword(s):  
Metal Ion ◽  
High Performance ◽  
Magnetic Particles ◽  
Iminodiacetic Acid ◽  
Surface Characteristics ◽  
Magnetic Core ◽  
Material Surface ◽  
Water Contact ◽  
Grand Average ◽  
Phosphorylated Peptides

Abstract Magnetic materials in sample preparation for shotgun phosphoproteomics offer several advantages over conventional systems, as the enrichment can be achieved directly in solution, but they still suffer from some drawbacks, due to limited stability and selectivity, which is supposed to be affected by the hydrophilicity of the polymeric supports used for cation immobilization. The paper describes the development of an improved magnetic material with increased stability, thanks to a two-step covering of the magnetic core, for the enrichment of phosphopeptides in biological samples. Four materials were prepared featuring a polymeric shell with tunable hydrophilicity, obtained by “grafting from” polymerization of glycidyl methacrylate with 0–8.3% of polyethylene glycol methacrylate (PEGMA), the latter used to modulate the hydrophilicity of the material surface. Finally, the materials were functionalized with iminodiacetic acid for Ti4+ ion immobilization. The materials were analyzed for their composition by a combination of CHN elemental analysis and thermogravimetric analysis, also hyphenated to gas chromatography and mass spectrometric detection. Surface characteristics were evaluated by water contact angle measurements, scanning electron microscopy and energy dispersive X-ray spectrometry. These materials were applied to the enrichment of phosphopeptides from yeast protein digests. Peptides were identified by proteomics techniques using nano-high performance liquid chromatography coupled to mass spectrometry and bioinformatics. Qualitatively the peptides identified by the four systems were comparable, with 1606–1693 phosphopeptide identifications and a selectivity of 47–54% for all materials. The physico-chemical features of the identified peptides were also the same for the four materials. In particular, the grand average of hydropathy index values indicated that the enriched phosphopeptides were hydrophilic (ca. 90%), and only some co-enriched non-phosphorylated peptides were hydrophobic (21–28%), regardless of the material used for enrichment. Peptides had a pI ≤ 7, which indicated a well-known bias for acidic peptides binding, attributed to the interaction with the metal center itself. The results indicated that the enrichment of phosphopeptides and the co-enrichment of non-phosphorylated peptides is mainly driven by interactions with Ti4+ and does not depend on the amount of PEGMA chains in the polymer shell.

Adsorption of endotoxins on Ca2+ iminodiacetic acid by metal ion affinity chromatography

2013 ◽  
Vol 30 (11) ◽  
pp. 1194-1202
Author(s):  
LOPES André Moreni ◽  
ROMEU Jorge Sánchez ◽  
MEIRELES Rolando Páez ◽  
PERERA Gabriel Marquez ◽  
MORALES Rolando Perdomo ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Metal Ion ◽  
Iminodiacetic Acid ◽  
Metal Ion Affinity Chromatography

scholarly journals Ag-Functionalized Si Nanowire Arrays Aligned Vertically for SERS Detection of Captured Heavy Metal Ions by BSA

Coatings ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 685
Author(s):  
Ai-Huei Chiou ◽  
Jun-Luo Wei ◽  
Ssu-Han Chen
Keyword(s):  
Heavy Metal ◽  
Raman Scattering ◽  
Metal Ions ◽  
Metal Ion ◽  
Heavy Metal Ions ◽  
Great Promise ◽  
Ag Nps ◽  
Water Contact ◽  
Oh Groups ◽  
Si Nanowire

A novel surface-enhanced Raman scattering (SERS)-based probe to capture heavy metal ion (Zn2+) by bovine serum albumin (BSA) using Si-nanowire (SiNW) arrays with silver nanoparticles (AgNPs) was developed. A layer with AgNPs was deposited on the SiNW surface by RF magnetron sputtering for enhancement of SERS signals. Using a high-resolution transmission electron microscope (HRTEM), the observation reveals that the AgNP layer with depths of 30–75 nm was successfully deposited on SiNW arrays. The Ag peaks in EDS and XRD spectra of SiNW arrays confirmed the presence of Ag particles on SiNW arrays. The WCA observations showed a high affinity of the Ag–SiNW arrays immobilized with BSA (water contact angle (WCA) = 87.1°) and ZnSO4 (WCA = 8.8°). The results of FTIR analysis illustrate that the conjugate bonds exist between zinc sulfate (ZnSO4) and –OH groups/–NH groups of BSA. The resulting SiNWs/Ag NPs composite interfaces showed large Raman scattering enhancement for the capture of heavy metal ions by BSA with a detection of 0.1 μM. BSA and ZnSO4 conjugations, illustrating specific SERS spectra with high sensitivity, which suggests great promise in developing label-free biosensors.

scholarly journals Titanium oral implants: surface characteristics, interface biology and clinical outcome

2010 ◽  
Vol 7 (suppl_5) ◽  
Author(s):  
Anders Palmquist ◽  
Omar M. Omar ◽  
Marco Esposito ◽  
Jukka Lausmaa ◽  
Peter Thomsen
Keyword(s):  
Surface Properties ◽  
Cellular Response ◽  
Randomized Clinical Trials ◽  
Three Dimensional ◽  
Cell Communication ◽  
Surface Characteristics ◽  
Titanium Implants ◽  
Material Surface ◽  
Oral Implants

Bone-anchored titanium implants have revolutionized oral healthcare. Surface properties of oral titanium implants play decisive roles for molecular interactions, cellular response and bone regeneration. Nevertheless, the role of specific surface properties, such as chemical and phase composition and nanoscale features, for the biological in vivo performance remains to be established. Partly, this is due to limited transfer of state-of-the-art preparation techniques to complex three-dimensional geometries, analytical tools and access to minute, intact interfacial layers. As judged by the available results of a few randomized clinical trials, there is no evidence that any particular type of oral implant has superior long-term success. Important insights into the recruitment of mesenchymal stem cells, cell–cell communication at the interface and high-resolution imaging of the interface between the surface oxide and the biological host are prerequisites for the understanding of the mechanisms of osseointegration. Strategies for development of the next generation of material surface modifications for compromised tissue are likely to include time and functionally programmed properties, pharmacological modulation and incorporation of cellular components.

Recent Advances of Metal Phosphates-based Electrodes for High-Performance Metal Ion Batteries

2021 ◽  
Author(s):  
Qihui Cheng ◽  
Xun Zhao ◽  
Guiyuan Yang ◽  
Lei Mao ◽  
Fangfang Liao ◽  
...  
Keyword(s):  
Metal Ion ◽  
High Performance ◽  
Metal Phosphates ◽  
Recent Advances

Ultrahigh energy storage and ultrafast ion diffusion in borophene-based anodes for rechargeable metal ion batteries

2017 ◽  
Vol 5 (5) ◽  
pp. 2328-2338 ◽  
Author(s):  
Dewei Rao ◽  
Lingyan Zhang ◽  
Zhaoshun Meng ◽  
Xirui Zhang ◽  
Yunhui Wang ◽  
...  
Keyword(s):  
Energy Storage ◽  
Metal Ion ◽  
High Performance ◽  
Storage Systems ◽  
Ultrahigh Energy ◽  
Ion Diffusion ◽  
Energy Storage Systems ◽  
Increasing Demand

Since the turn of the new century, the increasing demand for high-performance energy storage systems has generated considerable interest in rechargeable ion batteries.

Solution to Inverse Problem of Manufacturing by Surface Modification With Controllable Surface Integrity Correlated to Performance: A Case Study of Thermally Sprayed Coatings for Wear Performance

2017 ◽  
Vol 139 (6) ◽  
Author(s):  
X. P. Zhu ◽  
P. C. Du ◽  
Y. Meng ◽  
M. K. Lei ◽  
D. M. Guo
Keyword(s):  
Inverse Problem ◽  
Surface Modification ◽  
Surface Integrity ◽  
High Performance ◽  
Surface Characteristics ◽  
Spray Angle ◽  
Functional Surface ◽  
Machining Processes ◽  
Wear Performance ◽  
Surface Features

Inverse problem of manufacturing is studied under a framework of high performance manufacturing of components with functional surface layer, where controllable generation of surface integrity is emphasized due to its pivotal role determining final performance. Surface modification techniques capable of controlling surface integrity are utilized to verify such a framework of manufacturing, by which the surface integrity desired for a high performance can be more effectively achieved as reducing the material and geometry constraints of manufacturing otherwise unobtainable during conventional machining processes. Here, thermal spraying of WC–Ni coatings is employed to coat stainless steel components for water-lubricated wear applications, on which a strategy for direct problem from process to performance is implemented with surface integrity adjustable through spray angle and inert N2 shielding. Subsequently, multiple surface integrity parameters can be evaluated to identify the major ones responsible for wear performance by elucidating the wear mechanism, involving surface features (coating porosity and WC phase retention) and surface characteristics (microhardness, elastic modulus, and toughness). The surface features predominantly determine tribological behaviors of coatings in combination with the surface characteristics that are intrinsically associated with the surface features. Consequently, the spray process with improved N2 shielding is designed according to the desired surface integrity parameters for higher wear resistance. It is demonstrated that the correlations from processes to performance could be fully understood and established via controllable surface integrity, facilitating solution to inverse problem of manufacturing, i.e., realization of a material and geometry integrated manufacturing.

scholarly journals Nanocelllulose Based Adsorbents for Heavy Metal Ions Removal

2021 ◽  
Author(s):  
Rongrong Si ◽  
Daiqi Wang ◽  
Yehong Chen ◽  
Dongmei Yu ◽  
Qijun Ding ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Ions ◽  
Metal Ion ◽  
Heavy Metal Ions ◽  
High Performance ◽  
Advanced Technologies ◽  
Good Biocompatibility ◽  
Heavy Metal Ions Removal ◽  
Metal Ions Removal

Abstract Heavy metal ion pollutions are of serious threat for our human health, and advanced technologies on removal of heavy metal ions in water or soil are in the focus of intensive research worldwide. Nanocellulose based adsorbents are emerging as an environmentally friendly appealing materials platform for heavy metal ions removal as nanocellulose has higher specific surface area, excellent mechanical properties and good biocompatibility. In this review, we briefly compare the differences of three kinds of nanocellulose and their preparation method. Then we cover the most recent work on nanocellulose based adsorbents for heavy metal ions removal, and present an in-depth discussion of the modification technologies for nanocellulose in assembling high performance heavy ions adsorbent process. By introducing functional groups, such as amino, carboxyl, phenolic hydroxyl, and thiol, the nanocellulose based adsorbents not only remove single heavy metal ions through ion exchange, chelation/complexation/coordination, electrostatic attraction, hydrophobic actions, binding affinity and redox reactions, but also can selectively adsorb multiple heavy ions in water. Finally, some challenges of nanocellulose based adsorbents for heavy metal ions are also prospected. We anticipate that the review supplies some guides for nanocellulose based adsorbents applied in heavy metal ions removal field.

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