scholarly journals Anatomical study of the coffee berry borer (Hypothenemus hampei) using micro-computed tomography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ignacio Alba-Alejandre ◽  
Javier Alba-Tercedor ◽  
Fernando E. Vega
Keyword(s):  
Computed Tomography ◽  
Future Research ◽  
Micro Ct ◽  
Ct Reconstruction ◽  
Hypothenemus Hampei ◽  
Internal Anatomy ◽  
Micro Computed Tomography ◽  
Coffee Berry ◽  
Coffee Berry Borer ◽  
Teaching Aids

AbstractTraditionally, the study of anatomy in insects has been based on dissection techniques. Micro-computed tomography (micro-CT) is an X-ray based technique that allows visualization of the internal anatomy of insects in situ and does not require dissections. We report on the use of micro-CT scans to study, in detail, the internal structures and organs of the coffee berry borer (Hypothenemus hampei), the most damaging insect pest of coffee worldwide. Detailed images and videos allowed us to make the first description of the aedeagus and the first report of differences between the sexes based on internal anatomy (flight musculature, midgut shape, hindgut convolutions, brain shape and size) and external morphology (lateral outline of the pronotum and number of abdominal tergites). This study is the first complete micro-CT reconstruction of the anatomy of an insect and is also the smallest insect to have been evaluated in this way. High quality rendered images, and additional supplementary videos and 3D models are suitable for use with mobile devices and are useful tools for future research and as teaching aids.

scholarly journals Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Javier Alba-Tercedor ◽  
Wayne B. Hunter ◽  
Ignacio Alba-Alejandre
Keyword(s):  
Computed Tomography ◽  
Significant Advance ◽  
Micro Ct ◽  
Diaphorina Citri ◽  
Ct Reconstruction ◽  
Internal Anatomy ◽  
Micro Computed Tomography ◽  
Citrus Leaf ◽  
Teaching Aids ◽  
Adult Feeding

AbstractThe Asian citrus psyllid (ACP), Diaphorina citri, is a harmful pest of citrus trees that transmits Candidatus Liberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants. Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction of the anatomy of a psylloid insect and includes a 3D reconstruction of an adult feeding on a citrus leaf that can be used on mobile devices. Detailed rendered images and videos support first descriptions of coxal and scapus antennal glands and sexual differences in the internal anatomy (hindgut rectum, mesothoracic ganglion and brain). This represents a significant advance in our knowledge of ACP anatomy, and of psyllids in general. Together the images, videos and 3D model constitute a unique anatomical atlas and are useful tools for future research and as teaching aids.

scholarly journals Revealing the respiratory system of the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae: Scolytinae) using micro-computed tomography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Javier Alba-Tercedor ◽  
Ignacio Alba-Alejandre ◽  
Fernando E. Vega
Keyword(s):  
Computed Tomography ◽  
Respiratory System ◽  
Insect Pest ◽  
Supplementary Information ◽  
Future Research ◽  
Hypothenemus Hampei ◽  
Micro Computed Tomography ◽  
Coffee Berry ◽  
Coffee Berry Borer ◽  
The Public

AbstractThe coffee berry borer (Hypothenemus hampei) is the most economically important insect pest of coffee globally. Micro-computed tomography (micro-CT) was used to reconstruct the respiratory system of this species for the first time; this is the smallest insect (ca. 2 mm long) for which this has been done to date. Anatomical details of the spiracles and tracheal tubes are described, images presented, and new terms introduced. The total volume and the relationship between tracheal lumen diameter, length and volume are also presented. The total length of the tracheal tubes are seventy times the length of the entire animal. Videos and a 3D model for use with mobile devices are included as supplementary information; these could be useful for future research and for teaching insect anatomy to students and the public in general.

scholarly journals Publisher Correction: Anatomical study of the coffee berry borer (Hypothenemus hampei) using micro-computed tomography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ignacio Alba-Alejandre ◽  
Javier Alba-Tercedor ◽  
Fernando E. Vega
Keyword(s):  
Computed Tomography ◽  
Anatomical Study ◽  
Hypothenemus Hampei ◽  
Micro Computed Tomography ◽  
Coffee Berry ◽  
Coffee Berry Borer

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Publisher Correction: Anatomical study of the coffee berry borer (Hypothenemus hampei) using micro-computed tomography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ignacio Alba-Alejandre ◽  
Javier Alba-Tercedor ◽  
Fernando E. Vega
Keyword(s):  
Computed Tomography ◽  
Anatomical Study ◽  
Hypothenemus Hampei ◽  
Micro Computed Tomography ◽  
Coffee Berry ◽  
Coffee Berry Borer

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Coffee Berry Borer (Hypothenemus hampei), a Global Pest of Coffee: Perspectives from Historical and Recent Invasions, and Future Priorities

Insects ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 882
Author(s):  
Melissa A. Johnson ◽  
Claudia Patricia Ruiz-Diaz ◽  
Nicholas C. Manoukis ◽  
Jose Carlos Verle Rodrigues
Keyword(s):  
Insect Pest ◽  
Management Strategies ◽  
Invasion Biology ◽  
Cost Effective ◽  
Future Research ◽  
Successful Implementation ◽  
Hypothenemus Hampei ◽  
Coffee Berry ◽  
Management Options ◽  
Coffee Berry Borer

Coffee berry borer (Hypothenemus hampei (Ferrari), CBB) has invaded nearly every coffee-producing country in the world, and it is commonly recognized as the most damaging insect pest of coffee. While research has been conducted on this pest in individual coffee-growing regions, new insights may be gained by comparing and contrasting patterns of invasion and response across its global distribution. In this review, we explore the existing literature and focus on common themes in the invasion biology of CBB by examining (1) how it was introduced into each particular region and the response to its invasion, (2) flight activity and infestation patterns, (3) economic impacts, and (4) management strategies. We highlight research conducted over the last ten years in Hawaii as a case study for the development and implementation of an effective integrated pest management (IPM) program for CBB, and also discuss biosecurity issues contributing to incursion and establishment. Potential areas for future research in each of the five major components of CBB IPM (monitoring and sampling, cultural, biological, chemical, and physical controls) are also presented. Finally, we emphasize that outreach efforts are crucial to the successful implementation of CBB IPM programs. Future research programs should strive to include coffee growers as much as possible to ensure that management options are feasible and cost-effective.

scholarly journals Complex Odontoma: A Case Report with Micro-Computed Tomography Findings

2016 ◽  
Vol 2016 ◽  
pp. 1-6
Author(s):  
L. A. N. Santos ◽  
L. J. Lopes ◽  
G. D. Roque-Torres ◽  
V. F. Oliveira ◽  
D. Q. Freitas
Keyword(s):  
Computed Tomography ◽  
Third Molar ◽  
Radiographic Examination ◽  
Future Research ◽  
Benign Tumors ◽  
Bone Lesions ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Complex Odontoma ◽  
Histopathologic Evaluation

Odontomas are the most common benign tumors of odontogenic origin. They are normally diagnosed on routine radiographs, due to the absence of symptoms. Histopathologic evaluation confirms the diagnosis especially in cases of complex odontoma, which may be confused during radiographic examination with an osteoma or other highly calcified bone lesions. The micro-CT is a new technology that enables three-dimensional analysis with better spatial resolution compared with cone beam computed tomography. Another great advantage of this technology is that the sample does not need special preparation or destruction in the sectioned area as in histopathologic evaluation. An odontoma with CBCT and microtomography images is presented in a 26-year-old man. It was first observed on panoramic radiographs and then by CBCT. The lesion and the impacted third molar were surgically excised using a modified Neumann approach. After removal, it was evaluated by histopathology and microtomography to confirm the diagnostic hypothesis. According to the results, micro-CT enabled the assessment of the sample similar to histopathology, without destruction of the sample. With further development, micro-CT could be a powerful diagnostic tool in future research.

scholarly journals Evaluation of Root Canal Filling with a Bioceramic Sealer Using Micro-Computed Tomography: A Pilot Study

2019 ◽  
Vol 890 ◽  
pp. 275-282
Author(s):  
Isabel Vasconcelos ◽  
Mário Rito Pereira ◽  
António Ginjeira ◽  
Margarida C. Franco ◽  
Pedro Morouço ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Pilot Study ◽  
Root Canal ◽  
Future Research ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Root Canal Filling ◽  
Gutta Percha ◽  
Single Cone ◽  
Ah Plus

This pilot study aims to define a protocol for optimizing the micro-computed tomography (micro-CT) settings to evaluate in the future research the root canal filling in oval shaped canals. Thirty distal canals of mandibular molars were used. After preparation of the canals, the roots were randomly divided into five groups. The canals were filled with two types of sealers (Endosequence BC Sealer or AH Plus) and two types of gutta percha (Protaper Next cones and Endosequence BC cones), using thermal obturation in comparison with a single cone obturation technique. Each specimen was scanned three times using a micro-CT device at a resolution of 30,1 μm. The first scan was done for selecting the specimen according the inclusion criteria, the second one post-instrumentation and the last micro-CT scan after obturation the specimens. For the present study, the images were only evaluated with qualitative criteria and the settings for acquisition, reconstruction and analysis of micro-CT images were tested.

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