Transcriptomic analysis of the mechanisms of alleviating renal interstitial fibrosis using the traditional Chinese medicine Kangxianling in a rat model

Renal interstitial fibrosis (RIF) is the main pathological manifestation of end-stage renal disease. Recent studies have shown that endoplasmic reticulum (ER) stress is involved in the pathogenesis and development of RIF. Traditional Chinese medicine (TCM), as an effective treatment for kidney diseases, can improve kidney damage by affecting the apoptotic signaling pathway mediated by ER stress. This article reviews the apoptotic pathways mediated by ER stress, including the three major signaling pathways of unfolded protein response, the main functions of the transcription factor C/EBP homologous protein. We also present current research on TCM treatment of RIF, focusing on medicines that regulate ER stress. A new understanding of using TCM to treat kidney disease by regulating ER stress will promote clinical application of Chinese medicine and discovery of new drugs for the treatment of RIF.

Download Full-text

The study on the material basis and the mechanism for anti-renal interstitial fibrosis efficacy of rhubarb through integration of metabonomics and network pharmacology

Molecular BioSystems ◽

10.1039/c4mb00573b ◽

2015 ◽

Vol 11 (4) ◽

pp. 1067-1078 ◽

Cited By ~ 28

Author(s):

Zheng Xiang ◽

Hao Sun ◽

Xiaojun Cai ◽

Dahui Chen ◽

Xiaoyong Zheng

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Mechanism Of Action ◽

Interstitial Fibrosis ◽

Network Pharmacology ◽

Renal Interstitial Fibrosis ◽

Material Basis

The cooperative material basis of the multi-component and multi-target mechanism of action of Traditional Chinese Medicine (TCM) is difficult to elucidate because of the current lack of appropriate techniques and strategies.

Download Full-text

Silencing of SOCS‐1 and SOCS‐3 suppresses renal interstitial fibrosis by alleviating renal tubular damage in a rat model of hydronephrosis

Journal of Cellular Biochemistry ◽

10.1002/jcb.26382 ◽

2017 ◽

Vol 119 (2) ◽

pp. 2200-2211 ◽

Cited By ~ 5

Author(s):

Gui‐Hong Zheng ◽

Yong‐Jian Wang ◽

Xin Wen ◽

Xin‐Rui Han ◽

Min Shen ◽

...

Keyword(s):

Rat Model ◽

Interstitial Fibrosis ◽

Tubular Damage ◽

Renal Interstitial Fibrosis ◽

Renal Tubular Damage ◽

Renal Tubular

Download Full-text

Dihydromyricetin promotes autophagy and attenuates renal interstitial fibrosis by regulating miR-155-5p/PTEN signaling in diabetic nephropathy

Bosnian Journal of Basic Medical Sciences ◽

10.17305/bjbms.2019.4410 ◽

2019 ◽

Author(s):

Liming Guo ◽

Kuibi Tan ◽

Qun Luo ◽

Xu Bai

Keyword(s):

Diabetic Nephropathy ◽

Signaling Pathway ◽

Rat Model ◽

Interstitial Fibrosis ◽

Mtor Signaling ◽

Luciferase Assay ◽

Luciferase Reporter ◽

Mtor Signaling Pathway ◽

Renal Interstitial Fibrosis ◽

Gene Assay

Diabetic nephropathy (DN) is the most common complication of diabetes and is prone to kidney failure. Dihydromyricetin (DHM) has been reported to have a variety of pharmacological activities. This study aims to explore the effect of DHM on DN and the underlying molecular mechanism. An in vivo DN rat model was established. The degree of renal interstitial fibrosis (RIF) was detected by hematoxylin-eosin (HE) staining, Masson's trichrome staining, and immunohistochemistry (IHC). In vitro, NRK-52E cells were divided into four groups: normal glucose (NG), high glucose (HG), HG+DHM, and HG+rapamycin (autophagy inhibitor). The levels of autophagy- and fibrosis-related proteins were analyzed by western blotting. The expression of miR-155-5p and phosphatase and tensin homolog deleted on chromosome ten (PTEN) and their relationship were assessed by quantitative reverse transcription (qRT)-PCR and dual luciferase reporter gene assay. Our results showed that RIF was increased in DN rat model and in HG-induced NRK-52E cells. DHM treatment attenuated the increased RIF and also increased autophagy. MiR-155-5p expression was increased, while PTEN expression was decreased in DN rat and cell model, and DHM reversed both effects. Dual luciferase assay showed that PTEN was the target gene of miR-155-5p. DHM inhibited HG-induced fibrosis and promoted autophagy by inhibiting miR-155-5p expression in NRK-52E cells. In addition, DHM promoted autophagy by inhibiting the PI3K/AKT/mTOR signaling pathway. In conclusion, DHM promotes autophagy and attenuates RIF by regulating the miR-155-5p/PTEN signaling and PI3K/AKT/mTOR signaling pathway in DN.

Download Full-text

Integration Traditional Chinese Medicine and Nibble Debridement and Dressing Method Reduce Thrombosis and Inflammatory Response in the Treatment of Thromboangiitis Obliterans

10.21203/rs.3.rs-107305/v1 ◽

2020 ◽

Author(s):

Jianhua Li ◽

Jingfeng Zhong ◽

Chunfa Huang ◽

Jiewen Guo ◽

Arong Li

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Rat Model ◽

Inflammatory Cell ◽

Blood Rheology ◽

Arterial Disease ◽

Sodium Laurate ◽

Thromboangiitis Obliterans ◽

Dressing Method ◽

Masson Staining

Abstract Background: Thromboangiitis obliterans (TAO), also known as Buerger's disease, is an occlusive arterial disease. However, the pathogenesis of TAO is still unclear. Research has shown that traditional Chinese medicine (TCM) has significant advantages in the treatment of TAO. Our purpose is to explore the underlying roles of TCM in combination with nibble debridement and dressing method (NDDM) in TAO rat model.Methods: 10 mg/ml sodium laurate was utilized to establish a TAO rat model, and then the TAO mode rats were treated with notoginseng powder (NP), maifusheng (MFS) or the combination of NP or MFS and NDDM. Ganrene’s classification and, blood rheology was evaluated; the pathological characteristics of rat limbs were examined by H&E staining and Masson staining; CD3+ and CD20+ levels were measured by immunohistochemistry and flow cytometry. In addition, inflammation-associated cytokines were analyzed by RT-qPCR, Western blot and ELISA assays. Results: ntegration NP or MFS and NDDM dramatically reduced the ganrene’s classification and affected blood rheology parameter of TAO model rats compared with NP and MFS alone. Meanwhile, NP or MFS in combination with NDDM decreased CD3+CD20+T cells, reduced thrombosis and inflammatory cell infiltration, and dramatically decreased the levels of inflammation-associated cytokines.Conclusion: Our results suggested that integration NP or MFS and NDDM could relieve the symptoms of TAO model rats induced by sodium laurate, which might provide new management strategy for TAO.

Download Full-text

Renoprotective effects of pirfenidone on chronic renal allograft dysfunction by reducing renal interstitial fibrosis in a rat model

Life Sciences ◽

10.1016/j.lfs.2019.116666 ◽

2019 ◽

Vol 233 ◽

pp. 116666

Author(s):

Zhen-Zhen Qiu ◽

Ji-Ming He ◽

Hao-Xiang Zhang ◽

Zuo-Hua Yu ◽

Zhi-Wei Zhang ◽

...

Keyword(s):

Rat Model ◽

Renal Allograft ◽

Interstitial Fibrosis ◽

Renal Interstitial Fibrosis ◽

Allograft Dysfunction

Download Full-text

Wenshen Jianpi recipe, a blended traditional Chinese medicine, ameliorates proteinuria and renal injury in a rat model of diabetic nephropathy

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-019-2598-1 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Xiaodan Cao ◽

Renxiong Wei ◽

Jun Zhou ◽

Xiaoxia Zhang ◽

Wenbo Gong ◽

...

Keyword(s):

Diabetic Nephropathy ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Rat Model ◽

Renal Injury

Download Full-text

Preventive Effects of the Intestine Function Recovery Decoction, a Traditional Chinese Medicine, on Postoperative Intra-Abdominal Adhesion Formation in a Rat Model

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/1621894 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Cancan Zhou ◽

Pengbo Jia ◽

Zhengdong Jiang ◽

Ke Chen ◽

Guanghui Wang ◽

...

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Rat Model ◽

Adhesion Formation ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

High Dose ◽

Abdominal Adhesion ◽

Function Recovery ◽

Model Control

The intestine function recovery decoction (IFRD) is a traditional Chinese medicine that has been used for the treatment of adhesive intestinal obstruction. In this study, the preventative effects and probable mechanism of the IFRD were investigated in a rat model. We randomly assigned rats to five groups: normal, model, control, low dose IFRD, and high dose IFRD. In the animal model, the caecum wall and parietal peritoneum were abraded to induce intra-abdominal adhesion formation. Seven days after surgery, adhesion scores were assessed using a visual scoring system, and histopathological samples were examined. The levels of serum interleukin-6 (IL-6) and transforming growth factor beta-1 (TGF-β1) were analysed by an enzyme-linked immunosorbent assay (ELISA). The results showed that a high dose of IFRD reduced the grade of intra-abdominal adhesion in rats. Furthermore, the grades of inflammation, fibrosis, and neovascularization in the high dose IFRD group were significantly lower than those in the control group. The results indicate that the IFRD can prevent intra-abdominal adhesion formation in a rat model. These data suggest that the IFRD may be an effective antiadhesion agent.

Download Full-text