scholarly journals Edaphic factors and plants influence denitrification in soils from a long-term arable experiment

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ian M. Clark ◽  
Qingling Fu ◽  
Maïder Abadie ◽  
Elizabeth R. Dixon ◽  
Aimeric Blaud ◽  
...  

Abstract Factors influencing production of greenhouse gases nitrous oxide (N2O) and nitrogen (N2) in arable soils include high nitrate, moisture and plants; we investigate how differences in the soil microbiome due to antecedent soil treatment additionally influence denitrification. Microbial communities, denitrification gene abundance and gas production in soils from tilled arable plots with contrasting fertilizer inputs (no N, mineral N, FYM) and regenerated woodland in the long-term Broadbalk field experiment were investigated. Soil was transferred to pots, kept bare or planted with wheat and after 6 weeks, transferred to sealed chambers with or without K15NO3 fertilizer for 4 days; N2O and N2 were measured daily. Concentrations of N2O were higher when fertilizer was added, lower in the presence of plants, whilst N2 increased over time and with plants. Prior soil treatment but not exposure to N-fertiliser or plants during the experiment influenced denitrification gene (nirK, nirS, nosZI, nosZII) relative abundance. Under our experimental conditions, denitrification generated mostly N2; N2O was around 2% of total gaseous N2 + N2O. Prior long-term soil management influenced the soil microbiome and abundance of denitrification genes. The production of N2O was driven by nitrate availability and N2 generation increased in the presence of plants.

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 456 ◽  
Author(s):  
Massimiliano Cardinale ◽  
Stefan Ratering ◽  
Aitak Sadeghi ◽  
Sushil Pokhrel ◽  
Bernd Honermeier ◽  
...  

The effects of different agronomic practices, such as fertilization regimes, can be experimentally tested in long-term experiments (LTE). Here, we aimed to evaluate the effect of different nitrogen fertilizations on the bacterial microbiota in both rhizosphere and bulk soil of sugar beet, in the Giessen-LTE (Germany). Fertilization treatments included mineral-N, manure, mineral-N + manure and no N-amendment. Metabarcoding and co-occurrence analysis of 16S rRNA genes, qPCR of amoA, nirK, nirS, nosZ-I and nosZ-II genes and soil physico-chemical analyses were performed. The effect of the fertilization treatments was more evident in the bulk soil, involving 33.1% of the microbiota. Co-occurrence analysis showed a rhizosphere cluster, dominated by Proteobacteria, Actinobacteria and Verrucomicrobia (hub taxa: Betaproteobacteriales), and a bulk soil cluster, dominated by Acidobacteria, Gemmatominadetes and “Latescibacteria” (hub taxa: Acidobacteria). In the bulk soil, mineral N-fertilization reduced nirK, amoA, nosZ-I and nosZ-II genes. Thirteen Operational taxonomic units (OTUs) showed 23 negative correlations with gene relative abundances. These OTUs likely represent opportunistic species that profited from the amended mineral-N and outgrew the species carrying N-cycle genes. Our results indicate trajectories for future research on soil microbiome in LTE and add new experimental evidence that will be helpful for sustainable management of nitrogen fertilizations on arable soils.


Soil Research ◽  
2004 ◽  
Vol 42 (7) ◽  
pp. 737 ◽  
Author(s):  
D. Curtin ◽  
F. M. McCallum

Nitrogen (N) mineralised from soil organic matter can be an important source of N for crop uptake, particularly following cultivation of pastures. Difficulty in predicting the contribution of mineralisation continues to be a serious obstacle to implementating best management practices for fertiliser N. We evaluated biological tests (i.e. net N mineralised in a 28-day aerobic incubation and anaerobically mineralisable N, AMN) and chemical tests (ammonium-N hydrolysis in hot 2 m KCl) as predictors of N supply to a glasshouse-grown oat (Avena sativa L.) crop. The oat plants were grown to maturity without added N on 30 soils representing a range of management histories, including soils collected from long-term pastures and intensive arable cropping sites. The majority (average 83%) of the N accumulated in grain and straw was mineralised N. Plant N derived from mineralisation (PNDM), estimated by subtracting soil mineral N at sowing from N uptake, was generally higher for long-term pasture soils (mean 82 mg/kg, n = 9) than for long-term arable soils (mean 48 mg/kg, n = 9). The 2 measures of N mineralisation were not closely related [R2 = 0.11 (0.37*** when one outlying observation was omitted)], indicating that aerobic and anaerobic assays can give quite different N fertility rankings. Aerobically mineralisable N was the best predictor of PNDM (R2 = 0.79***). The ratio of CO2-C evolved to net N mineralised in the aerobic incubation was highly variable (e.g. mean of 13.6 for pasture soils v. 7.5 for long-term arable soils), likely due to differences in N immobilisation. The correlations of AMN (R2 = 0.32**) and hot KCl N (R2 = 0.24**) with PNDM were not much better than that between total soil N and PNDM (R2 = 0.16*), suggesting that these tests would not provide reliable estimates of N mineralisation potential in soils with diverse management histories.


Holzforschung ◽  
2020 ◽  
Vol 74 (11) ◽  
pp. 1011-1020
Author(s):  
Danyang Tong ◽  
Susan Alexis Brown ◽  
David Corr ◽  
Gianluca Cusatis

AbstractRising global emission have led to a renewed popularity of timber in building design, including timber-concrete tall buildings up to 18 stories. In spite of this surge in wood construction, there remains a gap in understanding of long-term structural behavior, particularly wood creep. Unlike concrete, code prescriptions for wood design are lacking in robust estimates for structural shortening. Models for wood creep have become increasingly necessary due to the potential for unforeseen shortening, especially with respect to differential shortening. These effects can have serious impacts as timber building heights continue to grow. This study lays the groundwork for wood compliance prediction models for use in timber design. A thorough review of wood creep studies was conducted and viable experimental results were compiled into a database. Studies were chosen based on correlation of experimental conditions with a realistic building environment. An unbiased parameter identification method, originally applied to concrete prediction models, was used to fit multiple compliance functions to each data curve. Based on individual curve fittings, statistical analysis was performed to determine the best fit function and average parameter values for the collective database. A power law trend in wood creep, with lognormal parameter distribution, was confirmed by the results.


2000 ◽  
Vol 41 (10-11) ◽  
pp. 117-123 ◽  
Author(s):  
C. Visvanathan ◽  
P. Svenstrup ◽  
P. Ariyamethee

This paper presents a case study of a natural gas production site covering various technical issues related to selection of an appropriate Reverse Osmosis (RO) system. The long-term field experience indicates the necessity of the selection of appropriate pretreatment systems for fouling-free RO operational conditions. The produced water has a variety of impurities such as oil and grease, process chemicals used for corrosion and scaling control, and dehydration of natural gas, etc. This situation leads to a complicated and extremely difficult task for a membrane specialist to design RO systems, especially the pre-treatment section. Here as part of the pretreatment selection, two types of UF membrane modules viz. spiral wound and hollow fibre, with MWCO of 8000 and 50,000 Dalton respectively, were tested in parallel with NF membranes of the spiral wound type with MWCO 200 Dalton. The UF permeate is used as feed for RO compatibility testing. Both configurations of UF failed to be compatible, due to irreversible fouling of the RO membrane. The NF membrane, however, showed interesting results, due to membrane stability in terms of cleaning and fouling. The NF plant with 50% capacity gave a recovery of 75% and the RO plant gave a recovery of 60% versus the expected 92–95%. The long-term tests have indicated that the reminder of the membranes could be installed to achieve full capacity of the plant. This study also demonstrates the importance of selection of proper pre-treatment set-up for the RO system design.


2014 ◽  
Vol 21 ◽  
pp. 1205-1213 ◽  
Author(s):  
Umut Aybar ◽  
Mohammad O. Eshkalak ◽  
Kamy Sepehrnoori ◽  
Tad W. Patzek

SPE Journal ◽  
2021 ◽  
pp. 1-18
Author(s):  
Yingli Xia ◽  
Tianfu Xu ◽  
Yilong Yuan ◽  
Xin Xin ◽  
Huixing Zhu

Summary Natural gas hydrate (NGH) is regarded as an important alternative future energy resource. In recent years, a few short-term production tests have been successfully conducted with both permafrost and marine sediments. However, long-term hydrate production performance and the potential geomechanical problems are not very clear. According to the available geological data at the Mallik site, a more realistic hydrate reservoir model that considers the heterogeneity of porosity, permeability, and hydrate saturation was developed and validated by reproducing the field depressurization test. The coupled multiphase and heat flow and geomechanical response induced by depressurization were fully investigated for long-term gas production from the validated hydrate reservoir model. The results indicate that long-term gas production through depressurization from a vertically heterogeneous hydrate reservoir is technically feasible, but the production efficiency is generally modest, with the low average gas production rate of 4.93 × 103 ST m3/d (ST represents the standard conditions) over a 1-year period. The hydrate dissociation region is significantly affected by the reservoir heterogeneity and reveals a heterogeneous dissociation front in the reservoir. The depressurization production results in significant increase of shear stress and vertical compaction in the hydrate reservoir. The response of shear stress indicates that the potential region of sand migration is mainly in the sand-dominant layer during gas production from the hydraulically heterogeneous hydrate reservoir (e.g., sand layers interbedded with clay layers). The maximum subsidence is approximately 78 mm and occurred at the 72nd day, whereas the final subsidence is slowly dropped to 63 mm after 1-year of depressurization production. The vertical subsidence is greatly dependent on the elastic properties and the permeability anisotropy. In particular, the maximum subsidence increased by approximately 81% when the ratio of permeability anisotropy was set at 5:1. Furthermore, the potential shear failure in the hydrate reservoir is strongly correlated to the in-situ stress state. For the normal fault stress regime, the greater the initial horizontal stress is, the less likely the hydrate reservoir is to undergo shear failure during depressurization production.


2021 ◽  
Author(s):  
Pawan Agrawal ◽  
Sharifa Yousif ◽  
Ahmed Shokry ◽  
Talha Saqib ◽  
Osama Keshtta ◽  
...  

Abstract In a giant offshore UAE carbonate oil field, challenges related to advanced maturity, presence of a huge gas-cap and reservoir heterogeneities have impacted production performance. More than 30% of oil producers are closed due to gas front advance and this percentage is increasing with time. The viability of future developments is highly impacted by lower completion design and ways to limit gas breakthrough. Autonomous inflow-control devices (AICD's) are seen as a viable lower completion method to mitigate gas production while allowing oil production, but their effect on pressure drawdown must be carefully accounted for, in a context of particularly high export pressure. A first AICD completion was tested in 2020, after a careful selection amongst high-GOR wells and a diagnosis of underlying gas production mechanisms. The selected pilot is an open-hole horizontal drain closed due to high GOR. Its production profile was investigated through a baseline production log. Several AICD designs were simulated using a nodal analysis model to account for the export pressure. Reservoir simulation was used to evaluate the long-term performance of short-listed scenarios. The integrated process involved all disciplines, from geology, reservoir engineering, petrophysics, to petroleum and completion engineering. In the finally selected design, only the high-permeability heel part of the horizontal drain was covered by AICDs, whereas the rest was completed with pre-perforated liner intervals, separated with swell packers. It was considered that a balance between gas isolation and pressure draw-down reduction had to be found to ensure production viability for such pilot evaluation. Subsequent to the re-completion, the well could be produced at low GOR, and a second production log confirmed the effectiveness of AICDs in isolating free gas production, while enhancing healthy oil production from the deeper part of the drain. Continuous production monitoring, and other flow profile surveys, will complete the evaluation of AICD effectiveness and its adaptability to evolving pressure and fluid distribution within the reservoir. Several lessons will be learnt from this first AICD pilot, particularly related to the criticality of fully integrated subsurface understanding, evaluation, and completion design studies. The use of AICD technology appears promising for retrofit solutions in high-GOR inactive strings, prolonging well life and increasing reserves. Regarding newly drilled wells, dedicated efforts are underway to associate this technology with enhanced reservoir evaluation methods, allowing to directly design the lower completion based on diagnosed reservoir heterogeneities. Reduced export pressure and artificial lift will feature in future field development phases, and offer the flexibility to extend the use of AICD's. The current technology evaluation phases are however crucial in the definition of such technology deployments and the confirmation of their long-term viability.


2017 ◽  
Vol 23 (2) ◽  
pp. 212-230
Author(s):  
Eric Timperman ◽  
Peter Miksza

The purpose of this study was to examine the effect of verbalization about a brief etude on collegiate string players’ short- and long-term recall of the etude in question. We examined competing hypotheses that suggest it is possible that verbalization (i.e., verbal analysis of musical features) (a) could aid in recall both by highlighting patterns and constraints that inform the music’s creation and by facilitating the creation of explicit performance cues that help to bridge gaps between associative recall chains or (b) may hinder recall by interfering with the creation of procedural and auditory memories necessary for musical performance. Participants ( N = 20) were assigned to experimental conditions in which they learned an unfamiliar etude either through repetition alone or through repetition followed by the completion of a verbalization worksheet provided by the experimenter. Recall was tested both immediately following initial practice and 24 hours later to examine the effect of verbalization on both short- and long-term retention. Findings indicated no differences between groups on immediate recall performance but significant differences at the 24-hour recall task with participants in the verbalization condition recalling more material. In addition, the patterns of errors found across groups indicated a strong primacy effect. Theoretical implications for the study of memory processes in musical contexts and practical implications regarding the preparation of memorized performance are discussed.


2021 ◽  
Vol 248 ◽  
pp. 01040
Author(s):  
Shi Xiaoyan ◽  
Ma Leilei ◽  
Wang Jiantao

Pre-charge as a key step in the battery manufacture processes, which has a great impact on the film-forming properties and electrochemical performances, especially the Li-rich system batteries. As a key influence factor, it is necessary to clarify the effect of pre-charge temperature on battery performance. In this paper, we mainly studied the influence of different pre-charge temperatures (25°C, 40°C, 60°C) on the gas production and electrochemical performance of the batteries. The results show that the increase of the pre-charge temperature will result in the increase of gas production, and the gas components are mainly CO2, H2. After the long-term cycle, the sample under 40°C maintains the highest capacity retention rate, and as the pre-charge temperature increases, the median voltage of the battery can be effectively increased. In addition, compared with room temperature pre-charge, high pre-charge temperature samples have more excellent rate performance.


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