scholarly journals Retraction Note: Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Peng Zhang ◽  
Jian Cui ◽  
Shirin Mansooridara ◽  
Atoosa Shahriyari Kalantari ◽  
Akram Zangeneh ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Aqueous Extract ◽  
Copper Nanoparticles ◽  
Pc12 Cell ◽  
Crocus Sativus ◽  
Pc12 Cell Line ◽  
Leaf Aqueous Extract ◽  
Retraction Notice ◽  
Crocus Sativus L

This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1038/s41598-020-77741-4.

scholarly journals RETRACTED ARTICLE: Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Peng Zhang ◽  
Jian Cui ◽  
Shirin Mansooridara ◽  
Atoosa Shahriyari Kalantari ◽  
Akram Zangeneh ◽  
...  
Keyword(s):  
Cell Death ◽  
Nervous System ◽  
Membrane Potential ◽  
Inflammatory Cytokines ◽  
Aqueous Extract ◽  
Copper Nanoparticles ◽  
Mitochondrial Membrane ◽  
Caspase 3 ◽  
Crocus Sativus ◽  
Leaf Aqueous Extract

AbstractIn this research, we prepared and formulated a neuroprotective supplement (copper nanoparticles in aqueous medium utilizing Crocus sativus L. Leaf aqueous extract) for determining its potential against methadone-induced cell death in PC12. The results of chemical characterization tests i.e., FE-SEM, FT-IR, XRD, EDX, TEM, and UV–Vis spectroscopy revealed that the study showed that copper nanoparticles were synthesized in the perfect way possible. In the TEM and FE-SEM images, the copper nanoparticles were in the mean size of 27.5 nm with the spherical shape. In the biological part of the present research, the Rat inflammatory cytokine assay kit was used to measure the concentrations of inflammatory cytokines. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was used to show DNA fragmentation and apoptosis. Caspase-3 activity was assessed by the caspase activity colorimetric assay kit and mitochondrial membrane potential was studied by Rhodamine123 fluorescence dye. Also, the cell viability of PC12 was measured by trypan blue assay. Copper nanoparticles-treated cell cutlers significantly (p ≤ 0.01) decreased the inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation and they raised the cell viability and mitochondrial membrane potential in the high concentration of methadone-treated PC12 cells. The best result of neuroprotective properties was seen in the high dose of copper nanoparticles i.e., 4 µg. According to the above results, copper nanoparticles containing C. sativus leaf aqueous extract can be used in peripheral nervous system treatment as a neuroprotective promoter and central nervous system after approving in the clinical trial studies in humans.

scholarly journals Development of an Ischemic Tolerance Model in a PC12 Cell Line

2005 ◽  
Vol 25 (2) ◽  
pp. 154-162 ◽  
Author(s):  
Joëlle A Hillion ◽  
Kenzo Takahashi ◽  
Dragan Maric ◽  
Christl Ruetzler ◽  
Jeffery L Barker ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Pc12 Cell ◽  
Annexin V ◽  
Glucose Deprivation ◽  
In Vitro Models ◽  
Ischemic Tolerance ◽  
Cellular Mechanisms ◽  
Pc12 Cell Line

Although ischemic tolerance has been described in a variety of primary cell culture systems, no similar in vitro models have been reported with any cell line. A model of ischemic preconditioning in the rat pheochromocytoma PC12 cell line is described here. When compared to nonpreconditioned cells, preexposure of PC12 cells to 6 hours of oxygen and glucose deprivation (OGD) significantly increased cell viability after 15 hours of OGD 24 hours later. Flow cytometry analysis of cells labeled with specific markers for apoptosis, Annexin V, and Hoechst 33342, and of DNA content, revealed that apoptosis is involved in OGD-induced PC12 cell death and that preconditioning of the cells mainly counteracts the effect of apoptosis. Immunocytochemistry of caspase-3, a central executioner in the apoptotic process, further confirmed the activation of apoptotic pathways in OGD-induced PC12 cell death. This model may be useful to investigate the cellular mechanisms involved in neuronal transient tolerance following ischemia.

The effect of different concentrations of morphine and extracellular Ca2+ on cell death in PC12 cell line

2011 ◽  
Vol 44 (13) ◽  
pp. S179
Author(s):  
Arezou Rabzia ◽  
Mehri Azadbakht ◽  
Ali Bidmeshki Pour ◽  
Hasan Akrami
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Pc12 Cell ◽  
Pc12 Cell Line

Kolaviron inhibits apoptotic cell death and prevents oxidative damage in PC12 cell line exposed to atrazine

2010 ◽  
Vol 196 ◽  
pp. S216
Author(s):  
S. Abarikwu ◽  
M. Kashyap ◽  
I. Adedara ◽  
A. Pant ◽  
E. Farombi
Keyword(s):  
Cell Death ◽  
Oxidative Damage ◽  
Cell Line ◽  
Pc12 Cell ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Pc12 Cell Line

scholarly journals Establishment and Characterization of Methylmercury-Resistant PC12 Cell Line

1994 ◽  
Vol 102 ◽  
pp. 313 ◽  
Author(s):  
Kyoko Miura ◽  
Thomas W. Clarkson ◽  
Kazumasa Ikeda ◽  
Akira Naganuma ◽  
Nobumasa Imura
Keyword(s):  
Cell Line ◽  
Pc12 Cell ◽  
Pc12 Cell Line

scholarly journals Synaptotagmin VII Is Targeted to Dense-core Vesicles and Regulates Their Ca2+-dependent Exocytosis in PC12 Cells

2004 ◽  
Vol 279 (50) ◽  
pp. 52677-52684 ◽  
Author(s):  
Mitsunori Fukuda ◽  
Eiko Kanno ◽  
Megumi Satoh ◽  
Chika Saegusa ◽  
Akitsugu Yamamoto
Keyword(s):  
Plasma Membrane ◽  
Neuropeptide Y ◽  
Cell Line ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Small Interfering Rna ◽  
Dense Core ◽  
Dense Core Vesicles ◽  
Pc12 Cell Line ◽  
Interfering Rna

It has recently been proposed that synaptotagmin (Syt) VII functions as a plasma membrane Ca2+sensor for dense-core vesicle exocytosis in PC12 cells based on the results of transient overexpression studies using green fluorescent protein (GFP)-tagged Syt VII; however, the precise subcellular localization of Syt VII is still a matter of controversy (plasma membraneversussecretory granules). In this study we established a PC12 cell line “stably expressing” the Syt VII-GFP molecule and demonstrated by immunocytochemical and immunoelectron microscopic analyses that the Syt VII-GFP protein is localized on dense-core vesicles as well as in other intracellular membranous structures, such as thetrans-Golgi network and lysosomes. Syt VII-GFP forms a complex with endogenous Syts I and IX, but not with Syt IV, and it colocalize well with Syts I and IX in the cellular processes (where dense-core vesicles are accumulated) in the PC12 cell line. We further demonstrated by an N-terminal antibody-uptake experiment that Syt VII-GFP-containing dense-core vesicles undergo Ca2+-dependent exocytosis, the same as endogenous Syt IX-containing vesicles. Moreover, silencing of Syt VII-GFP with specific small interfering RNA dramatically reduced high KCl-dependent neuropeptide Y secretion from the stable PC12 cell line (∼60% of the control cells), whereas the same small interfering RNA had little effect on neuropeptide Y secretion from the wild-type PC12 cells (∼85–90% of the control cells), indicating that the level of endogenous expression of Syt VII molecules must be low. Our results indicate that the targeting of Syt VII-GFP molecules to specific membrane compartment(s) is affected by the transfection method (transient expressionversusstable expression) and suggested that Syt VII molecule on dense-core vesicles functions as a vesicular Ca2+sensor for exocytosis in endocrine cells.

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