scholarly journals Bacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Phuc N. Ho ◽  
Poramate Klanrit ◽  
Yupa Hanboonsong ◽  
Umaporn Yordpratum ◽  
Manida Suksawat ◽  
...  
Keyword(s):  
Amino Acid ◽  
Defense Mechanism ◽  
Animal Feed ◽  
Glutamate Metabolism ◽  
Nutritional Content ◽  
Bacterial Challenge ◽  
Pyruvate Metabolism ◽  
Hermetia Illucens ◽  
Metabolic Phenotypes ◽  
Organic Acids And Sugars

AbstractBlack soldier fly (BSF, Hermetia illucens) is popular for its applications in animal feed, waste management and antimicrobial peptide source. The major advantages of BSF larva include their robust immune system and high nutritional content that can be further developed into more potential agricultural and medical applications. Several strategies are now being developed to exploit their fullest capabilities and one of these is the immunity modulation using bacterial challenges. The mechanism underlying metabolic responses of BSF to different bacteria has, however, remained unclear. In the current study, entometabolomics was employed to investigate the metabolic phenoconversion in response to either Escherichia coli, Staphylococcus aureus, or combined challenges in BSF larva. We have, thus far, characterised 37 metabolites in BSF larva challenged with different bacteria with the major biochemical groups consisting of amino acids, organic acids, and sugars. The distinct defense mechanism-specific metabolic phenotypes were clearly observed. The combined challenge contributed to the most significant metabolic phenoconversion in BSF larva with the dominant metabolic phenotypes induced by S. aureus. Our study suggested that the accumulation of energy-related metabolites provided by amino acid catabolism is the principal metabolic pathway regulating the defense mechanism. Therefore, combined challenge is strongly recommended for raising BSF immunity as it remarkably triggered amino acid metabolisms including arginine and proline metabolism and alanine, aspartate and glutamate metabolism along with purine metabolism and pyruvate metabolism that potentially result in the production of various nutritional and functional metabolites.

scholarly journals Metabolite Fingerprinting in TransgenicNicotiana tabacumAltered by theEscherichia coliGlutamate Dehydrogenase Gene

2005 ◽  
Vol 2005 (2) ◽  
pp. 198-214 ◽  
Author(s):  
R. Mungur ◽  
A. D. M. Glass ◽  
D. B. Goodenow ◽  
D. A. Lightfoot
Keyword(s):  
Amino Acid ◽  
Cyclotron Resonance ◽  
Ammonium Assimilation ◽  
Ion Cyclotron Resonance ◽  
Glutamate Metabolism ◽  
Dehydrogenase Gene ◽  
Ft Icr Ms ◽  
Organic Acids And Sugars ◽  
Ft Icr ◽  
Ion Analysis

With about 200 000 phytochemicals in existence, identifying those of biomedical significance is a mammoth task. In the postgenomic era, relating metabolite fingerprints, abundances, and profiles to genotype is also a large task. Ion analysis using Fourier transformed ion cyclotron resonance mass spectrometry (FT-ICR-MS) may provide a high-throughput approach to measure genotype dependency of the inferred metabolome if reproducible techniques can be established. Ion profile inferred metabolite fingerprints are coproducts. We used FT-ICR-MS-derived ion analysis to examinegdhA(glutamate dehydrogenase (GDH; EC 1.4.1.1)) transgenicNicotiana tabacum(tobacco) carrying out altered glutamate, amino acid, and carbon metabolisms, that fundamentally alter plant productivity. Cause and effect betweengdhAexpression, glutamate metabolism, and plant phenotypes was analyzed by13NH4+labeling of amino acid fractions, and by FT-ICR-MS analysis of metabolites. ThegdhAtransgenic plants increased13N labeling of glutamate and glutamine significantly. FT-ICR-MS detected 2 012 ions reproducible in 2 to 4 ionization protocols. There were 283 ions in roots and 98 ions in leaves that appeared to significantly change abundance due to the measured GDH activity. About 58% percent of ions could not be used to infer a corresponding metabolite. From the 42% of ions that inferred known metabolites we found that certain amino acids, organic acids, and sugars increased and some fatty acids decreased. The transgene caused increased ammonium assimilation and detectable ion variation. Thirty-two compounds with biomedical significance were altered in abundance by GDH including 9 known carcinogens and 14 potential drugs. Therefore, the GDH transgene may lead to new uses for crops like tobacco.

scholarly journals Responses of Peppermint and Spearmint Crops to Excessive Biostimulant Application and Increased Salinity in a Closed Soilless Production System

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1168
Author(s):  
Dimitrios S. Kasampalis ◽  
Danai-Christina Aktsoglou ◽  
Pavlos Tsouvaltzis ◽  
Anastasios S. Siomos
Keyword(s):  
Amino Acid ◽  
Osmotic Stress ◽  
Antioxidant Defense ◽  
Defense Mechanism ◽  
Substantial Improvement ◽  
Oxygen Species ◽  
Floating System ◽  
High Salt Tolerance ◽  
Heated Glass ◽  
Nutrient Solutions

A floating system was established in a heated glass greenhouse in order to investigate whether the effect of amino acids (0.25 or 0.50% of a commercial amino acid (AA) solution Amino16®) during peppermint and spearmint production on plant developmental and nutritional status may be in part attributed to salinity induced osmotic stress. For this reason, in some nutrient solutions, three levels of salinity were induced by adding 0, 10, or 20 mM NaCl. According to the results, it can be concluded that spearmint is mostly favored by the highest amino acid supplement of the nutrient solution (0.50%) in terms of a substantial improvement of the antioxidant nutritional quality (by up to 130%) at the expense of a reduced biomass production (by <30%). Enzymic antioxidant defense mechanism (APX and POD) was efficiently activated, preventing severe lipid peroxidation and the accumulation of reactive oxygen species such as H2O2 and maintaining the proline content at the normal levels. The osmotic stress that was induced by the excessive AA concentration and confirmed by the chlorophyl fluorescence variations was probably related to NH4+ excess supply in the growing media and was not associated with the elevated electrical conductivity in the solution. The absence of any adverse stressful consequences upon the addition of 20 mM NaCl may be attributed to the high salt tolerance of peppermint and spearmint species.

scholarly journals A high-quality, chromosome-level genome assembly of the Black Soldier Fly (Hermetia illucens L.)

2021 ◽  
Author(s):  
Tomas N Generalovic ◽  
Shane A McCarthy ◽  
Ian A Warren ◽  
Jonathan M D Wood ◽  
James Torrance ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Animal Feed ◽  
Repetitive Sequences ◽  
Genomic Variation ◽  
Runs Of Homozygosity ◽  
High Quality ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Chromosome Conformation ◽  
Important Species

Abstract Hermetia illucens L. (Diptera: Stratiomyidae), the Black Soldier Fly (BSF) is an increasingly important species for bioconversion of organic material into animal feed. We generated a high-quality chromosome-scale genome assembly of the BSF using Pacific Bioscience, 10X Genomics linked read and high-throughput chromosome conformation capture sequencing technology. Scaffolding the final assembly with Hi-C data produced a highly contiguous 1.01 Gb genome with 99.75% of scaffolds assembled into pseudochromosomes representing seven chromosomes with 16.01 Mb contig and 180.46 Mb scaffold N50 values. The highly complete genome obtained a BUSCO completeness of 98.6%. We masked 67.32% of the genome as repetitive sequences and annotated a total of 16,478 protein-coding genes using the BRAKER2 pipeline. We analysed an established lab population to investigate the genomic variation and architecture of the BSF revealing six autosomes and an X chromosome. Additionally, we estimated the inbreeding coefficient (1.9%) of a lab population by assessing runs of homozygosity. This provided evidence for inbreeding events including long runs of homozygosity on chromosome five. Release of this novel chromosome-scale BSF genome assembly will provide an improved resource for further genomic studies, functional characterisation of genes of interest and genetic modification of this economically important species.

Impact of age, size, and sex on adult black soldier fly [Hermetia illucens L. (Diptera: Stratiomyidae)] thermal preference

2021 ◽  
pp. 1-12
Author(s):  
N.F. Addeo ◽  
C. Li ◽  
T.W. Rusch ◽  
A.J. Dickerson ◽  
A.M. Tarone ◽  
...  
Keyword(s):  
Life History ◽  
Thermal Gradient ◽  
Life History Traits ◽  
Animal Feed ◽  
Mass Rearing ◽  
Thermal Preference ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Surface Temperatures ◽  
The Impact

Population growth and rapid urbanisation have increased the global demand for animal feed and protein sources. Therefore, traditional animal feed production should be increased through the use of alternative nutrient sources. Insects as feed are beginning to fill this need. One such insect is the black soldier fly, Hermetia illucens L. (Diptera: Stratiomyidae). However, to more effectively mass produce the black soldier fly, a better understanding of its thermal biology is needed. Thus, the aim of this study was to evaluate the impact of age, size, and sex on adult black soldier fly thermal preference. The thermal preference of adult black soldier flies was determined by exposing flies to a thermal gradient with a range of surface temperatures and monitoring their positions over time. An aluminium plate was used to create a linear thermal gradient where surface temperatures ranged from ~15-60 °C. Flies were distinguished by age (1-d-post-emergence vs 7-d-post-emergence), size (large vs small) and sex (male vs female) to assess whether thermal preference differed by specific life-history traits. Thermal preference for 7-d-post-emergence adults was significantly lower (19.2 °C) than 1-d-post-emergence adults (28.7 °C), respectively. Similarly, small adults selected significantly cooler (21.1 °C) temperatures than large adults (26.9 °C). No significant differences in thermal preferences were found between sex, regardless of age or size. In fact, males and females had similar thermal preference of 23.8 and 24.2 °C, respectively. This study reveals that multiple life-history traits of adult black soldier fly affect their thermal preference, and thus should be taken into consideration by mass rearing facilities to optimize production.

Research and industrialisation of Hermetia illucens L. in China

2020 ◽  
Vol 6 (1) ◽  
pp. 5-12
Author(s):  
J.B. Zhang ◽  
J.K. Tomberlin ◽  
M.M. Cai ◽  
X.P. Xiao ◽  
L.Y. Zheng ◽  
...  
Keyword(s):  
Large Scale ◽  
Organic Waste ◽  
Animal Feed ◽  
Egg Laying ◽  
Breeding Method ◽  
Hermetia Illucens ◽  
Cold Temperatures ◽  
The Usa ◽  
The Government ◽  
Future Work

The larvae of the black soldier fly (BSF), Hermetia illucens L., are commonly associated with decaying organic wastes. Over the past 15 years, investigators in China have conducted extensive research exploring the use of BSF larvae to recycle organic materials as a means to protect the environment, while producing products of value, such as protein and bioenergy. Initial efforts were based on a BSF strain from the USA. However, since then, H. illucens strains from specimens collected in Hubei and Guangdong Provinces have been established and used as models to explore the use of this species in sustainable agriculture. China has played an instrumental role in developing an in-door breeding method using a quartz-iodine lamp rather than depend on natural sunlight. This discovery has allowed the establishment of in-door BSF colonies in regions throughout the world where abiotic conditions (i.e. cold temperatures) are preventative. Researchers in China paved the way for using microbes as a means to enhance BSF production including, enhancing BSF egg-laying as well as waste reduction. Furthermore, bacteria from BSF gut or waste can be cultured and used to promote BSF growth, shorten conversion time, and enhanced conversion efficacy. Recent efforts have demonstrated BSF larvae can degrade antibiotics as well as suppress noxious odours in livestock manure. Due to the efforts of research on BSF in China, numerous companies that recycle organic waste at a large scale (>20 tonnes waste digested/day), have been established. Resulting products include insect powder, and live BSFL that can be used as animal feed ingredients for livestock (e.g. eels and frogs), while protecting the environment. Future work will decipher the mechanisms regulating BSF larval conversion of organic waste so that the system can be optimised. However, efforts are still needed at the government level to establish quality assurance standards if this process is truly to become established as an industry in China.

scholarly journals A Novel Thermostable GH3β-Glucosidase fromTalaromyce leycettanuswith Broad Substrate Specificity and Significant Soybean Isoflavone Glycosides-Hydrolyzing Capability

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Xinxin Li ◽  
Wei Xia ◽  
Yingguo Bai ◽  
Rui Ma ◽  
Hong Yang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Glycoside Hydrolase ◽  
Animal Feed ◽  
Amino Acid Residues ◽  
Calculated Molecular Mass ◽  
Substrate Specificities ◽  
Soybean Isoflavone ◽  
Food Animal ◽  
Isoflavone Glycoside ◽  
Hydrolysis Rates

A novelβ-glucosidase gene (Bgl3B) of glycoside hydrolase (GH) family 3 was cloned from the thermophilic fungusTalaromyce leycettanusJM12802 and successfully expressed inPichia pastoris. The deduced Bgl3B contains 860 amino acid residues with a calculated molecular mass of 91.2 kDa. The purified recombinant Bgl3B exhibited maximum activities at pH 4.5 and 65°C and remained stable at temperatures up to 60°C and pH 3.0−9.0, respectively. The enzyme exhibited broad substrate specificities, showingβ-glucosidase, glucanase, cellobiase, xylanase, and isoflavone glycoside hydrolase activities, and its activities were stimulated by short-chain alcohols. The catalytic efficiencies of Bgl3B were 693 and 104/mM/s towardspNPG and cellobiose, respectively. Moreover, Bgl3B was highly effective in converting isoflavone glycosides to aglycones at 37°C within 10 min, with the hydrolysis rates of 95.1%, 76.0%, and 75.3% for daidzin, genistin, and glycitin, respectively. These superior properties make Bgl3B potential for applications in the food, animal feed, and biofuel industries.

Maggot Black Soldier Fly (Hermetia illucens) Nutritional Content Using Various Culture Media

2021 ◽  
Vol 8 (3) ◽  
pp. 202-211
Author(s):  
Ase Lestari ◽  
T.H Wahyuni ◽  
T.H Wahyuni ◽  
E. Mirwandhono ◽  
N. Ginting
Keyword(s):  
Rice Bran ◽  
Crude Protein ◽  
Culture Media ◽  
Palm Kernel ◽  
Ash Content ◽  
Palm Kernel Cake ◽  
Nutritional Content ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
The Media

Maggot black soldier fly (Hermetia illucens) is an insect that is environmentally friendly and can be mass produced and does not compete with humans. In addition, insect cultivation can reduce organic waste that has the potential to pollute the environment. Maggot black soldier fly (Hermetia illucens) has a high crude protein content of 42.1%. The length of the life cycle and the high and low nutrient content of the maggot are influenced by the maggot growing media used. In this study using EM4 as a bio-activator for fermentation of rice bran, coconut dregs, tofu dregs and palm kernel cake. The function of this fermentation is to increase the nutrients contained in this material which will later be used as a growing medium for maggot and maggot is one of the alternatives to feed protein sources from fish meal. The study was conducted experimentally using a completely randomized design (CRD) 5 treatments and 4 replications, with the weight of each experimental unit the media content is 1 kg and filled with 1 g of BSF eggs and the media size is 37 cm long, 26 cm wide and 10 cm high. The parameters of this study consisted of maggot production and maggot nutritional content, namely moisture content, ash content, crude protein, crude fat and carbohydrates. Based on research results P0 (100% fermented rice bran) obtained an average maggot production of 525g, KA maggot 8,16%, KAb maggot 7,83%, PK maggot 44,82%, LK maggot 21,62% and 17.58% maggot carbohydrates respectively. P1 (50% fermented rice bran + 50% fermented coconut dregs) obtained an average maggot production of 467.5g, KA maggot 9,57%, KAb maggot 9,03%, PK maggot 44,68%, LK maggot 20,51% and 16.20% maggot carbohydrates respectively. P2 (50% fermented rice bran + 50% fermented tofu dregs) obtained an average maggot production of 545.25g, KA maggot 9,02%, KAb maggot 8,32%, PK maggot 44,74%, LK maggot 18,57% and 20.09% maggot carbohydrates respectively. P3 (50% fermented rice bran + 50% palm kernel cake) obtained an average maggot production of 542g, KA maggot 9,16%, KAb maggot 9,22%, PK maggot 43,72%, LK maggot 19,87% and 18.02% maggot carbohydrates respectively. P4 (25% fermented rice bran + 25% fermented tofu dregs + 25% fermented coconut dregs + 25% palm kernel cake) obtained an average maggot production of 831.5g, KA maggot 9,08%, KAb maggot 9,35%, PK maggot 44,38%, LK maggot 17,01% and 20.18% maggot carbohydrates respectively. From the research results, it can be concluded that the use of culture media with a combination of 25% fermented rice bran + 25% fermented coconut dregs + 25% fermented tofu dregs + 25% fermented palm kernel meal is the best combination where the production reaches 831.5 g bsf and 20 carbohydrates. , 18% but could not increase moisture content, ash content, crude protein and crude fat.

Development of high protein soybean experimental lines with enhanced sulfur amino acid content

2019 ◽  
Author(s):  
◽  
Alaa Alaswad
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Nutritive Value ◽  
Animal Feed ◽  
Amino Acid Content ◽  
The United States ◽  
High Protein ◽  
Sulfur Amino Acid ◽  
Soybean Line ◽  
Modest Improvement

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Soybean is one of the most important field crops grown in the United States. Commercial soybeans contain about 36 - 40% protein and 18 - 20% oil, by dry weight. Most of the soybean produced in the U.S. is utilized for oil production and animal feed. Even though soybean is a rich source of protein for livestock and humans, the nutritional quality of soybean can be further enhanced if the concentration of sulfur containing amino acids, methionine and cysteine, can be increased. Previously, our lab has employed transgenic approaches to increase the nutritive value of soybean by expressing heterologous sulfur-rich seed proteins and by manipulating the sulfur assimilatory pathway. Even though modest improvement in the nutritive value has been obtained by these approaches, till now no high yielding soybean cultivars with high protein and high sulfur amino acid content has been developed. Such cultivars will be of great value for the agricultural industry especially in animal feed. In this study, I have crossed a South Korean high protein soybean line (Lee5) with a transgenic soybean line with elevated sulfur amino acid content (CS) and developed soybean experimental lines that were advanced to F4:5 seeds. Theses experimental lines exhibit both high protein and high sulfur amino acid content. The protein content of their seeds has been increased 8 to 14% while their oil content has decreased 5 to 7% when compared with CS. Furthermore, I have successfully introduced the cytosolic isoform of O-acetylserine sulfhydrylase (OASS), the overexpressed transgene, from CS into these crosses. The activity of OASS has been increased 5 to 20-fold in experimental lines when compared to Lee5.

Results of a Study of the Efficiency of Dividing the Hermetia Illucens Larva into Fractions by Amino Acid Composition

2021 ◽  
pp. 41-51
Author(s):  
Dmitry Rudoy ◽  
Viktor Pakhomov ◽  
Arkady Babajanyan ◽  
Tatyana Maltseva ◽  
Polina Mikhailova
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Hermetia Illucens
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