Correlated flickering of erythrocytes membrane observed with dual time resolved membrane fluctuation spectroscopy under different d-glucose concentrations

AbstractA correlated human red blood cell membrane fluctuation dependent on d-glucose concentration was found with dual time resolved membrane fluctuation spectroscopy (D-TRMFS). This new technique is a modified version of the dual optical tweezers method that has been adapted to measure the mechanical properties of red blood cells (RBCs) at distant membrane points simultaneously, enabling correlation analysis. Mechanical parameters under different d-glucose concentrations were obtained from direct membrane flickering measurements, complemented with membrane fluidity measurements using Laurdan Generalized Polarization (GP) Microscopy. Our results show an increase in the fluctuation amplitude of the lipid bilayer, and a decline in tension value, bending modulus and fluidity as d-glucose concentration increases. Metabolic mechanisms are proposed as explanations for the results.

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Cell Palpation System Based on a Force Measurement by Optical Tweezers for Investigation of Local Mechanical Properties of a Cell Membrane

Japanese Journal of Applied Physics ◽

10.1143/jjap.48.120223 ◽

2009 ◽

Vol 48 (12) ◽

pp. 120223 ◽

Cited By ~ 7

Author(s):

Hideaki Miyoshi ◽

Tadao Sugiura ◽

Kotaro Minato

Keyword(s):

Mechanical Properties ◽

Cell Membrane ◽

Optical Tweezers ◽

Force Measurement ◽

A Cell

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Mechanical Properties of Stored Red Blood Cells Using Optical Tweezers

Blood ◽

10.1182/blood.v92.8.2975 ◽

1998 ◽

Vol 92 (8) ◽

pp. 2975-2977 ◽

Cited By ~ 32

Author(s):

R.R. Huruta ◽

M.L. Barjas-Castro ◽

S.T.O. Saad ◽

F.F. Costa ◽

A. Fontes ◽

...

Keyword(s):

Mechanical Properties ◽

Red Blood Cells ◽

Optical Tweezers ◽

Blood Cells

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Mechanical properties of stored red blood cells using optical tweezers

10.1117/12.620758 ◽

2005 ◽

Author(s):

Adriana Fontes ◽

Andre Alexandre de Thomaz ◽

Liliana de Ysasa Pozzo ◽

Maria de Lourdes Barjas-Castro ◽

Marcelo M. Brandao ◽

...

Keyword(s):

Mechanical Properties ◽

Red Blood Cells ◽

Optical Tweezers ◽

Blood Cells

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Measuring electrical and mechanical properties of red blood cells with double optical tweezers

Journal of Biomedical Optics ◽

10.1117/1.2870108 ◽

2008 ◽

Vol 13 (1) ◽

pp. 014001 ◽

Cited By ~ 33

Author(s):

Adriana Fontes ◽

Heloise P. Fernandes ◽

André A. de Thomaz ◽

Luiz C. Barbosa ◽

Maria L. Barjas-Castro ◽

...

Keyword(s):

Mechanical Properties ◽

Red Blood Cells ◽

Optical Tweezers ◽

Blood Cells

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Mechanical Properties of Stored Red Blood Cells Using Optical Tweezers

Blood ◽

10.1182/blood.v92.8.2975.spll4_2975_2977 ◽

1998 ◽

Vol 92 (8) ◽

pp. 2975-2977

Author(s):

R.R. Huruta ◽

M.L. Barjas-Castro ◽

S.T.O. Saad ◽

F.F. Costa ◽

A. Fontes ◽

...

Keyword(s):

Mechanical Properties ◽

Red Blood Cells ◽

Optical Tweezers ◽

Blood Cells

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Measuring electrical and mechanical properties of red blood cells with a double optical tweezers

10.1117/12.680900 ◽

2006 ◽

Author(s):

Adriana Fontes ◽

Heloise P. Fernandes ◽

Maria L. Barjas-Castro ◽

André A. de Thomaz ◽

Liliana d. Y. Pozzo ◽

...

Keyword(s):

Mechanical Properties ◽

Red Blood Cells ◽

Optical Tweezers ◽

Blood Cells

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Characterizing mechanics of membrane tether from relaxation curve obtained by optical tweezers

10.1101/2020.09.06.285353 ◽

2020 ◽

Author(s):

Xuanling Li ◽

Xiaoyu Song ◽

Yinmei Li ◽

Ming Li ◽

Haowei Wang

Keyword(s):

Mechanical Properties ◽

Cell Membrane ◽

Optical Tweezers ◽

Relaxation Curve ◽

Membrane Tension ◽

Protein Diffusion ◽

Relaxation Curves ◽

Membrane Tether ◽

Cell Skeleton ◽

The Relationship

AbstractOptical tweezers is a powerful tool in the study of membrane tension. Comparing to pulling out an entire membrane tether at one time, the step-like method is more efficient because multiple relaxation curves can be obtained from one membrane tether. However, there is few proper models that describe relaxation curves to characterize mechanical properties of cell membrane. Here we established a model to describe the relaxation curve of HeLa cells based on the relationship between membrane tether diameter and tensions. We obtained effective viscosities and static tensions by fitting relaxation curves to our model. We noticed the delicate structure of relaxation curves contains information of cell skeleton changes and protein diffusion. Our study paved a novel pathway to characterize the dynamics and mechanics of cell membrane.

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Non-invasive in Situ Simultaneous Measurement of Multi-parameter Mechanical Properties of Red Blood Cell Membrane

Acta Biochimica et Biophysica Sinica ◽

10.1111/j.1745-7270.2005.00056.x ◽

2005 ◽

Vol 37 (6) ◽

pp. 391-395 ◽

Cited By ~ 4

Author(s):

Jing Li ◽

Yao-Xiong Huang ◽

Tao Ji ◽

Mei Tu ◽

Xuan Mao ◽

...

Keyword(s):

Mechanical Properties ◽

Cell Membrane ◽

Non Invasive ◽

Bending Modulus ◽

Viscoelastic Parameters ◽

Partial Pressures ◽

Different Temperatures ◽

Shear Elasticity ◽

External Conditions

AbstractThe purpose of this study was to develop a new dynamic image analyzing technique that will give us the ability to measure the viscoelastic parameters of individual living red blood cells non-invasively, in situ and in real time. With this technique, the bending modulus KC, the shear elasticity μ and their ratio ɛ were measured under different temperatures, oxygen partial pressures and osmotic pressures. The results not only show the effects of external conditions on mechanical properties of cell membranes including deformability, flexibility, adhesive ability and plasticity, but also demonstrate that the technique can be used to measure cell membrane parameters continuously under several physiological and pathological conditions.

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Single-molecule Force Microscopy: A Powerful Tool for Studying the Mechanical Properties of Cell Membranes

Current Analytical Chemistry ◽

10.2174/1573411017666210602144602 ◽

2021 ◽

Vol 17 ◽

Author(s):

Yan Shi ◽

Mingjun Cai ◽

Hongda Wang

Keyword(s):

Mechanical Properties ◽

Cell Membrane ◽

Optical Tweezers ◽

Single Molecule ◽

Cell Mechanics ◽

Magnetic Tweezers ◽

Adhesion Assay ◽

Force Microscopy ◽

Advantages And Disadvantages ◽

Mechanical Signal

Background: Cell membrane is a physical barrier for cells, as well as an important structure with complex functions in cellular activities. The cell membrane can not only receive external mechanical signal stimulation and respond (e.g., cell migration, differentiation, tumorigenesis, growth), but it can also spontaneously exert force on the environment to regulate cellular activities (such as tissue repair, tumor metastasis, extracellular matrix regulation, etc.). Methods: This review introduces single-molecule force methods, such as atomic force microscopy, optical tweezers, magnetic tweezers, micropipette adhesion assay, tension gauge tethers, and traction force microscopy. Results: This review summarizes the principles, advantages, and disadvantages of single-molecule force methods developed in recent years, as well as their application in terms of force received and generated by cells. The study of cell mechanics enables us to understand the nature of mechanical signal transduction and the manifestation of the cell's movement. Conclusion: The study of the mechanical properties of the cell microenvironment leads to a gradual understanding of the important role of cell mechanics in development, physiology, and pathology. Recently developed combined methods are beneficial for further studying cell mechanics. The optimization of these methods and the invention of new methods enable the continuing research on cell mechanics.

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