scholarly journals Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Konstantin A. Demin ◽  
Tatiana O. Kolesnikova ◽  
David S. Galstyan ◽  
Nataliya A. Krotova ◽  
Nikita P. Ilyin ◽  
...  
Keyword(s):  
Eicosapentaenoic Acid ◽  
Chronic Stress ◽  
Model Organism ◽  
Human Populations ◽  
Omega 3 ◽  
Chronic Unpredictable Stress ◽  
Behavioral Deficits ◽  
Novel Treatments ◽  
Severe Anxiety

AbstractLong-term recurrent stress is a common cause of neuropsychiatric disorders. Animal models are widely used to study the pathogenesis of stress-related psychiatric disorders. The zebrafish (Danio rerio) is emerging as a powerful tool to study chronic stress and its mechanisms. Here, we developed a prolonged 11-week chronic unpredictable stress (PCUS) model in zebrafish to more fully mimic chronic stress in human populations. We also examined behavioral and neurochemical alterations in zebrafish, and attempted to modulate these states by 3-week treatment with an antidepressant fluoxetine, a neuroprotective omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA), a pro-inflammatory endotoxin lipopolysaccharide (LPS), and their combinations. Overall, PCUS induced severe anxiety and elevated norepinephrine levels, whereas fluoxetine (alone or combined with other agents) corrected most of these behavioral deficits. While EPA and LPS alone had little effects on the zebrafish PCUS-induced anxiety behavior, both fluoxetine (alone or in combination) and EPA restored norepinephrine levels, whereas LPS + EPA increased dopamine levels. As these data support the validity of PCUS as an effective tool to study stress-related pathologies in zebrafish, further research is needed into the ability of various conventional and novel treatments to modulate behavioral and neurochemical biomarkers of chronic stress in this model organism.

scholarly journals Modulation of behavioral and hippocampal transcriptomic responses in rat prolonged chronic unpredictable stress model by fluoxetine, eicosapentaenoic acid and lipopolysaccharide

2021 ◽  
Author(s):  
Konstantin A. Demin ◽  
Tatiana O. Kolesnikova ◽  
David S. Galstyan ◽  
Nataliya A. Krotova ◽  
Nikita P. Ilyin ◽  
...  
Keyword(s):  
Eicosapentaenoic Acid ◽  
Chronic Stress ◽  
Anxiety And Depression ◽  
Rodent Models ◽  
Rna Seq ◽  
Chronic Unpredictable Stress ◽  
Treatment Resistant ◽  
Novel Drugs ◽  
Transcriptomic Changes ◽  
Pharmacological Manipulations

AbstractAnimal models are widely used to study common stress-induced affective disorders, such as anxiety and depression. Here, we examine behavioral and brain transcriptomic (RNA-seq) responses in rat prolonged chronic unpredictable stress (PCUS) model, and their modulation by 4-week treatment with fluoxetine, eicosapentaenoic acid (EPA), lipopolysaccharide (LPS) and their combinations. Overall, chronic stress produced anxiety-like phenotype, corrected by fluoxetine alone or in combination with EPA or LPS. EPA was anxiolytic in several tests, whereas LPS alone increased anxiety. PCUS evoked pronounced transcriptomic changes in rat hippocampi, differentially expressing >200 genes, while all pharmacological manipulations (except fluoxetine+EPA) affected only few genes. Gpr6, Drd2 and Adora2a were downregulated by chronic stress in a treatment-resistant manner, suggesting highly conserved nature of these pathogenetic genomic responses to chronic stress. Overall, these findings support the validity of rat PCUS paradigm as an effective tool to study stress-related pathologies, and calls for further research to probe how various conventional and novel drugs modulate behavioral and brain transcriptomic biomarkers of chronic stress in rodent models.

A survey on sleep quality and personality of staffs suffering long-term chronic stress

2014 ◽  
Vol 33 (12) ◽  
pp. 1345-1349
Author(s):  
Wei-zhi LIU ◽  
Yu-qin NONG ◽  
Wei WANG ◽  
Zhi-lei SHANG ◽  
Wen-hua TIAN ◽  
...  
Keyword(s):  
Sleep Quality ◽  
Chronic Stress

scholarly journals Flavonoids Ameliorate Stress-Induced Depression by Preventing NLRP3 Inflammasome Priming

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1231-1231
Author(s):  
Giulio Pasinetti
Keyword(s):  
Mrna Expression ◽  
Chronic Stress ◽  
Nlrp3 Inflammasome ◽  
Signaling Cascades ◽  
Dietary Polyphenols ◽  
Funding Sources ◽  
Glycation End Products ◽  
Molecular Patterns

Abstract Objectives Chronic stress activates danger-associated molecular patterns (DAMPs), stimulating the NLRP3 inflammasome. NLRP3 activation triggers the release of pro-inflammatory cytokine IL-1β. The activity of the NLRP3 inflammasome propagates pro-inflammatory signaling cascades implicated in the onset of depression. Our previous studies show that polyphenolic compounds were found to ameliorate stress induced depression in mouse models. However, the relevant mechanism has not been identified. This study examined the effect of administering polyphenols on DAMP signaling in enriched mice microglia. Methods This study examined the effect of administering polyphenols on DAMP signaling in mice microglia. To recapitulate stress-induced depression, mice underwent chronic unpredictable stress (CUS). Microglia were isolated at various time points throughout the CUS protocol. We also assessed long-term persistent changes after CUS and susceptibility to subthreshold unpredictable stress (US) re-exposure. Results Interestingly, the development of US – induced depression and anxiety depended upon a previous exposure to CUS. We found that CUS caused robust upregulation of IL-1β mRNA in enriched microglia, an effect that persists for up to 4 weeks following CUS exposure. Following the subthreshold US re-exposure, we observed the upregulation of pro- IL-1β as well as pro-receptor for advanced glycation end products (RAGE). Toll-like receptor 4 (TLR-4) was not. We also observed an increase in RAGE mRNA expression when mice were exposed to US prior to the start of the CUS paradigm. Importantly, a primary exposure to US, was sufficient to increase RAGE mRNA expression. We found that polyphenol administration significantly improved CUS-induced depressive-like phenotypes and also reversed neuroinflammation in mice. Treatment with dietary flavonoids prevented upregulation of IL-1β, RAGE mRNA, which reflects the ability of polyphenols that may have begun following the primary exposure to US. Conclusions Taken all together, the results provide evidence of the role of dietary polyphenols in preventing persistent microglial activation, which has been shown to result in reduced long term vulnerability to depressive-like behaviors following expose to chronic stress. Funding Sources This study was supported by a P50 CARBON Center grant from the NCCIH/ODS.

scholarly journals HIGHER DOCOSAHEXAENOIC ACID LEVELS LOWER THE PROTECTIVE IMPACT OF EICOSAPENTAENOIC ACID ON LONG-TERM MACE IN THOSE WITH AND WITHOUT ANGIOGRAPHIC CAD

2021 ◽  
Vol 77 (18) ◽  
pp. 1453
Author(s):  
Viet T. Le ◽  
Stacey Knight ◽  
Kirk Knowlton ◽  
Raymond McCubrey ◽  
Jeramie D. Watrous ◽  
...  
Keyword(s):  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid

scholarly journals Breathing Well Across the Lifespan: Pulmonary Aging and Geroscience-Targeted Therapies

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 749-749
Author(s):  
Jason Sanders
Keyword(s):  
Pulmonary Fibrosis ◽  
Pulmonary Function ◽  
Pulmonary Diseases ◽  
Human Populations ◽  
Aging Research ◽  
Novel Treatments ◽  
Organ Specific ◽  
Nutrient Signaling ◽  
Telomere Biology ◽  
Increased Susceptibility

Abstract Excellent pulmonary function is one of the strongest predictors of longevity across animal models and human populations. Unfortunately, none of the major age-associated pulmonary diseases – obstructive lung disease, pulmonary fibrosis, and increased susceptibility to pneumonia – have strongly effective disease modifying therapies. There is growing evidence that normal age-associated decline in pulmonary function and major age-associated pulmonary diseases are linked to the hallmarks of aging including senescence, nutrient signaling dysregulation, mitochondrial dysfunction, and telomere disorders. This presents opportunities for collaboration between gerontologists and pulmonologists to unravel age-associated developmental mechanisms and design novel treatments. In this symposium, leaders in pulmonary aging research will present novel data on links between aging and pulmonary health and geroscience-based interventions under study. Dr. Sanders will provide an overview of the scientific and clinical space and present epidemiologic associations between aging biomarkers, early pulmonary fibrosis, and mortality. Dr. Le Saux will discuss senescence and specifically how eicosanoid biology may explain organ-specific patterns of senescence-associated fibrosis. Dr. Thannickal will discuss age-associated perturbations in metabolism and mitochondrial function and targeting these pathways to improve lung function and treat pulmonary diseases. Dr. Newton will discuss mechanisms and clinical applications of telomere biology to pulmonary aging. Symposium attendees will (1) be poised to generate collaborations between gerontologists and pulmonologists to address existing knowledge gaps in mechanisms of pulmonary aging, and (2) develop a better understanding of translational opportunities to design geroscience-based diagnostics and therapeutics to improve pulmonary health with aging.

scholarly journals Moody kids years later: Long-term outcomes of youth from the Omega-3 and therapy (OATS) studies

2021 ◽  
Vol 281 ◽  
pp. 24-32
Author(s):  
Mary A. Fristad ◽  
Michelle E. Roley-Roberts ◽  
Sarah R. Black ◽  
L. Eugene Arnold
Keyword(s):  
Omega 3 ◽  
Long Term Outcomes

scholarly journals COVID-19 pandemic related long-term chronic stress on the prevalence of depression and anxiety in the general population

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Tian Qi ◽  
Ting Hu ◽  
Qi-Qi Ge ◽  
Xiao-Na Zhou ◽  
Jia-Mei Li ◽  
...  
Keyword(s):  
Mental Health ◽  
General Population ◽  
Chronic Stress ◽  
Protective Factor ◽  
Mental Health Problems ◽  
Anxiety Symptoms ◽  
Binary Logistic Regression Analysis ◽  
Depression And Anxiety ◽  
Factors Associated

Abstract Background The COVID-19 pandemic has lasted for more than 1 year, causing far-reaching and unprecedented changes in almost all aspects of society. This study aimed to evaluate the long-term consequences of the COVID-19 pandemic on depression and anxiety, and explore the factors associated with it. Methods A cross-sectional study using an online survey was conducted to assess mental health problems from February 2 to February 9, 2021 by using patient health questionnaire-9 (PHQ-9) and generalized anxiety disorder-7 (GAD-7). The insomnia severity index (ISI), demographic data and COVID-19 related variables were measured by a self-designed questionnaire. The factors associated with depressive and anxiety symptoms were identified by Pearson chi-square test and binary logistic regression analysis. Results In the study that 1171 participants enrolled, the overall prevalence of depressive and anxiety symptoms among general people was 22.6 and 21.4% respectively in the present study. Living alone was a potential risk factor for depressive symptoms, while regular exercises was a potential protective factor. The prevalence of depressive and anxiety symptoms was significantly associated with the severity of insomnia symptoms and the negative feelings about pandemic. Conclusion COVID-19 pandemic- related chronic stress has brought about profound impacts on long-term mental health in the general population. The level of insomnia and a negative attitude towards the pandemic are significantly correlated with unfavorable mental health. However, we failed to found a significant association of age and gender with the mental health symptoms, although they were recognized as well-established risk factors during the outbreak by some other studies. This discrepancy may be because the acute and chronic effects of the pandemic are influenced by different factors, which reminds that more attention should be paid to the intrinsic psychological factors and physical reactions towards COVID-19.

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