In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium

AbstractCa-based porous and rough bioceramic surfaces were coated onto zirconium by micro-arc oxidation (MAO). Subsequently, the MAO-coated zirconium surfaces were covered with an antimicrobial chitosan layer via the dip coating method to develop an antimicrobial, bioactive, and biocompatible composite biopolymer and bioceramic layer for implant applications. Cubic ZrO2, metastable Ca0.15Zr0.85O1.85, and Ca3(PO4)2 were detected on the MAO surface by powder-XRD. The existence of chitosan on the MAO-coated Zr surfaces was verified by FTIR. The micropores and thermal cracks on the bioceramic MAO surface were sealed using a chitosan coating, where the MAO surface was porous and rough. All elements such as Zr, O, Ca, P, and C were homogenously distributed across both surfaces. Moreover, both surfaces indicated hydrophobic properties. However, the contact angle of the MAO surface was lower than that of the chitosan-based MAO surface. In vitro bioactivity on both surfaces was investigated via XRD, SEM, and EDX analyses post-immersion in simulated body fluid (SBF) for 14 days. In vitro bioactivity was significantly enhanced on the chitosan-based MAO surface with respect to the MAO surface. In vitro microbial adhesions on the chitosan-based MAO surfaces were lower than the MAO surfaces for Staphylococcus aureus and Escherichia coli.

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In Vitro Biological and Antimicrobial Properties of Chitosan-based Bioceramic Coatings on Zirconium

10.21203/rs.3.rs-208843/v1 ◽

2021 ◽

Author(s):

Salim Levent Aktug ◽

Salih Durdu ◽

Selin Kalkan ◽

Kultigin Cavusoglu ◽

Metin Usta

Keyword(s):

Antimicrobial Properties ◽

Dip Coating ◽

Thermal Cracks ◽

Coating Method ◽

Micro Arc Oxidation ◽

Edx Analyses ◽

Dip Coating Method ◽

The One ◽

Bioceramic Coatings

Abstract Ca-based porous and rough bioceramic surfaces were coated on zirconium by micro arc oxidation (MAO). Subsequently, an antibacterial chitosan layer was covered on the MAO-coated zirconium surfaces by dip coating method to develop an antibacterial, bioactive and biocompatible composite biopolymer and bioceramic layer for implant applications. The cubic-ZrO2, meta-stable Ca0.15Zr0.85O1.85, and Ca3(PO4)2 were detected on the MAO surface by powder-XRD. The existence of chitosan on the MAO-coated Zr surfaces was verified by FTIR. The micro-pores and thermal cracks on the bioceramic MAO surface were sealed by chitosan coating, while the MAO surface is porous and rough. All elements such as Zr, O, Ca, P and C were homogenously distributed through both surfaces. Moreover, both surfaces indicated hydrophobic properties. However, the contact angle value of the MAO surface was lower than the one of chitosan-based MAO surface. In vitro bioactivity on both surfaces was investigated by XRD, SEM and EDX analyses at post-immersion in simulated body fluid (SBF) up to 14 days. In vitro bioactivity was significantly enhanced on the chitosan-based MAO surface with respect to the MAO surface. In vitro bacterial adhesions on the chitosan-based MAO surfaces were lower compared to the MAO surfaces for Staphylococcus aureus and Escherichia coli.

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Biological and antibacterial properties of the micro-nanostructured hydroxyapatite/chitosan coating on titanium

Scientific Reports ◽

10.1038/s41598-019-49941-0 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 10

Author(s):

Baoe Li ◽

Xiaomei Xia ◽

Miaoqi Guo ◽

Yu Jiang ◽

Yu Li ◽

...

Keyword(s):

Composite Coating ◽

Antibacterial Property ◽

Antibacterial Properties ◽

Dip Coating ◽

Ha Coating ◽

Coating Method ◽

Micro Arc Oxidation ◽

Promising Application ◽

Sbf Solution ◽

Dip Coating Method

Abstract Titanium (Ti) is the widely used implant material in clinic, however, failures still frequently occur due to its bioinertness and poor antibacterial property. To improve the biological and antibacterial properties of Ti implants, micro-nanostructured hydroxyapatite (HA) coating was prepared on Ti surface by micro-arc oxidation (MAO), and then the antibacterial agent of chitosan (CS) was loaded on the HA surface through dip-coating method. The results showed that the obtained HA/CS composite coating accelerated the formation of apatite layer in SBF solution, enhanced cell adhesion, spreading and proliferation, and it also inhibited the bacterial growth, showing improved biological and antibacterial properties. Although, with the increased CS amount, the coverage of HA coating would be enlarged, resulting in depressed biological property, however, the antibacterial property of the composite coating was enhanced, and the cytotoxicity about CS was not detected in this work. In conclusion, the HA/CS coating has promising application in orthopedics, dentistry and other biomedical devices.

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Coating Solid Dispersions on Microneedles via a Molten Dip‐Coating Method: Development and In Vitro Evaluation for Transdermal Delivery of a Water‐Insoluble Drug

Journal of Pharmaceutical Sciences ◽

10.1002/jps.24159 ◽

2014 ◽

Vol 103 (11) ◽

pp. 3621-3630 ◽

Cited By ~ 35

Author(s):

Yunzhe Ma ◽

Harvinder S. Gill

Keyword(s):

Transdermal Delivery ◽

Method Development ◽

Solid Dispersions ◽

Dip Coating ◽

In Vitro Evaluation ◽

Coating Method ◽

Water Insoluble Drug ◽

Dip Coating Method

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Biofunctionalization of Textile Materials. 3. Fabrication of Poly(lactide)-Potassium Iodide Composites with Antifungal Properties

Coatings ◽

10.3390/coatings10060593 ◽

2020 ◽

Vol 10 (6) ◽

pp. 593 ◽

Cited By ~ 2

Author(s):

Marcin H. Kudzin ◽

Zdzisława Mrozińska

Keyword(s):

Potassium Iodide ◽

Antimicrobial Properties ◽

Dip Coating ◽

Textile Materials ◽

Nonwoven Fabrics ◽

Antifungal Properties ◽

Ftir Spectrometry ◽

Coating Method ◽

Dip Coating Method ◽

Surface Modified

The paper presents a method of obtaining poly(lactide) (PLA) nonwoven fabrics with antifungal properties using potassium iodide as a nonwoven modifying agent. PLA nonwoven fabrics were obtained by the melt-blown technique and subsequently surface modified (PLA→PLA-SM-KI) by the dip-coating method. The analysis of these PLA-SM-KI (0.1%–2%) composites included Scanning Electron Microscopy (SEM), UV/VIS transmittance, FTIR spectrometry and air permeability. The nonwovens were subjected to microbial activity tests against Aspergillus niger fungal mold species, exhibiting substantial antifungal activity. The studies showed that PLA-KI hybrids containing 2% KI have appropriate mechanical properties, morphology and demanded antimicrobial properties to be further developed as a potential antimicrobial, biodegradable material.

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In Vitro Behavior of Magnesium Apatite Coatings in Organic Modified Simulated Body Fluid

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.299-300.508 ◽

2011 ◽

Vol 299-300 ◽

pp. 508-511

Author(s):

Guo Chao Qi ◽

Feng Jun Shan ◽

Qiang Li ◽

Jing Yuan Yu ◽

Qu Kai Zhang

Keyword(s):

Simulated Body Fluid ◽

Photoelectron Spectroscopy ◽

Body Fluid ◽

Sol Gel ◽

Dip Coating ◽

X Ray ◽

Coating Method ◽

Sbf Solution ◽

Dip Coating Method

Magnesium apatite (MA, (Ca9Mg)(PO4)6(OH)2) and Hydroxyapatite (HA) coatings were synthesized on Ti6Al4V substrates by a sol-gel dip coating method. Glucose and bovine serum albumin (BSA) were added to the standard simulated body fluid (SBF) separately to form organic-containing simulated body fluids. MA and HA coatings were immersed in standard and organic modified SBF for time periods of 4, 7, 14, 21 and 28 days at 37±1°C. The surface dissolution and deposition behavior of the coatings after soaking were examined with Scanning Electron Microscopy (SEM) and X-ray Photoelectron Spectroscopy (XPS). The results show that glucose in SBF has no apparent effect on the deposition of new apatite from the solution. BSA in SBF shows retardation effect on the deposition of apatite by forming a protein dominant globular layer. This layer inhibits the further deposition of apatite from SBF solution.

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Effect of GelMA Hydrogel Coatings on Corrosion Resistance and Biocompatibility of MAO-Coated Mg Alloys

Materials ◽

10.3390/ma13173834 ◽

2020 ◽

Vol 13 (17) ◽

pp. 3834

Author(s):

Wenxian Weng ◽

Weiwei Wu ◽

Xiaoming Yu ◽

Mingyue Sun ◽

Zhensheng Lin ◽

...

Keyword(s):

Corrosion Resistance ◽

Magnesium Alloys ◽

Dip Coating ◽

Crystallographic Structure ◽

X Ray Diffraction ◽

Hydrogel Coatings ◽

Coating Method ◽

Micro Arc Oxidation ◽

Dip Coating Method

Micro-arc oxidation (MAO) treatment is a simple and effective technique to improve the corrosion resistance for magnesium alloys. However, the presence of micro-pores and cracks on the coatings provides paths for corrosive ions to penetrate into and react with the substrate, limiting the long-term corrosion resistance. In this paper, we designed a composite coating with which GelMA hydrogel coatings with varying thicknesses were prepared on the surface of MAO-coated magnesium alloys via a dip-coating method, aiming to improve the biocorrosion resistance and biocompatibility. The surface morphology, the chemical composition of GelMA hydrogels, and the crystallographic structure of magnesium alloys were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD), respectively. The corrosion resistance and biocompatibility of all samples were evaluated through electrochemical and biological experiments. The results demonstrated that the addition of GelMA hydrogel could effectively seal the pores and improve the corrosion resistance and biocompatibility of MAO-coated magnesium alloys, especially for the sample with one layer of GelMA hydrogel, showing high cell proliferation rate, and its current density (Icorr) was two orders of magnitude lower than that of the MAO coating. Besides, the balance mechanism between corrosion and protection was proposed. As a result, the GelMA hydrogel coatings are beneficial to the application of MAO-coated magnesium alloys in bone tissue engineering and other fields.

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Silver Nanoparticle-Coated Polyhydroxyalkanoate Based Electrospun Fibers for Wound Dressing Applications

Materials ◽

10.3390/ma14174907 ◽

2021 ◽

Vol 14 (17) ◽

pp. 4907

Author(s):

Ozlem Ipek Kalaoglu-Altan ◽

Havva Baskan ◽

Timo Meireman ◽

Pooja Basnett ◽

Bahareh Azimi ◽

...

Keyword(s):

Wound Dressing ◽

High Performance ◽

High Surface ◽

High Surface Area ◽

Human Keratinocytes ◽

Dip Coating ◽

Wound Dressings ◽

Coating Method ◽

Dip Coating Method

Wound dressings are high performance and high value products which can improve the regeneration of damaged skin. In these products, bioresorption and biocompatibility play a key role. The aim of this study is to provide progress in this area via nanofabrication and antimicrobial natural materials. Polyhydroxyalkanoates (PHAs) are a bio-based family of polymers that possess high biocompatibility and skin regenerative properties. In this study, a blend of poly(3-hydroxybutyrate) (P(3HB)) and poly(3-hydroxyoctanoate-co-3-hydroxy decanoate) (P(3HO-co-3HD)) was electrospun into P(3HB))/P(3HO-co-3HD) nanofibers to obtain materials with a high surface area and good handling performance. The nanofibers were then modified with silver nanoparticles (AgNPs) via the dip-coating method. The silver-containing nanofiber meshes showed good cytocompatibility and interesting immunomodulatory properties in vitro, together with the capability of stimulating the human beta defensin 2 and cytokeratin expression in human keratinocytes (HaCaT cells), which makes them promising materials for wound dressing applications.

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Porous Gel Coatings Obtained by Phase Separation in ORMOSIL System

MRS Proceedings ◽

10.1557/proc-628-cc7.6 ◽

2000 ◽

Vol 628 ◽

Cited By ~ 1

Author(s):

Kazuki Nakanishi ◽

Souichi Kumon ◽

Kazuyuki Hirao ◽

Hiroshi Jinnai

Keyword(s):

Phase Separation ◽

Reaction Time ◽

Volume Fraction ◽

Sol Gel ◽

Reaction Times ◽

Dip Coating ◽

Coating Solution ◽

Coating Method ◽

Gel Phase ◽

Dip Coating Method

ABSTRACTMacroporous silicate thick films were prepared by a sol-gel dip-coating method accompanied by the phase separation using methyl-trimethoxysilane (MTMS), nitric acid and dimethylformamide (DMF) as starting components. The morphology of the film varied to a large extent depending on the time elapsed after the hydrolysis until the dipping of the coating solution. On a glass substrate, the films prepared by early dipping had inhomogeneous submicrometer-sized pores on the surface of the film. At increased reaction times, relatively narrow sized isolated macropores were observed and their size gradually decreased with the increase of reaction time. On a polyester substrate, in contrast, micrometer-sized isolated spherical gel domains were homogeneously deposited by earlier dippings. With an increase of reaction time, the volume fraction of the gel phase increased, then the morphology of the coating transformed into co-continuous gel domains and macropores, and finally inverted into the continuous gel domains with isolated macropores. The overall morphological variation with the reaction time was explained in terms of the phase separation and the structure freezing by the forced gelation, both of which were induced by the evaporation of methanol during the dipping operation.

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