Author Correction: DNA fingerprinting, fixation-index (Fst), and admixture mapping of selected Bambara groundnut (Vigna subterranea [L.] Verdc.) accessions using ISSR markers system

AbstractAs a new crop in Malaysia, forty-four Bambara groundnut (Vigna subterranea L. verdc.) genotypes were sampled from eleven distinct populations of different origins to explore the genetic structure, genetic inconsistency, and fixation index. The Bambara groundnut, an African underutilized legume, has the capacity to boost food and nutrition security while simultaneously addressing environmental sustainability, food availability, and economic inequalities. A set of 32 ISSRs were screened out of 96 primers based on very sharp, clear, and reproducible bands which detected a total of 510 loci with an average of 97.64% polymorphism. The average calculated value of PIC = 0.243, RP = 5.30, H = 0.285, and MI = 0.675 representing the efficiency of primer set for genetic differentiation among the genotypes. The ISSR primers revealed the number of alleles (Na = 1.97), the effective number of alleles (Ne = 1.38), Nei's genetic diversity (h = 0.248), and a moderate level of gene flow (Nm = 2.26) across the genotypes studied. The estimated Shannon’s information index (I = 0.395) indicates a high level of genetic variation exists among the accessions. Based on Nei’s genetic dissimilarity a UPMGA phylogenetic tree was constructed and grouped the entire genotypes into 3 major clusters and 6 subclusters. PCA analysis revealed that first principal component extracted maximum variation (PC1 = 13.92%) than second principal component (PC2 = 12.59%). Bayesian model-based STRUCTURE analysis assembled the genotypes into 3 (best ΔK = 3) genetic groups. The fixation-index (Fst) analysis narrated a very great genetic diversity (Fst = 0.19 to 0.40) exists within the accessions of these 3 clusters. This investigation specifies the effectiveness of the ISSR primers system for the molecular portrayal of V. subterranea genotypes that could be used for genetic diversity valuation, detection, and tagging of potential genotypes with quick, precise, and authentic measures for this crop improvement through effective breeding schemes.

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GENETIC DIVERSITY IN BAMBARA GROUNDNUT (Vigna subterranean) AS REVEALED BY MOLECULAR WEIGHTS OF THE SEEDS’ PROTEINS

Bangladesh Journal of Plant Breeding and Genetics ◽

10.3329/bjpbg.v30i2.35528 ◽

2018 ◽

Vol 30 (2) ◽

pp. 19-28

Author(s):

A. J. Oludare ◽

J. I. Kioko ◽

A. A. Akeem ◽

A. T. Olumide ◽

K. R. Justina ◽

...

Keyword(s):

Genetic Diversity ◽

Seed Proteins ◽

Electrophoretic Analysis ◽

Protein Characterization ◽

Bambara Groundnut ◽

Vigna Subterranea ◽

Phylogenetic Relatedness ◽

Molecular Weights ◽

National Centre ◽

Standard Marker

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn

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Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽

10.1139/g01-096 ◽

2001 ◽

Vol 44 (6) ◽

pp. 995-999 ◽

Cited By ~ 25

Author(s):

H I Amadou ◽

P J Bebeli ◽

P J Kaltsikes

Keyword(s):

Genetic Diversity ◽

Cluster Analysis ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Geographic Origin ◽

Bambara Groundnut ◽

International Institute ◽

Tropical Agriculture ◽

Vigna Subterranea ◽

Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

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Utilization of bambara groundnut (Vigna subterranea (L.) Verdc.) for sustainable food and nutrition security in semi-arid regions of Zimbabwe

PLoS ONE ◽

10.1371/journal.pone.0204817 ◽

2018 ◽

Vol 13 (10) ◽

pp. e0204817 ◽

Cited By ~ 11

Author(s):

Juliet Mubaiwa ◽

Vincenzo Fogliano ◽

Cathrine Chidewe ◽

Evert Jan Bakker ◽

Anita R. Linnemann

Keyword(s):

Arid Regions ◽

Bambara Groundnut ◽

Vigna Subterranea ◽

Sustainable Food ◽

Food And Nutrition Security ◽

Nutrition Security ◽

Food And Nutrition ◽

Semi Arid

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Genetic Relationships Among Representatives of Dasypyrum, Secale and Triticum Species Revealed with RAPD and ISSR Markers

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4229662 ◽

2014 ◽

Vol 42 (2) ◽

pp. 420-430

Author(s):

Agnieszka GRADZIELEWSKA ◽

Mirosław TYRKA ◽

Justyna LESNIOWSKA-NOWAK ◽

Justyna NAZARUK

Keyword(s):

Gene Diversity ◽

Genetic Relationships ◽

Issr Markers ◽

Genetic Distances ◽

Fixation Index ◽

Triticum Species ◽

The Difference ◽

Bridge Species ◽

Differentiation Gene ◽

Rapd And Issr

In this study the genetic similarity among Dasypyrum, Secale andTriticum species with RAPDs and ISSRs was analyzed. To show a level of similarity between the species, 12 populations of Dasypyrum (11 D. villosum and 1 D. breviaristatum), together with 12 accessions belonging to 3 Secale species and 12 accessions from 4 Triticum species were used. Genetic distances (GD) and bootstrap values were calculated and PCA analysis was conducted to present the relationships among the species. To estimate the genetic structure among and inside genera, as well as population differentiation, gene diversity (He), total genetic variation (Ht) and Wrightâ€™s fixation index (Fst) were computed. The highest values were found in Triticum, within which Ht was equal to 0.332Â±0.023 and Fst was 0.42. It confirmed that the material studied was highly differentiated. Both systems found Dasypyrum more related with Triticum, as compared to Secale. With RAPDs, genetic distance (GD) between Triticum and Dasypyrum was 0.435, respectively 0.460 for Secale vs Dasypyrum. In the case of ISSRs these values were 0.374 and 0.407, respectively. Despite the fact that the difference between the two GD indices was insignificant, one should not exclude the possibility of successful hybridization of Dasypyrum and Secale, especially when using bridge species.

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Tolerance and determinants of drought character descriptors of the Madurese landrace bambara groundnut (Vigna subterranea)

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210731 ◽

2020 ◽

Vol 21 (7) ◽

Author(s):

Siti Fatimah ◽

ARIFFIN ARIFFIN ◽

ARDIARINI NOER RAHMI ◽

KUSWANTO KUSWANTO

Keyword(s):

Cluster Analysis ◽

Drought Stress ◽

Dry Weight ◽

Bambara Groundnut ◽

Stress Index ◽

Nutrient Level ◽

Sensitivity Index ◽

Vigna Subterranea ◽

Genotypic Cluster ◽

Wet Weight

Abstract. Fatimah S, Ariffin, Rahmi AN, Kuswanto. 2020. Tolerance and determinants of drought character descriptors of the Madurese landrace bambara groundnut (Vigna subterranea). Biodiversitas 21: 3108-3116. Bambara groundnut (Vigna subterranea L. Verdc.) is legume of African origin overlooked in Indonesia. It has underdeveloped in Indonesia; for example, in East Java, it is only cultivated in Gresik, Lamongan, and Bangkalan of Madura. This plant can potentially be developed in dry lands, such as Madura as it has the ability to grow and develop well in a dry environment with low nutrient level. At present, there are sparse researches on the selection and determination of the drought-tolerant character descriptors of bambara groundnut in Indonesia. The present study used the expected lines of bambara groundnut selected from local lines of various regions in Indonesia using the nested design. The results showed that the drought stress treatment led to stunted growth of 12 bambara groundnut genotypes, including the number of leaves, plant height, canopy diameter, leaf thickness, number of flowers, number of stems or branches, number of internodes, length of leaf stems, root length, root wet weight, canopy wet weight, canopy dry weight, root dry weight, and leaf chlorophyll content. However, drought leads to a slight increase in the width and length of the stomata opening and leaf proline contents. The cluster analysis based on stress index and sensitivity index can classify 3 expected lines originating from Gresik Regency (G1, G2, G3), falling into the drought stress-tolerant category. Accumulated proline contents cannot be used as a descriptor of tolerance to drought stresses in bambara groundnut since the expected lines with an increase in proline contents in leaves during drought stresses based on the cluster analysis do not fall into the genotypic cluster tolerant to drought stresses.

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Quality Characterization of Biscuits from Blends of Bambara Groundnut (Vigna subterranea), Ground Bean Seed (Macrotyloma) and Moringa Seed (Moringa oleifera) Flour

Asian Food Science Journal ◽

10.9734/afsj/2019/v12i430092 ◽

2019 ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Justina Y. Talabi ◽

Babawande A. Origbemisoye ◽

Beatrice O. Ifesan ◽

Victor N. Enujuigha

Keyword(s):

Protein Content ◽

Wheat Flour ◽

Moringa Oleifera ◽

Pasting Properties ◽

Bambara Groundnut ◽

Vigna Subterranea ◽

Bean Seed ◽

Spread Ratio ◽

Seed Flour ◽

Flour Blends

The nutrient composition and the acceptability of biscuit from composite flours of wheat, Bambara groundnut (Vigna subterranea), Ground bean seed (Macrotyloma) and Moringa seed (Moringa oleifera) were evaluated. Bambara groundnut (Vigna subterranea), Ground bean seed (Macrotyloma) and Moringa seed (Moringa oleifera) were dried, and processed into flour. The flour blends developed was used as a substitute for wheat flour as composite flour. The resulting mixtures were then used to produce biscuits at different ratios of wheat flour to flour blends; 100:0, 90:10, 80:20 and 70:30 level of the flour blends. The pasting properties, proximate composition, minerals, physical (spread ratio, weight, thickness and colour) and sensory properties of the composite biscuit were evaluated. The pasting properties of the flours showed that pasting temperature ranged from 68.50°C - 70.0°C and the peak viscousity range from 101.17 RVU – 207.17 RVU, while Break down (43.0 RVU) was highest in 90% wheat: 10% (Bambara- groundnut-ground bean seed- moringa seed flour) (WFF1). The protein content increased from 12.50% in the control (100% wheat flour) to a range of 14.40% - 16.19% in the biscuits; crude fibre decreased from 2.83 to 2.40 - 1.84%, ash content increased from 1.26% to a range of 1.53 - 2.01%, while carbohydrate and energy value reduced from 69.20 to 65.54 - 63.36% and 384.04 Kcal/100 g to 391.34 - 391.55 Kcal/100 g respectively. As the ratio of blends level increase, the thickness, diameter and weight increased but the spread ratio decreased. In conclusion incorporation of bambara groundnut, ground bean seed and moringa seed flour blends played important role in enhancing the nutritional properties of biscuits through improving their protein content, energy value and mineral elements especially calcium and potassium.

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VARIATION MINERAL COMPOSITIONS OF SOME BAMBARA GROUNDNUT (VIGNA SUBTERRANEA (L.) VERDC.) ACCESSIONS

Recent Research in Science and Technology ◽

10.25081/rrst.2017.9.3355 ◽

2017 ◽

pp. 06-09

Author(s):

M. I. ADEBOLA, M. A. ADELANWA ◽

M. S. MOHAMMED ◽

A. E. ESSON

Keyword(s):

Block Design ◽

Mean Value ◽

Bambara Groundnut ◽

Genetic Enhancement ◽

Vigna Subterranea ◽

Mean Values ◽

Calcium Magnesium ◽

Domestic Use ◽

Industrial Use ◽

Mineral Compositions

The study aimed to assess variation in mineral compositions of some accessions of Bambara groundnut (Vigna subterranea [L.] Verdc.) for domestic use and genetic enhancement. Twenty accessions of Bambara groundnut were planted in a Randomised Complete Block Design (RCBD) with three replications. The five minerals were analysed which include, calcium, magnesium, iron, zinc and manganese. These accessions showed significant (P?0.05) variations across the five mineral analysed. Accession no. 18 had the highest mean for iron (2.89 mg100g-1), Accession no. 12 had the highest mean value for zinc (0.65 mg100g-1) Accession no. 8 had the highest mean value for calcium (217.36 mg100g-1), magnesium (14.29 mg100g-1)and manganese (0.82 mg100g-1). However, accession no. 12 had the lowest mean values in four of the minerals except zinc. This result showed that some of these accessions could be selected for genetic enhancement of minerals, some for domestic use and some for industrial use.

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Perubahan Perilaku Dormansi selama Proses Desikasi pada Benih Kacang Bambara (Vigna subterranea L. Verdc.)

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v48i1.29371 ◽

2020 ◽

Vol 48 (1) ◽

pp. 37-43

Author(s):

Maryati Sari ◽

Satriyas Ilyas ◽

M. Rahmad Suhartanto ◽

Abdul Qadir

Keyword(s):

Seed Dormancy ◽

Bambara Groundnut ◽

Vigna Subterranea ◽

Drying Method ◽

Dormancy Breaking ◽

Hard Seed ◽

Seed Desiccation ◽

Germination Behaviour ◽

Field Emergence ◽

Seed Dormancy And Germination

Bambara groundnut seeds often show unsynchronized and slow germination even though on newly harvested seeds. This might be due to the presence of seed dormancy. Therefore, the objective of this research was to obtain the information on seed dormancy and germination behaviour of bambara groundnut seeds during desiccation. The experiment was arranged in a nested design. Dormancy breaking treatments (untreated, mechanical scarification, soaking in 1% KNO3 for 2 hours, mechanical scarification followed by KNO3 soaking) were nested in each of the desiccation levels (fresh seeds with 54.7% moisture content (mc), desiccated seeds with 44.4%, 18.0%, 15.4%, and 12.1% mc). The results showed that newly harvested seeds were in dormant state. Seed desiccation did not increase the intensity of seed dormancy, even resulted in an increase in field emergence (at 30 days after planting) from 43.9% in fresh seeds to around 70% in dry seed (12-15% mc). Seed desiccation (from 44.4% mc to 12.1% mc) increased the field emergence, although it was inadequate to break the dormancy completely. Meanwhile, seed desiccation tended to increase the GA/ABA ratio, but the seed permeability was decreased. The fact showed that seed desiccation reduced the intensity of dormancy of bambara groundnut seed, therefore, it is suggested to modify the drying method in order to accelerate the increasing of GA/ABA ratio while keeping the testa permeable. Keywords: after-ripening, GA/ABA ratio, hard seed, kacang bogor, permeability

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Studies on the Adaptation of Bambara Groundnut [Vigna subterranea (L.) Verdc] in Owerri Southeastern Nigeria

Agricultural Journal ◽

10.3923/aj.2011.60.65 ◽

2011 ◽

Vol 6 (2) ◽

pp. 60-65

Author(s):

N.I.C. Onwubiko ◽

O.B. Odum ◽

C.O. Utazi ◽

P.C. Poly-Mbah

Keyword(s):

Bambara Groundnut ◽

Vigna Subterranea ◽

Southeastern Nigeria

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