scholarly journals The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Leśków Anna ◽  
Tarnowska Małgorzata ◽  
Szczuka Izabela ◽  
Diakowska Dorota
Keyword(s):  
Malignant Melanoma ◽  
Metabolic Activity ◽  
Human Keratinocytes ◽  
Melanoma Cells ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Limax Maximus ◽  
Active Substances ◽  
Arion Rufus

AbstractMolluscs are one of the sources of biologically active substances, which are now intensively studied, especially for their anti-cancer properties. Malignant melanoma originates from melanocytes, develops very quickly and is associated with poor prognosis. Therefore, the aim of the study was to assess the properties of biologically active compounds in sterile mucus isolated from slugs Limax maximus and Arion rufus. Tested mucus were isolated using the new self-developed method which is safe for the environment and the animal donors. The impact of the mucus on human keratinocytes CCD 1106 KERTr and malignant melanoma cells A-375 was examined using MTT assay and SRB assay, which allowed us to determine the cell metabolic activity and cell number after treating them with slug mucus isolated from Limax maximus and Arion rufus decreased human keratinocytes and melanoma cells metabolic activity as well as manifested properties of reducing the number of cells in both tested cell lines, and therefore can be a source of biologically active substances with anticancer potential.

scholarly journals Research of biologically active compounds of catalpa (Catalpa bignonioides Walt.)

2020 ◽  
pp. 52-58
Author(s):  
O. V. Demeshko ◽  
S. V. Romanova ◽  
V. V. Kovalyov ◽  
I. I. Batiuchenko
Keyword(s):  
Acetic Acid ◽  
Phenolic Compounds ◽  
Chromatographic Analysis ◽  
Skin Diseases ◽  
Biologically Active Compounds ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Active Compounds ◽  
Chromatographic Paper ◽  
Active Substances

Catalpa bignonioides Walt. (catalpa) the family Bignoniaceae – a tree, which originates from North America. Catalpa bark contains resins and tannins. Monoterpene glycosides were found in the leaf. Catalpa seeds contain up to 30% of eleostearic acid. Decoctions are made of the bark of the tree. They serve as a gastric agent and have an anthelmintic effect. Decoction of the bark exhibits bronchodilator action, is used in the complex treatment of cancer. The leaves are used to treat skin diseases. The infusion of fruits has anti-herpetic and hypoglycemic properties. The aim of the work is to investigate biologically active compounds of the leaves of catalpa bignini. The object of the study was the leaves of common catalpa harvested in the summer of 2018 in Sevastopol. Chemical identification reactions and methods of chromatographic analysis were used to determine the major groups of biologically active substances of the leaves of the catalpa (chromatographic paper Filtrak FN No. 4, Munktell, Czech Republic). Chromatography was performed in pre-selected solvent systems: n-butanol‒acetic acid‒water (BAW) (4:1:2) ‒ I direction and 15% acetic acid ‒ II direction. Identification reactions were performed with aqueous and aqueous-alcoholic extracts of catalpa leaves. The qualitative composition of the phenolic compounds was investigated by paper chromatography. The content of the sum of phenolic compounds, hydroxycyclic acids and flavonoids was determined by the spectrophotometric method. Based on the held reactions and chromatographic analysis, by the characteristic fluorescence in the filtered UV light before and after treatment with chromogenic reagents and the values of Rf in the raw material the presence of the following groups of biologically active substances were determined: flavonoids, phenol glycosides, hydroxycoric acids. According to the chromatographic analysis, at least 9 substances of phenolic nature have been detected in the alcohol-aqueous extracts. For the first time, the presence of the following groups of biologically active substances: hydroxycoric acids, phenologic glycosides, flavonoids was determined in the leaves of catalpa. Quantitative content of natural compounds in the leaves of common catalpa: the sum of phenolic compounds (4,76 ± 0,04%), of hydroxycoric acids (3,42 ± 0,02%), of flavonoids (1,88 ± 0,02%). The results of the study will be used in the development of the draft quality control methods for medicinal plant raw materials.

scholarly journals INFLUENCE OF BIOTECHNOLOGICAL FACTORS ON THE YIELD OF BIOLOGICALLY ACTIVE COMPOUNDS FROM SORBUS AUCUPARIA

2021 ◽  
pp. 291-300
Author(s):  
Elena Nikolaevna Sokolova ◽  
Tat'yana Vladimirovna Yuraskina ◽  
Yuliya Aleksandrovna Borshcheva ◽  
Natal'ya Aleksandrovna Fursova ◽  
Anton Yur'yevich Sharikov ◽  
...  
Keyword(s):  
Amino Acids ◽  
Biologically Active Compounds ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Theoretical Research ◽  
Sorbus Aucuparia ◽  
Plant Materials ◽  
Active Compounds ◽  
Wide Range ◽  
Active Substances

Currently, the diet of almost all population groups in Russia is characterized by a deficiency of vitamins, essential amino acids, macro- and micronutrients, as well as biologically active substances (flavonoids, carotenoids, etc.). Replenishment of the lack of these components in the diet of the population due to natural sources of plant origin is an important and actual task of national health care. The rational use of natural components of plant materials containing a wide range of natural biologically active substances using biocatalytic methods, as well as the selection of optimal conditions for obtaining commodity forms of ingredients, will allow to create preventive products that have a beneficial effect on the human body. Theoretical research in the field of promising sources of food and biologically active ingredients among wild species of plant materials were carried out. Biomedical properties of Sorbus aucuparia were described. The technological characteristics of the biologically active substances extraction from dried plant raw materials was investigated. Thus, it was revealed that the degree of dried berries grinding about 0.2–0.8 mm, the hydromodule 1 : 10 and the extraction duration 240 minutes are most effective for the extractive substances yield. The enzymatic complex, allowing the maximum to release biologically valuable components to extract was selected. The amino acid composition of rowanberry with the use of high-performance liquid chromatography was investigated. Produced fermentalizates contain complex of biologically active compounds, including amino acids, vitamins, carotenoids, phenolic substances, that makes these ingredients promising for creation of various foodstuffs to improve quality, nutritional and biological value, taste and other consumer properties.

THE CHEMICAL COMPOSITION OF HELIANTHUS ANNUUS L

2021 ◽  
pp. 62-66
Author(s):  
А. Туреханова ◽  
Қ. Тоштай ◽  
М. Дуйсебаева ◽  
Т. Тұрсонжан ◽  
Ж. Жеңіс
Keyword(s):  
Chemical Composition ◽  
Organic Acids ◽  
Helianthus Annuus ◽  
Production Technology ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Helianthus Annuus L ◽  
Soap Production ◽  
Active Substances

Согласно литературным данным, подсолнечное масло богато полезными веществами, однако, в зависимости от степени очистки масла, количество этих компонентов варьируется. В данном исследовании был изучен дистиллят жирных кислот подсолнечного масла. Согласно полученным данным, более 40% состава кислот приходится на линолевую кислоту с F-витаминной активностью. В большом количестве содержатся стерины, составляющие 44% неомыляемого остатка. Токоферолы составляют 9% неомыляемого остатка, 93% из них приходится на a-токоферол. Helianthus annuus L необходим для приготовления натурального мыла, сочетает в себе способ приготовления медицинского мыла с технологией производства. Экстракт из стебля растения. Это свежее натуральное мыло содержит много полезных микро-и макроэлементов, биологически активные соединения и макро - и микроэлементы растения Helianthus annuus L были определены атомно-эмиссионным методом. Основными из них были K (44,9 мг/г), (377,745 мг/г), Na (3,91 мг/г), (7,56 мг/г) и Mg (3,06 мг/г), (1,495 мг/г). Эти элементы полезны для нашего организма и обладают целебными свойствами. Кроме того, в составе растения H. Annuus L. были определены биологически активные вещества, такие как флавоноиды, алкалоиды, органические кислоты, кумарины и витамины. Эти выявленные данные позволяют получать мыло с высоким качеством, а также целебными свойствами. Helianthus annuus L salt, which is necessary for the preparation of natural soap, combines the method of preparation of medical soap production technology. The extract from the stem of the plant. This fresh natural soap contains many useful micro and macronutrients, biologically active compounds and macro - and microelements of the plant Helianthus annuus L were determined by atomicemission method. Main of them were K (44.9 mg/g), (377.745 mg/g), Na (3.91 mg/g), (7.56 mg/g) and Mg (3.06 mg/g), (1.495 mg/g). These elements are useful for our body and have healing properties. In addition, biologically active substances such as fiavonoids, alkaloids, organic acids, coumarins, and vitamins were found in the composition of the plant H. annuus L. These identied data allow us to obtain soap with a high composition and quality and healing properties

Identification of chemotaxonomic plant markers from the genus Centaurea L.

2020 ◽  
Vol 99 (3) ◽  
pp. 21-28
Author(s):  
Gulzina Asanova ◽  
◽  
Alexandra Dodonova ◽  
Wojciech Push ◽  
◽  
...  
Keyword(s):  
Sesquiterpene Lactones ◽  
Great Variability ◽  
Biologically Active Compounds ◽  
Morphological Features ◽  
Biologically Active ◽  
Active Compounds ◽  
Taxonomic Feature ◽  
Chemotaxonomic Markers ◽  
Species Specific ◽  
Active Substances

The use of chemotaxonomic methods for studying plants allows you to help identify the ancestral and species affiliation of closely related plants. Chemotaxonomy is especially useful for systematically young groups of plant taxa, characterized by great variability in morphological features, difficulty in identifying and clarifying systematic affiliation. The article analyses the content of various groups of biologically active compounds in plants of the genus Centaurea L. to find the most convenient chemotaxonomic markers. As a result of the analysis of the variability of flavonoids, sesquiterpene lactones, polysaccharides and other biologically active substances of the genus Centaurea, it was found that «flavonoid profiles» are most species-specific, and sesquiterpene lactones can serve as an additional taxonomic feature at the level of subgenera and sections of this genus. More detailed studies are needed to map the distribution of biologically active compounds as chemotaxonomic markers.

scholarly journals THEORETICAL SUBSTANTIATION OF DIRECT TRANSPORT OF BIOLOGICALLY ACTIVE COMPONENTS IN CONDITIONS OF MODELED GASTROINTESTINAL TRACT

Food systems ◽  
2018 ◽  
Vol 1 (2) ◽  
pp. 21-28
Author(s):  
A. V. Evteev ◽  
N. V. Gorbunova ◽  
O. S. Larionova ◽  
A. V. Bannikova
Keyword(s):  
Small Intestine ◽  
Biologically Active Compounds ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Active Components ◽  
Active Compounds ◽  
Theoretical Substantiation ◽  
Theoretical Approaches ◽  
Active Substances

In this paper, the results of studies on the release of biological active compounds from their encapsulated forms under conditions of enzymatic hydrolysis in vitro are presented. In the phase of the model «small intestine» swelling of the capsules and their subsequent decay occurs, which allows to speak about the controlled release of encapsulated bioactive components. It was revealed that almost 90 % of the residual quantity of essential ingredients was released from the capsules in the model phase of the artificial «small intestine». At the end of the experiment, the capsules released all the encapsulated biologically active substances, regardless of the content of fish oil and phenolic compounds in them. It was noted that the poly-capsules had the greatest propensity to withstand the aggressive environment of the «model stomach» and concentrate in themselves the maximum amount of biologically active substances. Mathematical modeling confirms the direct transport of biologically active compounds and the role of the swelling of capsules in the release of biologically active compounds. two mathematical models describing the classical theory of diffusion from capsules and incorporating the material relaxation coefficient demonstrate a combination of empirical and theoretical approaches in controlling the properties of encapsulated biologically active substances. the obtained data are promising in the field of development of improved and functional food products, as well as the dry ingredients and concentrates 

scholarly journals Extraction of biologically active compounds from the residues of pericarps of sugar beet (Beta vulgaris L. ssp. vulgaris)

2019 ◽  
Vol 25 ◽  
pp. 243-246
Author(s):  
O. L. Klyachenko ◽  
L. A. Janse ◽  
A. F. Likhanov
Keyword(s):  
Phenolic Compounds ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Multicomponent System ◽  
Biologically Active Compounds ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Growth Promoters ◽  
Active Compounds ◽  
Active Substances

Aim. In the regulation of germination of seeds and of growth and development of seedlings, an important role belongs to biologically active substances that are found in the root of sugar beet (Beta vulgaris L. ssp. vulgaris), mainly in the pericarp. Extraction of secondary metabolites and study of the influence of a multicomponent system of endometabolites (phenolic compounds) of sugar beet pericarps on germination of plant seeds. Methods. The chemical composition of the metabolites of fruits and pericarps of sugar beet was determined by thin-layer chromatography; their differentiated isolation was carried out using column chromatography and gel filtration; The total content of phenolic compounds in fruits and pericarps was determined spectrophotometrically. Results. It was found that sugar beet pericarps contain a considerable amount of phenolic compounds (5-10 %). Four groups of phenolic compounds with different molecular masses were detected. By studying the influence of aquatic extracts on radish seeds, it was found that the eluents of the first volumes of water (3-5 ml) showed a pronounced stimulating effect, whereas further volumes of eluents (8-9 ml) were characterized by inhibitory action. Conclusions. Phenols, flavonoids and their glycosides were detected in extracts of sugar beet pericarps, which can be characterized as highly active inhibitors and growth promoters, therefore they could be used as natural biologically active substances. Keywords: sugar beet, pericarp, biologically active compounds, phenols, flavonoids.

Nickel(0)-Catalyzed Linear-Selective Hydroarylation of Unactivated Alkenes and Styrenes with Arylboron Acids

2018 ◽  
Author(s):  
Honggui Lv ◽  
Li-Jun Xiao ◽  
Dongbing Zhao ◽  
Qi-Lin Zhou
Keyword(s):  
Butyric Acid ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Highly Efficient ◽  
Organoboronic Acids

Herein, we realized the first linear-selective hydroarylation of unactivated alkenes and styrenes with organoboronic acids by introducing directing groupon alkenes. Our method is highly efficient and scalable, and provides a modular route to assemble structurally diverse alkylarenes, especially for γ-aryl butyric acid derivatives, which have been widely utilized as chemical feedstocks to access multiple marketed drugs, and biologically active compounds.<br>

SOME DIRECTIONS IN THE MODIFICATION OF AZOLES

2020 ◽  
Vol 5 (443) ◽  
pp. 85-91
Author(s):  
Ibrayev M.K., ◽  
◽  
Takibayeva A.T., ◽  
Fazylov S.D., ◽  
Rakhimberlinova Zh.B., ◽  
...  
Keyword(s):  
13C Nmr Spectroscopy ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Synthesis Conditions ◽  
Alkaloid Cytisine ◽  
Azole Ring ◽  
Cyclic Compounds ◽  
New Compounds ◽  
Derivatives Of

This article presents studies on the targeted search for new derivatives of azoles, such as benzthiazole, 3,5-dimethylpyrazole, 1,3,4-oxadiazole-2-thione, 1,3,4-thiadiazole. The possibility of combining in one molecule of the azole ring with other cyclic compounds: the alkaloid cytisine, morpholine, furan and some arenes has been studied. To obtain new compounds, the reactions of bromination, acylation, and interaction with isothiocyanates were studied. Optimal synthesis conditions were studied for all reactions. It was found that the reaction of 4-bromo-3,5-dimethylpyrazole with isothiocyanates, in contrast to the previously written derivatives of anilines, takes a longer time and requires heating the reaction mixture. The combination of a pirasol fragment with halide substituents often results in an enhanced therapeutic effect. The synthesized 2-bromine-N-(6-rodanbenzo[d]thiazole-2-yl)acetamide, due to the alkylbromide group, is an important synth in the synthesis of new benzthiazole derivatives. Its derivatives combine in one molecule the rest of rhodanbenzthiazole with alkaloid cytisine and biogenic amine morpholine and are potentially biologically active compounds, since the molecule structure contains several pharmacophoric fragments: benzthiazole and alkaloid (amine) heterocycles, rhodane and urea groups. The mechanism of formation of 1,3,4-oxadiazole-2-tyons from hydrazides under action on them by carbon disulfide was studied and assumed. It was shown that dithiocarbamates in acidic medium decompose with the release of hydrogen sulfide and the formation of highly reactive isothiocyanate group. Then, intra-molecular cyclization occurs, with the formation of end products - 1,3,4-oxadiazole-2-thions. The structures of the synthesized compounds were studied by 1H and 13C NMR spectroscopy. All synthesized substances are potentially biologically active compounds, since they contain several pharmacophore fragments in their structure.

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