scholarly journals The immunomodulatory effects of long-term supplementation with Lactobacillus casei Shirota depend on ovalbumin presentation in BALB/c mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Li-Han Chen ◽  
Chun-Hsu Pan ◽  
Shih-Yi Huang ◽  
Ching-Hung Chan ◽  
Hui-Yu Huang
Keyword(s):  
Body Weight ◽  
Antigen Presentation ◽  
Lactobacillus Casei ◽  
Intranasal Administration ◽  
Specific Ige ◽  
Immunomodulatory Effects ◽  
Lactobacillus Casei Shirota ◽  
Fermented Beverage ◽  
High Doses ◽  
Th1 Cytokines

AbstractImmunomodulation is an ability of several particular probiotics. However, it still remains unclear whether the immunomodulatory effects of specific probiotics vary for different antigen presentation models with the same antigen. To investigate this matter, six groups of BALB/c mice (n = 10) were exposed to one of two antigen presentation models: ovalbumin (OVA) by injection alone, or injection plus intranasal administration. Moreover, the mice were fed distilled water or Lactobacillus casei Shirota fermented beverage (LcSFB) at low (2.5 × 109 CFU/kg body weight) or high doses (5 × 109 CFU/kg body weight) by gavage for 8 weeks. LcSFB enhanced the proliferation of splenocytes, production of OVA-specific immunoglobulin (Ig)-G and IgA, and the ratio of T-helper (Th)-2/Th1 cytokines in mice injected with OVA. Conversely, in the mice treated with OVA by injection plus intranasal administration, LcSFB attenuated the immune responses against OVA by reducing the proliferation of splenocytes, levels of OVA-specific IgE, IgG, and IgM, and ratio of Th2/Th1 cytokines. Moreover, LcSFB increased the percentage of regulatory T cells in the injection plus intranasal administration group. Taken together, this work indicates the immunoregulatory effects of LcSFB depend on how the antigen is presented. Therefore, the use of probiotics to boost the immune system must be carefully considered.

The probiotic Lactobacillus casei Shirota attenuates symptoms of vestibular migraine: a randomised placebo-controlled double-blind clinical trial

2020 ◽  
Vol 11 (5) ◽  
pp. 469-476
Author(s):  
X. Qi ◽  
G. Fan ◽  
H. Jia
Keyword(s):  
Depressive Symptoms ◽  
Lactobacillus Casei ◽  
Daily Basis ◽  
Vestibular Migraine ◽  
Double Blind ◽  
Beneficial Effects ◽  
Lactobacillus Casei Shirota ◽  
Probiotic Lactobacillus ◽  
Vestibular Symptoms

Vestibular migraine (VM) has emerged as one of the major vestibular syndromes. Although probiotics have exhibited beneficial effects on migraine headache, its effect on VM is not clear. This trial aimed to investigate the treatment efficacy of the probiotic Lactobacillus casei Shirota (LcS) on symptoms of VM. 247 VM patients were enrolled, of which 204 eligible patients receiving either LcS or placebo on a daily basis completed the 4 month study. They were re-visited at 2 and 4 months after study initiation to assess treatment outcomes. The primary endpoints were vestibular symptoms, evaluated by the number of vertiginous attacks during the past week, the Vertigo Severity Score (VSS), and Dizziness Handicap Inventory (DHI) scores. The secondary endpoints were anxiety and depressive symptoms, evaluated using Beck Anxiety Inventory (BAI) and Beck Depression Inventory (BDI) scores. Efficacy of LcS over placebo was not obvious at 2 month follow-up. At 4 month follow-up, while both LcS and placebo groups of VM patients displayed alleviated symptoms, the extents of the improvements were significantly better in LcS group than those of placebo group, with regard to vestibular symptoms using DHI and VSS, as well as anxiety and depressive symptoms using BAI and BDI. Although placebo effect cannot be ignored in intervention for VM patients, the probiotic LcS still exhibits considerable efficacy against VM symptoms over a 4 month study period, supporting further clinical study of a larger and more diverse cohort.

scholarly journals Oral administration of Lactobacillus casei Shirota can ameliorate the adverse effect of an acute aflatoxin exposure in Sprague Dawley rats

2018 ◽  
Vol 88 (3-4) ◽  
pp. 199-208
Author(s):  
Elham Nikbakht ◽  
Rosita Jamaluddin ◽  
S. Mohd Redzwan ◽  
Saman Khalesi
Keyword(s):  
Adverse Effect ◽  
Lactobacillus Casei ◽  
Health Effect ◽  
Acute Exposure ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Lactobacillus Casei Shirota ◽  
Probiotic Treatment ◽  
Significant Difference ◽  
Aflatoxin B

Abstract. Aflatoxin B1 (AFB1) is a toxic compound commonly found in some crops with an adverse health effect on human and animals. Some beneficial microorganisms (or probiotics) such as lactic acid bacteria have shown the ability to reduce the bioavailability of aflatoxins and its intestinal absorption. However, the dose and duration of aflatoxins exposure and probiotic treatment can influence the ability of probiotics to remove aflatoxins. Therefore, this research aimed to investigate the efficacy of oral probiotic Lactobacillus casei Shirota strain (LcS) induction in an acute exposure to AFB1 in rats. Experimentally, Sprague Dawley rats were divided into three groups: AFB1 only (n = 9); AFB1 treated with LcS (n = 9); and control (no AFB1 exposure) (n = 6) groups. The blood AFB1 level of rats treated with LcS was slightly lower than the untreated AFB1 induced rats (11.12 ± 0.71 vs 10.93 ± 0.69 ng g–1). Also, LcS treatment slightly moderated the liver and kidney biomarkers in AFB1 induced rats. However, a trend for a significant difference was only observed in ALT of AFB1 induced rats treated with LcS compared to their counterparts (126.11 ± 36.90 vs 157.36 ± 15.46, p = 0.06). Rats’ body weight decreased in all animals force-fed with AFB1 with no significant difference between LcS treatment compared to the counterpart. In conclusion, this experiment indicated that probiotic LsC was able to slightly ameliorate the adverse effect of an acute exposure to AFB1 in rats. However, future studies with longer probiotics treatment or higher probiotics dose is required to confirm these findings.

The effect of probiotic treatment on elderly patients with distal radius fracture: a prospective double-blind, placebo-controlled randomised clinical trial

2016 ◽  
Vol 7 (5) ◽  
pp. 631-637 ◽  
Author(s):  
M. Lei ◽  
L-M. Hua ◽  
D-W. Wang
Keyword(s):  
Clinical Trial ◽  
Elderly Patients ◽  
Distal Radius Fracture ◽  
Distal Radius ◽  
Lactobacillus Casei ◽  
Radius Fracture ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Lactobacillus Casei Shirota ◽  
Probiotic Treatment

Probiotic treatment has been shown to improve bone formation, increase bone mass density and prevent bone loss. We aimed to assess the effect of probiotic treatment on functional recovery in elderly patients with a distal radius fracture. A total of 417 elderly patients with an acute distal radius fracture were enrolled in this double-blind placebo-controlled clinical trial. They were randomised to receive skimmed milk containing either a commercial probiotic (Lactobacillus casei Shirota) or placebo daily for a period of 6 months after the fracture. Treatment outcomes were the DASH (disabilities of the arm, shoulder and hand) score, pain, complex regional pain syndrome (CRPS) score, active range of motion and grip strength, all of which were measured on a monthly basis. Throughout the duration of the study, DASH score, pain, CRPS score, wrist flexion and grip strength of patients receiving probiotics exhibited a significantly faster pace of improvement than those on placebo, with treatment outcomes of patients receiving Lactobacillus casei Shirota at month 4 at comparable levels with those of patients receiving placebo at month 6. In elderly patients with a fracture of the distal radius, administration of the probiotic could greatly accelerating the healing process.

scholarly journals Immunomodulatory Effects of Lactobacillus casei Administration in a Mouse Model of Gliadin-Sensitive Enteropathy

2011 ◽  
Vol 74 (4) ◽  
pp. 335-341 ◽  
Author(s):  
R. D’Arienzo ◽  
R. Stefanile ◽  
F. Maurano ◽  
G. Mazzarella ◽  
E. Ricca ◽  
...  
Keyword(s):  
Mouse Model ◽  
Lactobacillus Casei ◽  
Immunomodulatory Effects

ÁCIDOS LÁTICO E ACÉTICO OBTIDOS A PARTIR DA FERMENTAÇÃO DO LEITE COM Euterpe oleracea Mart. (AÇAÍ) POR Lactobacillus casei Shirota

2020 ◽  
pp. 36-43
Author(s):  
Adriele Torquato Rodrigues ◽  
Adriellen Oliveira Branco ◽  
Rithely de Aquino dos Santos ◽  
Victória Cristiane Moura Coelho ◽  
Edailson de Alcântara Corrêa
Keyword(s):  
Lactobacillus Casei ◽  
Euterpe Oleracea ◽  
Lactobacillus Casei Shirota ◽  
Euterpe Oleracea Mart

scholarly journals Effects of Nitrofurantoin on the Primary and Secondary Reproductive Organs of Female B6C3F Mice

1989 ◽  
Vol 17 (4_part_2) ◽  
pp. 774-781 ◽  
Author(s):  
Katherine A. Stitzel ◽  
R. Frank McConnell ◽  
T. A. Dierckman
Keyword(s):  
Body Weight ◽  
Time Course ◽  
Body Weight Gain ◽  
Reproductive Organs ◽  
Ovarian Tumors ◽  
Feed Consumption ◽  
Mouse Strains ◽  
Carcinogenicity Study ◽  
High Doses ◽  
Benign Ovarian Tumors

The National Toxicology Program (NTP) has reported female mice fed high doses of Nitrofurantoin (NFT) were found to have ovarian atrophy as diagnosed histologically and increased benign ovarian tumors after 24 months of exposure (30). This result contrasts with 4 other recent carcinogenicity assays in rodents with NFT, all with no evidence of an ovarian effect. An extensive database documents benign tubular adenomas develop secondary to ovarian atrophy in many mouse strains, including B6C3F1 (see ref 11). The present study was initiated to confirm this mechanism could be responsible for the ovarian tumors in the NTP study and to investigate the time course of ovarian changes seen in female B6C3F1 mice. Mice were provided diet containing NFT at doses of 350 and 500 mg/kg body weight/day and examined after 4, 8, 13, 17, 43 and 64 weeks. A dose-related decrease in feed consumption, feed efficiency and body weight gain was seen and persisted throughout the study. Sexual maturity was delayed in a dose-related fashion, compatible with previously reported effects of reduced food consumption in rodents (12, 16). All groups of mice eventually did have normal estrous cycles, but cycle lengths were increased in a dose-related fashion. Both doses of NFT resulted in histological evidence of senile ovarian atrophy by week 43. Based on the reported association between sterility and ovarian tumors, we conclude the benign tubular adenomas seen at 2 yr in the NTP carcinogenicity study with NFT were secondary to the ovarian atrophy induced in this strain of mouse and not an indication NFT, itself, is a carcinogen.

scholarly journals Bactericidal activity against intermediately cephalosporin-resistant Streptococcus pneumoniae in cerebrospinal fluid of children with bacterial meningitis treated with high doses of cefotaxime and vancomycin.

1997 ◽  
Vol 41 (9) ◽  
pp. 2050-2052 ◽  
Author(s):  
C Doit ◽  
J Barre ◽  
R Cohen ◽  
S Bonacorsi ◽  
A Bourrillon ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Body Weight ◽  
Streptococcus Pneumoniae ◽  
Bacterial Meningitis ◽  
Bactericidal Activity ◽  
Additive Interaction ◽  
High Doses ◽  
Resistant Pneumococcus

Cerebrospinal fluid (CSF) was taken from 19 children with bacterial meningitis treated with cefotaxime (300 mg/kg of body weight/day) and vancomycin (60 mg/kg/day). Median levels of drugs in CSF were smaller than expected, as follows: 4.4 microg/ml for cefotaxime, 3.2 microg/ml for desacetylcefotaxime, and 1.7 microg/ml for vancomycin. The median CSF bactericidal titer against an intermediately cefotaxime-resistant pneumococcus was 1:4. Our data suggest at least an additive interaction between the drugs used in this study.

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