scholarly journals Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hamish C. L. Yau ◽  
Adam K. Malekpour ◽  
Nazarmohammad G. Momin ◽  
Ana L. Morales-García ◽  
William G. T. Willats ◽  
...  
Keyword(s):  
High Resolution ◽  
Extracellular Polymeric Substances ◽  
Dnase I ◽  
Extracellular Dna ◽  
Molecular Probe ◽  
Effective Removal ◽  
Resolution Imaging ◽  
Cleaning Technology ◽  
First Time

AbstractWashed textiles can remain malodorous and dingy due to the recalcitrance of soils. Recent work has found that ‘invisible’ soils such as microbial extracellular DNA (eDNA) play a key role in the adhesion of extracellular polymeric substances that form matrixes contributing to these undesirable characteristics. Here we report the application of an immunostaining method to illustrate the cleaning mechanism of a nuclease (DNase I) acting upon eDNA. Extending previous work that established a key role for eDNA in anchoring these soil matrixes, this work provides new insights into the presence and effective removal of eDNA deposited on fabrics using high-resolution in-situ imaging. Using a monoclonal antibody specific to Z-DNA, we showed that when fabrics are washed with DNase I, the incidence of microbial eDNA is reduced. As well as a quantitative reduction in microbial eDNA, the deep cleaning benefits of this enzyme are shown using confocal microscopy and imaging analysis of T-shirt fibers. To the best of our knowledge, this is the first time the use of a molecular probe has been leveraged for fabric and homecare-related R&D to visualize eDNA and evaluate its removal from textiles by a new-to-laundry DNase enzyme. The approaches described in the current work also have scope for re-application to identify further cleaning technology.

Development of a translation stage for in situ noninvasive analysis and high-resolution imaging

inArt 2016 ◽  
2017 ◽  
pp. 185-189
Author(s):  
David Strivay ◽  
Mathieu Clar ◽  
Said Rakkaa ◽  
Francois-Philippe Hocquet ◽  
Catherine Defeyt
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Noninvasive Analysis ◽  
Translation Stage ◽  
Resolution Imaging

scholarly journals Galactography with Tomosynthesis Technique (Galactomosynthesis) – Renaissance of a Method?

2018 ◽  
Vol 78 (05) ◽  
pp. 493-498 ◽  
Author(s):  
Rüdiger Schulz-Wendtland ◽  
Caroline Preuss ◽  
Peter Fasching ◽  
Christian Loehberg ◽  
Michael Lux ◽  
...  
Keyword(s):  
High Resolution ◽  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Imaging Technique ◽  
Nipple Discharge ◽  
Full Field ◽  
Contrast Enhanced ◽  
First Time

Abstract Introduction For decades, conventional galactography was the only imaging technique capable of showing the mammary ducts. Today, diagnosis is based on a multimodal concept which combines high-resolution ultrasound with magnetic resonance (MR) mammography and ductoscopy/galactoscopy and has a sensitivity and specificity of up to 95%. This study used tomosynthesis in galactography for the first time and compared the synthetic digital 2D full-field mammograms generated with this technique with the images created using the established method of ductal sonography. Both methods should be able to detect invasive breast cancers and their precursors such as ductal carcinoma in situ (DCIS) as well as being able to identify benign findings. Material and Methods Five patients with pathological nipple discharge were examined using ductal sonography, contrast-enhanced 3D galactography with tomosynthesis and the synthetic digital 2D full-field mammograms generated with the latter method. Evaluation of the images created with the different imaging modalities was done by three investigators with varying levels of experience with complementary breast diagnostics (1, 5 and 15 years), and their evaluations were compared with the histological findings. Results All 3 investigators independently evaluated the images created with ductal sonography, contrast-enhanced 3D galactography with tomosynthesis, and generated synthetic digital 2D full-field mammograms. Their evaluations were compared with the histopathological assessment of the surgical specimens resected from the 5 patients. There was 1 case of invasive breast cancer, 2 cases with ductal carcinoma in situ and 2 cases with benign findings. All 3 investigators made more mistakes when they used the standard imaging technique of ductal sonography to diagnose suspicious lesions than when they used contrast-enhanced galactography with tomosynthesis and the generated synthetic digital 2D full-field mammograms. Conclusion This is the first time breast tomosynthesis was used in galactography (galactomosynthesis) to create digital 3-dimensional images of suspicious findings. When used together with the generated synthetic digital 2D full-field mammograms, it could be a useful complementary procedure for the diagnosis of breast anomalies and could herald a renaissance of this method. Compared with high-resolution ductal ultrasound, the investigators achieved better results with contrast-enhanced galactography using tomosynthesis and the generated synthetic digital 2D full-field mammograms, as confirmed by histopathological findings.

Non-invasive high-resolution acoustic microscopy technique using embedded nanostructures

2007 ◽  
Vol 1019 ◽  
Author(s):  
Daniel Wulin ◽  
Shriram Ramanathan
Keyword(s):  
High Resolution ◽  
Acoustic Waves ◽  
High Frequency ◽  
Acoustic Microscopy ◽  
Biological Applications ◽  
Acoustic Oscillations ◽  
Non Invasive ◽  
Microscopy Technique ◽  
First Time

AbstractAn opto-acoustic system capable of operating at frequencies greater than 1 GHz with novel biological applications is proposed for the first time. Metallic spheres with radii on the order of hundreds of nanometers dispersed inside a bio-matrix can be used to generate in-situ ultra-high frequency acoustic waves whose normal mode frequencies can be calculated using Lamb's theory for acoustic oscillations of elastic spheres. The frequency and amplitude of the resulting acoustic waves can be related to the physical properties of the metallic spheres and the surrounding bio-matrix: the acoustic waves produced by the metallic spheres are well-suited to high resolution acoustic imaging. We anticipate that our approach will open up new nanoscale techniques to study cells non-invasively.

High resolution imaging of mitochondrial membranes by in situ atomic force microscopy

RSC Advances ◽  
2013 ◽  
Vol 3 (3) ◽  
pp. 708-712 ◽  
Author(s):  
Yongmei Tian ◽  
Jiahan Li ◽  
Mingjun Cai ◽  
Weidong Zhao ◽  
Haijiao Xu ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
High Resolution Imaging ◽  
Mitochondrial Membranes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Resolution Imaging

High-Resolution Dynamic Analysis of the Phase Transformation in Ge2Sb2Te5 Alloy

2007 ◽  
Vol 26-28 ◽  
pp. 1199-1202
Author(s):  
Se Ahn Song ◽  
Wei Zhang ◽  
Hong Sik Jeong ◽  
Jin Gyu Kim ◽  
Youn Joong Kim
Keyword(s):  
Phase Transformation ◽  
Crystal Growth ◽  
High Resolution ◽  
Large Scale ◽  
Voltage Electron ◽  
Transmission Electron ◽  
In Situ Heating ◽  
First Time ◽  
Crystal Growth Behavior

Phase transformation and crystal growth behavior of Ge2Sb2Te5 were investigated systematically by means of in situ heating (from room temperature to 500 oC) of amorphous Ge2Sb2Te5 alloy in a high voltage electron microscope with real-time monitoring. Large-scale crystallization occurred to amorphous Ge2Sb2Te5 around 200 oC. Large crystal growth developed on heating from 200 oC to 400 oC, and single crystalline grains grew up to 150 nm. Eventually the onset of partial melting of thin Ge2Sb2Te5 foil was at 500 oC and liquid Ge2Sb2Te5 was observed for the first time by high-resolution transmission electron microscopy. Hexagonal Ge2Sb2Te5 phase remains after a subsequent cooling.

ISDoT: in situ decellularization of tissues for high-resolution imaging and proteomic analysis of native extracellular matrix

2017 ◽  
Vol 23 (7) ◽  
pp. 890-898 ◽  
Author(s):  
Alejandro E Mayorca-Guiliani ◽  
Chris D Madsen ◽  
Thomas R Cox ◽  
Edward R Horton ◽  
Freja A Venning ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
High Resolution ◽  
Proteomic Analysis ◽  
High Resolution Imaging ◽  
Resolution Imaging
Sign in / Sign up

Export Citation Format

Share Document