Sibutramine reduces feeding, body fat and improves insulin resistance in dietary-obese male Wistar rats independently of hypothalamic neuropeptide Y

Introduction: The neuropeptide Y (NPY) in the neural circuits of the hypothalamus has a stimulating effect on reproductive activities in mammals. Kisspeptin (KiSS1) is a quintessential neurotransmitter in the reproductive axis which directly stimulates gonadotropin-releasing hormone neurons in the hypothalamus. The distribution of KiSS1 expressing cells in the pituitary was described previously. Despite earlier reports showing the KiSS1 receptor, G-protein coupled receptor 54 (GPR54) expression in the pituitary, the potential physiological roles of kisspeptin at this gland have remained obscure. Accordingly, this study investigated the role of NPY on the relative expression of Kiss1 and Gpr54 genes in the pituitary gland in male Wistar rats. Methods: In general, 20 male Wistar rats weighing 200-250 g in 4 groups (5 in each group) received saline, NPY (2.3 nM), BIBP3226 (NPY receptor antagonist, 7.8 nM), and NPY+ BIBP3226. Then, they received the simultaneous injection of these molecules through the third ventricle of the brain. Finally, the relative mean expressions of Kiss1 and Gpr54 genes in the anterior pituitary were quantitatively analyzed by the real-time polymerase chain reaction. Results: The central injection of NPY increased the relative mean expressions of Kiss1 and Gpr54 genes in the pituitary gland compared to the control group although the injection of BIBP3226 eradicated these effects. However, the gene expression of Gpr54 in the rats receiving NPY coupled with BIBP3226 in hypophysis in comparison to the group receiving only NPY demonstrated a significant reduction (P<0.05). Conclusion: Overall, the central injection of NPY stimulated the gene expression of Kiss1 and Gpr54 in the pituitary gland.

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Curcumin prevents inflammatory response, oxidative stress and insulin resistance in high fructose fed male Wistar rats: Potential role of serine kinases

Chemico-Biological Interactions ◽

10.1016/j.cbi.2015.12.012 ◽

2016 ◽

Vol 244 ◽

pp. 187-194 ◽

Cited By ~ 30

Author(s):

Nachimuthu Maithilikarpagaselvi ◽

Magadi Gopalakrishna Sridhar ◽

Rathinam Palamalai Swaminathan ◽

Bobby Zachariah

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Inflammatory Response ◽

Wistar Rats ◽

Potential Role ◽

High Fructose ◽

Male Wistar Rats ◽

Serine Kinases

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Effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats

10.21203/rs.3.rs-336533/v1 ◽

2021 ◽

Author(s):

Michael A. Olamoyegun ◽

Folasade O. Ajao ◽

Marcus O. Iyedupe

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Lipid Profile ◽

Wistar Rats ◽

Metabolic Diseases ◽

Restricted Feeding ◽

Mean Values ◽

Male Wistar Rats ◽

Ad Libitum ◽

Obese Male

Abstract Background: Obesity greatly increases the risk of metabolic diseases and preventive approaches for obesity are often inadequate to effectively prevent and manage the diseases. Altering feeding time strategy intervention decreases caloric intake without calorie counting and may be an effective therapy. Therefore, this study investigates the effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats. Methods: Rats placed on time-restricted feeding (TRF) regimen had freely access to food for 4 hour per day at designated periods. Twenty four rats divided into four groups (n=6) were used. Group I animals were placed on a 4 hour per day TRF between 8am-12noon. Group II rats were also placed on a 4 hour per day TRF between 12noon-4pm. Group III rats also placed on a 4 hour per day TRF between 8pm-12 midnight while Group IV rats had access food and water ad libitum. This diet strategy resembles taking only breakfast, lunch or dinner once a day. The study lasted for a period of 4 weeks with daily food intake and weekly body weight determined throughout the period. At the end of the experimental period, blood glucose, lipid profile and leptin concentration were assessed. SPSS 21.0 package was used for data analysis, one-way analysis of variance (ANOVA) was used to compare the mean values of variables among the groups and bonferroni’s posthoc test was used for significance of pair wise comparisons of mean values among the groups. Significance was set at p < 0.05.Results: In this study, the body weights and leptin concentrations of 8pm – 12am and ad libitum groups significantly increased compared with the 8am - 12noon and 12noon -4pm groups. Dyslipidemia was observed in the ad libitum group when compared with the 8am - 12noon and 12noon - 4pm groups. Conclusion: From this study, 4-hr time restricted feeding has beneficial effects on body weight, blood glucose, lipid profile and leptin concentration. This feeding restriction patterns may be helpful in obesity management and in preventing metabolic diseases development in non obese.

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Protective role of melatonin against adipose-hepatic metabolic comorbidities in experimentally induced obese rat model

PLoS ONE ◽

10.1371/journal.pone.0260546 ◽

2021 ◽

Vol 16 (12) ◽

pp. e0260546

Author(s):

Mary J. Obayemi ◽

Christopher O. Akintayo ◽

Adesola A. Oniyide ◽

Ayodeji Aturamu ◽

Olabimpe C. Badejogbin ◽

...

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Food Intake ◽

High Fat Diet ◽

Wistar Rats ◽

Liver Lipid ◽

Control Group ◽

Metabolic Dysfunction ◽

High Fat ◽

Male Wistar Rats

Background Adipose and hepatic metabolic dysfunctions are critical comorbidities that also aggravate insulin resistance in obese individuals. Melatonin is a low-cost agent and previous studies suggest that its use may promote metabolic health. However, its effects on some comorbidities associated with obesity are unknown. Herein, we investigated the hypothesis that melatonin supplementation would attenuate adipose-hepatic metabolic dysfunction in high fat diet (HFD)-induced obesity in male Wistar rats. Materials and methods Twenty-four adult male Wistar rats (n = 6/group) were used: Control group received vehicle (normal saline), obese group received 40% high fat diet, melatonin-treated group received 4 mg/kg of melatonin, and obese plus melatonin group received 40% HFD and melatonin. The treatment lasted for 12 weeks. Results HFD caused increased food intake, body weight, insulin level, insulin resistance and plasma and liver lipid but decreased adipose lipid. In addition, HFD also increased plasma, adipose and liver malondialdehyde, IL-6, uric acid and decreased Glucose-6-phosphate dehydrogenase, glutathione, nitric oxide and circulating obestatin concentration. However, these deleterious effects except food intake were attenuated when supplemented with melatonin. Conclusion Taken together, the present results indicate that HFD exposure causes adipose-hepatic metabolic disturbance in obese animals, which are accompanied by oxidative stress and inflammation. In addition, the present results suggest that melatonin supplementation attenuates adipose-hepatic metabolic dysfunction, accompanying obesity by suppression of oxidative stress/inflammation-dependent mechanism and increasing circulating obestatin.

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