scholarly journals Effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats

2021 ◽  
Author(s):  
Michael A. Olamoyegun ◽  
Folasade O. Ajao ◽  
Marcus O. Iyedupe
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Restricted Feeding ◽  
Mean Values ◽  
Male Wistar Rats ◽  
Ad Libitum ◽  
Obese Male

Abstract Background: Obesity greatly increases the risk of metabolic diseases and preventive approaches for obesity are often inadequate to effectively prevent and manage the diseases. Altering feeding time strategy intervention decreases caloric intake without calorie counting and may be an effective therapy. Therefore, this study investigates the effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats. Methods: Rats placed on time-restricted feeding (TRF) regimen had freely access to food for 4 hour per day at designated periods. Twenty four rats divided into four groups (n=6) were used. Group I animals were placed on a 4 hour per day TRF between 8am-12noon. Group II rats were also placed on a 4 hour per day TRF between 12noon-4pm. Group III rats also placed on a 4 hour per day TRF between 8pm-12 midnight while Group IV rats had access food and water ad libitum. This diet strategy resembles taking only breakfast, lunch or dinner once a day. The study lasted for a period of 4 weeks with daily food intake and weekly body weight determined throughout the period. At the end of the experimental period, blood glucose, lipid profile and leptin concentration were assessed. SPSS 21.0 package was used for data analysis, one-way analysis of variance (ANOVA) was used to compare the mean values of variables among the groups and bonferroni’s posthoc test was used for significance of pair wise comparisons of mean values among the groups. Significance was set at p < 0.05.Results: In this study, the body weights and leptin concentrations of 8pm – 12am and ad libitum groups significantly increased compared with the 8am - 12noon and 12noon -4pm groups. Dyslipidemia was observed in the ad libitum group when compared with the 8am - 12noon and 12noon - 4pm groups. Conclusion: From this study, 4-hr time restricted feeding has beneficial effects on body weight, blood glucose, lipid profile and leptin concentration. This feeding restriction patterns may be helpful in obesity management and in preventing metabolic diseases development in non obese.

scholarly journals The influence of food restriction versus ad libitum feeding of chow and purified diets on variation in body weight, growth and physiology of female Wistar rats

2012 ◽  
Vol 46 (2) ◽  
pp. 101-107 ◽  
Author(s):  
M Moraal ◽  
P P A M Leenaars ◽  
H Arnts ◽  
K Smeets ◽  
B S Savenije ◽  
...  
Keyword(s):  
Body Weight ◽  
Food Restriction ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Blood Parameters ◽  
Calorie Intake ◽  
Restricted Feeding ◽  
Organ Weights ◽  
Female Wistar Rats ◽  
Ad Libitum

Ad libitum (AL) supply of standard chow is the feeding method most often used for rodents in animal experiments. However, AL feeding is known to result in a shorter lifespan and decreased health as compared with restricted feeding. Restricted feeding and thus limiting calorie intake prevents many health problems, increases lifespan and can also increase group uniformity. All this leads to a reduced number of animals needed. So-called standard chows are known to be prone to variation in composition. Synthetic foods have a more standard composition, contributing to group uniformity which, like diet reduction, may decrease the number of animals necessary to obtain statistical significance. In this study, we compared the effects of AL versus restricted feeding (25% reduction in food intake) on standard chow versus synthetic food of three different suppliers on body weight (BW), growth, several blood parameters and organ weights in growing female Wistar rats over a period of 61 days. Diet restriction led to a decreased growth and significantly reduced variation in BW and growth as compared with AL feeding. AL feeding on synthetic diets caused a significantly higher BW gain than on chow diets. Due to experimental design, this same effect occurred on food restriction. Blood parameters and organ weights were affected neither by diet type nor by amount. Incidentally, variations were significantly reduced on food restriction versus AL, and on synthetic diets versus chow diets. This study demonstrates that food restriction versus AL feeding leads to a significantly reduced variation in BW and growth, thereby indicating the potential for reduction when applying this feeding schedule.

scholarly journals The supplementation effect of Red Dragon fruit’s skin extract on the fasting blood glucose and lipid profiles in male Wistar rats with diabetes mellitus and dyslipidemia

2020 ◽  
Vol 3 (3) ◽  
pp. 87-92
Author(s):  
Stefani Marietta ◽  
AAG Budhiarta ◽  
I Wayan Weta
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Mean Difference ◽  
Control Group ◽  
Skin Extract ◽  
Before And After ◽  
Male Wistar Rats ◽  
Mean Differences

Background: Flavonoids, saponins, tannins, phenols, and vitamin-C contained in the Red Dragon fruit’s skin have a positive impact on glycemic control and lipid oxidation. This study aimed to determine the effect of Red Dragon fruit’s skin extract on reducing the fasting blood glucose (FBG) and improving the lipid profile of Wistar rats with diabetes and dyslipidemia. Methods: A randomized pre-test post-test control group experimental study was done on 22 male Wistar rats, aged 2-3 months that suffered from diabetes and dyslipidemia. Subjects were divided into the control group (given 2cc distilled water + 9 mg metformin) and the treatment group (given 160 mg red dragon fruit’s skin extract + 9 mg metformin) for 14 days. FBG and lipid profile measurements were done before and after the treatment. Data were analyzed using the compare mean test. Results: There was no significant mean difference of GDP between groups before (p=0.414) and after treatment (p=0.125), total cholesterol between groups before (p = 0.572) and after treatment (p=0.361), triglycerides between groups before (p=0.073) and after treatment (p=0.111). There was a significant mean difference of HDL between groups before (p=0.003) and after treatment (p=0.047), LDL between groups before (p=0.006) and after treatment (p=0.043). Although there were significant mean differences in HDL and LDL between groups before and after treatment, the pre-post treatment of HDL and LDL mean differences showed no significant mean difference (p=0.328 and p=0.704 consecutively). Conclusion: Red Dragon fruit’s skin extract treatment did not significantly reduce the mean FBG and lipid profile levels.

The Effects of Food Deprivation of Rats On Swimming To Exhaustion

Behaviour ◽  
1966 ◽  
Vol 26 (3-4) ◽  
pp. 351-356 ◽  
Author(s):  
Edward T. Uyeno ◽  
R. Alan Graham
Keyword(s):  
Body Weight ◽  
Food Deprivation ◽  
Wistar Rats ◽  
Control Group ◽  
Dorsal Skin ◽  
The Third ◽  
Male Wistar Rats ◽  
Ad Libitum ◽  
Fourth Experiment ◽  
Ad Libitum Feeding

AbstractMale Wistar rats were deprived of food for 3 days and then forced to swim continuously to a criterion of exhaustion. Controls swam after ad libitum feeding. In the first experiment a load equal to 11% of the rat's body weight was attached to the dorsal skin near the tail during the swim. In a second study, two matched groups, treated identically as those in the first study swam without a load in water treated with a wetting agent, "Aerosol O.T." In a third experiment, controls as well as experimentals were deprived of food for three days. The control group, however, was fed for 30 minutes, immediately before the swim. A fourth experiment was conducted in a similar manner as the third, except both groups were deprived 6 hours. In each of the experiments the deprived groups swam significantly longer than the fed groups.

Intermittent exposure to green and white light-at-night activates hepatic glycogenolytic and gluconeogenetic activities in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Abayomi O. Ige ◽  
Olubori S. Adekanye ◽  
Elsie O. Adewoye
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Blood Glucose ◽  
Wistar Rats ◽  
Light At Night ◽  
Group Iii ◽  
Group I ◽  
Intermittent Exposure ◽  
Male Wistar Rats ◽  
Group Ii

Abstract Objectives Exposure to light-at-night (LAN) has been reported to impair blood glucose regulation. The liver modulates blood glucose through mechanisms influenced by several factors that include peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1α) and glucose-6-phosphatase (G6Pase). This study investigated the effect of intermittent exposure to green and white LAN on some hepatic glucose regulatory factors in male Wistar rats. Methods Animals were divided into three equal groups. Group I (control) was exposed to normal housing conditions. Groups II and III were each daily exposed to either green or white LAN for 2 h (7–9 pm) for 14 days. Body weight and blood glucose was monitored on days 0, 7, and 14. Thereafter, retro-orbital sinus blood was obtained after light thiopental anaesthesia and serum insulin was determined. Liver samples were also obtained and evaluated for glycogen, PGC-1α, and G6Pase activity. Insulin resistance was estimated using the HOMA-IR equation. Results Body weight and blood glucose on days 7 and 14 increased in groups II and III compared to control. Hepatic PGC-1α and G6Pase increased in group II (2.33 ± 0.31; 2.07 ± 0.22) and III (2.31 ± 0.20; 0.98 ± 0.23) compared to control (1.73 ± 0.21; 0.47 ± 0.11). Hepatic glycogen was 71.8 and 82.4% reduced in groups II and III compared to control. Insulin in group II increased (63.6%) whiles group III values reduced (27.3%) compared to control. Insulin resistance increased in group II (0.29 ± 0.09) compared to control (0.12 ± 0.03) and group III (0.11 ± 0.03), respectively. Conclusions Exposure to 2 h green and white LAN in the early dark phase increases hepatic glycogenolysis and gluconeogenetic activities resulting in increased blood glucose. In male Wistar rats, exposure to green but not white LAN may predispose to insulin resistance.

Qualitative regional differences in adipose tissue growth and cellularity in male Wistar rats fed ad libitum

1998 ◽  
Vol 274 (5) ◽  
pp. R1460-R1467 ◽  
Author(s):  
M. DiGirolamo ◽  
J. B. Fine ◽  
K. Tagra ◽  
R. Rossmanith
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Regional Differences ◽  
Wistar Rats ◽  
Cell Number ◽  
Tissue Growth ◽  
Fat Cell ◽  
Fat Depots ◽  
Male Wistar Rats ◽  
Ad Libitum

Adipose tissue grows primarily by a combination of increases in fat cell volume (hypertrophy) and in fat cell number (hyperplasia), but the regional growth pattern of white adipose tissue depots in animal species and in the human is still unclear. In this study we characterized fully the age-related changes in adipose tissue growth, composition, and cellularity of four fat depots of male Wistar rats that varied in age from 7 wk to 15 mo and in body weight from 178 to 808 g. Body weight and the weight of each of the four adipose depots studied (epididymal, mesenteric, subcutaneous inguinal, and retroperitoneal) increased progressively with age and ad libitum feeding. Comparison of the cellularity of the four adipose depots, however, showed remarkable and significant differences in the pattern of growth within the same animals. The cumulative growth of the two intra-abdominal fat depots (mesenteric and epididymal) was due mostly to hypertrophy (increases in cell volume of 83 and 64%, respectively), whereas the growth of the other two depots (retroperitoneal and inguinal) was due predominantly to hyperplasia (increases in cell number of 58 and 65%, respectively). These findings uncover major and unexpected regional differences in the modulation of adipose tissue growth within aging animals fed ad libitum and suggest local, region-specific regulatory controls of this growth.

scholarly journals A Comparative study on Efficacy of Lutein and Atorvastatin on Lipid Profile and Lipoprotein(A) in Hypercholesterolemic male Wistar Rats

2021 ◽  
Vol 14 (01) ◽  
pp. 503-511
Author(s):  
Soundarya Priyadharsini K ◽  
Mali Kalpana Ramanna ◽  
Somu L ◽  
Krishna Prasad T
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Treated Group ◽  
Diet Group ◽  
Lipoprotein A ◽  
Group I ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Progression Of Atherosclerosis

Background Hypercholesterolemia is the predominantfactor in developing atherosclerosis and myocardial diseases.A major contributor for the progression of atherosclerosis is abnormalities in lipid and lipoprotein metabolism.Hence the objectives of the study was to estimatethe comparative efficacy of Lutein with atorvastatin on lipid profile and lipoprotein(a) and to estimate the histopahthological changes in hypercholesterolemic male wistar rats. Materials and Methods Experimental Wistar rats (male) were grouped into six. Each group contains 6 rats. Group I is control. Group II received cholesterol rich diet. Group III received cholesterol rich diet and the drug Atorvastatin 5mg/kg. Group IV received cholesterol rich diet and the drugLutein 25mg/kg. Group V received cholesterol rich diet and the drugLutein 50mg/kg. Group VI received cholesterol rich diet and the drug Lutein100mg/kg. At the end of 16 weeks, Blood samples from each rats was taken through retro-orbital puncture to evaluate serum lipoproteinsand lipoprotein(a) and thenwistar rats were sacrificed underinjection I.M Ketamine,Aortaand Liverwere dissected out and sent for histopathological studies. Results The plasma LDL, VLDL, Triglycerides, total cholesterol, lipoprotein(a) levels were reduced in all lutein treated groups and atorvastatin treated group compared to high cholesterol diet group. A significant rise in HDL levels was noted in all Lutein treated groups and atorvastatin treated group. No statistically significant difference was seen between Atorvastatin 5mg/kg body weight and Lutein 100mg/kg body weight on reduction of total cholesterol.The efficacy of the drug Lutein in progression of atherosclerosis and its cytoprotective action in liver was proved in this study. Conclusion This study indicates that Lutein has effect onreducing plasma lipoproteins&the study had shown significant antiatherogenic effect.

scholarly journals Evolution and Comparison Effects of Fludrocortisone and Betamethasone on Glucose and Lipid Profile in Rats

2020 ◽  
Author(s):  
Hamid Reza Jamshidi ◽  
Mohammad Naeem Malmir
Keyword(s):  
Blood Glucose ◽  
Side Effects ◽  
Lipid Profile ◽  
Intraperitoneal Injection ◽  
Wistar Rats ◽  
Daily Basis ◽  
Medical Sciences ◽  
Impaired Insulin ◽  
Improper Use ◽  
Male Wistar Rats

Hyperglycemia may associate with improper use of glucocorticoids, impaired insulin function, or both, and is associated with many complications such as hyperlipidemia and Hyperglycemia. Researches suggest that proper use of glucocorticoids can delay the onset and progression of complications of hyperglycemia and hyperlipidemia. In the present study, we compare two of these compounds on glucose and lipid profile level. We use 40 male Wistar rats from the Yazd Animal infertility center. Initially, the rats were randomly divided into 2 groups, and then each group was divided into 4 groups. Subsequently, fludrocortisone doses of 12, 24 and 36 mg/kg were administered to rats, and dosages of 6, 12 and 18 mg/kg for betamethasone administered to rats on a daily basis at 1 o'clock for 21 days by intraperitoneal injection. Betamethasone and Fludrocortisone increased blood glucose and AST, ALT, TG, LDL, VLDL, and decreased HDL, causing red pigmentation in the skin, and obesity and puffiness of the rats. In all of the measured factors, fludrocortisone changes were more than betamethasone. Fludrocortisone and betamethasone also had significant effects on weight, which was more pronounced with fludrocortisone. As the dose increased, the levels of AST, ALT, and cholesterol, TG, VLDL and LDL in the blood increased significantly and HDL levels decreased more in the blood, but fludrocortisone showed a stronger effect than betamethasone. Therefore, it can be expected that the use of Betamethasone would be logical due to fewer side effects than fludrocortisone. © 2019 Tehran University of Medical Sciences. All rights reserved. Acta Med Iran 2019;57(8):478-483.

Effect ofCitrus maximajuice on fasting blood glucose, lipid profile, liver enzyme and body weight

2018 ◽  
Vol 48 (5) ◽  
pp. 755-763 ◽  
Author(s):  
Peace Nwanneka Ani ◽  
Precious Chisom Aginam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Liver Enzyme ◽  
Wistar Rats ◽  
Fruit Juice ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Potential Contribution ◽  
Content Type

PurposeThis paper aims to investigate the effect ofCitrus maximajuice on fasting blood glucose, lipid profile, liver enzyme and body weight of alloxan-induced diabetic adult wistar rats.Design/methodology/approachThe fruits were thoroughly washed with potable water. They were peeled; the epicarp and seeds were removed. Fruit juice was extracted from the pulp. Animal experiment was carried out using 21 adult male wistar rats weighing about 110-130 g. The rats were categorized into three groups (A-C), with each group consisting of seven rats of similar body weights. Diabetes was induced using alloxan. Rats in Groups A and B were administered 300 mg and 600 mg ofC. maximafruit juice/kg body weight/day, respectively, for 14 days, while Group C rats acted as control and received placebo. Biochemical analysis was performed using standard procedures. Data were analyzed using SPSS, version 21.FindingsBlood glucose of rats in the treated groups decreased significantly (p< 0.05) from 454.7 and 569.7 mg/dl to 149.3 and 297.3 mg/dl, respectively, while the control increased from 257.0 to 46.57 mg/dl. Total cholesterol and triglyceride levels of Group B rats reduced significantly (p< 0.05), while the high-density lipoprotein cholesterol level increased (p< 0.05). Body weight of rats in the treated groups increased significantly (p< 0.05) by 30.1 and 20.2 per cent in Groups A and B, respectively, compared to the control group, which reduced by 0.21 per cent. There was no significant effect on markers of liver damage.Practical implicationsThe rate at which diabetes and other non-communicable diseases increase recently calls for affordable and sustainable management.Social implicationsDiabetes is one of the major health and development challenges of the 21st century, its complications are disabling and life threatening.Originality/valueThe hypoglycemic and hypocholesterolemic properties demonstrated byC. maximafruit juice suggest its potential contribution in reducing/stabilizing blood glucose level and managing complications of diabetes.

Sign in / Sign up

Export Citation Format

Share Document