scholarly journals Development of an automated on-chip bead-based ELISA platform

2015 ◽  
Vol 7 (19) ◽  
pp. 8472-8477 ◽  
Author(s):  
Jennifer Campbell ◽  
Nira R. Pollock ◽  
Andre Sharon ◽  
Alexis F. Sauer-Budge
Keyword(s):  
Immunosorbent Assay ◽  
Lab On A Chip ◽  
Enzyme Linked Immunosorbent Assay ◽  
Liquid Samples ◽  
On Chip

We present a lab-on-a-chip and associated instrument for heterogeneous enzyme-linked immunosorbent assay (ELISA)-based detection of proteins from liquid samples.

scholarly journals Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay

2014 ◽  
Vol 8 (6) ◽  
pp. 064101 ◽  
Author(s):  
Arnold Chen ◽  
Royal Wang ◽  
Candace R. S. Bever ◽  
Siyuan Xing ◽  
Bruce D. Hammock ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Lab On A Chip ◽  
Enzyme Linked Immunosorbent Assay

An integrated microfluidic system for on-chip enrichment and quantification of circulating extracellular vesicles from whole blood

Lab on a Chip ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 3305-3315 ◽  
Author(s):  
Yi-Sin Chen ◽  
Yu-Dong Ma ◽  
Chihchen Chen ◽  
Shu-Chu Shiesh ◽  
Gwo-Bin Lee
Keyword(s):  
Extracellular Vesicles ◽  
Immunosorbent Assay ◽  
Whole Blood ◽  
Magnetic Beads ◽  
Extracellular Vesicle ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Whole Blood ◽  
On Chip ◽  
Chip Enrichment

An integrated microfluidic system was developed for extracellular vesicle (EV) enrichment and quantification by using anti-CD63-coated magnetic beads and an on-chip enzyme-linked immunosorbent assay in human whole blood.

Ultrasensitive optofluidic enzyme-linked immunosorbent assay by on-chip integrated polymer whispering-gallery-mode microlaser sensors

Lab on a Chip ◽  
2020 ◽  
Vol 20 (14) ◽  
pp. 2438-2446 ◽  
Author(s):  
Xia Ouyang ◽  
Tong Liu ◽  
Yangxi Zhang ◽  
Jijun He ◽  
Zijian He ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Microfluidic Chip ◽  
Whispering Gallery Mode ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ultrasensitive Detection ◽  
Whispering Gallery ◽  
On Chip ◽  
Detection Of Biomarkers

Polymer whispering-gallery-mode microlaser sensors are optically 3D μ-printed and then integrated within a microfluidic chip for ultrasensitive detection of biomarkers.

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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