Ultrasensitive optofluidic enzyme-linked immunosorbent assay by on-chip integrated polymer whispering-gallery-mode microlaser sensors

Lab on a Chip ◽  
2020 ◽  
Vol 20 (14) ◽  
pp. 2438-2446 ◽  
Author(s):  
Xia Ouyang ◽  
Tong Liu ◽  
Yangxi Zhang ◽  
Jijun He ◽  
Zijian He ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Microfluidic Chip ◽  
Whispering Gallery Mode ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ultrasensitive Detection ◽  
Whispering Gallery ◽  
On Chip ◽  
Detection Of Biomarkers

Polymer whispering-gallery-mode microlaser sensors are optically 3D μ-printed and then integrated within a microfluidic chip for ultrasensitive detection of biomarkers.

scholarly journals On-Chip Glass Microspherical Shell Whispering Gallery Mode Resonators

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Chenchen Zhang ◽  
Alexander Cocking ◽  
Eugene Freeman ◽  
Zhiwen Liu ◽  
Srinivas Tadigadapa
Keyword(s):  
Whispering Gallery Mode ◽  
Whispering Gallery ◽  
On Chip

scholarly journals Modified ELISA for Ultrasensitive Diagnosis

2021 ◽  
Vol 10 (21) ◽  
pp. 5197
Author(s):  
Naoko Tsurusawa ◽  
Jyunhao Chang ◽  
Mayuri Namba ◽  
Daiki Makioka ◽  
Sou Yamura ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Present Article ◽  
Immunosorbent Assay ◽  
Current Literature ◽  
Quantitative Measurement ◽  
Review Article ◽  
Detection Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ultrasensitive Detection ◽  
Lifestyle Related Diseases

An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestyle-related diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.

An integrated microfluidic system for on-chip enrichment and quantification of circulating extracellular vesicles from whole blood

Lab on a Chip ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 3305-3315 ◽  
Author(s):  
Yi-Sin Chen ◽  
Yu-Dong Ma ◽  
Chihchen Chen ◽  
Shu-Chu Shiesh ◽  
Gwo-Bin Lee
Keyword(s):  
Extracellular Vesicles ◽  
Immunosorbent Assay ◽  
Whole Blood ◽  
Magnetic Beads ◽  
Extracellular Vesicle ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Whole Blood ◽  
On Chip ◽  
Chip Enrichment

An integrated microfluidic system was developed for extracellular vesicle (EV) enrichment and quantification by using anti-CD63-coated magnetic beads and an on-chip enzyme-linked immunosorbent assay in human whole blood.

On-chip single-mode high-Q terahertz whispering gallery mode resonator

2019 ◽  
Vol 44 (11) ◽  
pp. 2835 ◽  
Author(s):  
Ziwei Wang ◽  
Shixing Yuan ◽  
Gaoneng Dong ◽  
Ruolan Wang ◽  
Liao Chen ◽  
...  
Keyword(s):  
Single Mode ◽  
Whispering Gallery Mode ◽  
High Q ◽  
Whispering Gallery ◽  
On Chip ◽  
Whispering Gallery Mode Resonator ◽  
Mode Resonator

scholarly journals Development of an automated on-chip bead-based ELISA platform

2015 ◽  
Vol 7 (19) ◽  
pp. 8472-8477 ◽  
Author(s):  
Jennifer Campbell ◽  
Nira R. Pollock ◽  
Andre Sharon ◽  
Alexis F. Sauer-Budge
Keyword(s):  
Immunosorbent Assay ◽  
Lab On A Chip ◽  
Enzyme Linked Immunosorbent Assay ◽  
Liquid Samples ◽  
On Chip

We present a lab-on-a-chip and associated instrument for heterogeneous enzyme-linked immunosorbent assay (ELISA)-based detection of proteins from liquid samples.

Optical Whispering-Gallery Mode Phenomenon as a Composite Sensor With Applications to Direct On-Chip Thermal Sensing

2013 ◽  
Author(s):  
Matthew Frenkel ◽  
Marlon Avellan ◽  
Zhixiong Guo
Keyword(s):  
Composite System ◽  
Whispering Gallery Mode ◽  
Quality Factors ◽  
Pressure Sensing ◽  
Whispering Gallery ◽  
Area Of Interest ◽  
Thermal Sensing ◽  
On Chip ◽  
Wgm Resonators ◽  
Very High

Optical Whispering-Gallery Mode (WGM) resonators can be fabricated with very high quality factors allowing for their use as high resolution sensors in a myriad of fields ranging from quantum electro-dynamics (QED) to pressure sensing. In this paper, we focus on integrating WGM as a dynamic temperature measurement device. The WGM sensors are fabricated onto the heating element, instead of acting as an indirect temperature sensor, allowing for direct monitoring of an area of interest. An adaptation to the WGM theoretical model, to include the thermal expansion of the composite system, is discussed and analyzed.

scholarly journals Detection of Plasmodium Aldolase Using a Smartphone and Microfluidic Enzyme Linked Immunosorbent Assay

2017 ◽  
Vol 2017 ◽  
pp. 1-4
Author(s):  
Nikhil S. Gopal ◽  
Ruben Raychaudhuri
Keyword(s):  
Immunosorbent Assay ◽  
Microfluidic Chip ◽  
Rural Areas ◽  
Color Change ◽  
Low Cost ◽  
Sandwich Elisa ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cost System ◽  
Therapeutic Outcomes

Background. Malaria control efforts are limited in rural areas. A low-cost system to monitor response without the use of electricity is needed. Plasmodium aldolase is a malaria biomarker measured using enzyme linked immunosorbent assay (ELISA) techniques. A three-part system using ELISA was developed consisting of a microfluidic chip, hand crank centrifuge, and a smartphone. Methods. A circular microfluidic chip was fabricated using clear acrylic and a CO2 laser. A series of passive valves released reagents at precise times based upon centrifugal force. Color change was measured via smartphone camera using an application programmed in Java. The microchip was compared to a standard 96-well sandwich ELISA. Results. Results from standard ELISA were compared to microchip at varying concentrations (1–10 ng/mL). Over 15 different microfluidic patterns were tested, and a final prototype of the chip was created. The prototype microchip was compared to standard sandwich ELISA (n=20) using samples of recombinant aldolase. Color readings of standard ELISA and microfluidic microchip showed similar results. Conclusion. A low-cost microfluidic system could detect and follow therapeutic outcomes in rural areas and identify resistant strains.

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