One-step synthesis of cellulose/silver nanobiocomposites using a solution plasma process and characterization of their broad spectrum antimicrobial efficacy

RSC Advances ◽  
2015 ◽  
Vol 5 (44) ◽  
pp. 35052-35060 ◽  
Author(s):  
MubarakAli Davoodbasha ◽  
Sang-Yul Lee ◽  
Seong-Cheol Kim ◽  
Jung-Wan Kim
Keyword(s):  
Antimicrobial Activity ◽  
Broad Spectrum ◽  
Antimicrobial Efficacy ◽  
Human Pathogens ◽  
Plasma Process ◽  
One Step

Cellulose/AgNP biocomposites were synthesized using SPP and assessed for their antimicrobial activity against several human pathogens.

scholarly journals Production of an Engineered Killer Peptide in Nicotiana benthamiana by Using a Potato virus X Expression System

2005 ◽  
Vol 71 (10) ◽  
pp. 6360-6367 ◽  
Author(s):  
Marcello Donini ◽  
Chiara Lico ◽  
Selene Baschieri ◽  
Stefania Conti ◽  
Walter Magliani ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pseudomonas Syringae ◽  
Potato Virus ◽  
Broad Spectrum ◽  
Plant Pathogens ◽  
Expression System ◽  
Potato Virus X ◽  
Scale Production ◽  
Human Pathogens ◽  
Single Chain

ABSTRACT The decapeptide killer peptide (KP) derived from the sequence of a single-chain, anti-idiotypic antibody acting as a functional internal image of a microbicidal, broad-spectrum yeast killer toxin (KT) was shown to exert a strong microbicidal activity against human pathogens. With the aim to exploit this peptide to confer resistance to plant pathogens, we assayed its antimicrobial activity against a broad spectrum of phytopathogenic bacteria and fungi. Synthetic KP exhibited antimicrobial activity in vitro towards Pseudomonas syringae, Erwinia carotovora, Botrytis cinerea, and Fusarium oxysporum. KP was also expressed in plants by using a Potato virus X (PVX)-derived vector as a fusion to the viral coat protein, yielding chimeric virus particles (CVPs) displaying the heterologous peptide. Purified CVPs showed enhanced antimicrobial activity against the above-mentioned plant pathogens and human pathogens such as Staphylococcus aureus and Candida albicans. Moreover, in vivo assays designed to challenge KP-expressing plants (as CVPs) with Pseudomonas syringae pv. tabaci showed enhanced resistance to bacterial attack. The results indicate that the PVX-based display system is a high-yield, rapid, and efficient method to produce and evaluate antimicrobial peptides in plants, representing a milestone for the large-scale production of high-added-value peptides through molecular farming. Moreover, KP is a promising molecule to be stably engineered in plants to confer broad-spectrum resistance to phytopathogens.

Gene isolation and structural characterization of a legume tree defensin with a broad spectrum of antimicrobial activity

Planta ◽  
2019 ◽  
Vol 250 (5) ◽  
pp. 1757-1772 ◽  
Author(s):  
Susana Rodríguez-Decuadro ◽  
Pablo D. Dans ◽  
María Alejandra Borba ◽  
Ana Maria Benko-Iseppon ◽  
Gianna Cecchetto
Keyword(s):  
Antimicrobial Activity ◽  
Structural Characterization ◽  
Broad Spectrum ◽  
Gene Isolation ◽  
Legume Tree

Characterization of a type-I crustin with broad-spectrum antimicrobial activity from red swamp crayfish Procambarus clarkii

2016 ◽  
Vol 61 ◽  
pp. 145-153 ◽  
Author(s):  
Ning Liu ◽  
Ran-Ran Zhang ◽  
Zhen-Xu Fan ◽  
Xiao-Fan Zhao ◽  
Xian-Wei Wang ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Broad Spectrum ◽  
Procambarus Clarkii ◽  
Type I ◽  
Red Swamp Crayfish

scholarly journals Ozonolysis of neem oil: preparation and characterization of potent antibacterial agents against multidrug resistant bacterial strains

RSC Advances ◽  
2017 ◽  
Vol 7 (55) ◽  
pp. 34356-34365 ◽  
Author(s):  
Paola de Oliveira ◽  
Nathália de Almeida ◽  
Martin Conda-Sheridan ◽  
Rafael do Prado Apparecido ◽  
Ana Camila Micheletti ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Broad Spectrum ◽  
Antibacterial Agents ◽  
Multidrug Resistant ◽  
Neem Oil ◽  
Bacterial Strains ◽  
Vancomycin Resistant

Neem ozonated oils showed excellent broad-spectrum antimicrobial activity against standard E. faecalis, clinical vancomycin resistant E. faecium, clinical multiresistant K. pneumoniae (KPC), and S. aureus (MRSA and standard).

scholarly journals A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil BacteriumBacillussp. YAS 1 Strain

2014 ◽  
Vol 2014 ◽  
pp. 1-16 ◽  
Author(s):  
Amira M. Embaby ◽  
Yasmin Heshmat ◽  
Ahmed Hussein ◽  
Heba S. Marey
Keyword(s):  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Statistical Approach ◽  
Wide Spectrum ◽  
Enterobacter Aerogenes ◽  
Lactobacillus Bulgaricus ◽  
Human Pathogens ◽  
Wide Range ◽  
Potential Applications

Bacteriocins, ribosomally synthesized antimicrobial peptides, display potential applications in agriculture, medicine, and industry. The present study highlights integral statistical optimization and partial characterization of a bacteriocin substance from a soil bacterium taxonomically affiliated asBacillussp. YAS 1 after biochemical and molecular identifications. A sequential statistical approach (Plackett-Burman and Box-Behnken) was employed to optimize bacteriocin (BAC YAS 1) production. Using optimal levels of three key determinants (yeast extract (0.48% (w/v), incubation time (62 hrs), and agitation speed (207 rpm)) in peptone yeast beef based production medium resulted in 1.6-fold enhancement in BAC YAS 1 level (470 AU/mL arbitrary units againstErwinia amylovora). BAC YAS 1 showed activity over a wide range of pH (1–13) and temperature (45–80°C). A wide spectrum antimicrobial activity of BAC YAS 1 against the human pathogens (Clostridium perfringens,Staphylococcus epidermidis,Campylobacter jejuni,Enterobacter aerogenes,Enterococcussp.,Proteussp.,Klebsiellasp., andSalmonella typhimurium), the plant pathogen (E. amylovora), and the food spoiler (Listeria innocua) was demonstrated. On top and above, BAC YAS 1 showed no antimicrobial activity towards lactic acid bacteria (Lactobacillus bulgaricus,L. casei,L. lactis, andL. reuteri). Promising characteristics of BAC YAS 1 prompt its commercialization for efficient utilization in several industries.

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

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