Hyaluronic acid-functionalized electrospun PLGA nanofibers embedded in a microfluidic chip for cancer cell capture and culture

2017 ◽  
Vol 5 (4) ◽  
pp. 752-761 ◽  
Author(s):  
Gangwei Xu ◽  
Yulong Tan ◽  
Tiegang Xu ◽  
Di Yin ◽  
Mengyuan Wang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Microfluidic Chip ◽  
Cell Capture

Hyaluronic acid-functionalized electrospun PLGA nanofibers embedded in a microfluidic chip are able to effectively capture cancer cells.

MICROFLUIDIC CHIP FOR CANCER CELL DETECTION AND DIAGNOSIS

2018 ◽  
Vol 18 (01) ◽  
pp. 1830001 ◽  
Author(s):  
CHIYU LI ◽  
WANG LI ◽  
CHUNYANG GENG ◽  
HAIJUN REN ◽  
XIAOHUI YU ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Microfluidic Chip ◽  
Cell Biology ◽  
Cell Detection ◽  
Microfluidic Chips ◽  
Cell Capture ◽  
Unique Method ◽  
Detection And Diagnosis ◽  
Cancer Cell Detection

Since cancer becomes the most deadly disease to our health, research on early detection on cancer cells is necessary for clinical treatment. The combination of microfluidic device with cell biology has shown a unique method for cancer cell research. In the present review, recent development on microfluidic chip for cancer cell detection and diagnosis will be addressed. Some typical microfluidic chips focussed on cancer cells and their advantages for different kinds of cancer cell detection and diagnosis will be listed, and the cell capture methods within the microfluidics will be simultaneously mentioned. Then the potential direction of microfluidic chip on cancer cell detection and diagnosis in the future is also discussed.

Bladder Cancer Cell Capture: Elucidating the Effect of Sample Storage Conditions on Capturing Bladder Cancer Cells via Surface Immobilized EpCAM Antibody

2019 ◽  
Vol 2 (9) ◽  
pp. 3730-3736 ◽  
Author(s):  
Kola Ostrikov ◽  
Thomas Michl ◽  
Melanie MacGregor ◽  
Krasimir Vasilev
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Storage Conditions ◽  
Bladder Cancer Cell ◽  
Cell Capture ◽  
Sample Storage ◽  
Bladder Cancer Cells

scholarly journals Physical and Biological Evaluation of Low-Molecular-Weight Hyaluronic Acid/Fe3O4 Nanoparticle for Targeting MCF7 Breast Cancer Cells

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1094 ◽  
Author(s):  
Hsin-Ta Wang ◽  
Po-Chien Chou ◽  
Ping-Han Wu ◽  
Chi-Ming Lee ◽  
Kang-Hsin Fan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Breast Cancer Cells ◽  
Low Molecular Weight ◽  
Cell Targeting ◽  
Fe3o4 Nps ◽  
Targeting Ability

Low-molecular-weight hyaluronic acid (LMWHA) was integrated with superparamagnetic Fe3O4 nanoparticles (Fe3O4 NPs). The size distribution, zeta potential, viscosity, thermogravimetric and paramagnetic properties of the LMWHA-Fe3O4 NPs were systematically examined. For cellular experiments, MCF7 breast cancer cell line was carried out. In addition, the cell targeting ability and characteristics of the LMWHA-Fe3O4 NPs for MCF7 breast cancer cells were analyzed using the thiocyanate method and time-of-flight secondary ion mass spectrometry (TOF-SIMS). The experimental results showed that the LMWHA-Fe3O4 NPs were not only easily injectable due to their low viscosity, but also exhibited a significant superparamagnetic property. Furthermore, the in vitro assay results showed that the NPs had negligible cytotoxicity and exhibited a good cancer cell targeting ability. Overall, the results therefore suggest that the LMWHA-Fe3O4 NPs have considerable potential as an injectable agent for enhanced magnetic resonance imaging (MRI) and/or hyperthermia treatment in breast cancer therapy.

Hyaluronic Acid-Functionalized Electrospun Polyvinyl Alcohol/Polyethyleneimine Nanofibers for Cancer Cell Capture Applications

2015 ◽  
Vol 2 (15) ◽  
pp. 1500256 ◽  
Author(s):  
Yili Zhao ◽  
Zhangyu Fan ◽  
Mingwu Shen ◽  
Xiangyang Shi
Keyword(s):  
Hyaluronic Acid ◽  
Polyvinyl Alcohol ◽  
Cancer Cell ◽  
Cell Capture

Dendrimer-functionalized electrospun cellulose acetate nanofibers for targeted cancer cell capture applications

2014 ◽  
Vol 2 (42) ◽  
pp. 7384-7393 ◽  
Author(s):  
Yili Zhao ◽  
Xiaoyue Zhu ◽  
Hui Liu ◽  
Yu Luo ◽  
Shige Wang ◽  
...  
Keyword(s):  
Folic Acid ◽  
Cancer Cells ◽  
Cellulose Acetate ◽  
Cancer Cell ◽  
Cell Capture

Multifunctional folic acid-functionalized dendrimers can be modified on the surface of electrospun cellulose acetate nanofibers for the specific capture of FAR-overexpressing cancer cells.

Coordination-driven reversible surfaces with site-specifically immobilized nanobody for dynamic cancer cell capture and release

2020 ◽  
Vol 8 (33) ◽  
pp. 7511-7520
Author(s):  
Lulu Han ◽  
Ruilian Peng ◽  
Wenning Jiang ◽  
Ting Xu ◽  
Chong Zhang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
High Efficiency ◽  
Cell Capture ◽  
Capture And Release

Coordination-driven reversible surfaces with site-specifically immobilized his-tagged nanobody for high-efficiency capture and release of cancer cells.

Selective Breast Cancer Cell Capture, Culture, and Immunocytochemical Analysis Using Self-Assembled Magnetic Bead Patterns in a Microfluidic Chip

Langmuir ◽  
2010 ◽  
Vol 26 (9) ◽  
pp. 6091-6096 ◽  
Author(s):  
Venkataragavalu Sivagnanam ◽  
Bo Song ◽  
Caroline Vandevyver ◽  
Jean-Claude G. Bünzli ◽  
Martin A. M. Gijs
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Microfluidic Chip ◽  
Magnetic Bead ◽  
Cell Capture ◽  
Immunocytochemical Analysis ◽  
Self Assembled

Direct photo-patterning of hyaluronic acid baits onto a fouling-release perfluoropolyether surface for selective cancer cell capture and immobilization

2016 ◽  
Vol 62 ◽  
pp. 414-422 ◽  
Author(s):  
Caterina Credi ◽  
Carmela De Marco ◽  
Elena Molena ◽  
Michele M. Nava ◽  
Manuela T. Raimondi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cancer Cell ◽  
Cell Capture ◽  
Fouling Release

Cancer cell–nanomaterial interface: role of geometry and surface charge of nanocomposites in the capture efficiency and cell viability

2019 ◽  
Vol 7 (7) ◽  
pp. 2759-2768 ◽  
Author(s):  
Yishu He ◽  
Jingwen Qin ◽  
Shengming Wu ◽  
Haocheng Yang ◽  
Huiyun Wen ◽  
...  
Keyword(s):  
Cell Viability ◽  
Surface Charge ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Capture Efficiency ◽  
Experimental Results ◽  
Magnetic Nanocomposites ◽  
Cell Capture ◽  
Cell Interface

The nanomaterial–cell interface plays an important role in biodetection and therapy. The experimental results in this study indicated that the magnetic nanocomposites with strong positive surface charge but different geometry interacted with cancer cells in different ways, leading to various cell capture efficiency and cytotoxicity.

Cancer Cell Surface Negative Charges: A Bio-Physical Manifestation of the Warburg Effect

Nano LIFE ◽  
2017 ◽  
Vol 07 (03n04) ◽  
pp. 1771001 ◽  
Author(s):  
Donglu Shi
Keyword(s):  
Early Detection ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Cell ◽  
Cell Detection ◽  
Cell Capture ◽  
Metabolic Pattern ◽  
Early Cancer Diagnosis ◽  
Highly Sensitive

The early detection of circulating tumor cells (CTCs) in blood as part of medical diagnosis will give the doctors a head start in the provision and treatment of cancer, and therefore, with the advance in Nano technology, there is an increasing expectation of some form of early detection of circulating tumor cells at a highly sensitive level, without any biomarkers, for both early cancer diagnosis and monitoring disease progression after medical intervention. This technical note reports on the recent development in detection of highly sensitive detection of cancer cells without biomarkers. This novel concept is developed based on a hallmark cancer metabolic pattern: high glycolysis rate. Secretion of high level of lactate acid by cancer cells ultimately results in negative electrical charges on their surfaces, enabling strong binding and capturing by the positively-charged nanoprobes, and subsequent magnetic separation. When nanoprobes are incubated with cancer cells in suspension, binding takes place due to charge differences, and cancer cells are then magnetically separated. The separated cells are enumerated using a flow cytometry and identified by pathological and genome sequencing methods. Preliminary results using the approach have shown exceptionally high cancer cell capture rates, therefore potentially applicable in cancer cell detection in clinical settings.

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