Coordination-driven reversible surfaces with site-specifically immobilized nanobody for dynamic cancer cell capture and release

2020 ◽  
Vol 8 (33) ◽  
pp. 7511-7520
Author(s):  
Lulu Han ◽  
Ruilian Peng ◽  
Wenning Jiang ◽  
Ting Xu ◽  
Chong Zhang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
High Efficiency ◽  
Cell Capture ◽  
Capture And Release

Coordination-driven reversible surfaces with site-specifically immobilized his-tagged nanobody for high-efficiency capture and release of cancer cells.

A Magnetic Dynamic Microbiointerface with Biofeedback Mechanism for Cancer Cell Capture and Release

2019 ◽  
Vol 11 (44) ◽  
pp. 41019-41029 ◽  
Author(s):  
Xiaohua Tian ◽  
Xiangyu Sha ◽  
Yonghai Feng ◽  
Yuqing Duan ◽  
Mingdong Dong ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Cell Capture ◽  
Magnetic Dynamic ◽  
Capture And Release

scholarly journals An Anti-Cancer Peptide LVTX-8 Inhibits the Proliferation and Migration of Lung Tumor Cells by Regulating Causal Genes’ Expression in p53-Related Pathways

Toxins ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 367
Author(s):  
Peng Zhang ◽  
Yujie Yan ◽  
Junting Wang ◽  
Xiaoping Dong ◽  
Gaihua Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Growth ◽  
Cancer Cells ◽  
Cancer Cell ◽  
High Efficiency ◽  
Lung Tumor ◽  
Lung Cancer Cells ◽  
Helical Conformation ◽  
Anti Cancer ◽  
And Migration

Spider venom has been found to show its anticancer activity in a variety of human malignancies, including lung cancer. In this study, we investigated the anti-cancer peptide toxin LVTX-8, with linear amphipathic alpha-helical conformation, designed and synthesized from the cDNA library of spider Lycosa vittata. Multiple cellular methods, such as CCK-8 assay, flow cytometry, colony formation assay, Transwell invasion and migration assay, were performed to detect peptide-induced cell growth inhibition and anti-metastasis in lung cancer cells. Our results demonstrated that LVTX-8 displayed strong cytotoxicity and anti-metastasis towards lung cancer in vitro. Furthermore, LVTX-8 could suppress the growth and metastasis of lung cancer cells (A549 and H460) in nude mouse models. Transcriptomics, integrated with multiple bioinformatics analysis, suggested that the molecular basis of the LVTX-8-mediated inhibition of cancer cell growth and metastasis manifested in two aspects: Firstly, it could restrain the activity of cancer cell division and migration through the functional pathways, including “p53 hypoxia pathway” and “integrin signaling”. Secondly, it could regulate the expression level of apoptotic-related proteins, which may account for programmed apoptosis of cancer cells. Taken together, as an anticancer peptide with high efficiency and acceptable specificity, LVTX-8 may become a potential precursor of a therapeutic agent for lung cancer in the future.

Hyaluronic acid-functionalized electrospun PLGA nanofibers embedded in a microfluidic chip for cancer cell capture and culture

2017 ◽  
Vol 5 (4) ◽  
pp. 752-761 ◽  
Author(s):  
Gangwei Xu ◽  
Yulong Tan ◽  
Tiegang Xu ◽  
Di Yin ◽  
Mengyuan Wang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Microfluidic Chip ◽  
Cell Capture

Hyaluronic acid-functionalized electrospun PLGA nanofibers embedded in a microfluidic chip are able to effectively capture cancer cells.

Dendrimer-functionalized electrospun cellulose acetate nanofibers for targeted cancer cell capture applications

2014 ◽  
Vol 2 (42) ◽  
pp. 7384-7393 ◽  
Author(s):  
Yili Zhao ◽  
Xiaoyue Zhu ◽  
Hui Liu ◽  
Yu Luo ◽  
Shige Wang ◽  
...  
Keyword(s):  
Folic Acid ◽  
Cancer Cells ◽  
Cellulose Acetate ◽  
Cancer Cell ◽  
Cell Capture

Multifunctional folic acid-functionalized dendrimers can be modified on the surface of electrospun cellulose acetate nanofibers for the specific capture of FAR-overexpressing cancer cells.

MICROFLUIDIC CHIP FOR CANCER CELL DETECTION AND DIAGNOSIS

2018 ◽  
Vol 18 (01) ◽  
pp. 1830001 ◽  
Author(s):  
CHIYU LI ◽  
WANG LI ◽  
CHUNYANG GENG ◽  
HAIJUN REN ◽  
XIAOHUI YU ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Microfluidic Chip ◽  
Cell Biology ◽  
Cell Detection ◽  
Microfluidic Chips ◽  
Cell Capture ◽  
Unique Method ◽  
Detection And Diagnosis ◽  
Cancer Cell Detection

Since cancer becomes the most deadly disease to our health, research on early detection on cancer cells is necessary for clinical treatment. The combination of microfluidic device with cell biology has shown a unique method for cancer cell research. In the present review, recent development on microfluidic chip for cancer cell detection and diagnosis will be addressed. Some typical microfluidic chips focussed on cancer cells and their advantages for different kinds of cancer cell detection and diagnosis will be listed, and the cell capture methods within the microfluidics will be simultaneously mentioned. Then the potential direction of microfluidic chip on cancer cell detection and diagnosis in the future is also discussed.

Cancer cell–nanomaterial interface: role of geometry and surface charge of nanocomposites in the capture efficiency and cell viability

2019 ◽  
Vol 7 (7) ◽  
pp. 2759-2768 ◽  
Author(s):  
Yishu He ◽  
Jingwen Qin ◽  
Shengming Wu ◽  
Haocheng Yang ◽  
Huiyun Wen ◽  
...  
Keyword(s):  
Cell Viability ◽  
Surface Charge ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Capture Efficiency ◽  
Experimental Results ◽  
Magnetic Nanocomposites ◽  
Cell Capture ◽  
Cell Interface

The nanomaterial–cell interface plays an important role in biodetection and therapy. The experimental results in this study indicated that the magnetic nanocomposites with strong positive surface charge but different geometry interacted with cancer cells in different ways, leading to various cell capture efficiency and cytotoxicity.

Cancer Cell Surface Negative Charges: A Bio-Physical Manifestation of the Warburg Effect

Nano LIFE ◽  
2017 ◽  
Vol 07 (03n04) ◽  
pp. 1771001 ◽  
Author(s):  
Donglu Shi
Keyword(s):  
Early Detection ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Cell ◽  
Cell Detection ◽  
Cell Capture ◽  
Metabolic Pattern ◽  
Early Cancer Diagnosis ◽  
Highly Sensitive

The early detection of circulating tumor cells (CTCs) in blood as part of medical diagnosis will give the doctors a head start in the provision and treatment of cancer, and therefore, with the advance in Nano technology, there is an increasing expectation of some form of early detection of circulating tumor cells at a highly sensitive level, without any biomarkers, for both early cancer diagnosis and monitoring disease progression after medical intervention. This technical note reports on the recent development in detection of highly sensitive detection of cancer cells without biomarkers. This novel concept is developed based on a hallmark cancer metabolic pattern: high glycolysis rate. Secretion of high level of lactate acid by cancer cells ultimately results in negative electrical charges on their surfaces, enabling strong binding and capturing by the positively-charged nanoprobes, and subsequent magnetic separation. When nanoprobes are incubated with cancer cells in suspension, binding takes place due to charge differences, and cancer cells are then magnetically separated. The separated cells are enumerated using a flow cytometry and identified by pathological and genome sequencing methods. Preliminary results using the approach have shown exceptionally high cancer cell capture rates, therefore potentially applicable in cancer cell detection in clinical settings.

N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Lisni Noraida Waruwu ◽  
Maria Bintang ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Green Tea ◽  
Cancer Cell ◽  
Breast Cancer Cells ◽  
Green Tea Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Tea Extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

Synthesis and Characterization of Quinoline-3-Carboxamide Derivatives as Inhibitors of the ATM Kinase

2020 ◽  
Vol 20 (23) ◽  
pp. 2070-2079
Author(s):  
Srimadhavi Ravi ◽  
Sugata Barui ◽  
Sivapriya Kirubakaran ◽  
Parul Duhan ◽  
Kaushik Bhowmik
Keyword(s):  
Western Blot ◽  
Cancer Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Cancer Cell Lines ◽  
Atm Kinase ◽  
Cytotoxicity Studies

Background: The importance of inhibiting the kinases of the DDR pathway for radiosensitizing cancer cells is well established. Cancer cells exploit these kinases for their survival, which leads to the development of resistance towards DNA damaging therapeutics. Objective: In this article, the focus is on targeting the key mediator of the DDR pathway, the ATM kinase. A new set of quinoline-3-carboxamides, as potential inhibitors of ATM, is reported. Methods: Quinoline-3-carboxamide derivatives were synthesized and cytotoxicity assay was performed to analyze the effect of molecules on different cancer cell lines like HCT116, MDA-MB-468, and MDA-MB-231. Results: Three of the synthesized compounds showed promising cytotoxicity towards a selected set of cancer cell lines. Western Blot analysis was also performed by pre-treating the cells with quercetin, a known ATM upregulator, by causing DNA double-strand breaks. SAR studies suggested the importance of the electron-donating nature of the R group for the molecule to be toxic. Finally, Western-Blot analysis confirmed the down-regulation of ATM in the cells. Additionally, the PTEN negative cell line, MDA-MB-468, was more sensitive towards the compounds in comparison with the PTEN positive cell line, MDA-MB-231. Cytotoxicity studies against 293T cells showed that the compounds were at least three times less toxic when compared with HCT116. Conclusion: In conclusion, these experiments will lay the groundwork for the evolution of potent and selective ATM inhibitors for the radio- and chemo-sensitization of cancer cells.

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